Discriminant Function Analysis (discriminant + function_analysis)

Distribution by Scientific Domains


Selected Abstracts


The use of Artificial Neural Networks to classify primate vocalizations: a pilot study on black lemurs

AMERICAN JOURNAL OF PRIMATOLOGY, Issue 4 2010
Luca Pozzi
Abstract The identification of the vocal repertoire of a species represents a crucial prerequisite for a correct interpretation of animal behavior. Artificial Neural Networks (ANNs) have been widely used in behavioral sciences, and today are considered a valuable classification tool for reducing the level of subjectivity and allowing replicable results across different studies. However, to date, no studies have applied this tool to nonhuman primate vocalizations. Here, we apply for the first time ANNs, to discriminate the vocal repertoire in a primate species, Eulemur macaco macaco. We designed an automatic procedure to extract both spectral and temporal features from signals, and performed a comparative analysis between a supervised Multilayer Perceptron and two statistical approaches commonly used in primatology (Discriminant Function Analysis and Cluster Analysis), in order to explore pros and cons of these methods in bioacoustic classification. Our results show that ANNs were able to recognize all seven vocal categories previously described (92.5,95.6%) and perform better than either statistical analysis (76.1,88.4%). The results show that ANNs can provide an effective and robust method for automatic classification also in primates, suggesting that neural models can represent a valuable tool to contribute to a better understanding of primate vocal communication. The use of neural networks to identify primate vocalizations and the further development of this approach in studying primate communication are discussed. Am. J. Primatol. 72:337,348, 2010. © 2009 Wiley-Liss, Inc. [source]


Production and perception of sex differences in vocalizations of Wied's black-tufted-ear marmosets (Callithrix kuhlii)

AMERICAN JOURNAL OF PRIMATOLOGY, Issue 4 2009
Adam S. Smith
Abstract Males and females from many species produce distinct acoustic variations of functionally identical call types. Social behavior may be primed by sex-specific variation in acoustic features of calls. We present a series of acoustic analyses and playback experiments as methods for investigating this subject. Acoustic parameters of phee calls produced by Wied's black-tufted-ear marmosets (Callithrix kuhlii) were analyzed for sex differences. Discriminant function analyses showed that calls contained sufficient acoustic variation to predict the sex of the caller. Several frequency variables differed significantly between the sexes. Natural and synthesized calls were presented to male,female pairs. Calls elicited differential behavioral responses based on the sex of the caller. Marmosets became significantly more vigilant following the playback of male phee calls (both natural and synthetic) than following female phee calls. In a second playback experiment, synthesized calls were modified by independently manipulating three parameters that were known to differ between the sexes (low-, peak-, and end-frequency). When end-frequency-modified calls were presented, responsiveness was differentiable by sex of caller but did not differ from responses to natural calls. This suggests that marmosets did not use end-frequency to determine the sex of the caller. Manipulation of peak-and low-frequency parameters eliminated the discrete behavioral responses to male and female calls. Together, these parameters may be important features that encode for the sex-specific signal. Recognition of sex by acoustic cues seems to be a multivariate process that depends on the congruency of acoustic features. Am. J. Primatol. 71:324,332, 2009. © 2008 Wiley-Liss, Inc. [source]


Long-distance dispersal and local retention of larvae as mechanisms of recruitment in an island population of a coral reef fish

AUSTRAL ECOLOGY, Issue 2 2007
HEATHER M. PATTERSON
Abstract Although recruitment of pelagic larvae is a fundamental and well-documented process in the dynamics of benthic marine populations, identifying the sources of recruitment, or the degree to which populations are connected via dispersal of larvae, has remained elusive for most marine taxa. In this study we used natural environmental markers (trace elements) recorded in fish otoliths (ear stones) as tags of natal origin. Specifically, we used the otolith core and edge chemistries of a locally endemic wrasse (Coris bulbifrons) from Lord Howe Island (LHI), Australia, and a widely distributed species (Coris picta) from three potential mainland source regions, to determine the likely sources of recruitment to C. picta populations on LHI. The use of a local endemic species, which is by definition self-recruiting, is a novel approach for ground-truthing the dispersal history of non-endemic coral reef fish. Discriminant function analyses were able to separate LHI from mainland fish, using both edge and core signatures, with a high degree of accuracy, suggesting at least some of the C. picta collected on LHI were of local origin. This result was corroborated when half of the C. bulbifrons and LHI C. picta were introduced as unknowns into a discriminant function analysis using the remaining C. bulbifrons, LHI C. picta, and the mainland C. picta as a training data set. Overall, our findings suggest that both long distance dispersal and local retention are important sources of recruitment to populations of C. picta on LHI and that otolith chemistry of endemic species could be a useful benchmark for determining the prevalence of self-recruitment in insular populations of other widespread species. [source]


Diversity of the Vocal Signals of Concave-Eared Torrent Frogs (Odorrana tormota): Evidence for Individual Signatures

ETHOLOGY, Issue 11 2009
Albert S. Feng
Male concave-eared torrent frogs (Odorrana tormota) have an unusually large call repertoire and have been shown to communicate ultrasonically. We investigated the individual specificity of male advertisement calls in order to explore the acoustic bases of individual recognition, which was demonstrated in an accompanying study. Vocalizations of 15 marked males were recorded in the field. A quantitative analysis of the signals revealed eight basic call-types. Two of them (the single- and multi-note long-calls) were investigated in more detail. Long-calls were characterized by pronounced and varying frequency modulation patterns, and abundant occurrence of nonlinear phenomena (NLP), i.e., frequency jumps, subharmonics, biphonations and deterministic chaos. The occurrence of NLP was predictable from the contour of the fundamental frequency in the harmonic segment preceding the onset of the NLP, and this prediction showed individual-specific patterns. Fifteen acoustic variables of the long calls were measured, all of which were significantly different among individuals, except biphonic segment duration. Discriminant function analysis (DFA) showed that 54.6% of the calls could be correctly assigned to individual frogs. The correct classification was above chance level, suggesting that individual specificity of calls underlie the ability of males to behaviorally discriminate the vocal signals of their neighbors from those of strangers, a remarkable feat for a frog species with a diverse vocal repertoire. The DFA classification results were lower than those for other anurans, however. We hypothesize that there is a tradeoff between an increase in the fundamental frequency of vocalizations to avoid masking by low-frequency ambient background noise, and a decrease in individual-specific vocal tract information extractable from the signal. [source]


Adherence to a daily flossing regimen in university students: effects of planning when, where, how and what to do in the face of barriers

JOURNAL OF CLINICAL PERIODONTOLOGY, Issue 9 2006
Benjamin Schüz
Abstract: Objectives: Regular (daily) dental flossing is recommended for preventing oral diseases, but adherence is unsatisfactory. Social cognitive theory (SCT) specifies determinants of dental flossing: Cognitions about risk, positive and negative outcome expectations and the perceived ability to perform behaviour predict motivation, which in turn predicts behaviour. Recent research suggests that motivation alone may not suffice to predict behaviour, and proposes if-then-planning. This study aims to predict flossing adherence from social cognitive variables and planning. Material and Methods: Questionnaire data from 157 non-dental university students on flossing, SCT variables and planning were gathered at three measurement points over 6 weeks. Residual floss was used to validate behaviour self-reports. Results: Social cognitive variables and planning correlated significantly with flossing at all times. Discriminant function analysis suggests that after controlling for Time 1 flossing, planning Time 2 (Wilk's ,=0.77; p<0.01) is more important in discriminating between adherent and non-adherent participants at Time 3 than Time 1 social cognitive measures. Regression analyses confirmed this result with planning as only predictor of flossing change (p<0.05). Conclusions: These results suggest targeting planning in interventions to increase compliance with flossing recommendations. Implications for such interventions are discussed. [source]


Sex Determination from Hand and Foot Bone Lengths,

JOURNAL OF FORENSIC SCIENCES, Issue 2 2007
D. Troy Case Ph.D.
ABSTRACT: Numerous studies have addressed sex estimation from the hands and feet with varying results. These studies have utilized multiple measurements to determine sex from the hands and feet, including measures of robusticity (e.g., base width and midshaft diameter). However, robusticity measurements are affected by activity, which can disguise underlying patterns of sexual dimorphism. The purpose of this study is to investigate the utility of length measurements of the hands and feet to estimate sex. The sample consists of white females (n=123) and males (n=136) from the Terry Collection. Discriminant function analysis was used to classify individuals by sex. The left hand outperformed both the right hand and foot producing correct classification rates exceeding 80%. Surprisingly, the phalanges were better sex discriminators than either the metacarpals or metatarsals. This study suggests that length measures are more appropriate than robusticity measures for sex estimation. [source]


Self-determination, social abilities and the quality of life of people with intellectual disability

JOURNAL OF INTELLECTUAL DISABILITY RESEARCH, Issue 11 2007
L. Nota
Summary Background The international literature has documented that self-determination is impacted by environmental factors, including living or work settings; and by intraindividual factors, including intelligence level, age, gender, social skills and adaptive behaviour. In addition, self-determination has been correlated with improved quality of life (QoL). This study sought to contribute to the growing literature base in this area by examining the relationship among and between personal characteristics, self-determination, social abilities and the environmental living situations of people with intellectual disabilities (ID). Methods The study involved 141 people with ID residing in Italy. Healthcare professionals and social workers who had known participants for at least 1 year completed measures of self-determination, QoL and social skills. Analysis of variance was conducted to verify whether different levels of intellectual impairment were associated with different degrees of the dependent variables. The Pearson product,moment correlation was used to examine any relationships among dependent variables and IQ scores. Finally, discriminant function analysis was used to examine the degree to which IQ score, age, self-determination and social abilities predicted membership in groups that were formed based on living arrangement, and on QoL status (high vs. low). Results The anova determined, as expected, that participants with more severe ID showed the lowest levels of self-determination, QoL and social abilities. Discriminant function analysis showed that (a) individuals attending day centres were distinguished from those living in institutions in that they were younger and showed greater autonomy of choice and self-determination in their daily activities; (b) basic social skills and IQ score predicted membership in the high or low QoL groups; and (c) the IQ score predicted membership in the high or low self-determination groups. A manova conducted to examine gender- and age-level differences on self-determination found gender differences; women had higher self-determination scores than men. Conclusions These findings contribute to an emerging knowledge base pertaining to the role of intraindividual and environmental factors in self-determination and QoL. In general, the study replicated findings pertaining to the relative contribution of intelligence to self-determination and QoL, added information about the potential contribution of social abilities, and pointed to the potentially important role of opportunities to make choices as a particularly important aspect of becoming more self-determined, at least in the context of residential settings. [source]


Proton magnetic resonance spectroscopic imaging to differentiate between nonneoplastic lesions and brain tumors in children,

JOURNAL OF MAGNETIC RESONANCE IMAGING, Issue 2 2006
Roula Hourani MD
Abstract Purpose To investigate whether in vivo proton magnetic resonance spectroscopic imaging (MRSI) can differentiate between 1) tumors and nonneoplastic brain lesions, and 2) high- and low-grade tumors in children. Materials and Methods Thirty-two children (20 males and 12 females, mean age = 10 ± 5 years) with primary brain lesions were evaluated retrospectively. Nineteen patients had a neuropathologically confirmed brain tumor, and 13 patients had a benign lesion. Multislice proton MRSI was performed at TE = 280 msec. Ratios of N-acetyl aspartate/choline (NAA/Cho), NAA/creatine (Cr), and Cho/Cr were evaluated in the lesion and the contralateral hemisphere. Normalized lesion peak areas (Chonorm, Crnorm, and NAAnorm) expressed relative to the contralateral hemisphere were also calculated. Discriminant function analysis was used for statistical evaluation. Results Considering all possible combinations of metabolite ratios, the best discriminant function to differentiate between nonneoplastic lesions and brain tumors was found to include only the ratio of Cho/Cr (Wilks' lambda, P = 0.012; 78.1% of original grouped cases correctly classified). The best discriminant function to differentiate between high- and low-grade tumors included the ratios of NAA/Cr and Chonorm (Wilks' lambda, P = 0.001; 89.5% of original grouped cases correctly classified). Cr levels in low-grade tumors were slightly lower than or comparable to control regions and ranged from 53% to 165% of the control values in high-grade tumors. Conclusion Proton MRSI may have a promising role in differentiating pediatric brain lesions, and an important diagnostic value, particularly for inoperable or inaccessible lesions. J. Magn. Reson. Imaging 2006. Published 2005 Wiley-Liss, Inc. [source]


DNA barcode discovers two cryptic species and two geographical radiations in the invasive drosophilid Zaprionus indianus

MOLECULAR ECOLOGY RESOURCES, Issue 3 2008
AMIR YASSIN
Abstract Comparing introduced to ancestral populations within a phylogeographical context is crucial in any study aiming to understand the ecological genetics of an invasive species. Zaprionus indianus is a cosmopolitan drosophilid that has recently succeeded to expand its geographical range upon three continents (Africa, Asia and the Americas). We studied the distribution of mitochondrial DNA (mtDNA) haplotypes for two genes (CO-I and CO-II) among 23 geographical populations. mtDNA revealed the presence of two well-supported phylogenetic lineages (phylads), with bootstrap value of 100%. Phylad I included three African populations, reinforcing the African-origin hypothesis of the species. Within phylad II, a distinct phylogeographical pattern was discovered: Atlantic populations (from the Americas and Madeira) were closer to the ancestral African populations than to Eastern ones (from Madagascar, Middle East and India). This means that during its passage from endemism to cosmopolitanism, Z. indianus exhibited two independent radiations, the older (the Eastern) to the East, and the younger (the Atlantic) to the West. Discriminant function analysis using 13 morphometrical characters was also able to discriminate between the two molecular phylads (93.34 ± 1.67%), although detailed morphological analysis of male genitalia using scanning electron microscopy showed no significant differences. Finally, crossing experiments revealed the presence of reproductive barrier between populations from the two phylads, and further between populations within phylad I. Hence, a bona species status was assigned to two new, cryptic species: Zaprionus africanus and Zaprionus gabonicus, and both were encompassed along with Z. indianus and Zaprionus megalorchis into the indianus complex. The ecology of these two species reveals that they are forest dwellers, which explains their restricted endemic distribution, in contrast to their relative cosmopolitan Z. indianus, known to be a human-commensal. Our results reconfirm the great utility of mtDNA at both inter- and intraspecific analyses within the frame of an integrated taxonomical project. [source]


Can the Neuropathic Pain Scale Discriminate Between Non-neuropathic and Neuropathic Pain?

PAIN MEDICINE, Issue 2 2008
David A. Fishbain MD, FAPA
ABSTRACT Objectives., 1) To determine if the neuropathic pain scale (NPS) can be used to classify chronic pain patients (CPPs) as having primarily neuropathic vs non-neuropathic pain, and furthermore; 2) to determine what, if any, cut-off score can be used to reliably make this determination. Design., A total of 305 CPPs consecutive admissions to The Rosomoff Pain Center were administered the NPS and were assigned a diagnosis according to the physical examination and all available test results. CPPs with a diagnosis of chronic radiculopathy and spondylolysis/degenerative arthritis were segregated into two groups for the purposes of having a group representative of neuropathic pain (chronic radiculopathy) and non-neuropathic pain (spondylolysis/degenerative arthritis). Applying neuropathic pain criteria to each "of these two groups": a neuropathic pain "subtype" was identified within the chronic radiculopathy group; and, a non-neuropathic pain "subtype" was identified within the spondylolysis/degenerative arthritis group. This step was performed in order to assure that the CPPs selected for further analysis were truly representative of neuropathic and non-neuropathic pain. Discriminant function analysis was then employed to determine if NPS scoring could differentiate between these two "subtypes." Results from the discriminant function analysis model were utilized to derive an NPS cut-off score above which CPPs would be classified as having neuropathic pain. For the diagnoses of myofascial pain syndromes, spinal stenosis, epidural fibrosis, fibromyalgia, complex regional pain syndromes 1 and 2, and failed back surgery syndrome, a predicted NPS score was calculated and compared with the cut-off score. Setting., Multidisciplinary pain facility. Patients., Chronic pain patients. Results., The NPS appeared to be able to separate CPPs into neuropathic pain vs non-neuropathic pain subtypes. The derived cut-off score from the model was 5.53. Myofascial pain syndrome and spinal stenosis had predictive scores lower than this cut-off score at 3.81 and 4.26, respectively. Epidural fibrosis, fibromyalgia, complex regional pain syndromes 1 and 2, and failed back surgery syndrome had predictive scores higher than the cut-off score at 6.15, 6.35, 6.87, 9.34, and 7.19, respectively. Conclusions., The NPS appears to be able to discriminate between neuropathic and non-neuropathic pain. A debate is currently raging as to whether diagnoses, such as fibromyalgia and complex regional pain syndrome 1, can be classified as neuropathic. Our NPS cut-off score results suggest that these diagnoses may have a neuropathic pain component. The reliability and validity of our NPS method will need to be tested further in other neuropathic pain models, such as diabetic peripheral neuropathic pain. [source]


Determination of sex from the hyoid bone

AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, Issue 2 2010
Sarah C. Kindschuh
Abstract This article explores size differences related to sex in the hyoid bones from the Robert J. Terry Anatomical Collection. A series of measurements were taken from 398 hyoids, both fused and unfused. The inclusion of unfused hyoids in the study provides the opportunity to investigate previously unknown size differences between sexes as well as to determine their utility in determining sex. Two-way ANOVA was used to explore differences in hyoid size as related to ancestry and sex. Discriminant function analysis was employed to test the ability of the hyoids to be classified by sex. Six discriminant function equations ranging in accuracy from 82% to 85% are provided, each of which is more accurate than many of the discriminant functions developed in past hyoid research, are simple to use, and can be used to estimate the sex of a hyoid regardless of its state of fusion. In addition to providing further information about the morphological form of the hyoid, these analyses provide a method that can be easily employed to assess sex of the individual from the hyoid bone. Am J Phys Anthropol 143:279,284, 2010. © 2010 Wiley-Liss, Inc. [source]


Sex determination from the occipital condyle: Discriminant function analysis in an Eighteenth and Nineteenth Century British sample

AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, Issue 4 2009
René Gapert
Abstract Fragmentary human remains compromised by different types of inhumation, or physical insults such as explosions, fires, and mutilations may frustrate the use of traditional morphognostic sex determination methods. The basicranium is protected by a large soft tissue mass comprising muscle, tendon, and ligaments. As such, the occipital region may prove useful for sex identification in cases of significantly fragmented remains. The aims of this paper are to (1) evaluate sexual dimorphism in British cranial bases by manually recorded unilateral and bilateral condylar length and width as well as intercondylar measurements and (2) develop discriminant functions for sex determination for this cranial sample. The crania selected for this study are part of the 18th,19th century documented skeletal collection of St. Bride's Church, Fleet Street, London. Adult human skulls (n = 146; ,75/,71) were measured to derive statistical functions. Results indicated that expression of sexual dimorphism in the occipital condylar region within the St. Bride's population is demonstrable but low. Crossvalidated classification accuracy ranged between 69.2 and 76.7%, and sex bias ranged from 0.3 to 9.7%. Therefore, the use of discriminant functions derived from occipital condyles, especially in British skeletal populations, should only be considered in cases of fragmented cranial bases when no other morphognostic or morphometric method can be utilized for sex determination. Am J Phys Anthropol, 2009. © 2008 Wiley-Liss, Inc. [source]


Dialects in pygmy marmosets?

AMERICAN JOURNAL OF PRIMATOLOGY, Issue 4 2009
Population variation in call structure
Abstract Population variation in primate vocal structure has been rarely observed. Here, we report significant population differences in the structure of two vocalizations in wild pygmy marmosets (Trills and J calls). We studied 14 groups of pygmy marmosets Callithrix (Cebuella) pygmaea pygmaea from five populations in northeastern Ecuador. We analyzed the acoustic structure of Trills and J calls recorded from two adult animals in each group through focal samples. Although individuals and groups within a population differed in call structure, we found consistent structural differences at a population level for Trills and J calls. Pair-wise comparisons for the two call types point to San Pablo and Amazoonico as the populations that differed the most, whereas Hormiga and Zancudococha showed the least differences. Discriminant function analysis indicates that calls from each population could be classified accurately at rates significantly above chance. Habitat acoustics, social factors and genetic drift may explain interpopulation vocal differences. This is the first evidence of within-subspecies vocal differences, or dialects, in wild populations of a neotropical primate species. Am. J. Primatol. 71:333,342, 2009. © 2009 Wiley-Liss, Inc. [source]


Do physical activity levels influence the cardiovascular-related physiological characteristics of postmenopausal women?

AUSTRALASIAN JOURNAL ON AGEING, Issue 4 2009
Fiona Barnett
Aim:, This study aimed to determine whether cardiovascular-related physiological differences existed among postmenopausal women in relation to their physical activity levels. Methods:, Participants were postmenopausal women (n= 101) resident in North Queensland. A self-report questionnaire determined recent exercise history. Anthropometric and physiological measures were obtained. Participants also performed a six-minute graded exercise test to determine cardiorespiratory fitness. Results:, Compared with the women who exercised, those women who did not exercise had a lower level of cardiorespiratory fitness (P= 0.00) and higher resting diastolic blood pressure (P= 0.01), BMI (P= 0.00) and WHR (P= 0.02). Discriminant function analysis found that a combination of BMI and cardiorespiratory fitness discriminated between the two groups. Conclusions:, Postmenopausal women who performed moderate-intensity physical activity had more favourable cardiovascular-related physiological characteristics. Health professionals should encourage more postmenopausal women to participate in moderate-intensity activity to reduce the risk of cardiovascular disease. [source]


Large cava septi pellucidi in schizophrenic patients, alcoholics, head-traumatized, and normal individuals: morpholgical features and forensic implications.

ACTA NEUROPSYCHIATRICA, Issue 1 2006
A postmortem study
Background:, The enlarged cava septi pellucidi (CSP = 6 mm in length) have been reported as a reliable marker of an underlying neuropsychiatric disease or disorder. Differences in the dimensions of cava longer than 6 mm associated with a neuropsychiatric impairment could be of possible clinical and forensic significance. Methods:, We obtained 479 brains from autopsied persons (310 males and 169 females, aged 22,89 years) and observed that 110 brains (75 males and 35 females) had CSP, of which the length of CSP was equal to or longer than 6 mm on 69 (49 males and 20 females) of them. These cava were classified into four groups depending on the past medical histories of the autopsied person: five without neuropsychiatric history (asymptomatic CSP), 25 schizophrenic patients, 22 alcoholics, and 17 with a past head trauma (symptomatic CSP). Results:, The linear parameters of CSP (i.e. length, width) of the symptomatic and asymptomatic groups were measured and were statistically analyzed. Analysis revealed that the cava in the group of schizophrenic patients were significantly longer and wider. Conclusions:, Discriminant function analysis was used to derive a mathematical formula to classify CSP into one of the groups obtained based on width measurements of the cavum. [source]


Acoustic Features of Female Chacma Baboon Barks

ETHOLOGY, Issue 1 2001
Julia Fischer
We studied variation in the loud barks of free-ranging female chacma baboons (Papio cynocephalus ursinus) with respect to context, predator type, and individuality over an 18-month period in the Moremi Game Reserve, Botswana. To examine acoustic differences in relation to these variables, we extracted a suite of acoustic parameters from digitized calls and applied discriminant function analyses. The barks constitute a graded continuum, ranging from a tonal, harmonically rich call into a call with a more noisy, harsh structure. Tonal barks are typically given when the signaler is at risk of losing contact with the group or when a mother and infant have become separated (contact barks). The harsher variants are given in response to large predators (alarm barks). However, there are also intermediate forms between the two subtypes which may occur in both situations. This finding is not due to an overlap of individuals' distinct distributions but can be replicated within individuals. Within the alarm bark category, there are significant differences between calls given in response to mammalian carnivores and those given in response to crocodiles. Again, there are intermediate variants. Both alarm call types are equally different from contact barks, indicating that the calls vary along different dimensions. Finally, there are consistent, significant differences among different individuals' calls. However, individual identity in one call type cannot directly be inferred from knowledge of the individuals' call characteristics in the other. In sum, the barks of female baboons potentially provide rich information to the recipients of these signals. The extent to which baboons discriminate between alarm and contact barks, and classify calls according to context and/or acoustic similarity will be described in a subsequent paper. [source]


VARIATION OF SHELL SHAPE IN THE CLONAL SNAIL MELANOIDES TUBERCULATA AND ITS CONSEQUENCES FOR THE INTERPRETATION OF FOSSIL SERIES

EVOLUTION, Issue 2 2000
Sarah Samadi
Abstract., Interpreting paleontological data is difficult because the genetic nature of observed morphological variation is generally unknown. Indeed, it is hardly possible to distinguish among several sources of morphological variation including phenotypic plasticity, sexual dimorphism, within-species genetic variation or differences among species. This can be addressed using fossil organisms with recent representatives. The freshwater snail Melanoides tuberculata ranks in this category. A fossil series of this and other species have been studied in the Turkana Basin (Kenya) and is presented as one of the best examples illustrating the punctuated pattern of evolution by the tenants of this theory. Melanoides tuberculata today occupies most of the tropics. We studied variation of shell shape in natural populations of this parthenogenetic snail using Raup's model of shell coiling. We considered different sources of variation on estimates of three relevant parameters of Raup's model: (1) variation in shell shape was detected among clones, and had both genetic and environmental bases; (2) sexual dimorphism, in those clones in which males occur, appeared as an additional source of shell variation; and (3) ecophenotypic variation was detected by comparing samples from different sites and years within two clones. We then tested the performance of discriminant function analyses, a classical tool in paleontological studies, using several datasets. Although the three sources of variation cited above contributed significantly to the observed morphological variance, they could not be detected without a priori knowledge of the biological entities studied. However, it was possible to distinguish between M. tuberculata and a related thiarid species using these analyses. Overall, this suggests that the tools classically used in paleontological studies are poorly efficient when distinguishing between important sources of within-species variation. Our study also gives some empirical bases to the doubts cast on the interpretation of the molluscan series of the Turkana Basin. [source]


Attachment in low-SES rural Appalachian infants: Contextual, infant, and maternal interaction risk and protective factors

INFANT MENTAL HEALTH JOURNAL, Issue 6 2001
Margaret Fish
Attachment classifications were obtained for 95 low-socioeconomic-status (SES) rural Appalachian infants in the Strange Situation procedure at 15 months. The distribution of secure (B) and insecure (A, C, D) infants was similar to other low-SES samples and significantly different from low-risk samples. Levels of contextual and infant risk, together with maternal responsiveness to crying and pattern of sensitivity from 4 to 9 months, predicted attachment security. High social support, when examined as a protective factor, related to reduced contextual risk, but not to increased likelihood of security. Exploratory discriminant function analyses showed that infants in secure relationships differed in positive directions on contextual and maternal interactional factors. Insecure-organized (A and C) infants experienced contextual and maternal interaction risks, while insecure-disorganized (D) infants were best distinguished by infant characteristics, including greater likelihood of being male and low use of mother as a secure base at 9 months. ©2001 Michigan Association for Infant Mental Health. [source]


Bizygomatic breadth determination in damaged skulls

INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, Issue 5 2010
C. L. Oskam
Abstract Metric and discriminant function analyses of the skull have been used successfully to determine ancestry and sex from human skeletal remains in both forensic and archaeological contexts. However, skulls are frequently discovered in damaged condition. One structure that is commonly fragmented, even when the rest of the skull is preserved, is the zygomatic arch. The bizygomatic width is an important measurement in craniometry and in forensic facial reconstruction for determining facial width; therefore we propose a simple linear regression model to predict the bizygomatic width of skulls with damaged zygomatic arches. Thirty-one adult skulls originating from the Indian sub-continent were used to measure the bizygomatic width. Then, on the same skulls, a straight steel wire was placed at the superior surface of the temporal and zygomatic origins of the zygomatic arch to simulate the zygomatic arch reconstruction on damaged skulls. These wire measurements were used to fit a simple linear regression model between the bizygomatic widths and the wire measurements, and the estimated regression model; Bizygomatic Width (bone),=,0.61,+,1.02(wire measurement), has a very high R2 value of 0.91. Hence, this model could effectively be used to predict bizygomatic widths based on wire measurements. In addition, the bizygomatic widths and wire measurements were collected from 14 New Zealand European skulls to test the ability of the regression model to determine bizygomatic widths from different ethnic groups. This model accurately predicted the bizygomatic widths in New Zealands of European origin skulls and therefore suggests that this regression model could be used for other ethnic groups. The importance of the bizygomatic width for craniometric analysis makes this regression model particularly useful for analysing archaeological samples. Furthermore, this regression line can be used in the field of forensic facial reconstruction to reconstruct damaged zygomatic arches prior to facial reconstructions. Copyright © 2009 John Wiley & Sons, Ltd. [source]


Hydrological disturbance benefits a native fish at the expense of an exotic fish

JOURNAL OF APPLIED ECOLOGY, Issue 5 2006
F. LEPRIEUR
Summary 1Some native fish in New Zealand do not coexist with introduced salmonids. Previous studies of disjunct distributions of exotic brown trout Salmo trutta and native galaxiids demonstrated native extirpation except where major waterfalls prevented upstream migration of trout. In the Manuherikia River system, we predicted that water abstraction might be a further factor controlling the spatial distribution of both the invader and a native fish. 2We applied multiple discriminant function analyses to test for differences in environmental conditions (catchment and instream scales) at sites with roundhead galaxias Galaxias anomalus and brown trout in sympatry and allopatry. We then used a supervised artificial neural network (ANN) to predict the presence,absence of G. anomalus and brown trout (135 sites). The quantification of contributions of environmental variables to ANN models allowed us to identify factors controlling their spatial distribution. 3Brown trout can reach most locations in the Manuherikia catchment, and often occur upstream of G. anomalus. Their largely disjunct distributions in this river are mediated by water abstraction for irrigation, together with pool habitat availability and valley slope. Trout are more susceptible than the native fish to stresses associated with low flows, and seem to be prevented from eliminating galaxiid populations from sites in low gradient streams where there is a high level of water abstraction. 4Synthesis and applications. In contrast to many reports in the literature, our results show that hydrological disturbance associated with human activities benefits a native fish at the expense of an exotic in the Manuherikia River, New Zealand. Water abstraction is also known to have negative impacts on native galaxiids, therefore we recommend restoring natural low flows to maintain sustainable habitats for native galaxiids, implementing artificial barriers in selected tributaries to limit trout predation on native fish, and removing trout upstream. [source]


Squirrel monkey chuck call: vocal response to playback chucks based on acoustic structure and affiliative relationship with the caller

AMERICAN JOURNAL OF PRIMATOLOGY, Issue 3 2002
Joseph Soltis
Abstract Adult female squirrel monkeys (genus Saimiri) that are socially familiar often exchange the chuck vocalization, which differs acoustically across individuals. We used behavioral observations, vocalization playback experiments, and analysis of the acoustic properties of vocalizations to investigate the effect of caller identity and acoustic structure on vocal response to playback chucks in two all-female social groups (n=10 females). Females were most likely to respond with a chuck to the playback chucks of their closely affiliated partners compared to those of nonaffiliated group members. This shows for the first time that the chuck stimulus alone is sufficient to elicit a chuck response from a female's affiliated partner. Additionally, females responded with a chuck mostly to familiar playback chucks from their own group and least to playbacks of silent controls. Unfamiliar playback chucks from the same species and a different squirrel monkey species elicited chuck responses intermediate between familiar chucks and silent controls. Post-hoc discriminant function analyses provide preliminary evidence that females are most likely to respond to unfamiliar chucks when those chucks are close in acoustic structure to familiar chucks from their own social group. These results provide a provisional explanation for error in the squirrel monkey signal processing system, in which unfamiliar chucks never heard before nevertheless elicit a chuck response if they are similar in acoustic structure to familiar group chucks. © 2002 Wiley-Liss, Inc. [source]


Sex differences in juvenile rhesus macaque (Macaca mulatta) agonistic screams: Life history differences and effects of prenatal androgens

DEVELOPMENTAL PSYCHOBIOLOGY, Issue 4 2005
Michelle L. Tomaszycki
Abstract This study investigated sex differences in juvenile rhesus macaque (Macaca mulatta) vocal behavior during agonistic contexts, and the effects of prenatal androgens on these differences. A total of 59 subjects (5,8 per treatment group) received exogenous androgen (testosterone enanthate), an anti-androgen (flutamide) or vehicle injections (DMSO) for 30 or 35 days during the second (early) or third (late) trimester of pregnancy. An additional 19 unmanipulated controls were included in the analysis. Screams by juvenile males and females between the ages of 1 and 3 years were compared to the screams of adult female exemplars using a discriminant function analysis. Juvenile females produced more adult-female like screams than did juvenile males. Females exposed to androgen treatment late in gestation produced a more masculine pattern of screams. Flutamide treatment in males either early or late in gestation did not significantly affect scream production. Flutamide treatments in females late in gestation, however, masculinized scream production. Androgen treatments administered late in gestation hyper-masculinized male scream production. No sex differences in the contextual usage of screams emerged. These findings suggest that both life history differences and the early hormone environment contribute to sex differences in juvenile rhesus macaque vocal production. © 2005 Wiley Periodicals, Inc. Dev Psychobiol 47: 318,327, 2005. [source]


Host-related life history traits in interspecific hybrids of cactophilic Drosophila

ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, Issue 1 2008
E. M. Soto
Abstract In the genus Drosophila (Diptera: Drosophilidae), interspecific hybridization is a rare phenomenon. However, recent evidence suggests a certain degree of introgression between the cactophilic siblings Drosophila buzzatii Patterson & Wheeler and Drosophila koepferae Fontdevila & Wasserman. In this article, we analyzed larval viability and developmental time of hybrids between males of D. buzzatii and females of D. koepferae, raised in media prepared with fermenting tissues of natural host plants that these species utilize in nature as breeding sites. In all cases, developmental time and larval viability in hybrids was not significantly different from parental lines and, depending on the cross, hybrids developed faster than both parental species or than the slowest species. When data of wing length were included in a discriminant function analysis, we observed that both species can be clearly differentiated, while hybrids fell in two categories, one intermediate between parental species and the other consisting of extreme phenotypes. Thus, our results point out that hybrid fitness, as measured by developmental time and viability, is not lower than in the parental species. [source]


Individual Acoustic Variation in Fallow Deer (Dama dama) Common and Harsh Groans: A Source-Filter Theory Perspective

ETHOLOGY, Issue 3 2007
Elisabetta Vannoni
Mammals are able to distinguish conspecifics based on vocal cues, and the acoustic structure of mammal vocalizations is directly affected by the anatomy and action of the vocal apparatus. However, most studies investigating individual patterns in acoustic signals do not consider a vocal production-based perspective. In this study, we used the source-filter model of vocal production as a basis for investigating the acoustic variability of fallow deer groans. Using this approach, we quantified the potential of each acoustic component to carry information about individual identity. We also investigated if cues to individual identity carry over among the two groan types we describe: common and harsh groans. Using discriminant function analysis, we found that variables related to the fundamental frequency contour and the minimum frequencies of the highest formants contributed most to the identification of a given common groan. Common groans were individually distinctive with 36.6% (53.6% with stepwise procedure) of groans assigned to the correct individual. This level of discrimination is approximately six times higher than that predicted by chance. In addition, univariate anovas showed significant inter-individual variation in the minimum formant frequencies when common and harsh groans were combined, suggesting that some information about individuality is shared between groan types. Our results suggest that the sound source and the vocal tract resonances act together to determine groan individuality and that enough variation exists to potentially allow individual recognition based on groans. [source]


Metabolic fingerprinting allows discrimination between Ulmus pumila and U. minor, and between U. minor clones of different susceptibility to Dutch elm disease

FOREST PATHOLOGY, Issue 4 2008
J. A. Martin
Summary Experiments were conducted to test whether Fourier transform-infrared (FT-IR) spectroscopy, coupled with chemometric methods, can distinguish healthy xylem tissues collected from elms known to differ significantly in their susceptibility to Ophiostoma novo-ulmi Brasier. Twig samples from resistant Ulmus pumila L., susceptible U. minor Mill. and resistant U. minor clones were harvested on 1 May, 15 May, 1 June, 1 July and 1 September 2004, and subjected to FT-IR analysis. The application of principal component analysis to the spectral data, followed by discriminant function analysis, discriminated between the three groups of samples in all harvesting dates. The examination of the DF-loading plots allowed the identification of key regions within the spectra for the separation between clusters. The chemical assignments of these key regions allowed the following interpretations to be made: (i) U. pumila tissues contained enhanced levels of starch, cellulose and lignin with respect to U. minor tissues and (ii) resistant U. minor tissues contained enhanced levels of starch, cellulose and pectic polysaccharides with respect to susceptible U. minor tissues. The possible roles of the compositional differences in disease resistance, as well as the potential use of FT-IR spectroscopy and chemometrics as a tool for screening resistant elms are discussed. [source]


Distinguishing between the nests of sympatric chimpanzees and gorillas

JOURNAL OF APPLIED ECOLOGY, Issue 2 2007
CRICKETTE SANZ
Summary 1Our current inability to estimate precisely the population sizes of chimpanzees and gorillas across much of the Congo Basin has been detrimental to the development of conservation strategies for the preservation of these endangered apes. Systematic counts of nests are currently the most commonly used method to estimate ape abundance, but distinguishing between the nests of sympatric chimpanzees and gorillas has proven to be an enduring obstacle to estimating species-specific abundance. In general, the builder of more than 75% of nests recorded during surveys is undetermined. We hypothesized that sleeping habits and nest building patterns would allow us to differentiate between the nests of these apes. 2We constructed a predictive model using stepwise discriminant function analysis to determine characteristics that accurately distinguished between chimpanzee and gorilla nests. We analysed 13 variables associated with 3425 ape nests from three independent surveys conducted in the Goualougo Triangle of the Nouabalé-Ndoki National Park, Republic of Congo. 3The model correctly classified more than 90% of nests in our validation subsample. Nest height, nest type, forest type and understorey closure were identified as important variables for distinguishing between chimpanzee and gorilla nests at this site. Attributing nests to either species increased the precision of resulting density estimates, which enhanced the statistical power to detect trends in population fluctuation. 4Although specific variables may differ between study sites, we have demonstrated that predictive models to distinguish between the nests of sympatric chimpanzee and gorillas provide a promising approach to improving the quality of ape survey data. 5Synthesis and applications. Our study introduces an innovative solution to the dilemma of discriminating between the nests of sympatric chimpanzees and gorillas, which increases the specificity and precision of resulting ape abundance estimates. There is an urgent need to improve methods to evaluate and monitor remaining ape populations across western and central Africa that are experiencing the imminent threats of emergent diseases, poaching and expanding human development. Increasing the quality of density estimates from field survey data will aid in the development of local conservation initiatives, national strategies and international policies on behalf of remaining ape populations. [source]


Continuous, age-related plumage variation in male Kirtland's Warblers

JOURNAL OF FIELD ORNITHOLOGY, Issue 1 2007
John R. Probst
ABSTRACT The ability to age individual birds visually in the field based on plumage variation could provide important demographic and biogeographical information. We describe an approach to infer ages from a distribution of plumage scores of free-ranging male Kirtland's Warblers (Dendroica kirtlandii). We assigned ages to males using a scoring scheme (0,12 points) based on variation in plumage coloration, brightness, and contrast on three dorsal and three ventral body regions presumed to be age-related. The distribution of total additive plumage scores for 875 breeding males was normally distributed, indicating no distinct age classes. Thus, we developed provisional plumage-age classes of second year (SY) and after second-year (ASY), and compared them to the total plumage scores of a smaller subsample of known age (N= 92) and minimum age (N= 143) males. Plumage scores of known-age male Kirtland's Warblers increased nonlinearly with age (rs= 0.67), but with some overlap. The median plumage score for SY males (median = 5.0) was significantly lower than for third-year (TY) males (median = 7.0) and after third-year (3 year and older) males (median = 8.0), indicating that the plumage of male Kirtland's Warblers becomes more distinctive and brighter with age. Linear discriminant function analysis differentiated ASY male Kirtland's Warbler from SY males with 78.3% accuracy. Investigators could use the distribution of plumage scores and approximate age structures to document changes in male age structure during colonization, use, and abandonment of habitats by Kirtland's Warblers or other species that occupy early successional habitats. Aging free-ranging birds based on a plumage scoring scheme may be especially critical for demographic studies of less-studied species where it is unlikely that a banding program will be initiated, but where plumage-age inferences or management decisions must be made. SINOPSIS Describimos una forma para inferir la edad a través de una distribución de marcadores en machos silvestres de la reinita Dendroica kirtlandii. Utilizando dicho acercamiento, asignamos edad relativa a machos basándonos en un esquema de puntuación (0,12) para la coloración del plumaje, su brillantez, y contraste en tres localidades del dorso y la parte ventral, que han sido relacionadas con la edad de estos. La distribución de las puntuaciones de 875 machos fue normal, lo que indica que no hubo forma de distinguir las diferentes edades. Por ende, desarrollamos un divisiones de clases, basándonos en diferencias en el plumaje para individuos de segundo año (SA) y posterior al segundo año (PSA) y los comparamos a las puntuaciones de una pequeña muestra de aves (N= 92) cuya edad era conocida y a otra en que sabíamos la edad mínima (N= 143). La puntuación total para individuos de edad conocida aumento de forma no-lineal con la edad (Rs = 0.67), con un pequeño solapamiento en el plumaje total. La puntuación para machos de segundo año (SA) (mediana = 5.0) fue significativamente menor que para aves de tercer año (mediana = 7.0) y esta a su vez que para aves mayores a tres años (mediana = 8.0), lo que indica que el plumaje de los machos, en las aves estudiadas, se torna más brillante con la edad. Un análisis lineal de función discriminativa pudo diferenciar entre aves PSA y SA con un 78.3% de exactitud. Los investigadores han utilizado estas diferencias en el plumaje para clasificar entre los grupos machos territoriales que no han sido anillados. También pudieran utilizar estas particularidades y diferencias en el plumaje para aproximar la estructura de edades entre machos silvestres y hacer inferencias sobre la demografía y reclutamiento de aves en habitats de diferente calidad. [source]


Sexual dimorphism and dichromatism in Steere's Liocichla (Liocichla steerii)

JOURNAL OF FIELD ORNITHOLOGY, Issue 4 2006
Herman L. Mays Jr
ABSTRACT Although sexual differences in birds can be extreme, differences between males and females in body size and plumage color are more subtle in many species. We used a genetic-based approach to determine the sex of male and female Steere's Liocichla (Liocichla steerii) and examine the degree of size dimorphism and plumage dichromatism in this apparently monomorphic species. We found that males were significantly larger than females. In addition, Steere's Liocichla have a prominent yellow plumage patch on the lores that was significantly larger in males than females for both live birds and museum specimens. We also used reflectance spectrometry to quantify the color of the yellow-green breast feathers of Steere's Liocichla and found no significant differences between males and females in brightness, intensity, saturation, or hue. However, females tended to have brighter breast plumage, particularly at long wavelengths. Collectively, these color variables were useful in discriminating birds according to sex when used in a discriminant function analysis. Our study suggests that sexual selection may be more widespread than once assumed, even among birds considered monomorphic, and emphasizes the need for additional data from tropical and subtropical species. SINOPSIS Aunque las diferencias sexuales entre algunas especies puede ser estrechas, un muchas de estas las diferencias corporales entre los sexos pudiera ser sutiles. Abordamos el asunto basados en genética para determinar el sexo en Liocichla steerii y examinamos el grado de dimorfismo sexual y dicromatismo en el plumaje en esta especie la cual aparentemente es monomórfica. Encontramos que los machos eran significativamente de mayor tamaño que las hembras. Además encontramos en los machos un parcho amarillo en los lores significativamente más grande que en las hembras, tanto en aves del estado silvestre como en pieles de museos. Usamos espectrometría para cuantificar el color de las plumas verde-amarillentas del pecho de las aves y no encontramos diferencias significativas entre los sexos, con respecto a la brillantez, insensidad, saturación o matiz color. Sin embargo, las hembras tuvieron la tendencia a tener el plumaje más brillante en el pecho particularmente en el espectro de onda larga. Colectivamente, estas variables de color fueron útiles para discriminar entre el sexo de las aves, particularmente cuando fueron examinadas utilizando un análisis discriminante de funciones. Nuestro estudio sugiere que la selección sexual pudiera estar más ampliamente distribuida que lo previamente asumido, aún en aves consideradas monomórficas. Además el trabajo enfatiza la necesidad de llevar a cabo estudios adicionales, de este tipo, tanto en especies del trópico como de la zona templada. [source]


Sex determination of Red-tailed Hawks (Buteo jamaicensis calurus) using DNA analysis and morphometrics

JOURNAL OF FIELD ORNITHOLOGY, Issue 1 2006
Kara C. Donohue
ABSTRACT Currently, the sex of Red-tailed hawks (Buteo jamaicensis) cannot be determined by in-hand methods. Males and females do not differ in plumage and overlap in size. We collected feather samples and morphological measurements from migrating birds at four sites in the western United States. Sex was determined for individual birds using sex-specific DNA markers and polymerase chain reaction was used to identify these DNA markers. Using discriminant function analysis, we created equations for determining the sex of Red-tailed Hawks using in-hand measurements based on the DNA-determined sexes. We formed two equations, one for adults that was 98% accurate, and one for hatch-year birds that was 97% accurate. The ability to determine the sex of western Red-tailed Hawks using morphological measurements will be useful to investigators examining possible intra- or intersexual differences. SINOPSIS Actualmente no se puede determinar el sexo de Buteo jamaicensis, aun teniendo al ave en la mano. Durante el periodo migratorio tomamos medidas morfométricas y coleccionamos plumas de estas aves en cuatro localidades de la parte oeste de los Estados Unidos. Determinamos el sexo de los individuos utilizando marcadores específicos de ADN para el sexo y reacciones en cadena de polimerasa para identificar los marcadores de ADN. Una vez determinado el sexo por medio de ADN, se hizo un análisis de función discriminativa de los datos para producir una ecuación que permitiera identificar el sexo de las medidas morfométricas de individuos capturados. Se produjeron dos ecuaciones, una para los adultos y otra para jóvenes de un año con 98 y 97% de exactitud, respectivamente. La capacidad para determinar el sexo en la especie, utilizando morfometría será de gran utilidad para investigadores interesados en determinar posibles diferencias intra e intersexuales. [source]


Microbial and Sensory Assessment of Milk with an Electronic Nose

JOURNAL OF FOOD SCIENCE, Issue 2 2002
F. Korel
ABSTRACT: An electronic nose (e-nose) was used to assess milk odor inoculated with Pseudomonas fluorescens or Bacillus coagulans, and odors were correlated with microbial loads and sensory scores. Sterile whole, reduced-fat, and fat-free milk were inoculated, stored at 1.7, 7.2, and 12.8 °C, and evaluated at d 0, 3, 5, 7, and 10 by e-nose and sensory panel. Aerobic plate counts were performed. E-nose readings, microbial counts, and sensory data were analyzed using discriminant function analysis. The e-nose discriminated differences in odor due to microbial load and sensory data. This may lead to a rapid method for determining sensory evaluation and microbial loads of milk. [source]