Different Requirements (different + requirement)

Distribution by Scientific Domains


Selected Abstracts


The Space Mission MIR'97: operational aspects

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, Issue 12 2000
R. Ewald
Background A German astronaut visited the MIR space station between 10 February and 2 March 1997. Together with his Russian colleagues, he conducted a series of scientific investigations before, during and after his stay aboard the MIR station. Research performed during this flight was part of a global space life sciences programme and focused on metabolic homeostasis, fluid balance, calcium homeostasis and cardiovascular regulatory mechanisms. The main goal of the scientific experiments was to use this mission as a milestone to establish international networks of scientific collaboration using space research as a tool for focused research in respective fields. Thus, in most cases the results obtained from the astronaut complemented a series of results obtained on ground and from other flights. In other cases, they extended previous results and opened new fields for future research. Participants Human space flight with astronauts serving as operators and at the same time as test subjects is very complex. Many people, including mission control, a science management team, medical operations, ethics committees and a medical board, participated to harmonize the different requirements, thus making a maximal scientific outcome possible. Conclusion In summary, this space mission may be seen as a model for focused long-term multidisciplinary international research, and demonstrates that space medicine is no longer adventure but science. [source]


Analysis of DNA-binding sites on Mhr1, a yeast mitochondrial ATP-independent homologous pairing protein

FEBS JOURNAL, Issue 6 2010
Tokiha Masuda
The Mhr1 protein is necessary for mtDNA homologous recombination in Saccharomyces cerevisiae. Homologous pairing (HP) is an essential reaction during homologous recombination, and is generally catalyzed by the RecA/Rad51 family of proteins in an ATP-dependent manner. Mhr1 catalyzes HP through a mechanism similar, at the DNA level, to that of the RecA/Rad51 proteins, but without utilizing ATP. However, it has no sequence homology with the RecA/Rad51 family proteins or with other ATP-independent HP proteins, and exhibits different requirements for DNA topology. We are interested in the structural features of the functional domains of Mhr1. In this study, we employed the native fluorescence of Mhr1's Trp residues to examine the energy transfer from the Trp residues to etheno-modified ssDNA bound to Mhr1. Our results showed that two of the seven Trp residues (Trp71 and Trp165) are spatially close to the bound DNA. A systematic analysis of mutant Mhr1 proteins revealed that Asp69 is involved in Mg2+ -dependent DNA binding, and that multiple Lys and Arg residues located around Trp71 and Trp165 are involved in the DNA-binding activity of Mhr1. In addition, in vivo complementation analyses showed that a region around Trp165 is important for the maintenance of mtDNA. On the basis of these results, we discuss the function of the region surrounding Trp165. [source]


Variation in essential oil composition of rose-scented geranium (Pelargonium sp.) distilled by different distillation techniques,

FLAVOUR AND FRAGRANCE JOURNAL, Issue 2 2005
Kiran G. D. Babu
Abstract The rose-scented geranium (Pelargonium sp.) cultivar ,Kelkar', grown in the agroclimatic conditions of the western Himalayas, was processed by various hydrodistillation methods, which revealed that water distillation of the herb gave a higher oil yield (0.16,0.22%) than the water,steam distillation (0.09,0.12%) and steam distillation methods (0.06,0.18%). The samples were analysed by GC and GC,MS to study and compare the essential oil compositions which revealed that the oil distilled by the water,steam distillation method contained a higher content of monoterpene hydrocarbons (1.7%), followed by steam distillation without cohobation and without recycling (1.5%). A higher content of sesquiterpene hydrocarbons (4.4%) was found in cumulative oil followed by ,direct oil' (4.2%) obtained by steam distillation with cohobation and without recycling of hydrosol, followed by the water,steam distillation method (3.4%). ,Decanted oil', recovered from redistilling the hydrosol obtained by steam distillation with cohobation and without recycling, contained maximum monoterpene cyclic ethers (1.1%) and carbonyl content (9.9%), closely followed by water,steam distillation method (1.1% and 7.2%, respectively). Steam distillation without cohobation and without recycling of hydrosol yielded essential oil with a higher percentage of esters (21.1%), followed by ,direct oil' (16.6%). Lower ester content (5.3%) was noticed in ,decanted oil', followed by oil distilled by steam distillation with cohobation and with recycling (11.8%) and oil distilled in a Clevenger apparatus by the water distillation method (12.2%), whereas maximum total alcohols were found in the ,decanted oil' (75.1%), followed by oil from the Clevenger apparatus (72.8%) and steam distillation with cohobation and with recycling (69.1%). A lower alcohol content was found in the ,direct oil' (55.2%) closely followed by ,cumulative oil' (55.8%). The variation in total alcohol and ester contents in geranium oil samples, distilled by using different processing techniques on pilot scale distillation, is mainly due to hydrolysis of some volatile constituents. This was further supported by acid values of the oils, along with other physicochemical properties, such as speci,c gravity, optical rotation, refractive index, solubility in alcohol, ester value with cold and hot methods, estimation of ester content as geranyl formate and geranyl tiglate, ester number after acetylation, and ester number after formylation with aceto-formic acid and formic acid. Methods have been standardized and proposed for distillations of speci,c quality, e.g. ester-rich and alcohol-rich geranium oils, to meet different requirements of the industry. Copyright © 2004 John Wiley & Sons, Ltd. [source]


Vitamin D receptor amounts across different segments of the gastrointestinal tract in Brown Swiss and Holstein Frisean cows of different age

JOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 3 2008
A. Liesegang
Summary During different stages of lactation, different requirements of calcium have to be met depending on the milk amount. Vitamin D receptors (VDR) regulate calcium homeostasis by increasing the entry of Ca into blood from bone stores and dietary sources. The purpose of this study was to investigate if age and breed of cows influence VDR amounts across different segments of the gastrointestinal tract. Thirty-six cows were used (18 brown swiss, 18 holstein friesan, both > 5.5 years or < 4.5 years). Tissue specimens of the intestines were collected from the cows. Formaldehyde-fixed and microwave-treated paraffin sections were used for VDR immunohistochemistry employing a biotinylated monoclonal rat antibody and streptavidin peroxidase technique. The results showed that nuclei and cytoplasm of enterocytes stained positively for VDRs. Strongest immunoreactions were observed in intermediate and basal glandular cells. No significant differences were observed between the different groups. Vitamin D receptors immunoreactivities were prominent in duodenal mucosa, lower in jejunum and in colon, decreased further in ileum and were lowest in caecum. Decreases in number of positively marked cells and staining intensities resulted in reduced immunoreactions. The results of this study indicate that VDR are highly expressed at the site of maximal intestinal calcium absorption. No significant influence of age and breed was observed. The animals used were not in a negative Ca balance. The cows were all in the stage of late or mid lactation. During these periods, the Ca requirements are low and the diets are high in Ca concentration; and the animals are adapted to these circumstances. Passive absorption in adult animals seems to dominate when Ca intake is adequate or high. The active absorption may play a considerably more significant role during the peripartal period, when Ca homeostatic mechanisms are challenged because of tremendous Ca demand at the initiation of lactation. [source]


Predicting spatio-temporal recolonization of large carnivore populations and livestock depredation risk: wolves in the Italian Alps

JOURNAL OF APPLIED ECOLOGY, Issue 4 2010
F. Marucco
Summary 1.,Wolves Canis lupus recently recolonized the Western Alps through dispersal from the Italian Apennines, representing one of several worldwide examples of large carnivores increasing in highly human-dominated landscapes. Understanding and predicting expansion of this population is important for conservation because of its direct impact on livestock and its high level of societal opposition. 2.,We built a predictive, spatially explicit, individual-based model to examine wolf population expansion in this fragmented landscape, and livestock depredation risk. We developed the model based on known demographic processes, social structure, behaviour and habitat selection of wolves collected during a 10-year intensive field study of this wolf population. 3.,During model validation, our model accurately described the recolonization process within the Italian Alps, correctly predicting wolf pack locations, pack numbers and wolf population size, between 1999 and 2008. 4.,We then projected packs and dispersers over the entire Italian Alps for 2013, 2018 and 2023. We predicted 25 packs (95% CI: 19,32) in 2013, 36 (23,47) in 2018 and 49 (29,68) in 2023. The South-Western Alps were the main source for wolves repopulating the Alps from 1999 to 2008. The source area for further successful dispersers will probably shift to the North-Western Alps after 2008, but the large lakes in the Central Alps will probably act as a spatial barrier slowing the wolf expansion. 5.,Using the pack presence forecasts, we estimated spatially explicit wolf depredation risk on livestock, allowing tailored local and regional management actions. 6.,Synthesis and applications. Our predictive model is novel because we follow the spatio-temporal dynamics of packs, not just population size, which have substantially different requirements and impacts on wolf,human conflicts than wandering dispersers. Our approach enables prioritization of management efforts, including minimizing livestock depredations, identifying important corridors and barriers, and locating future source populations for successful wolf recolonization of the Alps. [source]


Differential niche modification by males and females of a dioecious herb: extending the Jack Sprat effect

JOURNAL OF EVOLUTIONARY BIOLOGY, Issue 10 2010
J. SÁNCHEZ-VILAS
Abstract Males and females of dioecious plants often differ in morphological, physiological and life-history traits, probably as a result of their different requirements for reproduction. We found that the growth and reproductive effort of individuals of the dioecious herb Mercurialis annua depended on whether males or females had been growing in the soil previously. This suggests that males and females of M. annua differentially modify the soil in which they are growing. Our study indicates that sexual dimorphism in dioecious plants can give rise to increased environmental heterogeneity as a consequence of sex-specific niche modification. [source]


Poly(lactic acid) brushes grow longer at lower temperatures

JOURNAL OF POLYMER SCIENCE (IN TWO SECTIONS), Issue 15 2010
Lebo Xu
Abstract Poly(lactic acid) (PLA) brushes prepared by the ring opening of lactide were thicker when polymerized at a lower temperature (25 °C) than was typically used for polymerization in solution. The molecular weight of solution polymerized lactide was also higher when lactide was polymerized at 25 °C compared with polymerization at higher temperatures. However, the yield of PLA was low at this temperature. These results highlight the different requirements for solution polymerization and brush growth. In the former case, both percentage of conversion and molecular weight are important considerations. In the latter case, however, percentage of conversion is unimportant as a brush represents a very small amount of polymer. It was also shown during the course of these studies that the native hydroxy groups on silicon substrates and silanols in solution were equally good initiators when compared with hydroxy terminated self-assembled monolayers on gold and alcohols, respectively. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 3362,3367, 2010 [source]


Reliability aspects of SiC Schottky diodes

PHYSICA STATUS SOLIDI (A) APPLICATIONS AND MATERIALS SCIENCE, Issue 10 2009
Matthias Holz
Abstract In recent years, silicon carbide (SiC) high-voltage power devices have gained an ever-increasing market share. The fast development of new device concepts and technologies, e.g. for SiC Schottky diodes, has led to devices with superior switching behaviour, which renders SiC power devices especially favourable for high-frequency applications. As of today, SiC devices enter various fields like, e.g. server power supplies, solar inverters, and drives. These applications pose quite different requirements not only on the electrical properties, but also on the long-term reliability of the devices. In this paper, we describe in detail how Infineon's SiC Schottky diodes excel the reliability requirements. We point out how material properties, device design and packaging technology affect the overall device reliability and how they can be optimized. In addition, we describe measurement results after stress tests that go far beyond standard stress tests according to JEDEC. E.g., we show that SiC devices can safely be operated at high voltage slopes of 120 V/ns. In addition, we show that the use of high performance die attach further improves the device properties and reliability. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source]


Network board continuity and effectiveness of open innovation in Swedish strategic small-firm networks

R & D MANAGEMENT, Issue 1 2009
Joakim Wincent
Increasing adoption of open innovation as an alternative route to research and development necessitates the development of new ways to organize innovation, as well as reassessment of existing ways. Much like traditional corporations that subscribe to the closed innovation paradigm, novel organizational arrangements targeting open innovation, such as small-firm networks, employ boards to effectively manage joint research-and-development activities. These boards are similar yet different from traditional corporate boards; as such, they may have different requirements for proper functioning. We use 5-year longitudinal data on 53 Swedish strategic small-firm networks to investigate how the boards should be organized to help improve the innovative status of network participants. We expand the set of tools available for effective organization of the boards' operations and emphasize the effects of network board continuity (rates of renewal) on network members' innovative performance. We argue that the relationship is curvilinear (U-shaped) and demonstrate that it is more pronounced in larger networks. [source]


Delayed acquisition of somatic hypermutations in repopulated IGD+CD27+ memory B cell receptors after rituximab treatment

ARTHRITIS & RHEUMATISM, Issue 8 2009
Khalid Muhammad
Objective Transient B cell depletion by rituximab has been used with clinical efficacy in the treatment of patients with rheumatoid arthritis (RA). Previous studies of B cell repopulation have shown long-term numerical reduction in memory B cells. Non,class-switched IgD+CD27+ memory B cells, in particular, repopulate slowly. This study was undertaken to determine whether mutational acquisition in individual B cell receptors in repopulating class-switched and non,class-switched memory B cells is affected by rituximab. Methods Cells obtained from 16 RA patients, 4 healthy donors, and 3 patients who underwent allogeneic stem cell transplantation (ASCT) were analyzed using single B cell sorting followed by nested polymerase chain reaction and Ig VH3 sequencing. Results There was a delayed acquisition of mutations in Ig receptors of IgD+ memory B cells over a period of 6 years after a single course of rituximab. One year after rituximab treatment, 84% of single repopulating IgD+CD27+ B cells were unmutated, and no highly mutated Ig receptors were found (compared with 52% before therapy). Over time, increasing numbers of mutations were detected. Even 6 years after rituximab treatment, however, mutations in IgD+ memory B cells were still significantly reduced. In contrast, class-switched memory B cells repopulated with quantitatively normal mutations. In comparison, in patients undergoing ASCT, IgD+ memory cells repopulated earlier with higher mutations in Ig receptors. Conclusion Our data suggest that IgD+ memory B cells are particularly susceptible to the effects of rituximab, with delayed acquisition of mutations in their Ig receptors still evident 6 years after a single course of rituximab. Our findings indicate that these cells have different requirements for mutational acquisition compared with class-switched memory B cells. [source]


Structural insights into the assembly of the human and archaeal signal recognition particles

ACTA CRYSTALLOGRAPHICA SECTION D, Issue 3 2010
Klemens Wild
The signal recognition particle (SRP) is a conserved ribonucleoprotein (RNP) complex that co-translationally targets membrane and secretory proteins to membranes. The assembly of the particle depends on the proper folding of the SRP RNA, which in mammalia and archaea involves an induced-fit mechanism within helices 6 and 8 in the S domain of SRP. The two helices are juxtaposed and clamped together upon binding of the SRP19 protein to their apices. In the current assembly paradigm, archaeal SRP19 causes the asymmetric loop of helix 8 to bulge out and expose the binding platform for the key player SRP54. Based on a heterologous archaeal SRP19,human SRP RNA structure, mammalian SRP19 was thought not to be able to induce this change, thus explaining the different requirements of SRP19 for SRP54 recruitment. In contrast, the crystal structures of a crenarchaeal and the all-human SRP19,SRP RNA binary complexes presented here show that the asymmetric loop is bulged out in both binary complexes. Differences in SRP assembly between mammalia and archaea are therefore independent of SRP19 and are based on differences in SRP RNA itself. A new SRP-assembly scheme is presented. [source]