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Essential Element (essential + element)
Selected AbstractsUnderstanding Surge Capacity: Essential ElementsACADEMIC EMERGENCY MEDICINE, Issue 11 2006Donna F. Barbisch RN As economic forces have reduced immediately available resources, the need to surge to meet patient care needs that exceed expectations has become an increasing challenge to the health care community. The potential patient care needs projected by pandemic influenza and bioterrorism catapulted medical surge to a critical capability in the list of national priorities, making it front-page news. Proposals to improve surge capacity are abundant; however, surge capacity is poorly defined and there is little evidence-based comprehensive planning. There are no validated measures of effectiveness to assess the efficacy of interventions. Before implementing programs and processes to manage surge capacity, it is imperative to validate assumptions and define the underlying components of surge. The functional components of health care and what is needed to rapidly increase capacity must be identified by all involved. Appropriate resources must be put into place to support planning factors. Using well-grounded scientific principles, the health care community can develop comprehensive programs to prioritize activities and link the necessary resources. Building seamless surge capacity will minimize loss and optimize outcomes regardless of the degree to which patient care needs exceed capability. [source] Promoting Physical Activity in GirlsJOURNAL OF SCHOOL HEALTH, Issue 2 2005A Case Study of One School's Success ABSTRACT: This case study profiles one of 24 high schools that participated in a school-based, NIH-funded study to increase physical activity among high school girls. The case study school was one of 12 randomly assigned to the intervention group. The study intervention was based on the premise that a successful intervention is developed and tailored by teachers and staff to fit the context of their school. Intervention guidelines (Essential Elements) and the Coordinated School Health Program (CSHP) model were used to direct intervention activities for physical education, health education, school environment, school health services, faculty/staff health promotion, and family/community involvement. All girls at the case study school received the intervention. A team of school employees provided leadership to develop and implement the intervention in collaboration with a university project staff. Data collected over a two-year period were used to describe changes that occurred in each CSHP area. Key changes were made in the school environment, curricula, policies, and practices. Qualitative measures showed girls more involved in physical activity. Quantitative measures taken in eighth grade, and repeated with the same set of girls in ninth grade, showed increases in both moderate-to-vigorous physical activity (p = < .01) and vigorous physical activity (p = .04). Other schools can use this case to modify components of the CSHP model to increase physical activity among high school girls. [source] Efficacy of urinary guidelines in the management of post-stroke incontinenceINTERNATIONAL JOURNAL OF UROLOGICAL NURSING, Issue 1 2009Stephanie Vaughn Abstract Urinary incontinence (UI) is common occurrence among stroke survivors and impacts their recovery. This mixed method study examined the effects of implementation of evidence-based urinary guidelines by the Interdisciplinary (ID) team in the management of post-stroke UI in stroke survivors in an acute rehabilitation hospital in Southern California. Essential elements of the guidelines included assessment of the bladder pattern, the urinary WBC's, the implementation of a scheduled toileting program, pelvic floor exercises, and the administration of Vitamin C 500 mg. by mouth. Functional Independent Measure (FIM) scores and urinary white blood cells (WBC's) were used to evaluate the efficacy the guidelines. Post guideline implementation FIM scores and urinary WBC's demonstrated improvement over the pre-scores. These results indicate that positive stroke outcomes were achieved following implementation. In addition, the ID team, comprised of nurses, physical therapists, speech pathologists, and occupational therapists, was queried as to the member's knowledge and perceptions of their roles in the implementation of the guidelines. Highlighted themes from the ID focus groups were communication and structure, relating that the guidelines were useful in promoting collaborative practice among the ID team members. [source] Essential elements and contaminants in tissues of commercial pelagic fish from the Eastern Mediterranean SeaJOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, Issue 9 2009Beyza Ersoy Abstract BACKGROUND: It is important to determine the concentrations of essential and non-essential metals in fish for human health. The essential elements and contaminants (Pb and Cd) were determined seasonally in the muscle and liver of some pelagic fish species round herring (Etrumeus teres), chub mackerel (Scomber japonicus), golden grey mullet (Liza aurata) and Mediterranean horse mackerel (Trachurus mediterraneus) from the Iskenderun Bay, Eastern Mediterranean Sea. RESULTS: The Na, K, Ca and Mg were the most abundant elements in muscle and liver tissues. The Na, K, Ca and Mg concentrations in fish tissues were between 51.7 and 3426 mg kg,1. Muscle accumulated the lowest levels of elements. Trace element and contaminant levels in muscle were highest in spring and summer. The Cu, Zn and Cr concentrations were highest in summer. The Ni, Mn and Fe concentrations were highest in spring. The maximum Pb concentrations in the muscle and liver of fish species was 0.39 and 0.80 mg kg,1 in autumn. The maximum Cd concentration in the muscle of fish was 0.27 mg kg,1 in spring and the maximum Cd concentration in the liver was 0.78 mg kg,1 in summer. CONCLUSION: The Cr, Pb, Cd, Cu and Zn levels in muscle were found to be lower than permissible limits reported by various authorities. Estimated weekly and daily intake for Pb and Cd by consumption of fish muscle were far below the PTWI and PTDI values established by FAO/WHO. Copyright © 2009 Society of Chemical Industry [source] Occlusive Dressing versus Oxygen Mist Therapy Following CO2 Laser ResurfacingDERMATOLOGIC SURGERY, Issue 6 2000Teri Onouye BA Background. Oxygen is an essential element for collagen synthesis and reepithelialization. The use of topical oxygen after CO2 laser resurfacing has not been studied. Objective. To compare the rate and quality of healing in wounds treated with an oxygen mist to those treated with occlusive dressing following CO2 laser resurfacing. Methods. Three patients underwent CO2 laser resurfacing to each half of the face 3 weeks apart. Postoperatively, half of the face was treated with an oxygen mist protocol for 5 days, while the other half was treated with occlusive dressing for 4 days. Results. At postoperative day 5, significantly less crusting was observed on the half of the face treated with the oxygen mist protocol (p < 0.05). Conclusion. The oxygen mist postoperative protocol may offer patients similar overall healing rates and significantly less crusting compared to occlusive dressing. [source] Jumping off Arnstein's ladder: social learning as a new policy paradigm for climate change adaptationENVIRONMENTAL POLICY AND GOVERNANCE, Issue 6 2009Kevin Collins Abstract Participation of citizens, groups, organizations and businesses is now an essential element to tackle climate change effectively at international, European Union, national and local levels. However, beyond the general imperative to participate, major policy bodies offer little guidance on what this entails. We suggest that the dominance of Arnstein's ladder of citizen participation in policy discourses constrains the ways we think about, and critically the purposes we ascribe to, participation in a climate change context. We suggest an alternative framing of climate change, where no single group has clear access to understanding the issue and its resolution. Thus adaptation is fundamentally dependent on new forms of learning. Drawing on experiences of social learning approaches to natural resource managing, we explore how a commitment to social learning more accurately embodies the new kinds of role, relationship, practice and sense of purpose required to progress adaptive climate change agendas and practices. Copyright © 2009 John Wiley & Sons, Ltd and ERP Environment. [source] Predictive Clinical Factors for the Differential Diagnosis of Childhood Extratemporal SeizuresEPILEPSIA, Issue 8 2005András Fogarasi Summary:,Purpose: To describe predictive clinical factors for the differentiation between childhood frontal lobe epilepsy (FLE) and posterior cortex epilepsy (PCE). Methods: Two independent, blinded investigators analyzed 177 seizures from 35 children (aged 11 months to 12 years) with extratemporal epilepsy selected by postoperative seizure-free outcome. Semiologic seizure components and different periictal signs were observed. Age at onset, auras, seizure frequency, and nocturnal dominance, as well as surgical and histopathologic data, were collected from medical charts. Results: Twenty patients had FLE, and 15 had PCE. Patients from both groups had daily seizures without significant differences in frequency but with higher nocturnal dominance in children with FLE (p < 0.05). Visual aura, nystagmus, and versive seizure were observed exclusively in the PCE group, whereas somatosensory aura and hypermotor seizures appeared only in FLE. Tonic seizures were significantly more frequent in FLE (p < 0.01), whereas the presence of clonic seizure (FLE; p = 0.07) and postictal nose-wiping (PCE; p = 0.05) showed only a trend to localize the seizure-onset zone. Myoclonic seizures, epileptic spasms, psychomotor seizures, atonic seizures, oral and manual automatisms, as well as vocalization and eye deviation appeared in both groups without significant differences in their frequency. Conclusions: Characteristic features described in adults' extratemporal epilepsies were frequently missing during childhood seizures, especially in infants and preschool children. Ictal features help only a little in differentiating childhood FLE from PCE. Nocturnal appearance and the type of aura have high localizing value; therefore an accurate history taking is still an essential element of pediatric presurgical evaluation. [source] Communications skills in dental education: a systematic research reviewEUROPEAN JOURNAL OF DENTAL EDUCATION, Issue 2 2010J. A. Carey Abstract Communication is an essential element of the relationship between patient and dentist. Dental schools are required to ensure that undergraduates are adequately trained in communication skills yet little evidence exists to suggest what constitutes appropriate training and how competency can be assessed. This review aimed to explore the scope and quality of evidence relating to communication skills training for dental students. Eleven papers fitted the inclusion criteria. The review found extensive use amongst studies of didactic learning and clinical role-play using simulated patients. Reported assessment methods focus mainly on observer evaluation of student interactions at consultation. Patient involvement in training appears to be minimal. This review recommends that several areas of methodology be addressed in future studies, the scope of research extended to include intra-operative communication, and that the role of real patients in the development of communication skills be active rather than passive. [source] Craving: what can be done to bring the insights of neuroscience, behavioral science and clinical science into synchronyADDICTION, Issue 8s2 2000Roger E. Meyer Alcohol self-administration behavior is the common thread that is necessary to bring the insights of neuroscience, behavioral science and clinical science into synchrony around the concept of craving. Animal models should address the molecular and cellular changes that take place in behaviorally relevant brain regions of rats consequent to chronic self-administration of ethanol. Animal models can focus on the biology of the anticipatory state in alcohol preferring/consuming rats, as well as studies of the effects of possible medications on this state in the animal model, on actual alcohol consuming behavior, and on the residual effects of chronic alcohol on the non-human mammalian brain. In human studies of craving, cue-reactivity in the absence of the opportunity to drink alcohol does not have the same salience as cue-reactivity in which drinking is possible. Moreover, actual drinking behavior serves to validate self-reports of craving. Studies of limited alcohol self-administration in the laboratory are an essential element in screening new medications for the treatment of alcoholism. Studies to date suggest no adverse reaction to the participation of alcoholic subjects in limited alcohol self-administration studies, but the research community should continue to monitor carefully the outcomes of alcohol-dependent subjects who participate in this type of research, and efforts should always be made to encourage these subjects to enter active treatment. In outpatient clinical trials of new treatments for alcoholism, the assessment of craving should include queries regarding symptoms and signs of protracted abstinence such as sleep disturbances, as well as questions regarding situational craving. Field observations of alcoholics in their favorite drinking environments would contribute greatly to our understanding of the real-world phenomenology of craving. [source] Cross-species divergence of the major recognition pathways of ubiquitylated substrates for ubiquitin/26S proteasome-mediated proteolysisFEBS JOURNAL, Issue 3 2010Antony S. Fatimababy The recognition of ubiquitylated substrates is an essential element of ubiquitin/26S proteasome-mediated proteolysis (UPP), which is mediated directly by the proteasome subunit RPN10 and/or RPN13, or indirectly by ubiquitin receptors containing ubiquitin-like and ubiquitin-associated domains. By pull-down and mutagenesis assays, we detected cross-species divergence of the major recognition pathways. RPN10 plays a major role in direct recognition in Arabidopsis and yeast based on the strong affinity for the long and K48-linked ubiquitin chains. In contrast, both the RPN10 and RPN13 homologs play major roles in humans. For indirect recognition, the RAD23 and DSK2 homologs (except for the human DSK2 homolog) are major receptors. The human RAD23 homolog is targeted to the 26S proteasome by the RPN10 and RPN13 homologs. In comparison, Arabidopsis uses UIM1 and UIM3 of RPN10 to bind DSK2 and RAD23, respectively. Yeast uses UIM in RPN10 and LRR in RPN1. Overall, multiple proteasome subunits are responsible for the direct and/or indirect recognition of ubiquitylated substrates in yeast and humans. In contrast, a single proteasome subunit, RPN10, is critical for both the direct and indirect recognition pathways in Arabidopsis. In agreement with these results, the accumulation of ubiquitylated substrates and severe pleiotropic phenotypes of vegetative and reproductive growth are associated with the loss of RPN10 function in an Arabidopsis T-DNA insertion mutant. This implies that the targeting and proteolysis of the critical regulators involved are affected. These results support a cross-species mechanistic and functional divergence of the major recognition pathways for ubiquitylated substrates of UPP. Structured digital abstract ,,A list of the large number of protein-protein interactions described in this article is available via the MINT article ID MINT-7307429 [source] The Interdependence of U.S. Troop Deployments and Trade in the Developing WorldFOREIGN POLICY ANALYSIS, Issue 3 2009Glen Biglaiser The relationship between political conflict and trade has contributed to a riveting discussion in international relations about whether trade produces conflict, or whether conflict itself reduces trade. Most studies proxy "the flag" using militarized interstate disputes (MIDs). However, extensions of "the flag" might well obtain in environments short of MIDs. A more general way to proxy the flag is troop deployments. The deployment of military troops is an essential element of foreign policy. Using panel data for 126 developing countries from 1965 to 2002 and a two-stage least square approach, this essay investigates the relationship between trade and United States troop deployments. We find that trade and troops have a nonrecursive relationship: trade follows the flag and troops follow trade. Given the increased insecurity in the world today, the results are timely and reinforce previous research about the reciprocal relationship between the flag and trade. [source] Gap Inc. sees supplier ownership of compliance with workplace standards as an essential element of socially responsible sourcingGLOBAL BUSINESS AND ORGANIZATIONAL EXCELLENCE, Issue 1 2005Dan Henkle The author looks at the evolution of Gap Inc.'s program for socially responsible sourcing,from a policing model to multistakeholder partnerships,and examines the next stage, which includes a promising resource for placing accountability and compliance management systems where they will have the greatest impact,with the factory owners. © 2005 Wiley Periodicals, Inc. [source] 1. NARRATIVE FORM AND HISTORICAL SENSATION: ON SAUL FRIEDLÄNDER'S THE YEARS OF EXTERMINATION,HISTORY AND THEORY, Issue 3 2009ALON CONFINO ABSTRACT Saul Friedländer's magnum opus, The Years of Extermination, has been received worldwide as an exemplary work of history. Yet it was written by a historian who in the last two decades has strenuously asserted the limits of Holocaust representation. At the center of this essay is a problem of historical writing: how to write a historical narrative of the Holocaust that both offers explanations of the unfolding events and also suggests that the most powerful sensation about those events, at the time and since, is that they are beyond words. I explore Friedländer's crafting of such a narrative by considering, first, the role of his attempt in The Years of Extermination to explain the Holocaust and, second, the narrative form of the book. The book is best seen, I argue, not primarily as a work of explanation but as a vast narrative that places an explanation of the Holocaust within a specific form of describing that goes beyond the boundaries of the historical discipline as it is usually practiced. This form of describing goes beyond the almost positivist attachment to facts that dominates current Holocaust historiography. By using Jewish individual testimonies that are interspersed in the chronological history of the extermination, Friedländer creates a narrative based on ruptures and breaks, devices we associate with works of fiction, and that historians do not usually use. The result is an arresting narrative, which I interpret by using Johan Huizinga's notion of historical sensation. Friedländer sees this narrative form as specific to the Holocaust. I view this commingling of irreducible reality and the possibility of art as a required sensibility that belongs to all historical understanding. And in this respect, The Years of Extermination only lays bare more clearly in the case of the Holocaust what is an essential element in all historical reconstruction. [source] Appropriate vertical discretization of Richards' equation for two-dimensional watershed-scale modellingHYDROLOGICAL PROCESSES, Issue 1 2004Charles W. Downer Abstract A number of watershed-scale hydrological models include Richards' equation (RE) solutions, but the literature is sparse on information as to the appropriate application of RE at the watershed scale. In most published applications of RE in distributed watershed-scale hydrological modelling, coarse vertical resolutions are used to decrease the computational burden. Compared to point- or field-scale studies, application at the watershed scale is complicated by diverse runoff production mechanisms, groundwater effects on runoff production, runon phenomena and heterogeneous watershed characteristics. An essential element of the numerical solution of RE is that the solution converges as the spatial resolution increases. Spatial convergence studies can be used to identify the proper resolution that accurately describes the solution with maximum computational efficiency, when using physically realistic parameter values. In this study, spatial convergence studies are conducted using the two-dimensional, distributed-parameter, gridded surface subsurface hydrological analysis (GSSHA) model, which solves RE to simulate vadose zone fluxes. Tests to determine if the required discretization is strongly a function of dominant runoff production mechanism are conducted using data from two very different watersheds, the Hortonian Goodwin Creek Experimental Watershed and the non-Hortonian Muddy Brook watershed. Total infiltration, stream flow and evapotranspiration for the entire simulation period are used to compute comparison statistics. The influences of upper and lower boundary conditions on the solution accuracy are also explored. Results indicate that to simulate hydrological fluxes accurately at both watersheds small vertical cell sizes, of the order of 1 cm, are required near the soil surface, but not throughout the soil column. The appropriate choice of approximations for calculating the near soil-surface unsaturated hydraulic conductivity can yield modest increases in the required cell size. Results for both watersheds are quite similar, even though the soils and runoff production mechanisms differ greatly between the two catchments. Copyright © 2003 John Wiley & Sons, Ltd. [source] Retaining the visitor, enhancing the experience: identifying attributes of choice in repeat museum visitationINTERNATIONAL JOURNAL OF NONPROFIT & VOLUNTARY SECTOR MARKETING, Issue 1 2009Christine Burton How people make choices in relation to cultural and leisure consumption has been explored from the viewpoint of motivation, lifestyle segmentation, and lifecycle. Little is known about the specific characteristics associated with choices to visit, re-visit, or not to visit a museum. Understanding characteristics of choice, developing incentives, bundled packages, and levels of pricing is an essential element in marketing strategies for museums operating in a competitive leisure marketplace. However, determining what really matters to cultural consumers is complex and methodologies to assist in unraveling such complexities are not easily identified. This study aimed to address ways in which people respond to specific incentives as influences in choosing museum visitation. The study was conducted in two major museums in Australia to determine how useful choice modeling is in identifying features that matter to cultural consumers. The results suggest that choice modeling has much to offer in relation to understanding the benefits people are seeking from a museum experience as well as offering strategic insight into potential collaborative ventures and re-combinations of existing museum products and services. Copyright © 2008 John Wiley & Sons, Ltd. [source] LDL lipid apheresis rapidly increases peripheral endothelial progenitor cell competenceJOURNAL OF CLINICAL APHERESIS, Issue 5 2009Daniel Patschan Abstract Background and Aim: Endothelial progenitor cells (EPCs) have been shown to promote neovascularization under physiologic and pathologic conditions. Statins have been documented to increase the total number of circulating EPCs in long-term treated patients. Lipid apheresis is used to treat patient with refractory hyperlipidemia. The aim of our study was to evaluate whether lipid apheresis is associated with EPC mobilization. Methods: Thirteen patients with refractory hyperlipidemia (analysis at the beginning and at the end of a single lipid apheresis treatment) and 10 healthy controls were included into the study. For quantifying total peripheral EPCs, CD133+/Flk-1+ myelo-monocytic blood cells were enumerated by flow cytometry. The proliferative potential of EPCs was evaluated by a "colony-forming unit" assay. In some patients, EPC eNOS expression was evaluated before and after treatment. Results: Circulating EPCs and the cells' proliferative activity were lower in hyperlipidemia patients as compared to controls (0.14 ± 0.07 vs. 0.6 ± 0.14, P = 0.01, and 13.9 ± 4.9 vs. 45.6 ± 8.1, P = 0.0007). Lipid apheresis treatment was not associated with an increase in total EPCs. The cells' proliferative activity was strongly stimulated by lipid apheresis as reflected by an increase in the number of EPC colonies (13.9 ± 4.9 to 34.1 ± 7.3, P = 0.035). Analysis of EPC eNOS expression revealed a threefold increase in the cellular expression intensity after lipid apheresis. Conclusions: Patients with refractory hyperlipidemia exhibit lower peripheral EPC numbers and a lower proliferative activity of circulating EPCs than healthy controls. A single lipid apheresis treatment significantly stimulates EPC proliferation, it furthermore increases cellular eNOS. In summary, these results show that lipid apheresis mediates beneficial effects on the EPC system as an essential element in the process of vascular repair in the human organism. J. Clin. Apheresis 2009. © 2009 Wiley-Liss, Inc. [source] Undergraduate nursing students attitude to mental health nursing: a cluster analysis approachJOURNAL OF CLINICAL NURSING, Issue 22 2009Karla Gough Aims., The use of cluster analysis to determine if specific groups of students could be identified based on their attitudes towards mental health nursing following the completion of a clinical experience in a mental health setting. Background., Research suggests that nursing students generally have a negative image of mental health nursing. This can be improved following clinical exposure in mental health settings, however, specific aspects of clinical experience that might facilitate attitudinal change have been under-researched. Design., Survey. Methods., A survey was administered to students (n = 703) immediately after completion of their clinical experience. Cluster analysis was used to identify natural groupings within the study cohort. Results., Three distinct clusters were identified. Cluster 1 demonstrated more positive attitudes, greater confidence and viewed mental health more positively than students in the other two Clusters. They were more likely to be male, have spent at least 30 minutes per shift with a preceptor and have completed shifts of eight hours rather than seven hours. Conclusions., Attitudes to mental health nursing may be influenced by specific demographic characteristics of students and by specific aspects of their clinical experience. Relevance to clinical practice., The nursing workforce is an essential element of quality mental health service delivery. Knowledge about factors influencing more positive attitudes is important for structuring clinical experience and designing effective recruitment strategies to attract more students into this field of practice. [source] Toothbrushing promotes gingival fibroblast proliferation more effectively than removal of dental plaqueJOURNAL OF CLINICAL PERIODONTOLOGY, Issue 9 2002Masazumi Horiuchi Abstract Objectives: Removal of dental plaque is an essential element of periodontal treatment. However, there have also been studies of the effects of the mechanical stimulation provided by toothbrushing on gingival host-defense mechanisms. The aim of the study was to evaluate the effects of toothbrushing on gingival fibroblast proliferation in dogs over time, compared to effects of plaque removal without brushing. Methods: The mouths of six mongrel dogs were divided into four quadrants: two for daily toothbrushing, and two for daily plaque removal with a curette. After 1, 3 and 5 weeks of treatment, histometrical analyses were performed to assess inflammatory cell infiltration, proliferating cell nuclear antigen (PCNA)-positive fibroblasts, procollagen type I-positive fibroblasts in the subepithelial connective tissue of junctional epithelium. Results: Toothbrushing increased the number of PCNA-positive fibroblasts in the first week, increased the number of type I procollagen-positive fibroblasts at the fifth week, and reduced inflammatory cell infiltration at the third week. Conclusion: These findings suggest that mechanically stimulated fibroblasts begin proliferating within a week, and this cell division results in an increased number of fibroblasts at the third week. It takes 5 weeks before differences in collagen synthesis between brushing and plaque removal areas are detectable. Zusammenfassung Die Proliferation der gingivalen Fibroblasten wird durch Zähneputzen wirkungsvoller gefördert als durch Plaqueentfernung Ziele: Die Entfernung von Zahnplaque ist ein essenzieller Bestandteil der Parodontalbehandlung. Es gibt jedoch auch Studien über die Wirkung einer durch Zähneputzen bewirkten mechanischen Stimulation der gingivalen Abwehrmechanismen. Ziel dieser Studie war es, bei Hunden die Wirkung des Zähneputzen auf die Proliferation der gingivalen Fibroblasten über eine gewisse Zeit zu untersuchen und mit der Wirkung einer Plaqueentfernung ohne Zähneputzen zu vergleichen. Methoden: Das Maul von 6 Mischlingshunden wurde in vier Quadranten unterteilt: zwei mit täglichem Zähneputzen und zwei mit täglicher Plaqueentfernung mittels Kürette. 1, 3 und 5 Wochen nach der Behandlung wurden histometrische Analysen durchgeführt um das entzündliche Zellinfiltrat, die proliferierenden Cell-Nuclear-Antigen (PCNA)-positiven Fibroblasten und die Prokollagen-I-positiven Fibroblasten des subgingivalen Bindegewebes des Saumepithels zu bestimmen. Ergebnisse: Zähneputzen erhöhte in der ersten Woche die Anzahl der PCNA-positiven Fibroblasten, erhöhte bis zur fünften Woche die Anzahl der Type-I-Prokollagen-positiven Fibroblasten und reduzierte das entzündliche Zellinfiltrat bis zur dritten Woche. Schlussfolgerung: Diese Ergebnisse lassen annehmen, dass mechanisch stimulierte Fibroblasten während einer Woche zu proliferieren beginnen und diese Zellteilung eine erhöhte Anzahl von Fibroblasten in der dritten Woche zum Ergebnis hat. Es dauert fünf Wochen bevor zwischen den Bereichen mit Zähneputzen und Plaqueentfernung Unterschiede in der Kollagensynthese nachweisbar sind. Résumé Le brossage dentaire favorise la prolifération des fibroblastes gingivaux d'une manière plus efficace que l'enlèvement de la plaque dentaire L'enlèvement de la plaque dentaire est un élément essentiel dans le traitement parodontal. Cependant, des études ont été menées sur les effets de la stimulation mécanique produit par le brossage dentaire sur les mécanismes de défense de l'hôte au niveau gingival. Le but de l'étude présente a été d'évaluer les effets du brossage dentaire sur la prolifération des fibroblastes gingivaux chez les chiens dans le temps, comparés aux effets de l'enlèvement de la plaque dentaire sans brossage. Les bouches de six chiens bâtards ont été divisés en quatre quadrants : deux pour un brossage dentaire journalier et deux pour l'enlèvement journalier de la plaque à l'aide d'une curette. Après une, trois et cinq semaines de traitement, les analyses histométriques ont été effectuées pour évaluer l'infiltration cellulaire inflammatoire, les fibroblastes positifs à l'antigène du noyau cellulaire proliférant (PCNA), les fibroblastes positifs au procollagène-I dans le tissu conjonctif sous-épithélial de l'épithélium de jonction. Le brossage dentaire augmentait le nombre de fibroblastes positifs (PCNA) durant la première semaine, augmentait le nombre de fibroblastes positifs au collagène type-1 à la cinquième semaine et réduisait l'infiltration cellulaire inflammatoire à la troisième semaine. Ces découvertes suggèrent que les fibroblastes stimulés mécaniquement commencent à proliférer en une semaine, et cette division cellulaire abouti en un nombre plus important de fibroblastes à la troisième semaine. Il faut attendre cinq semaines avant que des différences dans la synthèse du collagène entre les zones de brossage et d'enlèvement de la plaque dentaire ne soient détectables. [source] Learners' evaluation of a navigation support tool in distance educationJOURNAL OF COMPUTER ASSISTED LEARNING, Issue 5 2007C. Bolman Abstract This article investigates the usability of a navigation support tool, which guides learners by generating advice on the next best step to take in a self-study e-learning course. The article draws on log data and responses from online questionnaires to provide insights into learners' evaluation of the tool, their adherence to the advice and their expectations of self-efficacy. The theoretical underpinnings of the work are described together with the experimental set-up. Results show that more than half of the learners in the experimental group adhered to the advice and held the opinion that the advice stimulated them to proceed with the course. Learners expressed a need to know what the advice was based on which can be seen as an essential element in future development of the tool. [source] Altered signalling from germline to intestine pushes daf-2;pept-1 Caenorhabditis elegans into extreme longevityAGING CELL, Issue 4 2010Britta Spanier Summary The insulin-like signalling pathway is a central regulator of development, metabolism, stress resistance and lifespan in eukaryotes. Caenorhabditis elegans daf-2(e1370) animals with a loss-of-function mutation in the insulin-like receptor live twice as long as wild-type animals, and the additional knockout of the intestinal di- and tripeptide transporter pept-1 further increases lifespan by 60%. In assessing the underlying molecular mechanisms for this phenomenon, microarray-based transcriptome data sets of daf-2(e1370) and daf-2(e1370);pept-1(lg601) animals were compared with a focus on genes that showed significantly higher changes in expression levels in daf-2;pept-1 than in daf-2. We identified 187 genes with at least fourfold decreased transcript levels and 170 with more than a fourfold increase. A large fraction of the down-regulated genes encode proteins involved in germline proliferation and reproduction. The DAF-9/DAF-12 signalling cascade was identified as a prime pathway that mediates the longevity of daf-2;pept-1 with a strict dependance on DAF-16. Loss of DAF-9/DAF-12 or KRI-1 reduces the lifespan of daf-2;pept-1 to that of the daf-2 mutant. Amongst the DAF-16 target genes, numerous enzymes involved in the defence of reactive oxygen species were with increased expression level in daf-2;pept-1. On a functional level, it was demonstrated that amongst those, a high de novo synthesis rate of glutathione is most important for the longevity phenotype of this strain. Taken together, a close interdependence of endocrine hormone signalling from germline to intestine was identified as an essential element in the control of the extreme longevity of C. elegans lacking a proper function of the insulin receptor and lacking the intestinal peptide transporter. [source] In vitro Transient Expression System of Latex C-serum was used for Analysis of Hevein Promoter in Response to Abscisic Acid in Hevea brasiliensisJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 3 2008Xiao-Wen Fei Abstract Hevein has been found to be an essential element in coagulation of rubber particles in latex of rubber trees. In a previous study, we cloned a 1 241-bp fragment of a 5, upstream region of the hevein gene by genome walking. This fragment was analyzed by a 5, end nested deletion method in the present study, fused with a uidA (gus) gene to produce a series of tested constructs, which were transferred into C-serum of latex and the Gus activities were detected. Results showed that the fragment from ,749 to ,292 was sufficient for expression of gus gene in latex, and the fragment from ,292 to ,168 was crucial in response to abscisic acid inducement. In a transient transgenic test of rubber leaf with particle bombardment, construct Hev749 conferred gus -specific expression in veins, in which the latex tubes mainly distributed. This implies that the fragment from ,749 to ,292 was laticiferous-specific. [source] Rice Mitochondrial Genes Are Transcribed by Multiple Promoters That Are Highly DivergedJOURNAL OF INTEGRATIVE PLANT BIOLOGY, Issue 12 2006Qun-Yu Zhang Abstract Plant mitochondrial genes are often transcribed into complex sets of mRNA. To characterize the transcription initiation and promoter structure, the transcript termini of four mitochondrial genes, atp1, atp6, cob, rps7, in rice (Oryza sativa L.), were determined by using a modified circularized RNA reverse transcription-polymerase chain reaction method. The results revealed that three genes (atp1, atp6, rps7) were transcribed from multiple initiation sites, indicating the presence of multiple promoters. Two transcription termination sites were detected in three genes (atp6, cob, rps7), respectively. Analysis on the promoter architecture showed that the YRTA (Y=T or C, R=A or G) motifs that are widely present in the mitochondrial promoters of other monocotand dicot plant species were detected only in two of the 12 analyzed promoters. Our data suggest that the promoter sequences in the rice mitochondrial genome are highly diverged in comparison to those in other plants, and the YRTA motif is not an essential element for the promoter activity. (Managing editor: Li-Hui Zhao) [source] A new heat balance for flow boilingAICHE JOURNAL, Issue 8 2007Francisco J. Collado Abstract Recently, one of the authors suggested calculating void fraction, an essential element in thermal-hydraulics, working with the "thermodynamic" quality instead of the usual "flow" quality. However, the standard heat balance is currently stated as a function of the "flow" quality. Therefore, we should search a new energy balance between the mixture enthalpy, based on "thermodynamic" quality, and the absorbed heat. This work presents the results of such analysis based on accurate measurements of the axial profile of the cross-sectional average void fraction in the region of boiling with subcooling for water at medium and high pressures taken by Moscow Power Institute (MPI) and Argonne National Laboratory (ANL). As main results, we find that, under uniform heat flux, the mixture enthalpy suffers an abrupt reduction of its slope upon passing saturation point, and a new slip ratio could balance heat with such mixture enthalpy. © 2007 American Institute of Chemical Engineers AIChE J, 2007 [source] An Agency Theory Investigation of Supply Risk M anagementJOURNAL OF SUPPLY CHAIN MANAGEMENT, Issue 3 2003George A. Zsidisin SUMMARY Managing supply risk is an essential element of the overall supply management task. As the complexity of risk management has increased, responsiveness seems dominated by varying the level of inventory and using multiple supply sources as means of creating buffers. This research uses the framework of agency theory in managing supplier behaviors as a means to reduce supply risk and the impact of detrimental events. Empirical results indicate that purchasing organizations address various sources of supply risk by implementing management techniques that reduce the likelihood that detrimental events will occur. Firm size, purchases as a percentage of sales, and industry characteristics were also found to influence the manner in which supplier behaviors are managed. [source] The Politics of Privatization in Russia: From Mass Privatization to the Yukos AffairPACIFIC FOCUS, Issue 1 2006Duckjoon Chang Privatization constitutes one of the most successful achievements in Post-Soviet Russian reform. However, apparent great successes notwithstanding, the privatization program tainted with distortions of its original ideas, political compromises and collusions between political leaders and business elites produced tremendous criticisms and distrust as well. Given those negative aspects of privatization, some people raised the necessity of review of the privatization programs conducted during the 1990s. But despite such criticisms and negative evaluations of the privatization program, as was shown in the case of the Yukos affair, the Russian government never denied the principle of private ownership nor reexamined the privatization results. To explain such a trend in Russian privatization, this paper adopts the concept of policy learning, in which reconceptualization of policy agendas-adopting private property as an essential element of the market economy, for example-take place. [source] Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraintsPROTEIN SCIENCE, Issue 3 2003Francesca M. Marassi Abstract A solid-state NMR approach for simultaneous resonance assignment and three-dimensional structure determination of a membrane protein in lipid bilayers is described. The approach is based on the scattering, hence the descriptor "shotgun," of 15N-labeled amino acids throughout the protein sequence (and the resulting NMR spectra). The samples are obtained by protein expression in bacteria grown on media in which one type of amino acid is labeled and the others are not. Shotgun NMR short-circuits the laborious and time-consuming process of obtaining complete sequential assignments prior to the calculation of a protein structure from the NMR data by taking advantage of the orientational information inherent to the spectra of aligned proteins. As a result, it is possible to simultaneously assign resonances and measure orientational restraints for structure determination. A total of five two-dimensional 1H/15N PISEMA (polarization inversion spin exchange at the magic angle) spectra, from one uniformly and four selectively 15N-labeled samples, were sufficient to determine the structure of the membrane-bound form of the 50-residue major pVIII coat protein of fd filamentous bacteriophage. Pisa (polarity index slat angle) wheels are an essential element in the process, which starts with the simultaneous assignment of resonances and the assembly of isolated polypeptide segments, and culminates in the complete three-dimensional structure of the protein with atomic resolution. The principles are also applicable to weakly aligned proteins studied by solution NMR spectroscopy. [The structure we determined for the membrane-bound form of the Fd bacteriophage pVIII coat protein has been deposited in the Protein Data Bank as PDB file 1MZT.] [source] A further proteomic study on the effect of iron in the human pathogen Trichomonas vaginalisPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 12 2007Jose Batista De Jesus Dr. Abstract Iron is an essential element to support the growth and survival of Trichomonas vaginalis. It plays a critical role in the host,parasite interaction, and modulates the expression of virulence factors in this protozoan. In this work, parasites grown in iron-rich and iron-depleted media were analyzed by (i) light and scanning electron microscopy and (ii) 2-DE and MS. Withdrawal of iron from the culture medium resulted in dramatic changes in both the morphology and in the proteome pattern of T. vaginalis. Trophozoites underwent transformation from ellipsoid or amoeboid forms to rounded cells, whose flagella and axostyle were internalized. Forty-five proteins differentially expressed in parasites cultivated in the absence of iron were identified. In iron-depleted parasites, enzymes involved in energetic metabolism, proteolysis and hydrogenosomal iron-sulfur (Fe-S) proteins were down-regulated or even suppressed. Among up-regulated proteins, six isoforms of actin were detected. In addition, phosphoenolpyruvate carboxykinase, putative lactate dehydrogenase, and putative adenosine triphosphatase were also up-regulated or were exclusively observed in gels related to iron-depleted parasites. Our data demonstrate that iron has a pivotal role in the regulation of the morphological transformation of T. vaginalis and modulates the expression of both Fe-S and non-Fe-S proteins in the parasite. [source] Lactational programming? mother's milk energy predicts infant behavior and temperament in rhesus macaques (Macaca mulatta)AMERICAN JOURNAL OF PRIMATOLOGY, Issue 6 2010Katie Hinde Abstract There are many aspects of "mothering" that may provide information to the mammalian infant about environmental conditions during critical periods of development. One essential element of mothering involves the quantity and quality of milk that mothers provide for their infants, but little is known about the consequences of variation in milk production. Mother's milk may affect infant behavior by contributing to brain development and to the development of behavioral dispositions. Here we present the first evidence for any mammal that natural variation in available milk energy (AME) from the mother is associated with later variation in infant behavior and temperament in rhesus macaques (Macaca mulatta, N=59). In the early postnatal period, heavier mothers with more reproductive experience produced greater AME, which is the product of milk energy density (kcal/g) and milk yield (g). Moreover, infants whose mothers produced greater AME in the early postnatal period showed higher activity levels and greater confidence in a stressful setting later in infancy. Our results suggest that the milk energy available soon after birth may be a nutritional cue that calibrates the infant's behavior to environmental or maternal conditions. These data provide new insight into potential mechanisms for the development of behavior and temperament and illuminate new directions for investigating maternal effects, nutritional programming, and developmental plasticity. Am. J. Primatol. 72:522,529, 2010. © 2010 Wiley-Liss, Inc. [source] Grievance: The underlying oedipal configurationTHE INTERNATIONAL JOURNAL OF PSYCHOANALYSIS, Issue 4 2008Michael Feldman In this paper the author describes some of the clinical features encountered in patients who seem to ,nurture' a persistent grievance. He gives clinical examples, and discusses the nature of the powerful underlying dynamics. He suggests that contained within the patient's grievance is a set of phantasies that constitute the expression of his fear and hatred of reality, particularly the reality of the oedipal situation, the child's relationship to the creative parental couple, which Money-Kyrle (1968, 1971) has characterised as an essential element of ,the facts of life'. The phantasies the patient has evolved serve to protect him from envy and jealousy, anxiety and guilt. The primitive oedipal phantasies on which the grievance rests also contribute to the excitement and gratification that are characteristic of the grievance. The analysis of the underlying state of mind helps to account for the persistent grip the grievance has on the patient, and the way this interferes with development. [source] Silicon: its manifold roles in plantsANNALS OF APPLIED BIOLOGY, Issue 2 2009E. Epstein Abstract The title of this essay declares that silicon does have roles in plants and all participants in this conference know that that is so. This knowledge, however, is not shared by the general community of plant biologists, who largely ignore the element. This baffling contrast is based on two sets of experience. First, higher plants can grow to maturity in nutrient solutions formulated without silicon. That has led to the conventional wisdom that silicon is not an essential element, or nutrient, and thus can be disregarded. Second, the world's plants do not grow in the benign environment of solution culture in plant biological research establishments. They grow in the field, under conditions that are often anything but benign. It is there, in the real world with its manifold stressful features, that the silicon status of plants can make a huge difference in their performance. The stresses that silicon alleviates range all the way from biotic, including diseases and pests, to abiotic such as gravity and metal toxicities. Silicon performs its functions in two ways: by the polymerization of silicic acid leading to the formation of solid amorphous, hydrated silica, and by being instrumental in the formation of organic defence compounds through alteration of gene expression. The silicon nutrition of plants is not only scientifically intriguing but also important in a world where more food will have to be wrung from a finite area of land, for that will put crops under stress. 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