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Experimental Increase (experimental + increase)
Selected AbstractsThe proto-oncogene BCL6 promotes survival of olfactory sensory neuronsDEVELOPMENTAL NEUROBIOLOGY, Issue 6 2010Joji M. Otaki Abstract For the mammalian olfactory epithelium to continually detect odorant, neuronal survival, apoptosis, and regeneration must be coordinated. Here, we showed that the proto-oncogene BCL6, which encodes a transcriptional repressor required for lymphocyte terminal differentiation, contributes to the survival of olfactory sensory neurons (OSNs). In the olfactory epithelia of the BCL6 null mutant mice, many OSNs were positive for both OMP and GAP43. The epithelium was relatively thinner, showing many apoptotic signals. These characters were phenotypically similar to those of the wild-type mice treated with nasal lectin irrigation, which acutely induces apoptosis of OSNs. Odorant receptors were expressed normally in the epithelia of the mutant mice, and their overall expression profile based on DNA microarray analyses was roughly similar to that of the apoptosis-induced olfactory epithelia of the wild-type mice. Experimental increase of BCL6 together with green fluorescent protein in OSNs using adenovirus-mediated gene transfer made the epifluorescence last longer than the control fluorescence without exogenous BCL6 after the nasal lectin irrigation, indicating that BCL6 made the infected neurons survive longer. We conclude that BCL6 plays an active role in the survival of OSNs as an anti-apoptotic factor and confers immature OSNs enough time to fully differentiate into mature ones. © 2010 Wiley Periodicals, Inc. Develop Neurobiol 70: 424-435, 2010 [source] Smaller and more numerous harvesting gaps emulate natural forest disturbances: a biodiversity test case using rove beetles (Coleoptera, Staphylinidae)DIVERSITY AND DISTRIBUTIONS, Issue 6 2008Jan Klimaszewski ABSTRACT Aim To evaluate changes in the abundance, species richness and community composition of rove beetles (Coleoptera, Staphylinidae) in response to three configurations of experimental gap cuts and to the effects of ground scarification in early succession yellow birch-dominated boreal forest. In each experimental treatment, total forest removed was held constant (35% removal by partial cutting with a concomitant decrease in gap size) but the total number of gaps was increased (two, four and eight gaps, respectively), resulting in an experimental increase in the total amount of ,edge' within each stand. Location Early succession yellow birch-dominated forests, Quebec, Canada. Methods Pitfall traps, ANOVA, MIXED procedure in sas®, post hoc Tukey's adjustment, rarefaction estimates, sum-of-squares and distance-based multivariate regression trees (ssMRT, dbMRT). Results Estimates of species richness using rarefaction were highest in clearcut and two-gap treatments, decreased in smaller and more numerous gaps and were significantly higher in scarified areas than in unscarified areas. ANOVA indicated a significant impact of harvesting on the overall standardized catch. Post hoc Tukey's tests indicated that the total catch of all rove beetles was significantly higher in uncut forests than in the treated areas. Both sum-of-squares and distance-based multivariate regression trees indicated that community structure of rove beetles differed among treatments. Assemblages were grouped into (a) control plots, (b) four- and eight-gap treatments and (c) two-gap and clearcut treatments. Main conclusions Rove beetle composition responded significantly to increasing gap size. Composition among intermediate and small-sized gap treatments (four- and eight-gap treatments) was more similar to uncut control forests than were larger gap treatments (two-gap) and clearcuts. Effects of scarification were nested within the harvested treatments. When the total area of forest removed is held constant, smaller, more numerous gaps are more similar to uncut control stands than to larger gaps and falls more closely within the natural forest heterogeneity. [source] Consequences of simultaneous elevation of carbon dioxide and temperature for plant,herbivore interactions: a metaanalysisGLOBAL CHANGE BIOLOGY, Issue 1 2006E. L. ZVEREVA Abstract The effects of elevated carbon dioxide on plant,herbivore interactions have been summarized in a number of narrative reviews and metaanalyses, while accompanying elevation of temperature has not received sufficient attention. The goal of our study is to search, by means of metaanalysis, for a general pattern in responses of herbivores, and plant characteristics important for herbivores, to simultaneous experimental increase of carbon dioxide and temperature (ECET) in comparison with both ambient conditions and responses to elevated CO2 (EC) and temperature (ET) applied separately. Our database includes 42 papers describing studies of 31 plant species and seven herbivore species. Nitrogen concentration and C/N ratio in plants decreased under both EC and ECET treatments, whereas ET had no significant effect. Concentrations of nonstructural carbohydrates and phenolics increased in EC, decreased in ET and did not change in ECET treatments, whereas terpenes did not respond to EC but increased in both ET and ECET; leaf toughness increased in both EC and ECET. Responses of defensive secondary compounds to treatments differed between woody and green tissues as well as between gymnosperm and angiosperm plants. Insect herbivore performance was adversely affected by EC, favoured by ET, and not modified by ECET. Our analysis allowed to distinguish three types of relationships between CO2 and temperature elevation: (1) responses to EC do not depend on temperature (nitrogen, C/N, leaf toughness, phenolics in angiosperm leaves), (2) responses to EC are mitigated by ET (sugars and starch, terpenes in needles of gymnosperms, insect performance) and (3) effects emerge only under ECET (nitrogen in gymnosperms, and phenolics and terpenes in woody tissues). This result indicates that conclusions of CO2 elevation studies cannot be directly extrapolated to a more realistic climate change scenario. The predicted negative effects of CO2 elevation on herbivores are likely to be mitigated by temperature increase. [source] Complex regulation of choroidal blood flow during combined changes in blood pressure and IOPACTA OPHTHALMOLOGICA, Issue 2009L SCHMETTERER Purpose In the recent years it has been shown that the human choroid shows some regulatory capacity during an artifical increase in intraocular pressure (IOP) as well as during an exercise induced increase in mean arterial pressure (MAP). The purpose of the present study was to test the hypothesis that human choroidal blood flow (ChBF) may depend, not only on ocular perfusion pressure (OPP), but also on absolute levels of MAP and IOP. Methods In a clinical study in 28 helathy subjects OPP was varied by elevating IOP during a squatting-induced increase in MAP. IOP was raised in stepwise increments by using the suction cup method. Subfoveal choroidal blood flow (VhBF, laser Doppler flowmetry), MAP, and IOP were assessed, and OPP was calculated as (2/3)(MAP - IOP). For correlation analysis, data from all subjects were pooled according to IOP and MAP values, and correlation analyses were performed. Results When data were grouped according to IOP, no correlation was observed between ChBF and MAP; but ChBFs were lower, the higher the IOP (P < 0.001). When data were grouped according to MAP, a significant correlation was found between ChBF and IOP (P < 0.001), but correlations were independent of MAP. Conclusion These data confirm previously published observations that the choroid shows some regulatory capacity during changes in OPP. In addition, the data indicate that the choroid regulates its blood flow better during exercise-induced changes in MAP than during an experimental increase in IOP. [source] | ||||||||||