Agricultural Pests (agricultural + pest)

Distribution by Scientific Domains
Distribution within Life Sciences


Selected Abstracts


Enhancing Biological Control: Habitat Management to Promote Natural Enemies of Agricultural Pests

JOURNAL OF APPLIED ENTOMOLOGY, Issue 4 2002
Article first published online: 9 OCT 200
First page of article [source]


Effect of 13 single and eight mixed host plant diets on survival, post-embryonic development and morphology of variegated grasshopper in laboratory

ENTOMOLOGICAL RESEARCH, Issue 1 2010
Sévilor KEKEUNOU
Abstract Zonocerus variegatus (Orthoptera: Pyrgomorphidae) is known as an agricultural pest in West and Central Africa. In this study, the effects of 13 single and eight mixed host plant diets on the survival, post-embryonic development and morphology of Z. variegatus in the laboratory were investigated. In each cage, 20 one-day larvae were provided with these diets and their survival monitored every two days, from August 2000 to September 2002, until all individuals died. Results showed that the number of larval stages varied from six to eight according to the diet. Six larval stages were most frequent. Development to the adult stage (complete development) was only noted with 52% of diets. All the mixed host plant diets induced complete development of Z. variegatus except that of Cajanus cajan + Synedrella nodiflora + Chromolaena odorata. Complete development was observed with four single host plant diets: Lablab purpurea, C. cajan, Manihot esculenta and S. nodiflora. Compared to leguminous species and Asteraceae, M. esculenta (Euphorbiaceae) was the species that resulted in the highest level of survival and development of Z. variegatus. Chromolaena odorata and S. nodiflora (Asteraceae) induced late appearance of the different Z. variegatus nymphal instars and the appearance time was shorter on legumes. The supernumerary stage 7 has the same color as the larva in stages 1,6, but this larva was significantly larger than stage 6 and smaller than the adult stage. These results indicate that legumes for short fallows affected the survival and post-embryonic development of Z. variegatus in the laboratory. [source]


Effect of the antimicrobial peptide indolicidin on the green peach aphid Myzus persicae (Sulzer)

JOURNAL OF APPLIED ENTOMOLOGY, Issue 2 2007
R. R. Le-Feuvre
Abstract:, The green-peach aphid, Myzus persicae (Sulzer) (Hem., Aphididae), is a major agricultural pest of a wide range of host plants, causing damage by feeding and indirectly by transmitting viruses. In this study we tested the effect of the antimicrobial peptide indolicidin on M. persicae survival and on its essential bacterial endosymbionts. Artificial diet bioassays showed a significant dose-dependent lethal response of indolicidin on M. persicae survival (LD50 of 209 ± 60 ,g/ml). Histological analysis of indolicidin-treated aphids revealed a lower number of distorted mycetocytes, whereas control aphids showed abundant number of rounded and filled mycetocytes. These results suggest that aphid survival could be affected via reduction of its endosymbionts. Thus, aphid control based on antimicrobial substances against endosymbionts could be a promising strategy that needs to be further explored. [source]


Macrogeographic population structuring in the cosmopolitan agricultural pest Bactrocera cucurbitae (Diptera: Tephritidae)

MOLECULAR ECOLOGY, Issue 13 2010
M. VIRGILIO
Abstract The macrogeographic population structure of the agricultural pest Bactrocera cucurbitae (Diptera: Tephritidae) was investigated in order to identify the geographic origin of the species and reconstruct its range expansion. Individuals of B. cucurbitae were collected from 25 worldwide-distributed localities (n = 570) and genotyped at 13 microsatellite loci. The Bayesian clustering reveals that B. cucurbitae can be subdivided into five main groups corresponding to populations from (i) the African continent, (ii) La Réunion, (iii) Central Asia, (iv) East Asia and (v) Hawaii. The proportions of inter-regional assignments and the higher values of genetic diversity in populations from Pakistan, India and Bangladesh suggest that B. cucurbitae originated in Central Asia and expanded its range to East Asia and Hawaii on one hand and to Africa and the islands of the Indian Ocean on the other. A number of outliers (10,19 specimens according to different clustering algorithms) show high levels of admixture (Q > 0.70) with populations from different regions and reveal complex patterns of inter-regional gene flow. Anthropogenic transport is the most plausible promoter of this large-scale dispersal. The introduction of individuals from geographically distant sources did not have a relevant role in the most recent African invasions, which originated from the expansion of local populations. These results could provide a useful background to better evaluate invasion risks and establish priorities for the management of this cosmopolitan agricultural pest. [source]


Use of Olfaction During Prey Location by the Common Vampire Bat (Desmodus rotundus),

BIOTROPICA, Issue 1 2007
Joseph Wm.
ABSTRACT The common vampire bat, Desmodus rotundus, feeds on mammalian blood and is a major agricultural pest in Latin America. One way to prevent bats from biting livestock is to understand how the bats locate the livestock. We confirmed the role of olfaction in foraging with a modified cafeteria trial. Bats significantly favored blood meals associated with scent (fur, feces) over meals lacking these olfactory cues. These results confirm earlier observations on the role of olfaction during prey location by this species, and have potential to facilitate eradication measures without extensive loss of other bat species. RESUMEN El vampiro común, Desmodus rotundus, se alimenta del sangre de los mamíferos y es una de las plagas agrícolas más destructivas en América Latina. Para mejor prevenir los murciélagos de mordiendo ganado, debemos comprender mejor cómo los vampiros localizan ganado. Confirmamos el rol de olfacción en forrajeo con una modificación de una prueba "cafetería". Los murciélagos se favorecen las comidas de sangre asociados con olor de vacas (pelos, fecas) sobre las que no tienen estos olores. Estos resultados se confirman observaciones anteriores sobre el rol de olfacción durante la detección de presas por estas especies, y tienen la posibilidad de facilitar medidas de control de vampiros sin la perdida extensiva de otras especies de murciélagos. [source]


Biodiversity and biocontrol: emergent impacts of a multi-enemy assemblage on pest suppression and crop yield in an agroecosystem

ECOLOGY LETTERS, Issue 9 2003
Bradley J. Cardinale
Abstract The suppression of agricultural pests has often been proposed as an important service of natural enemy diversity, but few experiments have tested this assertion. In this study we present empirical evidence that increasing the richness of a particular guild of natural enemies can reduce the density of a widespread group of herbivorous pests and, in turn, increase the yield of an economically important crop. We performed an experiment in large field enclosures where we manipulated the presence/absence of three of the most important natural enemies (the coccinellid beetle Harmonia axyridis, the damsel bug Nabis sp., and the parasitic wasp Aphidius ervi) of pea aphids (Acyrthosiphon pisum) that feed on alfalfa (Medicago sativa). When all three enemy species were together, the population density of the pea aphid was suppressed more than could be predicted from the summed impact of each enemy species alone. As crop yield was negatively related to pea aphid density, there was a concomitant non-additive increase in the production of alfalfa in enclosures containing the more diverse enemy guild. This trophic cascade appeared to be influenced by an indirect interaction involving a second herbivore inhabiting the system , the cowpea aphid, Aphis craccivora. Data suggest that high relative densities of cowpea aphids inhibited parasitism of pea aphids by the specialist parasitoid, A. ervi. Therefore, when natural enemies were together and densities of cowpea aphids were reduced by generalist predators, parasitism of pea aphids increased. This interaction modification is similar to other types of indirect interactions among enemy species (e.g. predator,predator facilitation) that can enhance the suppression of agricultural pests. Results of our study, and those of others performed in agroecosystems, complement the broader debate over how biodiversity influences ecosystem functioning by specifically focusing on systems that produce goods of immediate relevance to human society. [source]


Juvenile Hormone Binding Protein Titers During Adult Metamorphosis in Manduca sexta

ENTOMOLOGICAL RESEARCH, Issue 4 2003
Young Jin KANG
ABSTRACT ABSTRACT During the pupal and adult stages, the JHBP levels displayed a sex-related difference, with females showing higher levels than males. A sharp increase in JHBP levels was observed at day 2 of the pupal stage. After day 2 the JHBP titer declined precipitously, and then remained unchanged until day 12 in males. JHBP titers in females decreased slightly after day 2 and then remained relatively constant until day 12. In both sexes, the JHBP levels showed a steep increase and peaked around day 15. During the previtellogenic period, the JHBP titers declined dramatically until adult ecdysis. During early adult stage, JHBP titiers in the female remained constant at preecdysis level. This information could broaden our understanding of pest physiology during adult metamorphosis, and could have extensive implications for developing insect growth regulators to control agricultural pests. [source]


Problems of resistance development in arthropod pests of agricultural crops in Russia

EPPO BULLETIN, Issue 1 2008
G. I. Sukhoruchenko
This paper presents the results of long-term monitoring of insecticide resistance in populations of agricultural pests in Russia. Over the last 45 years, resistance developments were recorded for 36 arthropod pest species in 11 agricultural crops and pastures in relation to nearly all commonly used plant protection products. Development of group, cross and multiple resistance has been revealed in populations of many economically important pests. Toxicological and phenotypical (for Colorado potato beetle) methods have been devised to monitor the development of pesticide resistance. Based on experience over the last century, systems aimed at preventing the development of pest resistance to insecticides and acaricides are elaborated. These systems are based on resistance monitoring and using plant protection measures which minimize the toxic pressure on agroecosystems. [source]


Will climate change be beneficial or detrimental to the invasive swede midge in North America?

GLOBAL CHANGE BIOLOGY, Issue 8 2008
Contrasting predictions using climate projections from different general circulation models
Abstract Climate change may dramatically affect the distribution and abundance of organisms. With the world's population size expected to increase significantly during the next 100 years, we need to know how climate change might impact our food production systems. In particular, we need estimates of how future climate might alter the distribution of agricultural pests. We used the climate projections from two general circulation models (GCMs) of global climate, the Canadian Centre for Climate Modelling and Analysis GCM (CGCM2) and the Hadley Centre model (HadCM3), for the A2 and B2 scenarios from the Special Report on Emissions Scenarios in conjunction with a previously published bioclimatic envelope model (BEM) to predict the potential changes in distribution and abundance of the swede midge, Contarinia nasturtii, in North America. The BEM in conjunction with either GCM predicted that C. nasturtii would spread from its current initial invasion in southern Ontario and northwestern New York State into the Canadian prairies, northern Canada, and midwestern United States, but the magnitude of risk depended strongly on the GCM and the scenario used. When the CGCM2 projections were used, the BEM predicted an extensive shift in the location of the midges' climatic envelope through most of Ontario, Quebec, and the maritime and prairie provinces by the 2080s. In the United States, C. nasturtii was predicted to spread to all the Great Lake states, into midwestern states as far south as Colorado, and west into Washington State. When the HadCM3 was applied, southern Ontario, Saskatchewan, and Washington State were not as favourable for C. nasturtii by the 2080s. Indeed, when used with the HadCM3 climate projections, the BEM predicted the virtual disappearance of ,very favourable' regions for C. nasturtii. The CGCM2 projections generally caused the BEM to predict a small increase in the mean number of midge generations throughout the course of the century, whereas, the HadCM3 projections resulted in roughly the same mean number of generations but decreased variance. Predictions of the likely potential of C. nasturtii spatial spread are thus strongly dependent on the source of climate projections. This study illustrates the importance of using multiple GCMs in combination with multiple scenarios when studying the potential for spatial spread of an organism in response to climate change. [source]


Ace2, rather than ace1, is the major acetylcholinesterase in the silkworm, Bombyx mori

INSECT SCIENCE, Issue 4 2009
Hui-Juan Chen
Abstract, Two acetylcholinesterase (ace) genes have been reported in many insect species. In pests such as Helicoverpa assulta and Plutella xylostellas, ace1 gene encodes the predominant synaptic enzyme that is the main target of organophosphorus (OP) and carbamate pesticides. It has been reported that pesticide selection has an impact on the ace gene evolution. The domesticated silkworm, Bombyx mori, also has two ace genes. We studied ace gene expression and enzyme activities in silkworm as this has not faced pesticide selection over the past decades. The expression levels of two ace genes, Bm- ace1 and Bm- ace2, were estimated by quantitative real-time polymerase chain reaction. Bm- ace2 was expressed more highly than Bm- ace1 in all tested samples of different developmental stages or tissues, suggesting ace2, rather than ace1, is the major type of acetylcholinesterase (AChE) in Bombyx mori. This is inconsistent with the aforementioned lepidopterons agricultural pests, partly be due to the widespread use of pesticides that may induce high expression of the ace1 gene in these pests. Besides high expression in the head, Bm- ace1 also expresses highly in the silk glands and Bm- ace2 is abundant in the germline, implying both ace genes may have potential non-hydrolytic roles in development. Furthermore, we found that the mRNA levels of two ace genes and their ratios (ace2/ace1) change day to day in the first and third instars. This challenges the conventional method of estimating enzymatic activity using crude extract as an enzyme solution, as it is a mixture of AChE1 and AChE2. An efficient and simple method for separating different AChEs is necessary for reliable toxicological analyses. [source]


Beneficial links for the control of aphids: the effects of compost applications on predators and prey

JOURNAL OF APPLIED ECOLOGY, Issue 4 2008
James R. Bell
Summary 1Polyphagous predators, such as spiders and beetles, perform a fundamental ecosystem service as regulators of agricultural pests, particularly aphids. They are most effective when they colonize the crop before the pest has reached its exponential growth phase. However, this is also when predators find themselves in a state of near-starvation. 2Predator numbers can be enhanced by applications of different types of organic matter, but the mechanism is not clearly understood. One hypothesis is that compost applied to the field may introduce a new detrital food chain to maintain predators until the pest arrives, but this may also be detrimental to effective pest control, fostering a surplus of alternative prey and causing a switch away from the pest. To elucidate these possible outcomes, we report on the use of within-field compost applications on aphids and their predators, presenting 4 years of field-scale manipulations. 3We found both direct and indirect links between compost, aphids and predators. In years when compost-treated plots had significantly higher numbers of predators, aphids were in significantly lower numbers than in plots without compost. Conversely, when there was a lack of response by predators, aphid numbers showed similar trends in all treatments. 4In all years, alternative prey responded strongly to compost application and did not fluctuate at the level shown by predators, suggesting that these two prey groups were decoupled. Instead, the predicted positive feedback of compost on predators numbers was either weak or absent. 5Synthesis and applications. The effect of compost on aphids clearly requires further practical refinement if it is to provide constant pest suppression, making it difficult to provide specific management recommendations at this stage. In the short term, compost application may not always confer immediate benefits in terms of pest control alone but this must be set against other better known benefits (moisture retention, nutrients). In the long term, experiments measuring the full trophic pathway are needed to unravel the effects of organic matter type, application time and the siting of compost relative to the crop in order to optimise pest suppression potential. [source]


Genetic identification of morphologically cryptic agricultural pests

AGRICULTURAL AND FOREST ENTOMOLOGY, Issue 1 2009
J. S. Ellis
Abstract 1,Wireworms are the polyphagous larvae of click beetles and are well known as agricultural pests. Larvae of the genus Agriotes are internationally recognized as economically important pests of potato. Historically associated with crop damage after conversion of grassland, they are an increasing problem even in all-arable rotations. 2,Current studies of Agriotes ecology and behaviour, and consequently control and management, are seriously hampered by the lack of a means of reliably identifying larvae owing to morphological crypsis during this life-stage. 3,Here, sequence data at the mitochondrial 16SrRNA gene are presented for three species of wireworm: Agriotes obscurus, A. lineatus, and A. sputator. A novel terminal restriction fragment length polymorphism (T-RFLP) technique is described that identifies larvae of these species. This technique is shown to be both efficient and reliable. Interestingly, thus far the samples tested have yielded no A. lineatus. Implications for future study of wireworm ecology and control are discussed. [source]


Pesticides and the intoxication of wild animals

JOURNAL OF VETERINARY PHARMACOLOGY & THERAPEUTICS, Issue 2 2007
P. BERNY
Pesticides are widely used to control agricultural pests. Unfortunately, because of their biological activity, they may have detrimental effects on nontarget species. Acute toxicity, resulting in death, is reported worldwide. Although chronic and indirect effects may be even more hazardous for animal populations on a long-term basis, they are usually evaluated as parts of research programs. The purpose of this paper was to review the wildlife-poisoning surveillance systems and their results regarding the circumstances of exposure of wild animals, the pesticides involved and the species exposed. Most data are retrieved from the British and French pesticide poisoning surveillance systems in wildlife, with some data from other European structures. [source]


Polymorphic microsatellite loci from northern and Mexican corn rootworms (Insecta: Coleoptera: Chrysomelidae) and cross-amplification with other Diabrotica spp.

MOLECULAR ECOLOGY RESOURCES, Issue 3 2008
ERIC R. WAITS
Abstract The northern corn rootworm (Diabrotica barberi) and Mexican corn rootworm (Diabrotica virgifera zeae) are significant agricultural pests. For the northern corn rootworm, and to a lesser extent, the Mexican corn rootworm, high resolution molecular markers are needed. Here we present 14 polymorphic microsatellite loci isolated from libraries constructed using pooled northern and Mexican corn rootworm genomic DNA. Polymorphism in other Diabrotica, including the banded cucumber beetle, southern corn rootworm and western corn rootworm, is described. [source]


The putative-farnesoic acid O -methyl transferase (FAMeT) gene of Ceratitis capitata: characterization and pre-imaginal life expression

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY (ELECTRONIC), Issue 2 2010
Laura Vannini
Abstract Farnesoic acid O -methyl transferase (FAMeT) is the enzyme involved in the penultimate step of insect juvenile hormone (JH) biosynthesis and is thus a key regulator in insect development and reproduction. We report the characterization of the putative- FAMeT in the medfly or Mediterranean fruit fly, Ceratitis capitata. This gene was identified by suppressive subtractive hybridization and completely sequenced by the screening of a medfly cDNA library. The obtained sequence was analyzed for conserved protein domain identification and its expression profile was evaluated by quantitative Real-Time PCR in medfly pre-imaginal life. The tissue expression of the isolated gene was verified by in situ hybridization on third instar larvae sections. The characterization of the isolated gene pointed out several typical features of methyl transferase genes. The pre-imaginal putative- FAMeT expression levels were consistent with JH titer change in Diptera. As recognized in some crustaceans, this gene seems to be widely expressed in the medfly as well. Ceratitis capitata is one of the most relevant agricultural pests against which insecticides and the sterile insect technique (SIT) are extensively used in spite of the well-known limitations of these approaches. Although results are not conclusive for the physiological role of the isolated gene, they suggest the characterization of a new gene in the Mediterranean fruit fly potentially involved in JH biosynthesis and may, therefore, have implications for pest control. © 2010 Wiley Periodicals, Inc. [source]


The piercing-sucking herbivores Lygus hesperus and Nezara viridula induce volatile emissions in plants ,,

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY (ELECTRONIC), Issue 2 2005
Livy Williams III
Abstract Plant volatiles induced by herbivory are often used as olfactory cues by foraging herbivores and their natural enemies, and thus have potential for control of agricultural pests. Compared to chewing insects and mites, little is known about plant volatile production following herbivory by insects with piercing-sucking mouthparts. Here, we studied factors (insect life stage, gender, the role of salivary glands, and type of bioassay used for volatile induction) that influence the induction of plant volatiles by two agriculturally important hemipterans, Lygus hesperus and Nezara viridula. Feeding on intact cotton by virgin females of L. hesperus induced 2.6-fold greater volatile response compared to that induced by mated females, possibly due to increased feeding activity by virgin females. This plant volatile response was associated with elicitors present in the insect's salivary glands as well as to the degree of mechanical injury. Feeding injury by N. viridula females also increased volatile emissions in intact maize by approximately 2-fold compared to control plants. Maize seedlings injured by N. viridula emitted higher amounts of the monoterpene linalool, the sesquiterpenes (E)-,-caryophyllene, ,- trans -bergamotene, and (E,E)-,-farnesene, and the homoterpene (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene, but not amounts of green leaf volatiles, compared to uninjured plants. Emissions from intact maize injured by adult males were lower than those emitted by adult females of the same age and did not differ from those emitted by uninjured plants. Similarly, feeding by virgin female N. viridula followed by excision led to 64% higher quantities of volatiles compared to untreated plants. Volatile emission in excised plants, however, was considerably greater than in intact plants, suggesting that careful consideration must be given to bioassay design in studies of herbivore-induced plant volatiles. Salivary gland extracts of N. viridula led to sesquiterpene emissions approximately 2.5-fold higher than for controls, although no significant differences were observed for green leaf volatiles, monoterpenes, and homoterpenes. These results indicate that L. hesperus and female N. viridula feeding induce volatile production in plants, and that volatile production is affected by gender and life stage of the bug. Although oviposition and mechanical injury by stylets may increase release of volatiles, elicitors from salivary glands of L. hesperus and N. viridula also seem to play a role in the emission of plant volatiles. Arch. Insect Biochem. Physiol. 58:84,96, 2005. Published 2005 Wiley-Liss, Inc. [source]


Ecological role of control agent, and not just host-specificity, determine risks of biological control

AUSTRAL ECOLOGY, Issue 6 2010
COLLEEN L. SEYMOUR
Abstract Biological control agents used to manage alien vegetation are generally viewed as providing an ecosystem service, owing to reduced ecological and economic costs of invasion following their release. In particular, gall-formers are popular as biological control agents because they are host-specific and therefore considered low risk. However, galls can also be considered to be ecological engineers, because they provide nutritional resources for native invertebrates. We tested whether native invertebrates had formed associations with the gall-forming fungus Uromycladium tepperianum, introduced into South Africa to control the Australian invasive alien tree Acacia saligna, by collecting U. tepperianum galls and monitoring emergence. We found that a number of invertebrates had formed associations with the biological control agent, among which was the important citrus pest, Thaumatotibia leucotreta (false codling moth). We used pheromone-baited traps to ascertain if this supplementary source of T. leucotreta increased their abundance in orchards close to patches of gall host, but did not find this to be the case. We did find, however, that control measures used by farmers explained T. leucotreta abundances in traps, which may have obscured detection of any effects of a nearby host for the pest. Nevertheless, this study illustrates the first case of a host-specific classical biological control agent providing resources for an economically significant crop pest. We conclude that although biological control agents are strictly vetted to ensure host-specificity, introduced biological control agents that become abundant and can act as ecological engineers pose risks when native biota form associations with them, resulting in a number of possible cascading ecosystem effects. In addition, there could be economic consequences when these associated species include agricultural pests. We conclude that not just host specificity, but potential ecological effects of biological control agents, should be considered in their selection. [source]


Arthropod prey of shelterbelt-associated birds: linking faecal samples with biological control of agricultural pests

AUSTRALIAN JOURNAL OF ENTOMOLOGY, Issue 4 2007
Sagrario Gámez-Virués
Abstract, The value of insectivorous birds as agents for biological control of arthropod pests has been little studied, especially in Australia. This paper reports on the extent to which arthropods from various pest and non-pest taxa feature in the diets of birds captured in farm shelterbelts in central western New South Wales. The parameters examined were the types of arthropod fragments in bird faeces and percentage volume and frequency of occurrence of each component. The faecal data were compared with samples of the arthropod fauna trapped in shelterbelts during the period the birds were captured. In 26 of 29 faecal samples, arthropod fragments were the predominant components, the most common being from Coleoptera, Hymenoptera (especially Formicidae), Orthoptera and Araneae. The recognisable pest taxa in faecal samples were Scarabaeidae and wingless grasshopper Phaulacridium vittatum (Sjöstedt) (Orthoptera: Acrididae). The results indicate that the native bird species common in farm shelterbelts preyed on a range of arthropod taxa including several that are pests of crops and pastures. Accordingly, conservation of birds in farmlands could contribute to suppression of arthropod pests. [source]


Mity model: Tetranychus urticae, a candidate for chelicerate model organism

BIOESSAYS, Issue 5 2007
Miodrag Grbic
Chelicerates (scorpions, horseshoe crabs, spiders, mites and ticks) are the second largest group of arthropods and are of immense importance for fundamental and applied science. They occupy a basal phylogenetic position within the phylum Arthropoda, and are of crucial significance for understanding the evolution of various arthropod lineages. Chelicerates are vectors of human diseases, such as ticks, and major agricultural pests, such as spider mites, thus this group is also of importance for both medicine and agriculture. The developmental genetics of chelicerates is poorly understood and a challenge for the future progress for many aspects of chelicerate biology is the development of a model organism for this group. Toward this end, we are developing a chelicerate genetic model: the two-spotted spider mite Tetranychus urticae. T. urticae has the smallest genome of any arthropod determined so far (75 Mbp, 60% of the size of the Drosophila genome), undergoes rapid development and is easy to maintain in the laboratory. These features make T. urticae a promising reference organism for the economically important, poorly studied and species-rich chelicerate lineage. BioEssays 29:489,496, 2007. © 2007 Wiley Periodicals, Inc. [source]