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Escherichia Coli ATCC (escherichia + coli_atcc)
Selected AbstractsChemical composition and inhibitory effect of essential oil and organic extracts of Cestrum nocturnum L. on food-borne pathogensINTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 6 2009Sharif M. Al-Reza Summary In this study, we examined the chemical compositions of essential oil and tested the efficacy of oil and organic extracts of Cestrum nocturnum L. against food-borne pathogens. The chemical compositions of the oil was analysed by gas chromatography-mass spectrometry (GC-MS). Forty-seven compounds representing 93.28% of the total oil were identified. The oil [5 ,L of 1:5 (v/v) dilution of oil with methanol] and organic extracts of hexane, chloroform, ethyl acetate and methanol (300 ,g per disc) of C. nocturnum displayed a great potential of antibacterial activity against Staphylococcus aureus (ATCC 6538 and KCTC 1916), Listeria monocytogenes (ATCC 19166 and ATCC 15313), Bacillus subtilis ATCC 6633, Pseudomonas aeruginosa KCTC 2004, Salmonella typhimurium KCTC 2515 and Escherichia coli ATCC 8739. Also the oil had strong detrimental effect on the viable count of the tested bacteria. The results obtained from this study may contribute to the development of new antimicrobial agents with potential applications in food industries as natural preservatives to control food-borne pathogens. [source] Effect of pulsed ultrasound in combination with gentamicin on bacterial viability in biofilms on bone cements in vivoJOURNAL OF APPLIED MICROBIOLOGY, Issue 3 2005G.T. Ensing Abstract Aims:, The aim of this study is to investigate whether pulsed ultrasound (US) in combination with gentamicin yields a decreased viability of bacteria in biofilms on bone cements in vivo. Methods and Results:, Bacterial survival on bone cement in the presence and absence of ultrasound was compared in a rabbit model. Two bone cement samples with an Escherichia coli ATCC 10798 biofilm were implanted in a total of nine rabbits. In two groups bone cement discs loaded with gentamicin, freshly prepared and aged were used, and in one group unloaded bone cement discs in combination with systemically administered gentamicin. Pulsed ultrasound with a frequency of 28·48 kHz and a maximum acoustic intensity of 500 mW cm,2 was applied continuously from 24 h till 72 h postsurgery on one of the two implanted discs. After euthanization and removal of the bacteria from the discs, the number of viable bacteria were quantified and skin samples were analysed for histopathological examination. Application of ultrasound, combined with gentamicin, reduced the viability of the biofilms in all three groups varying between 58 and 69% compared with the negative control. Histopathological examinations showed no skin lesions. Conclusions:, Ultrasound resulted in a tendency of improved efficacy of gentamicin, either applied locally or systemically. Usage of ultrasound in this model proved to be safe. Significance and Impact of the Study:, This study implies that ultrasound could improve the prevention of infection immediately after surgery, especially because the biomaterials, gentamicin and ultrasound used in this model are all in clinical usage, but not yet combined in clinical practice. [source] New Antimicrobial Materials Based on Polymers With Nanostructured Surface Modified by Organic Fullerene[60] DerivativesPLASMA PROCESSES AND POLYMERS, Issue S1 2009Vera M. Ellinson Abstract In the present work, the surface of natural and artificial polymers was modified by plasma treatment using reactive gases mixture, ion-beam-induced deposition of , -C:H films and organic C60 derivatives deposition. The study of prepared biomaterials with nanostructured surface was carried out by means of atomic force microscopy, X-ray photoelectron spectroscopy, contact angle measurement and surface energy calculation. The investigation of antimicrobial activity of the materials was carried out by application method in relation to Gram-positive (Staphylococcus aureus ATCC 29213) and Gram-negative (Escherichia coli ATCC 54383 and Pseudomonas aeruginosa ATCC 27853) microorganisms and fungi (Candida albicans). [source] Synthesis, Antibacterial and Antifungal Activities of Novel 1,2,4-Triazolium DerivativesARCHIV DER PHARMAZIE, Issue 7 2009Yan Luo Abstract A series of novel 1,2,4-triazolium derivatives was synthesized starting from commercially available 1H -1,2,4-triazole, 2,4-dichlorobenzyl chloride, or 2,4-difluorobenzyl bromide. Their antibacterial and antifungal activities were evaluated against Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Bacillus proteus, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans ATCC 76615, and Aspergillus fumigatus. All structures of the new compounds were confirmed by NMR, IR, and MS spectra, and elemental analyses. The antimicrobial tests showed that most of synthesized triazolium derivatives exhibit significant antibacterial and antifungal activities in vitro. 1-(2,4-Difluorobenzyl)-4-dodecyl-1H -1,2,4-triazol-4-ium bromide and 1-(2,4-Dichlorobenzyl)-4-dodecyl-1H -1,2,4- triazol-4-ium bromide were the most potent compounds against all tested strains with the MIC values ranging from 1.05 to 8.38 ,M. They exhibited much stronger activities than the standard drugs chloramphenicol and fluconazole which are in clinical use. The results also showed that the antimicrobial activities of triazolium derivatives depend upon the type of substituent, the length of the alkyl chain, and the number of triazolium rings. [source] New indolicidin analogues with potent antibacterial activity,CHEMICAL BIOLOGY & DRUG DESIGN, Issue 5 2004T.S. Ryge Abstract:, Indolicidin is a 13-residue antimicrobial peptide amide, ILPWKWPWWPWRR-NH2, isolated from the cytoplasmic granules of bovine neutrophils. Indolicidin is active against a wide range of microorganisms and has also been shown to be haemolytic and cytotoxic towards erythrocytes and human T lymphocytes. The aim of the present paper is two-fold. First, we examine the importance of tryptophan in the antibacterial activity of indolicidin. We prepared five peptide analogues with the format ILPXKXPXXPXRR-NH2 in which Trp-residues 4,6,8,9,11 were replaced in all positions with X = a single non-natural building block; N -substituted glycine residue or nonproteinogenic amino acid. The analogues were tested for antibacterial activity against both Staphylococcus aureus American type culture collection (ATCC) 25923 and Escherichia coli ATCC 25922. We found that tryptophan is not essential in the antibacterial activity of indolicidin, and even more active analogues were obtained by replacing tryptophan with non-natural aromatic amino acids. Using this knowledge, we then investigated a new principle for improving the antibacterial activity of small peptides. Our approach involves changing the hydrophobicity of the peptide by modifying the N-terminus with a hydrophobic non-natural building block. We prepared 22 analogues of indolicidin and [Phe4,6,8,9,11] indolicidin, 11 of each, carrying a hydrophobic non-natural building block attached to the N-terminus. Several active antibacterial analogues were identified. Finally, the cytotoxicity of the analogues against sheep erythrocytes was assessed in a haemolytic activity assay. The results presented here suggest that modified analogues of antibacterial peptides, containing non-natural building blocks, are promising lead structures for developing future therapeutics. [source] | |||||||||||||