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Duplicate Groups (duplicate + groups)
Selected AbstractsEffects of substitution of dietary fish oil with a blend of vegetable oils on liver and peripheral blood leucocyte fatty acid composition, plasma prostaglandin E2 and immune parameters in three strains of Atlantic salmon (Salmo salar)AQUACULTURE NUTRITION, Issue 6 2009I.K. PETROPOULOS Abstract Duplicate groups of three genetic strains of Atlantic salmon smolts were cultured on diets containing either fish oil (FO) or a blend of vegetable oils (VO). Fatty acid compositions of liver and peripheral blood leucocytes were compared. The effect of different strains and diets on innate immune parameters and plasma prostaglandin E2 were also measured. Two strains were selected as being either ,fat' or ,lean' in terms of muscle adiposity. The third strain was a commercial stock (MH). Total replacement of dietary FO with VO resulted in reduced docosahexaenoic (DHA; 22:6n -3) and eicosapentaenoic acids (EPA; 20:5n -3) in liver, while oleic (18:1n -9), linoleic (18:2n -6) and ,-linolenic (18:3n -3) acids were all increased in VO-fed fish. Fatty acid compositions of blood leucocytes showed similar changes. Evaluation of innate immune function showed that in the fat strain, circulating leucocytes were significantly lower in VO fish. The lean strain also had significantly higher serum lysozyme activity than MH fish. Reduced haematocrit was seen in VO lean fish compared with FO lean fish. This study provides evidence of strain-induced differences in liver and leucocyte fatty acid compositions and innate immunity in Atlantic salmon fed either FO- or VO-based diets. [source] Dietary menadione nicotinamide bisulphite (vitamin K3) does not affect growth or bone health in first-feeding fry of Atlantic salmon (Salmo salar L.)AQUACULTURE NUTRITION, Issue 6 2009C. KROSSØY Abstract The aim of the present study was to elucidate if increasing levels of added vitamin K in the feed influenced fish growth, health or the incidences of bone deformities in Atlantic salmon (Salmo salar L.) from onset of start feeding to ,100 g in freshwater. Duplicate groups of Atlantic salmon fry (,0.20 g) were fed eight levels (0, 2.5, 5, 7.5, 10, 15, 20 and 50 mg menadione kg,1) of the vitamin K derivate menadione nicotinamide bisulphite (MNB) in a regression design for 28 weeks. All fish maintained high growth rates throughout the experiment, and showed no significant differences in specific growth rate, condition factor, whole body proximate analysis, blood coagulation time, vertebra morphology or mechanical properties of vertebrae. We found a dose-response between whole body vitamin K concentration and the dietary MNB supplementation level. Analysis of liver ,-glutamylcarboxylase activity revealed significant dose-dependent differences between groups given the 0, 10 and 50 mg MNB kg,1 diets. In conclusion, Atlantic salmon seems to require low levels of dietary vitamin K, and the amount of vitamin K found naturally in the presently used feed ingredients may be enough to maintain optimal growth, health and bone strength in Atlantic salmon fry from start feeding. [source] Effects of photoperiod on growth and spawning efficiency of Nile tilapia (Oreochromis niloticus L.) broodstock in a recycling systemAQUACULTURE RESEARCH, Issue 12 2007Abdel-Fattah M El-Sayed Abstract This study was conducted to evaluate the effects of photoperiod on the growth, feed conversion ratio (FCR) and spawning performance of Nile tilapia (Oreochromis niloticus) broodstock. Duplicate groups of two males (66±3.04 g) and six females (50.5±1.58 g) were stocked in 0.4 m3 fibreglass tanks in a recirculating water system at a male:female ratio of 1:3. The fish were subjected to four photoperiod treatments: 24:0 light:dark (L:D), 18L:6D, 12L:12D and 6L:18D. Light intensity was kept constant at about 2500 lx throughout the study. The fish were fed a commercial tilapia diet (35% crude protein and 16.6 MJ GE kg,1) at a daily rate of 2,3% of tank biomass, twice a day, for 130 days. Males grew significantly faster than females under all photoperiod regimes. The best growth rate and FCR were attained at 18L:6D, followed by 24L:0D, 12L:12D and 6L:18D respectively. The number of eggs per female, number of eggs per spawn and number of spawnings per female were all significantly higher in the 12L:12D treatment than in all other photoperiod cycles. Interspawning intervals and days elapsed per spawn were also shorter in the 12L:12D treatment. The time to first spawning was slightly longer in the 24L:0D and 6L:18D than in 12L:12D and 18L:6D light phases. The 18L:6D and 6L:18D photoperiods produced the lowest spawning performance. It is concluded that a 12L:12D photoperiod regime should be adopted for maximum fecundity, seed production and spawning frequencies of Nile tilapia broodstock reared in intensive, recirculating systems. If maximum reproduction is desired, a near-natural day length photoperiod should be used. [source] Dietary lysine requirement of juvenile gilthead seabream Sparus aurata L.,AQUACULTURE NUTRITION, Issue 1 2006P.A. MARCOULI Abstract The dietary lysine requirement of juvenile gilthead seabream was determined by the growth response of duplicate groups of fish (3.5 g initial weight) fed on six isonitrogenous (427 g kg,1) and isolipidic (135 g kg,1) diets containing graded levels of crystalline l -lysine HCl, with dietary lysine content ranging from 36.3 to 79.7 g kg,1 of protein. The final indispensable amino acid profile of the diets except for lysine was formulated so as to resemble that of wild seabream whole body. Except for the reduced growth performance of fish groups fed the lysine-deficient diets no other deficiency signs were apparent. Survival observed throughout the feeding period of 6 weeks was excellent. Weight gain (in %), specific growth rate, feed efficiency and daily protein deposition (DPD) were significantly improved in response to the increasing levels of dietary lysine up to 52.7 g kg,1 of protein and remained nearly constant thereafter. Whole-body protein content followed a similar pattern as growth parameters in relation to dietary lysine level. Non-linear regression analysis of DPD against dietary lysine level using the four-parameter saturation kinetic model indicated a lysine requirement of 50.4 g kg,1 of protein for this species to support growth. [source] Effect of feed restriction on the growth performance of turbot (Scophthalmus maximus L.) juveniles under commercial rearing conditionsAQUACULTURE RESEARCH, Issue 8 2010Isidro Blanquet Abstract Two trials were performed to study the effect of periodic feed deprivation (trial 1) or feed restriction, followed by satiation feeding (trial 2) on the growth performance of turbot juveniles under commercial rearing conditions. In trial 1, duplicate groups of 350 fish with an initial weight of 62 g were fed a commercial diet to apparent visual satiation for 7, 6, 5 or 4 days a week for 83 days. At the end of the trial, fish weight was directly related to the number of feeding days but feed efficiency and protein efficiency ratio were not affected by treatments. At the end of the trial, there were no differences in whole-body composition among groups. In trial 2, duplicate groups of 500 fish with an initial weight of 33 g were fed a commercial diet to satiation (100%) or feed restricted to 90%, 80% and 70% of satiation for 90 days. Thereafter, all groups were fed to satiation for 34 days. During the feed restriction period, growth was directly related to feed intake, while during the satiation feeding period, it was inversely related to the previous feeding level. At the end of the trial, the final weight was not different among groups. At the end of the feed restriction period, whole-body lipid content showed a trend to decrease with an increase in the feed restriction level. The results of this study indicate that under practical conditions, turbot juveniles should be fed daily as even cycles of short periods of feed deprivation negatively affect growth, while not improving feed efficiency. On the contrary, even after a relatively long feed restriction period, fish shows compensatory growth, and this may be used as a feed management strategy for controlling fish production in commercial farms. [source] Effects of dietary carbohydrate to lipid ratios on growth and body composition of juvenile and grower rockfish, Sebastes schlegeliAQUACULTURE RESEARCH, Issue 16 2009Sang-Min Lee Abstract Two feeding trials were conducted to determine the optimal dietary carbohydrate to lipid (CHO:L) ratio for juvenile and grower rockfish. Triplicate groups of juvenile (initial mean weight 3.6 g) and duplicate groups of grower (initial mean weight 166 g) were fed the five isonitrogenous (51% CP) and isoenergetic (4.0 kcal g,1) diets with the different CHO:L ratios (0.4,5.6 g:g) for 8 weeks respectively. The survival of juvenile and grower was above 93% and was not affected by the dietary CHO:L ratios. Weight gain of juvenile fed the diets with CHO:L ratios of 0.8 and 1.6 was significantly higher than that of the fish fed diets with CHO:L ratios of 2.8 and 5.6 (P<0.05). The feed efficiency and protein efficiency ratio of juvenile fed the diet with CHO:L ratio of 5.6 were the lowest among all groups (P<0.05). The daily feed intake of juvenile fed the diet with a CHO:L ratio of 5.6 was significantly higher than that of the other groups (P<0.05). The condition factors of juvenile fed the diets with CHO:L ratios of 0.8 and 1.6 were significantly higher than that of 5.6 (P<0.05). The crude lipid content of whole body, liver and viscera of juvenile decreased as the dietary CHO:L ratio increased, and the opposite was found for the moisture content. Weight gain, feed efficiency, daily feed intake, protein efficiency ratio and condition factor of grower were not affected by the dietary CHO:L ratio. Hepatosomatic and viscerasomatic indexes of grower were significantly influenced by dietary CHO:L ratio (P<0.05). Significant differences were observed in the lipid content of whole body and viscera of grower. Dietary CHO:L ratios significantly affected the major fatty acid composition of whole body in both juvenile and grower. The contents of 18:2n-6 and 18:3n-3 linearly decreased as the dietary CHO:L ratio increased, whereas the 20:4n-6, 20:5n-3 and 22:6n-3 contents increased. Based on growth, feed efficiency and body composition, the optimal dietary CHO:L ratio was 1.6 for juvenile rockfish fed isonitrogenous (51% CP) and isoenergetic (4.0 kcal g,1) diets, and starch could partially replace lipids in the diets with CHO:L ratios ranging from 0.4 to 5.6 for grower. [source] | ||||||||||