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EDTA Solution (edta + solution)
Selected AbstractsDistribution of SIBLING proteins in the organic and inorganic phases of rat dentin and boneEUROPEAN JOURNAL OF ORAL SCIENCES, Issue 2 2008Bingzhen Huang The SIBLING protein family is a group of non-collagenous proteins (NCPs) that includes dentin sialophosphoprotein (DSPP), dentin matrix protein 1 (DMP1), bone sialoprotein (BSP), and osteopontin (OPN). In the present study, we compared these four proteins in different phases of rat dentin and bone. First, we extracted NCPs in the unmineralized matrices and cellular compartments using guanidium-HCl (G1). Second, we extracted NCPs closely associated with hydroxyapatite using an EDTA solution (E). Last, we extracted the remaining NCPs again with guanidium-HCl (G2). Each fraction of Q-Sepharose ion-exchange chromatography was analyzed using sodium dodecyl sulfate,polyacrylamide gel electrophoresis (SDS,PAGE), Stains-All stain, and with western immunoblotting. In dentin, the NH2 -terminal fragment of DSPP and its proteoglycan form were primarily present in the G1 extract, whereas the COOH-terminal fragment of DSPP was present exclusively in the E extract. The processed NH2 -terminal fragment of DMP1 was present in G1 and E extracts, whereas the COOH-terminal fragment of DMP1 existed mainly in the E extract. Bone sialoprotein was present in all three extracts of dentin and bone, whereas OPN was present only in the G1 and E extracts of bone. The difference in the distribution of the SIBLING proteins between organic and inorganic phases supports the belief that these molecular species play different roles in dentinogenesis and osteogenesis. [source] Dentine demineralization when subjected to EDTA with or without various wetting agents: a co-site digital optical microscopy studyINTERNATIONAL ENDODONTIC JOURNAL, Issue 4 2008G. De-Deus Abstract Aim, To analyse quantitatively the chelating ability of ethylenediaminetetraacetic acid (EDTA) and three common EDTA-based associations with wetting agents. Methodology, Twelve maxillary human molars were selected, from which 3 mm thick discs were obtained from the cervical third of the root. Following the creation of standardized smear layer co-site microscopy image sequences of the dentine surface submitted to EDTA, EDTA plus 0.1% cetavlon® (Sigma Chemical Co., St Louis, MO, USA), EDTA plus 1.25% sodium lauryl ether sulphate and SmearClearÔ (Sybron Endo, Orange, CA, USA) were obtained after several cumulative demineralization times. Sixteen images were obtained of each dentine sample for each experimental time, at 1000× magnification. An image processing and analysis sequence was used to measure the area of open tubules for each experimental time. Thus, it was possible to follow the demineralization process and quantitatively analyse the effect of the various substances. The Student's t -test was used to assess differences between experimental groups. Results, EDTA solution had the strongest effect at all experimental times whilst the association of EDTA with wetting agents showed a weaker chelating effect and this difference was statistically significant (P < 0.05). Conclusions, (i) The EDTA solution had the strongest effect at all experimental times (P < 0.05); (ii) the association of EDTA with wetting agents did not improve the chelating power of the solution; (iii) co-site optical microscopy represents a powerful approach to compare directly, longitudinally and quantitatively the ability of the chelating solutions. [source] Inhibition of biofilms associated with dentures and toothbrushes by tetrasodium EDTAJOURNAL OF APPLIED MICROBIOLOGY, Issue 6 2007D.A. Devine Abstract Aims:, We examined the efficacy of tetrasodium EDTA in eradicating biofilms derived from salivary inocula or pure cultures of Candida albicans on discs of polymethyl methacrylate (PMMA) denture base or on toothbrushes that had been used normally for 4,8 weeks. Its efficiency in virus neutralization was also determined. Methods and Results:, Overnight (16 h) treatment with 4% (w/v) tetrasodium EDTA solution reduced salivary and C. albicans biofilm viable counts by ,99%. Biofilm removal was confirmed using confocal laser scanning microscopy. Presence/absence of sucrose during biofilm formation had no effect on killing efficacy. Prolonged treatment of PMMA with tetrasodium EDTA did not influence subsequent formation of C. albicans biofilms or affect surface roughness of the PMMA, but it reduced subsequent biofilm formation from a salivary inoculum. Infectivities of herpes simplex virus and polio virus suspensions were reduced by >99·99% by treatment for 1 and 2 h, respectively. Conclusions:, Tetrasodium EDTA solution efficiently disinfected toothbrushes and PMMA discs, with the detachment of biofilms, and rapidly neutralized both nonenveloped and enveloped viruses. Significance and Impact of the Study:, Dentures and toothbrushes become contaminated by bacterial biofilms and by viruses. There is a need for disinfection methods that are rapidly effective, cost-effective, nontoxic and easily implemented. These studies indicate that tetrasodium EDTA solution has disinfection applications in the oral care field. [source] A simple and efficient method for extraction of PCR-amplifiable DNA from chicken eggshellsANIMAL SCIENCE JOURNAL, Issue 2 2009Kazuhiro RIKIMARU ABSTRACT Recently, we reported a method for discriminating a Japanese brand of chicken, the Hinai-jidori. As an application of this method for discriminating Hinai-jidori eggs, we here report an efficient method for extracting maternal DNA from eggshells. Eggshell powder was completely decalcified with EDTA solution, and then DNA was isolated by conventional phenol-chloroform extraction and ethanol precipitation. The efficiency of DNA recovery from eggshells was 50-fold higher than that of a previously reported method. The recovered DNA could be used for PCR, and 10 markers for identifying the Hinai-jidori chicken were detected. The genotypes of the Hinai-jidori exactly matched those of the Hinai-dori breed. Using this method, Hinai-jidori and Hinai-dori eggs could be distinguished from the eggs of Rhode Island Reds. This is the first report of a technique that can be used to discriminate the eggs of Hinai-jidori from those of other chickens, and it can also be utilized to validate the labeling of Hinai-jidori eggs in the market. [source] | |||||||||||||