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Display Approach (display + approach)

Distribution by Scientific Domains

Medical Sciences	40%

Selected Abstracts

A new Groucho TLE4 protein may regulate the repressive activity of Pax5 in human B lymphocytes

IMMUNOLOGY, Issue 4 2002
Michèle Milili
Summary During mouse B-cell development, Pax5 is an essential transcription factor that acts as an activator of B-cell-specific genes and as a repressor of alternative lineage fates. The repressive function is mediated by the recruitment of members of the Groucho co-repressor family. Using an RNA display approach, we have isolated a transcript, called QD, specifically expressed in human pro-B and pre-B cells, which is derived from the human Groucho TLE4 gene. The QD transcript contains the first four TLE4 exons and an intronic sequence 3, of exon 4, demonstrating that QD is a splice variant of TLE4. The putative resulting protein of 94 amino acids corresponds to approximately half of an N-terminal tetramerization domain. We also show specific expression of TLE4 transcripts in human B cells and of TLE4 proteins in B-cell nuclei. Moreover, we demonstrate that recombinant QD protein binds to the TLE4 Q domain and is able to abolish the TLE4/Pax5 interaction. Thus, QD could act as a negative regulator of TLE4 function, in early B-cell differentiation. [source]

Identification of a Highly Specific Hydroxyapatite-binding Peptide using Phage Display,

ADVANCED MATERIALS, Issue 10 2008
Marc D. Roy
A peptide sequence SVSVGMKPSPRP that selectively recognizes hydroxyapatite (HA) is identified by using a phage display approach. The engineered sequence exhibits chemical and structural specificity for HA over calcium carbonate and HA's amorphous calcium phosphate precursor. In situ binding to HA in a tooth cross section further demonstrates the sequence specificity and utility in nondestructive imaging applications. [source]

Porcine ESTs detected by differential display representing possible candidates for the trait ,eye muscle area'

JOURNAL OF ANIMAL BREEDING AND GENETICS, Issue 1 2000
By S. Ponsuksili
In order to identify ESTs which represent possible candidates for carcass traits in pigs, the differential display approach was used. F2 animals of a resource population and pure-bred German Landrace (DL) pigs were selected for the trait ,eye muscle area' in order to build up groups of three high and three low performing individuals within each population. To increase the probability that differentially expressed DNA fragments were not found due to the genetic background but due to differences in a few genes affecting the trait of interest, siblings were included in the high and in the low performing groups. RNA was isolated from M. longissimus dorsi and four ,intra-litter constrasting pools' were prepared: high performing F2, low performing F2, high performing DL and low performing DL. Differential display banding patterns were produced using (d)T11VA (V:A,C,G) and 20 arbitrary primers. Comparing the banding patterns of the four RNA pools revealed 27 nonshared bands. Here we report on the analysis of seven of these bands, including sequencing, search for homology and mapping using a somatic cell hybrid panel. Two clones showed high homology to known genes, two were homologous to an EST and a SINE sequence. Three clones did not show any homology. Differential expression was tested by semiquantitative reverse transcription,polymerase chain reaction (RT,PCR) and could be confirmed for six clones. [source]

Identification and characterization of novel senescence-associated genes from barley (Hordeum vulgare) primary leaves

PLANT BIOLOGY, Issue 2008
N. Ay
Abstract Leaf senescence is the final developmental stage of a leaf. The progression of barley primary leaf senescence was followed by measuring the senescence-specific decrease in chlorophyll content and photosystem II efficiency. In order to isolate novel factors involved in leaf senescence, a differential display approach with mRNA populations from young and senescing primary barley leaves was applied. In this approach, 90 senescence up-regulated cDNAs were identified. Nine of these clones were, after sequence analyses, further characterized. The senescence-associated expression was confirmed by Northern analyses or quantitative RealTime-PCR. In addition, involvement of the phytohormones ethylene and abscisic acid in regulation of these nine novel senescence-induced cDNA fragments was investigated. Two cDNA clones showed homologies to genes with a putative regulatory function. Two clones possessed high homologies to barley retroelements, and five clones may be involved in degradation or transport processes. One of these genes was further analysed. It encodes an ADP ribosylation factor 1-like protein (HvARF1) and includes sequence motifs representing a myristoylation site and four typical and well conserved ARF-like protein domains. The localization of the protein was investigated by confocal laser scanning microscopy of onion epidermal cells after particle bombardment with chimeric HvARF1-GFP constructs. Possible physiological roles of these nine novel SAGs during barley leaf senescence are discussed. [source]

Selection of D -Amino-Acid Peptides That Bind to Alzheimer's Disease Amyloid Peptide A,1,42 by Mirror Image Phage Display

CHEMBIOCHEM, Issue 8 2003
Katja Wiesehan Dr.
Abstract A mirror image phage display approach was used to identify novel and highly specific ligands for Alzheimer's disease amyloid peptide A,(1,42). A randomized 12-mer peptide library presented on M13 phages was screened for peptides with binding affinity for the mirror image of A,(1,42). After four rounds of selection and amplification the peptides were enriched with a dominating consensus sequence. The mirror image of the most representative peptide (D -pep) was shown to bind A,(1,42) with a dissociation constant in the submicromolar range. Furthermore, in brain tissue sections derived from patients that suffered from Alzheimer's disease, amyloid plaques and leptomeningeal vessels containing A, amyloid were stained specifically with a fluorescence-labeled derivative of D -pep. Fibrillar deposits derived from other amyloidosis were not labeled by D -pep. Possible applications of this novel and highly specific A, ligand in diagnosis and therapy of Alzheimer's disease are discussed. [source]