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Dramatic Impact (dramatic + impact)
Selected AbstractsTROSY effects in MAS solid-state NMRCONCEPTS IN MAGNETIC RESONANCE, Issue 2 2008Veniamin Chevelkov Abstract Use of transverse relaxation-optimized spectroscopy (TROSY) type techniques had a dramatic impact on the study of large proteins with a molecular weight >30kDa for solution-state NMR. In the solid-state, such an effect would not be expected a prior, as the investigated molecules are immobilized. However, local motions induce fluctuations of the local fields experienced by the nuclear spins and, this way, are effective for relaxation. We demonstrate that protein dynamics can significantly influence the resonance line width in ultra high resolution MAS (magic angle spinning) solid-state NMR experiments. Averaging of the 15NH,/, multiplet components as a consequence of 1H decoupling induces effective broadening of the 15N resonance. Application of TROSY type techniques that select only the narrow component of the multiplet pattern results in an increased resolution and, thus, will be of benefit for MAS solid-state NMR spectroscopy. © 2008 Wiley Periodicals, Inc. Concepts Magn Reson Part A 32A: 143,156, 2008. [source] IS E-GOVERNMENT LEADING TO MORE ACCOUNTABLE AND TRANSPARENT LOCAL GOVERNMENTS?FINANCIAL ACCOUNTABILITY & MANAGEMENT, Issue 1 2010AN OVERALL VIEW This paper seeks to assess to what extent e-government enables accountability and transparency in EU local governments. It also provides an overall view about how local governments are implementing ICTs initiatives to bring citizens closer to governments. Although the mere capacity of the Internet for the dissemination of information improves accountability and makes benchmarking easier, our results show that the expected benefits are far from being achieved because e-government projects are still in the early stages. The results also show that, at present, ICTs have not had a dramatic impact on EU local government accountability. [source] The impact of Sirococcus shoot blight on radial and height growth of Norway spruce (Picea abies) in young plantationsFOREST PATHOLOGY, Issue 3 2000E. Halmschlager Summary The impact of Sirococcus shoot blight on the radial and height growth of Norway spruce in a young plantation (approximately 20 years old) was investigated by examining the increment losses for four infection intensities (classes). The average diameter at breast height of trees in the lightly damaged class was 72% when compared with the average diameter of the healthy trees, whereas moderately and severely damaged tree classes were 67 and 57%, respectively. Using tree ring analysis, the development of radial growth over time due to intensity of infection was studied. Height growth of affected trees was also significantly reduced (up to 43%) compared with the healthy trees, thus indicating a dramatic impact of Sirococcus conigenus on the growth of young Norway spruce. [source] Predicting the impacts of climate change on the distribution of species: are bioclimate envelope models useful?GLOBAL ECOLOGY, Issue 5 2003Richard G. Pearson ABSTRACT Modelling strategies for predicting the potential impacts of climate change on the natural distribution of species have often focused on the characterization of a species' bioclimate envelope. A number of recent critiques have questioned the validity of this approach by pointing to the many factors other than climate that play an important part in determining species distributions and the dynamics of distribution changes. Such factors include biotic interactions, evolutionary change and dispersal ability. This paper reviews and evaluates criticisms of bioclimate envelope models and discusses the implications of these criticisms for the different modelling strategies employed. It is proposed that, although the complexity of the natural system presents fundamental limits to predictive modelling, the bioclimate envelope approach can provide a useful first approximation as to the potentially dramatic impact of climate change on biodiversity. However, it is stressed that the spatial scale at which these models are applied is of fundamental importance, and that model results should not be interpreted without due consideration of the limitations involved. A hierarchical modelling framework is proposed through which some of these limitations can be addressed within a broader, scale-dependent context. [source] Relationship marketing, audience retention and performing arts organisation viabilityINTERNATIONAL JOURNAL OF NONPROFIT & VOLUNTARY SECTOR MARKETING, Issue 2 2002Ruth Rentschler Marketing strategy in performing arts organisations has become particularly important in the increasingly competitive environment in which the arts operate. Since the late 1980s there has been a necessary shift in focus to audience development away from product development. This change in focus is being encouraged to ensure the long-term viability of performing arts organisations (PAOs) and micro-economic reform. While government reports have recommended strategies aimed at building audience-based recognition, this is an expensive approach for many PAOs and does not produce short-term returns. Little attention has been paid to building enduring relationships with existing audiences as a way of having a more dramatic impact on PAOs' long-term viability. This paper explores this theme through relationship marketing and the implication of retaining existing audiences. The paper identifies the changing cultural environment which has led to the importance of marketing. It then explains the concepts of relationship marketing and its pertinence to PAOs' viability by presenting a loyalty ladder. The structure is modelled as a dynamic conceptualisation of the relationships (audience and organisation) to assist arts managers to decide whether to focus their efforts on catching or keeping customers to maximise earned income. Copyright © 2002 Henry Stewart Publications [source] New approaches to understanding late Quaternary climate fluctuations and refugial dynamics in Australian wet tropical rain forestsJOURNAL OF BIOGEOGRAPHY, Issue 2 2009Jeremy VanDerWal Abstract Aim, We created spatially explicit models of palaeovegetation stability for the rain forests of the Australia Wet Tropics. We accounted for the climatic fluctuations of the late Quaternary, improving upon previous palaeovegetation modelling for the region in terms of data, approach and coverage of predictions. Location, Australian Wet Tropics. Methods, We generated climate-based distribution models for broad rain forest vegetation types using contemporary and reconstructed ,pre-clearing' vegetation data. Models were projected onto previously published palaeoclimate scenarios dating to c. 18 kyr bp. Vegetation stability was estimated as the average likelihood that a location was suitable for rain forest through all climate scenarios. Uncertainty associated with model projections onto novel environmental conditions was also tracked. Results, Upland rain forest was found to be the most stable of the wet forest vegetation types examined. We provide evidence that the lowland rain forests were largely extirpated from the region during the last glacial maximum, with only small, marginally suitable fragments persisting in two areas. Models generated using contemporary vegetation data underestimated the area of environmental space suitable for rain forest in historical time periods. Model uncertainty resulting from projection onto novel environmental conditions was low, but generally increased with the number of years before present being modelled. Main conclusions, Climate fluctuations of the late Quaternary probably resulted in dramatic change in the extent of rain forest in the region. Pockets of high-stability upland rain forest were identified, but extreme bottlenecks of area were predicted for lowland rain forest. These factors are expected to have had a dramatic impact on the historical dynamics of population connectivity and patterns of extinction and recolonization of dependent fauna. Finally, we found that models trained on contemporary vegetation data can be problematic for reconstructing vegetation patterns under novel environmental conditions. Climatic tolerances and the historical extent of vegetation may be underestimated when artificial vegetation boundaries imposed by land clearing are not taken into account. [source] Magnetic Resonance Imaging Monitoring of Multiple Sclerosis Lesion EvolutionJOURNAL OF NEUROIMAGING, Issue 2005Matilde Inglese MD ABSTRACT The characteristic feature of multiple sclerosis (MS) pathology is the demyelinated plaque distributed throughout the central nervous system. Although MS is a primary demyelinating disease, acute axonal injury is common in actively demyelinating MS lesions and it is considered one of the major determinants of neurological deficit. Magnetic resonance imaging (MRI) has had a dramatic impact on MS in both the clinical practice and basic science settings. Techniques such as T2-weighted and gadolinium-enhanced T1-weighted MRI are very sensitive in detecting lesions and, thus, increase the level of certainty of MS diagnosis. Conventional MRI has also improved our understanding of the pathogenesis of the disease and has provided objective and reliable measures to monitor the effect of experimental treatments in clinical trials. However, conventional MR,I does not provide specific information on the heterogeneous pathologic substrate of MS lesions. Advanced MRI techniques, such as magnetization transfer imaging, diffusion tensor imaging, and proton MR spectroscopy, offer the unprecedented ability to observe and quantify pathological changes in lesions and normal-appearing brain tissue over time. The present review will discuss the major contributions of conventional MRI and quantitative MRI techniques to understand how individual MS lesions evolve. [source] Effect of relative solubility on amino acid crystal purityAICHE JOURNAL, Issue 12 2001Jeffrey C. Givand The ratio of the pure-component solubility of a primary solute to that of an impurity in the same solvent has a dramatic impact on crystal purity when lattice substitution is the dominant mechanism of impurity incorporation in the crystal. This is demonstrated for a model system of isoleucine and leucine in which leucine is the impurity. The pure-component solubility ratios of isoleucine to leucine in mixed solvents were measured and used to select solvents that could affect the lattice substitution of leucine in isoleucine crystals. Addition of electrolytes and/or cosolvents to aqueous solutions of the two amino acids improved the purity of isoleucine crystals obtained by cooling. [source] Thermal stability of vaccinesJOURNAL OF PHARMACEUTICAL SCIENCES, Issue 2 2003Duane T. Brandau Abstract Worldwide vaccination programs against infectious diseases and toxins are estimated to save approximately 3 million lives yearly. Tragically, however, another 3 million individuals (primarily children) die of vaccine-preven diseases. A significant portion of this problem results from the thermal instability of many of the currently used vaccines. This review argues that modern methods of physical and chemical analysis permit for the first time characterization of the degradative pathways of thermally labile vaccines. A rigorous description of these pathways permit a more rational and systematic approach to the stabilization of vaccines. A direct result of the replacement of currently employed, primarily empirical, approaches to vaccine stabilization with a more molecular-based methodology should be the development of more universally available vaccinations against life-threatening diseases. This has the potential to have a dramatic impact on world health. © 2003 Wiley-Liss, Inc. and the American Pharmaceutical Association J Pharm Sci 92:218,231, 2003 [source] INVESTMENT RISK AND THE TRANSITION INTO HOMEOWNERSHIP,JOURNAL OF REGIONAL SCIENCE, Issue 2 2007Tracy M. Turner R0; D12; D84 ABSTRACT This paper investigates the extent to which house,price uncertainty affects the transition of renter households into homeownership. Using a 14-year household panel from the Panel Study of Income Dynamics during the years 1984,1997 and measures of the time-varying risk and return to owner-occupied housing, we estimate a Cox proportional hazard model of the effect of house,price volatility on the transition into homeownership. Results indicate that house,price uncertainty has a negative and dramatic impact on transitions into homeownership. In addition, we find that the low-wealth renters are particularly sensitive to house,price risk. [source] Modern statistics: the myth and the magicJOURNAL OF THE ROYAL STATISTICAL SOCIETY: SERIES A (STATISTICS IN SOCIETY), Issue 2 2009David J. Hand Summary., The paper is a personal exploration of the puzzling contradiction between the fundamental excitement of statistics and its poor public image. It begins with the historical foundations and proceeds through the role of applications and the dramatic impact of the computer in shaping the discipline. The mismatch between the reality of statistics and its public perception arises from a number of dichotomies, some of which are explored. In particular, although statistics is perhaps typically seen as an impersonal discipline, in some sense it is very personal, and many of its applications are aimed at providing unique benefit to individuals. This benefit depends on the creation of detailed data sets describing individuals, but the contrary view is that this represents an invasion of privacy. Some observations on statistical education are made, and issues which will affect the future health of the discipline are examined. [source] Microsatellite variation within and among North American lineages of Phragmites australisMOLECULAR ECOLOGY, Issue 7 2003K. Saltonstall Abstract Over the past century, the spread of the common reed (Phragmites australis) has had a dramatic impact on wetland communities across North America. Although native populations of Phragmites persist, introduced invasive populations have dominated many sites and it is not clear if the two types can interbreed. This study compares patterns of differentiation in 10 microsatellite loci among North American and European Phragmites individuals with results obtained from sequencing of noncoding chloroplast DNA. Three population lineages (native, introduced and Gulf Coast) were previously identified in North America from chloroplast DNA and similar structuring was found in the nuclear genome. Each lineage was distinguished by unique alleles and allele combinations and the introduced lineage was closely related to its hypothesized source population in Europe. Size homoplasy and diagnostic base substitutions distinguishing lineages were evident at several loci, further emphasizing that native, introduced and Gulf Coast North American Phragmites lineages are genetically distinct. Gene flow between lineages was low and invasive introduced populations do not represent a hybrid population type. [source] Aripiprazole: A treatment for severe coprolalia in "refractory" Gilles de la Tourette syndromeMOVEMENT DISORDERS, Issue 3 2008Mouna Ben Djebara MD Abstract Coprolalia is one of the most distressing symptoms in Gilles de la Tourette syndrome. We report on a 28-year-old man with severe coprolalia at the forefront of symptoms, which had a dramatic impact on his social and professional life and that did not fluctuate for years. Moreover, he presented hypersensitivity to neuroleptics. The use of aripiprazole, as a last resort, induced a 75% of improvement of his symptoms with good tolerance. This suggests that aripiprazole constitutes a valuable therapeutic in coprolalia. Moreover, its biochemical class specificity makes it an alternative for patients hypersensitive to other classes of neuroleptics. © 2007 Movement Disorder Society [source] 1.8 Å structure of murine GITR ligand dimer expressed in Drosophila melanogaster S2 cellsACTA CRYSTALLOGRAPHICA SECTION D, Issue 5 2009Kausik Chattopadhyay Glucocorticoid-induced TNF receptor ligand (GITRL), a prominent member of the TNF superfamily, activates its receptor on both effector and regulatory T cells to generate critical costimulatory signals that have been implicated in a wide range of T-cell immune functions. The crystal structures of murine and human orthologs of GITRL recombinantly expressed in Escherichia coli have previously been determined. In contrast to all classical TNF structures, including the human GITRL structure, murine GITRL demonstrated a unique `strand-exchanged' dimeric organization. Such a novel assembly behavior indicated a dramatic impact on receptor activation as well as on the signaling mechanism associated with the murine GITRL costimulatory system. In this present work, the 1.8,Å resolution crystal structure of murine GITRL expressed in Drosophila melanogaster S2 cells is reported. The eukaryotic protein-expression system allows transport of the recombinant protein into the extracellular culture medium, thus maximizing the possibility of obtaining correctly folded material devoid of any folding/assembly artifacts that are often suspected with E. coli -expressed proteins. The S2 cell-expressed murine GITRL adopts an identical `strand-exchanged' dimeric structure to that observed for the E. coli -expressed protein, thus conclusively demonstrating the novel quaternary structure assembly behavior of murine GITRL. [source] At-home meat consumption in China: an empirical study,AUSTRALIAN JOURNAL OF AGRICULTURAL & RESOURCE ECONOMICS, Issue 4 2009Hongbo Liu The remarkable economic changes occurring within China since 1978 have resulted in a striking alteration in food consumption patterns, and one marked change is the increasing consumption of meat. Given China's large population, a small percentage change in per capita meat consumption could lead to a dramatic impact on the production and trade of agricultural products. Such changes have major implications for policy makers and food marketers. This paper concentrates on meat consumption patterns in the home in China. A censored linear approximate almost ideal demand system model was employed in the study, and major economic parameters were estimated for different meat items. Data used in this study were collected from two separate consumer surveys , one urban and one rural in 2005. [source] Untersuchung zur Lärmbelastung von Patienten und Pflegepersonal auf IntensivstationenBAUPHYSIK, Issue 4 2007Jörg Arnold Dipl.-Ing. Zur Untersuchung der Lärmbelastung auf Intensivstationen in Krankenhäusern wurden Schalldruckpegelmessungen sowohl in einem Patientenzimmer als auch in dem Foyer einer Intensivstation durchgeführt. Im Patientenzimmer wird eine hohe Lärmbelastung festgestellt. Zudem belegen die Messergebnisse, dass in jeder untersuchten Stunde Lärmereignisse auftraten, die leicht zum Aufwachen führen. Diese hohe Lärmdichte hat einen dramatischen Einfluss auf den Schlaf und damit auf die Erholung und Gesundung des Patienten. Es wurde eine für gesunde Patienten "sichere Aufwachschwelle" definiert, die auch in der Nacht im Mittel 5 mal je Stunde überschritten wird. Allerdings existieren in Deutschland bisher keine Grenzwerte zur Beurteilung der Lärmeinwirkung auf Patienten. Im Foyer ist in jeder untersuchten Schicht die geistige Arbeit des Pflegepersonals nach den Vorgaben der Richtlinie VDI 2058 deutlich beeinträchtigt und die zulässige Grenze für überwiegend mechanisierte Tätigkeiten wird fast erreicht. Nach dem aktuellen Arbeitsrecht sind allerdings alle Vorgaben eingehalten, da lediglich ein Grenzwert zum Schutz vor Lärmschwerhörigkeit angegeben wird. Das Ausmaß des Lärms und der dadurch verursachte Stress, sowohl für Patienten als auch für das Pflegepersonal, zeigen den dringenden Bedarf nach gesetzlichen Regelungen auch in Deutschland. Investigations on noise nuisance of patients and nursing staff. The aim of this study was to assess the exposure to noise in intensive care units. Therefore, sound pressure level measurements have been carried out in both the sickroom and the ward corridor. A high noise exposure in the sickroom was found. In addition the measurement results proves that sleep disturbing noise is occurring at all hours. This high noise density has a dramatic impact on the patients' convalescence and recovery process. One for healthy patients "certain wake-up threshold" was defined, which is exceeded 5 times per hour also in the night. There is no regulation by the German law regarding the patients' exposure to noise in hospitals. While in the ward corridor the intellectual work of the nursing staff according to VDI 2058 is disturbed significantly in every shift investigated, the permissible guideline limit for mechanical works in this specific working environment is almost achieved. But the guidelines of the current labour law are always complied with, because there is only one limit for protection against noise induced hearing loss. The magnitude of noise and stress caused thereby, for both the patients and the nursing staff, show the urgent need of specific regulations by German law. [source] Generalized MPLS-based distributed control architecture for automatically switched transport networksBELL LABS TECHNICAL JOURNAL, Issue 1 2001Yangguang Xu Current circuit-switched transport networks, such as plesiochronous digital hierarchy (PDH) and synchronous optical network/synchronous digital hierarchy (SONET/SDH), have traditionally used centralized network management for connection control. To facilitate the value-added capabilities of today's networks,such as the rapid provisioning of services, dynamic setup of bandwidth requests, and fast mesh-based restoration,distributed connection control using signaling protocols has quickly gained industry momentum. Efforts have been initiated in various standards bodies to define the automatically switched transport network (ASTN). Although many architectural choices are now available, this paper describes a distributed control plane architecture that can be applied to various circuit-switching technologies and different network applications. This architecture adopts the concept of a generalized version of multiprotocol label switching (MPLS), which extends and modifies MPLS and other protocols on the Internet to make them applicable to various transport networks and also facilitates optical data networking. Four major functional components are incorporated in this architecture: element-level resource discovery, state information dissemination, path selection, and path control modules. Using these concepts, the transport network can be viewed as a virtual nonblocking, reconfigurable backplane of different network clients. This view represents a radical departure from the traditional data networking view of transport networks as providing fixed pipes and will have a dramatic impact on future network interworking and end-to-end traffic engineering (TE). [source] Optimal nitrogen supply as a key to increased and sustained production of a monoclonal full-size antibody in BY-2 suspension culture,BIOTECHNOLOGY & BIOENGINEERING, Issue 2 2010T. Holland Abstract Plant cell cultures have been used as expression hosts for recombinant proteins for over two decades. The quality of plant cell culture-produced proteins such as full-size monoclonal antibodies has been shown to be excellent in terms of protein folding and binding activity, but the productivity and yield fell short of what was achieved using mammalian cell culture, in which the key to gram-per-liter expression levels was strain selection and medium/process optimization. We carried out an extensive media analysis and optimization for the production of the full-size human anti-HIV antibody 2G12 in N. tabacum cv. BY-2. Nitrogen source and availability was found to be one key factor for the volumetric productivity of plant cell cultures. Increased amounts of nitrate in the culture medium had a dramatic impact on protein yields, resulting in a 10,20-fold increase in product accumulation through a combination of enhanced secretion and higher stability. The results were scalable from shake flasks to stirred-tank bioreactors, where the maximum yield per cultivation volume was 8,mg,L,1 over 7 days. During the stationary phase, antibody levels were 150-fold higher in nitrogen-enriched medium compared to standard medium. The enhanced medium appeared not to affect antibody quality and activity, as determined by Western blots, surface plasmon resonance binding assays and N -glycan analysis. Biotechnol. Bioeng. 2010;107: 278,289. © 2010 Wiley Periodicals, Inc. [source] Stress response pathways in protozoan parasitesCELLULAR MICROBIOLOGY, Issue 12 2008Nathalie Vonlaufen Summary Diseases caused by protozoan parasites have a dramatic impact on world health. Emerging drug resistance and a general lack of experimental understanding has created a void in the medicine cabinet used to treat these widespread infections. A novel therapeutic idea that is receiving more attention is centred on targeting the microbe's response to the multitude of environmental stresses it encounters. Protozoan pathogens have complex life cycles, often having to transition from one host to another, or survive in a cyst form in the environment until a new host arrives. The need to respond to environmental cues and stress, and endure in less than optimal conditions, is paramount to their viability and successful progression through their life cycle. This review summarizes the research on parasitic stress responses for Apicomplexa, kinetoplastids and anaerobic protozoa, with an eye towards how these processes may be exploited therapeutically. [source] Optimizing Antiplatelet Therapy in Coronary InterventionsCLINICAL CARDIOLOGY, Issue S6 2000S. B. King III M.D. Abstract Percutaneous coronary intervention has had a dramatic impact on the current practice of cardiology. One of its important limitations, however, is the potential for producing unfavorable outcomes such as acute coronary closure following angioplasty or atherectomy or subacute thrombosis following stent implantation. These complications may lead to death, myocardial infarction, or the need for urgent bypass surgery. One mechanism underlying these clinical events is platelet-mediated thrombosis due to arterial trauma. Therapeutically, platelet activation by thromboxane and adenosine diphosphate (ADP), as well as platelet aggregation by glycoprotein IIb/IIIa (GPIIb/IIIa) receptors, has been inhibited with various pharmacologic agents. c7E3 (abciximab), a monoclonal antibody directed against GPIIb/IIIa, has been shown to have potent effects on reducing both acute and subacute complications. Other parenteral GPIIb/IIIa inhibitors, including peptide and small nonpepude molecules, have also been found to be clinically effective. Oral versions of similar drugs are currently being evaluated, but several have resulted in disappointing efficacy and safety profiles and have failed to show advantages over aspirin. With all antiplatelet agents, in particular GPIIb/IIIa receptor inhibitors, bleeding and vascular complications must be addressed. Inhibition of thromboxane-induced platelet activation with aspirin has been standard therapy for angioplasty and in the global management of vascular disease. Newer agents that block ADP-mediated platelet activation, the thienopyridines, have been found to be synergistic to aspirin in their effects on the complications of coronary intervention. Ticlopidine and, more recently, clopidogrel, in conjunction with aspirin, have become standard therapies for preventing subacute thrombosis after stent implantation. Large-scale clinical trials are ongoing to optimize their use in combination with GPIIb/IIIa inhibitors. [source] | |||||||||||||||||||||||||