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Enriched Library (enriched + library)
Selected AbstractsDevelopment and characterization of 30 polymorphic microsatellite markers for the Atlantic surfclam, Spisula solidissima (Dillwyn, 1817)MOLECULAR ECOLOGY RESOURCES, Issue 4 2009YAN WANG Abstract Thirty polymorphic microsatellite markers were developed for the Atlantic surfclam, Spisula solidissima, from an enriched library and characterized in 24 clams from a wild population. The number of alleles ranged from 3 to 16 per locus. The expected and observed heterozygosities ranged from 0.1942 to 0.9238 and 0.0833 to 0.875 respectively. Six loci showed significant (P < 0.05 after Bonferroni correction) deviation from Hardy,Weinberg equilibrium, probably because of the presence of null alleles. Three primer pairs amplified duplicated loci with two involving tandem mini-satellite repeats. Most of the microsatellite markers developed here should be useful for genetic studies in this species. [source] Isolation and characterization of microsatellite loci in tropical forage Stylosanthes capitata VogelMOLECULAR ECOLOGY RESOURCES, Issue 1 2009M. O. SANTOS Abstract Stylosanthes capitata is an important tropical pasture legume. Knowledge of genetic diversity and structure of S. capitata populations is of great importance for the conservation and germplasm management of this species. Thus, eight microsatellite markers were developed from an S. capitata -enriched library. They were characterized in 20 accessions from the germplasm collection of the Empresa Brasileira de Pesquisa Agropecuária (Embrapa). The observed and expected heterozygosities ranged from 0.16 to 0.85 and from 0.40 to 0.85, respectively. These microsatellites are the first set of molecular markers from this species and will contribute towards studies of genetic diversity, conservation and breeding of S. capitata. [source] Isolation and characterization of microsatellite loci from Chiasmia assimilis (Warren, 1899) (Lepidoptera: Geometridae)MOLECULAR ECOLOGY RESOURCES, Issue 3 2004T. J. WARDILL Abstract Twelve microsatellite loci are presented for the biological control agent Chiasmia assimilis (Warren, 1899). These microsatellite loci were obtained through the construction of an enriched library, overcoming previous reported difficulties with obtaining microsatellites from other Lepidoptera due to the low frequency of microsatellites in their genomes. [source] Microsatellite markers for diverse Salix speciesMOLECULAR ECOLOGY RESOURCES, Issue 1 2003J. H. A. Barker Abstract Forty-six microsatellites were isolated from an enriched library of Salix burjatica and tested on 20 individuals (of nine species/hybrids) from the National Willows Collection (IACR-Long Ashton Research Station, UK). Twenty-nine were monomorphic, gave multilocus or unscorable patterns, or were duplicates. The remaining 17 microsatellites gave 2,22 alleles/locus. Three microsatellites successfully cross-amplified in 31 additional Salix species. A further six were tested on panels comprising 6,25 individuals from the 31 species. Cross-amplification was successful in all cases. These results suggest that the microsatellites isolated here should prove useful for population studies in a wide range of Salix species. [source] Microsatellite markers in peach [Prunus persica (L.) Batsch] derived from an enriched genomic and cDNA librariesMOLECULAR ECOLOGY RESOURCES, Issue 3 2002T. Yamamoto Abstract Twenty-four and 12 microsatellite loci were developed in peach [Prunus persica (L) Batsch cv. Akatsuki] by using an enriched genomic and fruit cDNA libraries, respectively. The microsatellite loci obtained from an enriched library produced 1,9 alleles per locus, 24 in total, of which 22 showed polymorphisms. The average values of observed and expected heterozygosities among the 24 loci were 0.15 and 0.68, respectively. The microsatellite loci derived from cDNA showed 1,7 alleles per locus. Eight sequences showed significant homology to the registered genes in a database. [source] Development and characterization of SSR markers in Ribes speciesMOLECULAR ECOLOGY RESOURCES, Issue 3 2002R. Brennan Abstract Eleven microsatellite loci were identified and characterized in blackcurrant (Ribes nigrum L.) and related species. An enriched library was constructed and screened with simple sequence repeat (SSR) oligonucleotides. Positive clones were sequenced and primers were designed flanking the repeat motifs. These 11 microsatellites produce amplification products polymorphic across a range of Ribes germplasm, predominantly from the Eucoreosma section of the genus. The number of alleles varied from 2 to 18 with levels of diversity ranging from 0.18 to 0.91. [source] Microsatellites for the net-spinning caddisfly Plectrocnemia conspersa (Polycentropodidae)MOLECULAR ECOLOGY RESOURCES, Issue 4 2001H. R. Wilcock Abstract Six polymorphic microsatellites were developed for the caddisfly Plectrocnemia conspersa from two enriched partial genomic libraries. These represent the first microsatellites published for the order Trichoptera. We show that, whilst it is possible to develop a highly enriched library, isolation of polymorphic microsatellites is difficult for this species, as has been found in some other of invertebrate groups. The genotypes of 160 individuals were determined using these new loci. Observed heterozygosities ranged from 0.013 to 0.788. Despite their problematic isolation, polymorphic microsatellite loci show greater levels of variation than previously studied allozyme markers. [source] A chicken linkage map based on microsatellite markers genotyped on a Japanese Large Game and White Leghorn crossANIMAL GENETICS, Issue 6 2005H. Takahashi Summary A detailed linkage map is necessary for efficient detection of quantitative trait loci (QTL) in chicken resource populations. In this study, microsatellite markers isolated from a (CA)n -enriched library (designated as ABR Markers) were mapped using a population developed from a cross between Japanese Game and White Leghorn chickens. In total, 296 markers including 193 ABR, 43 MCW, 31 ADL, 22 LEI, 3 HUJ, 2 GCT, 1 UMA and 1 ROS were mapped by linkage to chicken chromosomes 1,14, 17,21, 23, 24, 26,28 and Z. In addition, five markers were assigned to the map based on the chicken draft genomic sequence, bringing the total number of markers on the map to 301. The resulting linkage map will contribute to QTL mapping in chicken. [source] | ||||||||||