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Selected AbstractsBONDSYM: SIMULINK-based educational software for analysis of dynamic systemCOMPUTER APPLICATIONS IN ENGINEERING EDUCATION, Issue 2 2010J.A. Calvo Abstract This article presents an educational software called BONDSYM developed to allow engineering students to learn easily and quickly about the analysis of dynamic systems through the Bond Graph method. This software uses the SIMULINK library of MATLAB, which has proven to be an excellent choice in order to implement and solve the dynamic equations involved. The application allows for the representation of the behavior of a dynamic system analyzed through the Bond Graph theory in order to understand the dynamic equations and the physical phenomena involved. Based on block diagram of SIMULINK, the different "bonds" of Bond Graph can be integrated as SIMULINK blocks in order to generate the dynamic model. A few simple models are analyzed through this application. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 18: 238,251, 2010; Published online in Wiley InterScience (www.interscience.wiley.com); DOI 10.1002/cae.20246 [source] Use of the oligochaete, Lumbriculus variegatus, as a prey organism for toxicant exposure of fish through the dietENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 10 2006David R. Mount Abstract The oligochaete, Lumbriculus variegatus, has several characteristics that make it desirable as a prey organism for conducting dietary exposure studies with fish. We conducted 21- and 30-d experiments with young fathead minnows (Pimephales promelas) and rainbow trout (Oncorhynchus mykiss), respectively, to determine whether a diet consisting solely of L. variegatus would support normal growth and to compare performance with standard diets (Artemia nauplii, frozen brine shrimp, or trout chow). All diets were readily accepted, and fish survived and grew well. Food conversion in both fathead minnows and rainbow trout was as high as or higher for the oligochaete diet compared with others, although this comparison is influenced by differences in ration, ingestion rate, or both. The oligochaete diet had gross nutritional analysis similar to the other diets, and meets fish nutrition guidelines for protein and essential amino acids. Methodologies and practical considerations for successfully using oligochaetes as an experimental diet are discussed. Considering their ready acceptance by fish, their apparent nutritional sufficiency, the ease of culturing large numbers, and the ease with which they can be loaded with exogenous chemicals, we believe that L. variegatus represents an excellent choice of exposure vector for exposing fish to toxicants via the diet. [source] Detection of spring viraemia of carp virus (SVCV) by loop-mediated isothermal amplification (LAMP) in koi carp, Cyprinus carpio LJOURNAL OF FISH DISEASES, Issue 4 2008R B Shivappa Abstract Spring viraemia of carp virus (SVCV) is a rhabdovirus associated with systemic illness and mortality in cyprinids. Several diagnostic tests are available for detection of SVCV. However, most of these tests are time consuming and are not well adapted for field-based diagnostics. In this study, a diagnostic tool for SVCV detection based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been developed. Based on the nucleotide sequence of the glycoprotein (G) gene of SVCV North Carolina (NC) isolate, four sets (each set containing two outer and two inner) of primers were designed. Temperature and time conditions were optimized to 65 °C and 60 min, respectively, for LAMP and RT-LAMP using one primer set. In vitro specificity was evaluated using four different strains of fish rhabdoviruses and RT-LAMP was found to be specific to SVCV. Serial dilutions of SVCV NC isolate was used to evaluate the in vitro sensitivity of RT-LAMP. Sensitivity of the assays was similar to RT-PCR and detected SVCV even at the lowest dilution of 101 TCID50 mL,1. The ability of RT-LAMP to detect SVCV from infected carp was also tested and the assay detected SVCV from all infected fish. The isothermal temperature requirements, high specificity and sensitivity, and short incubation time of the RT-LAMP assay make it an excellent choice as a field diagnostic test for SVCV. [source] Solid-phase peptide synthesis using acetonitrile as a solvent in combination with PEG-based resinsJOURNAL OF PEPTIDE SCIENCE, Issue 10 2009Gerardo A. Acosta Abstract This manuscript shows that ACN can be an excellent choice for the coupling of hindered amino acids as illustrated by the coupling of Fmoc-amino acids on free amino acids anchored on a BAL synthesis. Furthermore, ACN can be a good alternative for solid-phase peptide synthesis in the absence of DMF (washings, removal of Fmoc, and coupling). Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd. [source] A comparison of capillary-scale LC,NMR with alternative techniques: spectroscopic and practical considerations,MAGNETIC RESONANCE IN CHEMISTRY, Issue 9 2005Richard J. Lewis Abstract Experimental and practical details for the use of capillary LC (CapLC),NMR are reported. The capillary NMR probe has high sensitivity and excellent flow characteristics and we found CapLC,NMR to be best suited to samples that are truly mass limited. CapLC,NMR relies on good capillary-scale chromatography where highly concentrated peaks with a volume closely matched to the NMR flow cell are achievable. Provided that the loading capacity of the capillary column is not limiting, the combination of high sensitivity and high solvent suppression quality makes CapLC,NMR an excellent choice. For many real samples, however, the loading is limiting and we found the combination of LC,SPE,MS,NMR with a cryoprobe enables more material to be purified for NMR analysis, while retaining sensitivity. Copyright © 2005 John Wiley & Sons, Ltd. [source] A multigrid upwind strategy for accelerating steady-state computations of waves propagating with curvature-dependent speedsNUMERICAL METHODS FOR PARTIAL DIFFERENTIAL EQUATIONS, Issue 2 2002Jonathan Rochez Abstract A multigrid strategy using upwind finite differencing is developed for accelerating the steady state computations of waves, [14] propagating with curvature-dependent speeds. This will allow the rapid computation of a "burn table." In a high explosive material, a burn table will allow the elimination of solving chemical reaction ODEs by feeding in source terms to the reactive flow equations for solution of the system of ignition of the high explosive material. Standard iterative methods show a quick reduction of the residual followed by a slow final convergence to the solution at high iterations. Such systems, including a nonlinear system such as this, are excellent choices for the use of multigrid methods to speed up convergence. Numerical steady-state solutions to the eikonal equation on several test grids are conducted. Results are presented for these cases in 2D and a cubic grid in 3D using a Runge-Kutta time iteration for the smoothing operator until steady state is reached. © 2002 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq 18: 179,192, 2002; DOI 10.1002/num.1002 [source] | ||||||||||