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Different Elements (different + element)
Selected AbstractsModels of policy-making and their relevance for drug researchDRUG AND ALCOHOL REVIEW, Issue 4 2010ALISON RITTER Abstract Introduction and Aims. Researchers are often frustrated by their inability to influence policy. We describe models of policy-making to provide new insights and a more realistic assessment of research impacts on policy. Design and Methods. We describe five prominent models of policy-making and illustrate them with examples from the alcohol and drugs field, before drawing lessons for researchers. Results. Policy-making is a complex and messy process, with different models describing different elements. We start with the incrementalist model, which highlights small amendments to policy, as occurs in school-based drug education. A technical/rational approach then outlines the key steps in a policy process from identification of problems and their causes, through to examination and choice of response options, and subsequent implementation and evaluation. There is a clear role for research, as we illustrate with the introduction of new medications, but this model largely ignores the dominant political aspects of policy-making. Such political aspects include the influence of interest groups, and we describe models about power and pressure groups, as well as advocacy coalitions, and the challenges they pose for researchers. These are illustrated with reference to the alcohol industry, and interest group conflicts in establishing a Medically Supervised Injecting Centre. Finally, we describe the multiple streams framework, which alerts researchers to ,windows of opportunity', and we show how these were effectively exploited in policy for cannabis law reform in Western Australia. Discussion and Conclusions. Understanding models of policy-making can help researchers maximise the uptake of their work and advance evidence-informed policy.[Ritter A, Bammer G. Models of policy-making and their relevance for drug research. Drug Alcohol Rev 2010] [source] A sediment budget for a cultivated floodplain in tropical North Queensland, AustraliaEARTH SURFACE PROCESSES AND LANDFORMS, Issue 10 2007Fleur Visser Abstract Sugarcane is grown on the floodplains of northern Queensland adjacent to the Great Barrier Reef lagoon. Sediment and nutrient loss from these sugarcane areas is considered a potential threat to coastal and marine ecosystems. To enable sugarcane cultivation, farmers have structured the landscape into different elements, comprising fields, water furrows, ,headlands' and drains. In order to apply appropriate management of the landscape and reduce export of sediment, it is important to identify which of these elements act as sediment sources or sinks. In this study erosion and deposition rates were measured for the different landscape elements in a subcatchment of the Herbert River and used to create a sediment budget. Despite large uncertainties, the budget shows that the floodplain area is a net source of sediment. Estimated sediment export varies between 2 and 5 t ha,1 y,1. The relative importance of the landscape elements as sediment sources could also be determined. Plant cane is identified as the most important sediment source. Water furrows generate most sediment, but are a less important source of exported sediment due to their low connectivity. Headlands and minor drains act as sediment traps. Copyright © 2007 John Wiley & Sons, Ltd. [source] A Quantified Ethogram for Oviposition in Triturus Newts: Description and Comparison of T. helveticus and T. vulgarisETHOLOGY, Issue 4 2005Karen M. Norris Female newts of the genus Triturus deposit and wrap their eggs individually in the submerged leaves of aquatic macrophytes. Although this behaviour has previously been described, the different elements of the oviposition process have not been fully characterized nor any attempt made to quantify the behavioural elements. The study examined the oviposition behaviour of the two similarly sized species, Triturus helveticus and T. vulgaris on a standardized substrate macrophyte, Rorippa nasturtium,aquaticum. Continuous focal sampling was used to develop a baseline of discrete behavioural elements enabling quantification and comparison of oviposition behaviour between the two species. The results showed that the same pattern of elements was followed for each egg laid and the same key elements of the process were present in each newt species. Although these are broadly similar in size, there were striking differences in certain aspects of the oviposition sequence between the two species. Key findings were that leaf sniffing and leaf flexing and a measure of the duration of ovipositing were all significantly greater in females of T. helveticus and females of T. vulgaris laid significantly more eggs than those of T. helveticus in a standard observation period. The work presented here defines a baseline ethogram and shows how it can be used to reveal quantifiable differences in closely related species. This demonstrates its value in furthering our understanding of oviposition , a key aspect of female behaviour currently understudied in Triturus behavioural ecology, despite its intrinsic interest and value in understanding recruitment and maintenance of populations. [source] Study of the regulation of the endocannabinoid system in a virus model of multiple sclerosis reveals a therapeutic effect of palmitoylethanolamideEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 4 2008Frida Loría Abstract Cannabinoids have recently been approved as a treatment for pain in multiple sclerosis (MS). Increasing evidence from animal studies suggests that this class of compounds could also prove efficient to fight neurodegeneration, demyelination, inflammation and autoimmune processes occurring in this pathology. However, the use of cannabinoids is limited by their psychoactive effects. In this context, potentiation of the endogenous cannabinoid signalling could represent a substitute to the use of exogenously administrated cannabinoid ligands. Here, we studied the expression of different elements of the endocannabinoid system in a chronic model of MS in mice. We first studied the expression of the two cannabinoid receptors, CB1 and CB2, as well as the putative intracellular cannabinoid receptor peroxisome proliferator-activated receptor-,. We observed an upregulation of CB2, correlated to the production of proinflammatory cytokines, at 60 days after the onset of the MS model. At this time, the levels of the endocannabinoid, 2-arachidonoylglycerol, and of the anti-inflammatory anandamide congener, palmithoylethanolamide, were enhanced, without changes in the levels of anandamide. These changes were not due to differences in the expression of the degradation enzymes, fatty acid amide hydrolase and monoacylglycerol lipase, or of biosynthetic enzymes, diacylglycerol lipase-, and N -acylphosphatidylethanolamine phospholipase-D at this time (60 days). Finally, the exogenous administration of palmitoylethanolamide resulted in a reduction of motor disability in the animals subjected to this model of MS, accompanied by an anti-inflammatory effect. This study overall highlights the potential therapeutic effects of endocannabinoids in MS. [source] Democratic Accountability and National Parliaments: Redefining the Impact of Parliamentary Scrutiny in EU AffairsEUROPEAN LAW JOURNAL, Issue 4 2007Katrin Auel Such an evaluation, however, is flawed: Formal mandating rights are usually incompatible with the overall logic of parliamentary systems, which explains why most national parliaments make very little use of them. Even more importantly, it unduly reduces parliamentary functions to the legislative or policy-making function. Drawing on agency theory, it will instead be argued that the functions of public deliberation and of holding the government publicly to account are at least as important and therefore need to be included in a redefined concept of parliamentary strength. In particular, the article proposes a distinction between two different elements of accountability,monitoring and political scrutiny,which recognises parliamentary majority and opposition as two distinct agents of the electorate. [source] A comparative analysis of the diving behaviour of birds and mammalsFUNCTIONAL ECOLOGY, Issue 5 2006L. G. HALSEY Summary 1We use a large interspecific data set on diving variables for birds and mammals, and statistical techniques to control for the effects of phylogenetic non-independence, to assess evolutionary associations among different elements of diving behaviour across a broad and diverse range of diving species. Our aim is to assess whether the diving ability of homeothermic vertebrates is influenced by factors other than the physiology of the species. 2Body mass is related to dive duration even when dive depth is controlled for and thus for a given dive depth, larger species dive for longer. This implies that larger species have a greater capacity for diving than is expressed in their dive depth. Larger animals that dive shallowly, probably for ecological reasons such as water depth, make use of the physiological advantage that their size confers by diving for longer. 3Dive duration correlates with dive depth more strongly than with body mass. This confirms that some animals are poor divers for their body mass, either because of a lower physiological capacity or because their behaviour limits their diving. 4Surface duration relates not only to dive duration but also to dive depth, as well as to both independently. This indicates a relationship between dive depth and surface duration controlling for dive duration, which suggests that deeper dives are energetically more expensive than shallow dives of the same duration. 5Taxonomic class does not improve any of the dive variable models in the present study. There is thus an unsuspected consistency in the broad responses of different groups to the effects on diving of the environment, which are therefore general features of diving evolution. [source] Origin and geochemistry of Miocene marine evaporites associated with red beds: Great Kavir Basin, Central IranGEOLOGICAL JOURNAL, Issue 1 2007Hossain Rahimpour-Bonab Abstract During the Cenozoic numerous shallow epicontinental evaporite basins formed due to tectonic movements in the Northern Province of the Central Iran Tectonic Zone (the Great Kavir Basin). During the Miocene, due to sea-level fluctuations, thick sequences of evaporites and carbonates accumulated in these basins that subsequently were overlain by continental red beds. Development of halite evaporites with substantial thickness in this area implies inflow of seawater along the narrow continental rift axis. The early ocean basin development was initiated in Early Eocene time and continued up to the Middle Miocene in the isolated failed rift arms. Competition between marine and non-marine environments, at the edge of the encroaching sea, produced several sequences of both abrupt and gradual transition from continental wadi sediments to marginal marine evaporites in the studied area. These evaporites show well-preserved textures indicative of relatively shallow-brine pools. The high Br content of these evaporites indicates marine-derived parent brines that were under the sporadic influence of freshening by meteoric water or replenishing seawater. However, the association of hopper and cornet textures denotes stratified brine that filled a relatively large pool and prevented rapid variations in the Br profile. Unstable basin conditions that triggered modification of parent brine chemistry prevailed in this basin and caused variable distribution patterns for different elements in the chloride units. The presence of sylvite and the absence of Mg-sulphate/chlorides in the paragenetic sequence indicate SO4,depleted parent brine in the studied sequence. Petrographic examinations along with geochemical analyses on these potash-bearing halites reveal parental brines which were a mixture of seawater and CaCl2 -rich brines. The source of CaCl2 -rich brines is ascribed to the presence of local rift systems in the Great Kavir Basin up to the end of the Early Miocene. Copyright © 2007 John Wiley & Sons, Ltd. [source] Toward large scale F.E. computation of hot forging process using iterative solvers, parallel computation and multigrid algorithmsINTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN ENGINEERING, Issue 5-6 2001K. Mocellin Abstract The industrial simulation code Forge3® is devoted to three-dimensional metal forming applications. This finite element software is based on an implicit approach. It is able to carry out the large deformations of viscoplastic incompressible materials with unilateral contact conditions. The finite element discretization is based on a stable mixed velocity,pressure formulation and tetrahedral unstructured meshes. Central to the Newton iterations dealing with the non-linearities, a preconditioned conjugate residual method (PCR) is used. The parallel version of the code uses an SPMD programming model and several results on complex applications have been published. In order to reduce the CPU time computation, a new solver has been developed which is based on multigrid theory. A detailed presentation of the different elements of the method is given: the geometrical approach based on embedded meshes, the direct resolution of the velocity,pressure system, the use of PCR method as an original smoother and for solving the coarse problem, the full multigrid method and the required preconditioning by an incomplete Cholesky factorization for problems with complex contact conditions. By considering different forging cases, the theoretical properties of the multigrid method are numerically verified, optimizations of the solver are presented and finally, the results obtained on several industrial problems are given, showing the efficiency of the new solver that provides speed-up larger than 5. Copyright © 2001 John Wiley & Sons, Ltd. [source] A comparative study of GLS finite elements with velocity and pressure equally interpolated for solving incompressible viscous flowsINTERNATIONAL JOURNAL FOR NUMERICAL METHODS IN FLUIDS, Issue 5 2009Yongtao Wei Abstract A comparative study of the bi-linear and bi-quadratic quadrilateral elements and the quadratic triangular element for solving incompressible viscous flows is presented. These elements make use of the stabilized finite element formulation of the Galerkin/least-squares method to simulate the flows, with the pressure and velocity fields interpolated with equal orders. The tangent matrices are explicitly derived and the Newton,Raphson algorithm is employed to solve the resulting nonlinear equations. The numerical solutions of the classical lid-driven cavity flow problem are obtained for Reynolds numbers between 1000 and 20 000 and the accuracy and converging rate of the different elements are compared. The influence on the numerical solution of the least square of incompressible condition is also studied. The numerical example shows that the quadratic triangular element exhibits a better compromise between accuracy and converging rate than the other two elements. Copyright © 2008 John Wiley & Sons, Ltd. [source] Focused-Ion-Beam-Based Selective Closing and Opening of Anodic Alumina Nanochannels for the Growth of Nanowire Arrays Comprising Multiple Elements,ADVANCED MATERIALS, Issue 13 2008Nai-Wei Liu A lithographic process based on focused ion beam bombardment is developed for selectively closing and opening nanochannels on a porous anodic alumina film. This resist-free process is based on the use of focused ion beams with different energies that strike a balance between material sputtering and material relocation. This process is used to selectively grow nanowire patterns of different elements. [source] Puzzling practice: A strategy for working with clinical practice issuesINTERNATIONAL JOURNAL OF NURSING PRACTICE, Issue 2 2008Kenneth Walsh RPN RN BNurs PhD In this paper we aim to share the evolution of innovative ways to explore, ,unpack' and reframe clinical issues that exist in everyday practice. The elements of these processes, which we call ,puzzling practice', and the techniques associated with them, were delineated over a two year period by the four authors using action theory based processes. The authors have evolved several different frameworks for ,puzzling practice' which we draw on and use in our practice development work and in our research practice. This paper pays attention to a particular form of puzzling practice that we have found to be useful in assisting individual clinicians and teams to explore and find workable solutions to practice issues. The paper uses a semi-fictitious example of ,Puzzling Practice' gleaned from our experience as practice development facilitators. In this example ,puzzling practice' uses seven different elements; naming the issue; puzzling the issue; testing the puzzle exploring the heart of out practice; formulating the puzzle question; visualizing the future; and generating new strategies for action. Each of the elements is illustrated by the story and the key foundations and ideas behind each element is explored. [source] Implementing a pre-operative checklist to increase patient safety: a 1-year follow-up of personnel attitudesACTA ANAESTHESIOLOGICA SCANDINAVICA, Issue 2 2010L. NILSSON Background: The operating room is a complex work environment with a high potential for adverse events. Protocols for perioperative verification processes have increasingly been recommended by professional organizations during the last few years. We assessed personnel attitudes to a pre-operative checklist (,time out') immediately before start of the operative procedure. Methods: ,Time out' was implemented in December 2007 as an additional safety barrier in two Swedish hospitals. One year later, in order to assess how the checklist was perceived, a questionnaire was sent by e-mail to 704 persons in the operating departments, including surgeons, anesthesiologists, operation and anesthetic nurses and nurse assistants. In order to identify differences in response between professions, each alternative in the questionnaire was assigned a numerical value. Results: The questionnaire was answered by 331 (47%) persons and 93% responded that ,time out' contributes to increased patient safety. Eighty-six percent thought that ,time out' gave an opportunity to identify and solve problems. Confirmation of patient identity, correct procedure, correct side and checking of allergies or contagious diseases were considered ,very important' by 78,84% of the responders. Attitudes to checking of patient positioning, allergies and review of potential critical moments were positive but differed significantly between the professions. Attitudes to a similar checklist at the end of surgery were positive and 72,99% agreed to the different elements. Conclusion: Staff attitudes toward a surgical checklist were mostly positive 1 year after their introduction in two large hospitals in central Sweden. [source] An update on the mechanisms of the psychostimulant effects of caffeineJOURNAL OF NEUROCHEMISTRY, Issue 4 2008Sergi Ferré Abstract There has been a long debate about the predominant involvement of the different adenosine receptor subtypes and the preferential role of pre- versus post-synaptic mechanisms in the psychostimulant effects of the adenosine receptor antagonist caffeine. Both striatal A1 and A2A receptors are involved in the motor-activating and probably reinforcing effects of caffeine, although they play a different role under conditions of acute or chronic caffeine administration. The present review emphasizes the key integrative role of adenosine and adenosine receptor heteromers in the computation of information at the level of the striatal spine module (SSM). This local module is mostly represented by the dendritic spine of the medium spiny neuron with its glutamatergic and dopaminergic synapses and astroglial processes that wrap the glutamatergic synapse. In the SSM, adenosine acts both pre- and post-synaptically through multiple mechanisms, which depend on heteromerization of A1 and A2A receptors among themselves and with D1 and D2 receptors, respectively. A critical aspect of the mechanisms of the psychostimulant effects of caffeine is its ability to release the pre- and post-synaptic brakes that adenosine imposes on dopaminergic neurotransmission by acting on different adenosine receptor heteromers localized in different elements of the SSM. [source] ASSESSING CONSUMER EXPECTATIONS FOR FOOD BARS BY CONJOINT ANALYSISJOURNAL OF SENSORY STUDIES, Issue 6 2009K. MAHANNA ABSTRACT Food bars, which include meal replacement, protein, granola and cereal bars, have rapidly increased in sales with current estimation of above $3.2 billion. Despite such rapid growth in the market, research on consumer expectations for these products is still lacking. Conjoint analysis was used to probe the effect of different elements of food bar products on purchase intent, including types of bar, ingredients, macro-nutrients, health claims, calories and sensory characteristics. Overall, consumers wanted a food bar to be less than 150 kcal, made with whole grains and higher in protein. Consumers divided into four mind-set segments were characterized as "Label Readers" (n = 213), "Calorie Health Nuts" (n = 90), "Flavor Energy Seekers" (n = 79) and "Chocolate Lovers" (n = 44). Sensory characteristics and types of bar categories were not important to consumers, although calorie elements played the largest role in motivating purchase intent for food bars. PRACTICAL APPLICATIONS The results found from this research help to guide new product development in the food bar market by identifying the desires of specific consumer segments. Consumers were interested in food bar products with lower calorie content, which supports the idea that food bars are more typically consumed as a snack than a meal replacement. Half of the sample consumers tested belonged to the "Label Readers" segment. These consumers are interested in a healthful food bar product high in fiber. Development of a bar that is considered healthful would appeal not only to the "Label Readers" segment, but also to the "Calorie Health Nuts" segment. When developing a food bar product, the labeled bar type or sensory claims are not important to consumers. Consumers are more interested in the ingredients and caloric content of the food bar. [source] UK consensus statement on the content of communication curricula in undergraduate medical educationMEDICAL EDUCATION, Issue 11 2008Martin Von Fragstein Context, The teaching and assessment of clinical communication have become central components of undergraduate medical education in the UK. This paper recommends the key content for an undergraduate communication curriculum. Designed by UK educationalists with UK schools in mind, the recommendations are equally applicable to communication curricula throughout the world. Objectives, This paper is intended to assist curriculum planners in the design, implementation and review of medical communication curricula. The document will also be useful in the education of other health care professionals. Designed for undergraduate education, the consensus statement also provides a baseline for further professional development. Methods, The consensus statement, based on strong theoretical and research evidence, was developed by an iterative process of discussion between communication skills leads from all 33 UK medical schools conducted under the auspices of the UK Council of Clinical Communication Skills Teaching in Undergraduate Medical Education. Discussion, How this framework is used will inevitably be at the discretion of each medical school and its implementation will be determined by different course designs. Although we believe students should be exposed to all the areas described, it would be impractical to set inflexible competency levels as these may be attained at different stages which are highly school-dependent. However, the framework will enable all schools to consider where different elements are addressed, where gaps exist and how to generate novel combinations of domains within the communication curriculum. It is hoped that this consensus statement will support the development and integration of teaching, learning and assessment of clinical communication. [source] Mapping the intake of different elements in vegetal tissues by dual-energy X-ray imaging at Da,ne synchrotron light sourceMICROSCOPY RESEARCH AND TECHNIQUE, Issue 3 2008L. Reale Abstract This article reports on the first utilization of the soft X-ray beamline at the Da,ne synchrotron light source for mapping the intake of different elements in plant tissues. As a test, the method of dual-energy X-ray microradiography was applied to the investigation of the natural sulfur content in dried leaf and root samples. Our ultimate goal was to monitor the pollutant lead and its intake, which was added in controlled doses to the hydroponic medium of laboratory-controlled samples of vegetal species. The results obtained by the nondestructive X-ray radiographic analysis are compared to the values of concentrations determined by a standard chemical analysis utilizing atomic absorption spectroscopy. From this comparison the validity of the X-ray detection of heavy metals in biological samples has been confirmed. The superposition of the dual energy results on the simple planar radiography shows the representation of the pollutant intake directly on the sample structures. It should be pointed out that this method, developed here for plant root and leaves could be applied to any biological sample of interest, but the preparation and observation conditions necessitate different strategies according to the type of sample under analysis. Microsc. Res. Tech., 2008. © 2007 Wiley-Liss, Inc. [source] Applying Conceptual Grammar to Advanced-Level Language Teaching: The Case of Two Completive Constructions in KoreanMODERN LANGUAGE JOURNAL, Issue 2 2006SUSAN STRAUSS This article introduces conceptual grammar as an approach to the analysis and teaching of grammar in foreign and second language contexts through a combination of paradigms: corpus, discourse analysis, and cognitive linguistics. Although the approach is applicable to virtually any language and any construction within that language at various levels of study, we provide a detailed demonstration using Korean as a model. In particular, we focus on constructions expressing the completive aspect. The Korean system of marking aspect can be quite complex; what renders the Korean completive even more perplexing is the fact that it is expressed through two seemingly similar auxiliary forms, each of which signals different elements in the speaker's or writer's stance vis-à-vis the described event. By combining the paradigms of corpus, discourse analysis, and cognitive linguistics, the article demonstrates how a conceptual grammatical approach can render salient the particular discursive and conceptual patterns underlying the target forms. It is designed as a pedagogical tool to guide users to discern both inductively and deductively how native speakers conceptualize these differences and express them morphosyntactically,a perspective that is absent from most existing reference grammars and textbooks. In this article, we present samples of pedagogical materials developed using this model in addition to results of an experiment in which a version of those materials was administered to teachers and students of advanced Korean. [source] Why does elevated CO2 affect time of flowering?NEW PHYTOLOGIST, Issue 2 2009An exploratory study using the photoperiodic flowering mutants of Arabidopsis thaliana Summary ,,Evidence is accumulating that the effect of CO2 on time of flowering involves interactions with photoperiod, but the basis for this interaction is unclear. Here, which components of the photoperiod flowering pathway account for this interaction in Arabidopsis thaliana were examined. ,,Ten mutants deficient in particular loci in the photoperiod pathway, as well as the wild type, were grown under short and long days at either ambient or elevated CO2. Leaf number at flowering and the number of days required for induction of flowering were determined. ,,Elevated CO2 interacted with both the photoreceptors and the subsequent transduction reactions in the photoperiod pathway. The direction and magnitude of the effects varied with photoperiod. Elevated CO2 also affected flowering by increasing rate of leaf production. ,,The net effect of elevated CO2 on time of flowering varies because CO2 has a complex array of effects on different elements of the developmental pathway leading to flower induction that may either hasten or delay flowering depending upon the influence of other environmental factors such as photoperiod. [source] Non-adiabatic small polaron hopping conduction in sodium borate tungstate glassesPHYSICA STATUS SOLIDI (A) APPLICATIONS AND MATERIALS SCIENCE, Issue 2 2003A. Al-Shahrani Abstract The dc electrical conductivity of (100,x)Na2B4O7,xWO3 (x = 5, 15, 20 and 30 mol%) glasses is reported in the temperature range 323,473 K. The density and molar volume for these glasses are consistent with the ionic size, atomic weight and amount of different elements in the glasses. At high temperatures the Mott model of phonon-assisted small polaron hopping between nearest neighbours is consistent with conductivity data, while at intermediate temperatures the Greaves variable-range hopping model is found to be appropriate. The estimated model parameters such as number of ions per unit volume, hopping distance, polaron radius and activation energy are found to be consistent with the formation of localized states in these glasses. The electrical conduction of these glasses is confirmed to be that of non-adiabatic small polaron hopping. (© 2003 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] The way composition affects martensitic transformation temperatures of Ni,Mn,Ga Heusler alloysPHYSICA STATUS SOLIDI (B) BASIC SOLID STATE PHYSICS, Issue 3 2007X. Q. Chen Abstract A systematic substitution of Ge, Si, C and Co for Ga in the non-stoichiometric Ni,Mn,Ga alloys was performed. The relationship between the compositions of different elements including Ni, Mn, Ga, Ge, Si, C, Co, In and martensitic transformation temperature (Ms) was studied in detail for the present alloys together with data collected from a variety of sources. It is found that Ms is a sensitive parameter to the composition. The size factor and electron concentration are usually thought to be the way the composition influences Ms in the Ni,Mn,Ga alloys. Here, analyzing by linear regression, the electron density maybe a most appropriate parameter to describe the way the composition influences Ms when compared with size factor and electron concentration. (© 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] Transcriptionally active transposable elements in recent hybrid sugarcaneTHE PLANT JOURNAL, Issue 5 2005Paula G. de Araujo Summary Transposable elements (TEs) are considered to be important components of the maintenance and diversification of genomes. The recent increase in genome sequence data has created an opportunity to evaluate the impact of these active mobile elements on the evolution of plant genomes. Analysis of the sugarcane transcriptome identified 267 clones with significant similarity to previously described plant TEs. After full cDNA sequencing, 68 sugarcane TE clones were assigned to 11 families according to their best sequence alignment against a fully characterized element. Expression was further investigated through a combined study utilizing electronic Northerns, macroarray, transient and stable sugarcane transformation. Newly synthesized cDNA probes from flower, leaf roll, apical meristem and callus tissues confirm previous results. Callus was identified as the tissue with the highest number of TEs being expressed, revealing that tissue culture drastically induced the expression of different elements. No tissue-specific family was identified. Different representatives within a TE family displayed differential expression patterns, showing that each family presented expression in almost every tissue. Transformation experiments demonstrated that most Hopscotch clone-derived U3 regions are, indeed, active promoters, although under a strong transcriptional regulation. This is a large-scale study about the expression pattern of TEs and indicates that mobile genetic elements are transcriptionally active in the highly polyploid and complex sugarcane genome. [source] Multiple GUS expression patterns of a single Arabidopsis geneANNALS OF APPLIED BIOLOGY, Issue 1 2009Ekrem Dündar Abstract Ten independent transposant lines with gene or enhancer traps (ET) inserted into the same gene (At2g01170) were identified in Arabidopsis thaliana. Transposon insertions were confirmed for each line. Only three of five ET lines and only one of the five gene trap (GT) lines displayed uidA (GUS) staining. The GUS (,-glucuronidase) expression patterns of the ET lines were different in all three lines. In the GT line, the GUS expression was restricted to the vascular tissue under all conditions examined. The variation in ET GUS expression suggests that each ET was controlled by different enhancer elements or the different elements of the trapped locus may give rise to different GUS expression patterns. Of five GT lines, three have the GUS gene in the same orientation as the At2g01170 open reading frame, yet only one yielded GUS staining. Regardless of the insertion construct, only those transposants with an insertion at the 3, end of the gene yielded GUS staining. Some transposants displayed a longer root phenotype in the presence of kanamycin that was also observed in 3, insertion sites in At2g01170. Taken together, these data show that insertions in the 5, end of the gene disrupted expression and emphasise the complexity encountered with ET and GT constructs to characterise the expression patterns of genes of interest based solely on GUS expression patterns. [source] Dissecting membrane protein architecture: An annotation of structural complexityBIOPOLYMERS, Issue 10 2009Jaime Arce Abstract ,-Helical membrane proteins exist in an anisotropic environment which strongly influences their folding, stability, and architecture, which is far more complex than a simple bundle of transmembrane helices, notably due to helix deformations, prosthetic groups and extramembrane structures. However, the role and the distribution of such heterogeneity in the supra molecular organization of membrane proteins remains poorly investigated. Using a nonredundant subset of ,-helical membrane proteins, we have annotated and analyze the statistics of several types of new elements such as incomplete helices, intramembrane loops, helical extensions of helical transmembrane domains, extracellular loops, and helices lying parallel to the membrane surface. The relevance of the annotation scheme was studied using residue composition, statistics, physical chemistry, and symmetry of their distribution in relation to the immediate membrane environment. Calculation of hydrophobicity using different scales show that different structural elements appear to have affinities coherent with their position in the membrane. Examination of the annotation scheme suggests that there is considerable information content in the amino acid compositions of the different elements suggesting that it might be useful for structural prediction. More importantly, the proposed annotation will help to decipher the complex hierarchy of interactions involved in membrane protein architecture. © 2009 Wiley Periodicals, Inc. Biopolymers 91: 815,829, 2009. This article was originally published online as an accepted preprint. The "Published Online" date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com [source] East Asian monsoon instability at the stage 5a/4 transitionBOREAS, Issue 2 2002SHANGFA XIONG The physics involved in the abrupt climate changes of the late Quaternary have eluded paleoclimatologists for many years. More paleoclimatic records characteristic of different elements of the global climate system are needed for better understanding of the cause-feedback relationships in the system. The East Asian monsoon is an important part of the global climate system and the mechanical links between the East Asian monsoon and other climatic elements around the world may hold a key to our knowledge of abrupt climate changes in East Asia and probably over a larger part of the globe. Previous studies have detected millennial-scale winter monsoon oscillations during the last glaciation and probably also during the last interglaciation in loess sequences across China. However, less attention has been paid to the abrupt summer monsoon changes and the stage 5a/4 transition, an important period for the evolution of the East Asian monsoon when the global climate shifted towards the last glaciation. Here we report on two loess sections from eastern China which were dated using a thermoluminescence (TL) technique. The pedogenic and other sediment parameters suggest that the summer monsoon experienced a two-step abrupt retreat at the stage 5a/4 transition. The variations in the proxies for the winter monsoon are synchronized with the summer monsoon proxies during this brief interval, implying a direct and immediate link between high latitude and low latitude mechanisms. These changes may be correlated with similar climatic oscillations observed in the North Atlantic, Europe and Antarctica, raising the possibility that the forcing factors that induced these changes are global in extent. [source] | ||||||||||||||||||||||||||||||||||||||||