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Different Arrangements (different + arrangement)
Selected AbstractsDIFFERENT ARRANGEMENT OF ,-(,-GLUTAMYL)LYSINE CROSS-LINKING IN ALASKA POLLOCK (THERAGRA CHALCOGRAMMA) SURIMI PROTEINS BY STREPTOVERTICILLIUM AND ENDOGENOUS TRANSGLUTAMINASES DURING SUWARI PROCESSJOURNAL OF FOOD BIOCHEMISTRY, Issue 5 2001KENJI SATO ABSTRACT The objective of the present study is to compare the protein cross-linking reaction in Alaska pollock surimi that is catalyzed by a commercially available microbial transglutaminase and by endogenous Alaska pollock transglutaminase. The endogenous transglutaminase was inhibited by EGTA and activated by CaCl2 The microbial transglutaminase was added to the salted surimi with and without EGTA and CaCl2. These surimi pastes were incubated at 25C up to 24 h followed by cooking at 90C. The resultant gels were fractionated into soluble and insoluble (aggregate) fractions by SDS-urea extraction. Compositional analysis revealed that the aggregate consisted predominantly of cross-linked myosin heavy chain. The distribution of ,-(,-glutamyl)lysine isopeptide in the soluble and aggregate fractions andpeptide mapping analyses of the aggregate fraction demonstrate that the formation of isopeptide cross-links in Alaska pollock surimi proteins during suwari process differs when catalyzed by the microbial transglutaminase and endogenous transglutaminase. [source] Reduced ethanol response in the alcohol-preferring RHA rats and neuropeptide mRNAs in relevant structuresEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 2 2006Marc Guitart-Masip Abstract Roman rat strains, genetically selected for high (RHA) or low (RLA) active avoidance acquisition in the two-way shuttle box, differ in dopaminergic activity. These two strains appear to be a valid laboratory model of divergent sensation/novelty and substance-seeking profiles. RHA rats show higher ethanol intake and preference than do RLA rats, and it was suggested that RHA rats are more tolerant than RLA to the effects of alcohol. In the hole-board test, we found that the non-alcohol-preferring RLA rats showed enhanced responsiveness to the stimulatory effects of intraperitoneal administration of 0.25 g/kg ethanol when compared with RHA rats. In situ hybridization analysis showed higher levels of preprodynorphin in the accumbens shell and higher levels of preproenkephalin in the cingulate cortex in RHA rats. RLA rats showed higher levels of enkephalin gene transcripts in restricted areas of the dorsal striatum. Finally, differences in cholecystokinin gene transcript, suggestive of a different arrangement of certain interneurons, were found in different cortical areas. The differences in peptide gene expression found between the two strains might reflect the differences in alcohol preference and sensitivity. RHA rats may have more predictive value than other rodent alcoholism models, as high initial tolerance to ethanol is a risk factor for alcoholism in humans. [source] Structure elucidation and 3D solution conformation of the antibiotic enduracidin determined by NMR spectroscopy and molecular dynamicsMAGNETIC RESONANCE IN CHEMISTRY, Issue 8 2005F. Castiglione Abstract Enduracidin and ramoplanin belong to the large family of cyclodepsipeptide antibiotics, highly effective against Gram-positive bacteria. The primary and 3D solution structure of ramoplanin is already well known, and the primary structure of enduracidin has been determined by a combination of chemical and NMR spectroscopic methods. Both antibiotics share a similar peptide core of 17 amino acids and differ mainly in the length of the acyl chain and the presence of two D -mannose moieties in ramoplanin. Based on the high sequence homology with ramoplanin, the structure in solution of enduracidin is modeled as a cyclic peptide. The tertiary structure thus obtained was refined through molecular dynamics (MD) simulation, in which the interatomic NOE-derived distance restraints were imposed. MD simulations yielded a family of representative 3D structures (RMSD = 0.89), which highlighted a backbone geometry similar to that of ramoplanin in its ,-hairpin arrangement. In contrast, enduracidin displays a different arrangement of the side-chain and of the residues forming the hydrophobic core. Copyright © 2005 John Wiley & Sons, Ltd. [source] New anomalocaridid appendages from the Burgess Shale, CanadaPALAEONTOLOGY, Issue 4 2010ALLISON C. DALEY Abstract:, The complex history of description of the anomalocaridids has partly been caused by the fragmentary nature of these fossils. Frontal appendages and mouth parts are more readily preserved than whole-body assemblages, so the earliest work on these animals examined these structures in isolation. After several decades of research, these disarticulated elements were assembled together to reconstruct the anomalocaridid body plan, and a total of three Burgess Shale genera, Anomalocaris, Laggania and Hurdia, were described in full. Here we present new frontal appendage material of additional anomalocaridid taxa from the ,Middle' Cambrian (Series 3) Burgess Shale Formation in Canada, showing that the diversity of anomalocaridids in this locality is even higher than previously thought. Material includes Amplectobelua stephenensis sp. nov., the first known occurrence of this genus outside of China; Caryosyntrips serratus gen. et sp. nov., which is similar to the Anomalocaris appendage but has a straighter outline and a different arrangement of spines; and an appendage that may be either the Laggania appendage or a third morph of the Hurdia appendage. The new anomalocaridid material is contemporaneous with the previously described taxa Anomalocaris, Laggania, and Hurdia, and the differences in morphology between the frontal appendages may reflect different feeding strategies. The stratigraphically lowest locality, S7 on Mount Stephen, yields material from all anomalocaridid taxa, but the assemblages in the younger quarries on Fossil Ridge are dominated by Anomalocaris and Hurdia only. [source] The effect of absorption layer of different quantum well arrangement on optoelectronic characteristics of nitride-based photovoltaic cells grown by MOCVDPHYSICA STATUS SOLIDI (C) - CURRENT TOPICS IN SOLID STATE PHYSICS, Issue S2 2009Y. K. Fu Abstract In this study, III-nitride solar cells with multi-quantum well (MQW) absorption layer were grown on sapphire substrates by metal organic chemical vapor deposition (MOCVD). The effect of different quantum well (QW) arrangement on optoelectronic characteristics of III-nitrides photovoltaic cells was investigated. It was found that the upper quantum well (QW) layer will dominate electroluminescence (EL) emission mechanism and the electrical characteristics of solar cell. The advantage of modulating the short-circuit current density (JSC) and open-circuit voltage (VOC) can be obtained by different arrangement of blue and green QW in MQW absorption layer. The optimum electrical characteristics of solar cell with a JSC of 0.30 mA/cm2, a VOC up to 1.51 V, fill factor (FF) as high as 0.601, and a series resistance (RS) of 9 , can be obtained by using MQW absorption layer. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] The effect of a proline residue on the rate of growth and the space group of ,-spectrin SH3-domain crystalsACTA CRYSTALLOGRAPHICA SECTION D, Issue 12 2009Ana Cámara-Artigas ,-Spectrin SH3-domain (Spc-SH3) crystallization is characterized by very fast growth of the crystals in the presence of ammonium sulfate as a precipitant agent. The origin of this behaviour can be attributed to the presence of a proline residue that participates in a crystal contact mimicking the binding of proline-rich sequences to SH3 domains. This residue, Pro20, is located in the RT loop and is the main contact in one of the interfaces present in the orthorhombic Spc-SH3 crystal structures. In order to understand the molecular interactions that are responsible for the very fast crystal growth of the wild-type (WT) Spc-SH3 crystals, the crystal structure of a triple mutant in which the residues Ser19-Pro20-Arg21 in the RT loop have been replaced by Gly19-Asp20-Ser21 (GDS Spc-SH3 mutant) has been solved. The removal of the critical proline residue results in slower nucleation of the Spc-SH3 crystals and a different arrangement of the protein molecules in the unit cell, leading to a crystal that belongs to the tetragonal space group P41212, with unit-cell parameters a = b = 42.231, c = 93.655,Å, and that diffracts to 1.45,Å resolution. For both WT Spc-SH3 and the GDS mutant, light-scattering experiments showed that a dimer was formed in solution within a few minutes of the addition of 2,M ammonium sulfate at pH 6.5 and allowed the proposal of a mechanism for the nucleation and crystal growth of Spc-SH3 in which the Pro20 residue plays a key role in the rate of crystal growth. [source] Boundary element simulation of DC stray currents in oil industry due to cathodic protection interferenceEUROPEAN TRANSACTIONS ON ELECTRICAL POWER, Issue 5 2007I. A. Metwally Abstract This paper presents a 3D theoretical simulation and analysis of DC stray current corrosion (SCC) in oil fields that could contribute to oil leak and finally oil deferment of electric submersible pump (ESP) systems. Application of the boundary element analysis system (BEASY) allowed cathodic protection (CP) interference to be assessed in terms of the normal current density, which is directly proportional to the corrosion rate, rather than using the qualitative approach of measuring the potential shift of the soil. Different real structures consisting of pipelines and/or well casings with different arrangements and interference conditions are simulated. The results reveal that the application of impressed current cathodic protection (ICCP) systems creates DC SCC on other nearby unprotected structures. This is an inherent potential problem with the application of such systems which dominates with decreasing soil conductivity, and/or increasing the anode current density and its proximity to the protected structures. On the contrary, SCC can be reduced by using multi-groundbed anodes. Copyright © 2006 John Wiley & Sons, Ltd. [source] Organization of butyrate synthetic genes in human colonic bacteria: phylogenetic conservation and horizontal gene transferFEMS MICROBIOLOGY LETTERS, Issue 2 2007Petra Louis Abstract Butyrate producers constitute an important bacterial group in the human large intestine. Butyryl-CoA is formed from two molecules of acetyl-CoA in a process resembling ,-oxidation in reverse. Three different arrangements of the six genes coding for this pathway have been found in low mol% G+C-content Gram-positive human colonic bacteria using DNA sequencing and degenerate PCR. Gene arrangements were strongly conserved within phylogenetic groups defined by 16S rRNA gene sequence relationships. In the case of one of the genes, encoding ,-hydroxybutyryl-CoA dehydrogenase, however, sequence relationships were strongly suggestive of horizontal gene transfer between lineages. The newly identified gene for butyryl-CoA CoA-transferase, which performs the final step in butyrate formation in most known human colonic bacteria, was not closely linked to these central pathway genes. [source] Prediction of turbulent flow and heat transfer within rotating U-shaped passagesHEAT TRANSFER - ASIAN RESEARCH (FORMERLY HEAT TRANSFER-JAPANESE RESEARCH), Issue 6 2006Liu Chuan Kai Abstract Numerical predictions of three-dimensional flow and heat transfer are presented for rotating serpentine passages with and without rib turbulators. The coolant air is pressurized and its operating conditions are selected closely to match actual turbine operating parameters. Two different arrangements of rib turbulators were studied: (1) transverse ribs on the leading and trailing walls and (2) transverse ribs on all four walls. The rib height-to-hydraulic diameter ratio (e/Dh) is 0.143; the rib pitch-to-height ratio (s/e) is 7. Results for the rib-roughened serpentine passages were compared with those of smooth ones calculated in the literature. It was shown that a significant enhancement is achieved by means of rib turbulators in a serpentine passage at a stationary state as well as in a rotating state. In the radially-outward flow passages, the effect of rotation on heat transfer is relatively prominent. The secondary flows induced by the Coriolis forces are most intensive in the channel with four ribbed surfaces. The heat transfer after a 180° sharp turn in the smooth channel is influenced more by the sharp-turn-induced flow than the rib-roughened ones. © 2006 Wiley Periodicals, Inc. Heat Trans Asian Res, 35(6): 410,420, 2006; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/htj.20125 [source] Application of solid-phase concentration-dependent HSDM to the acid dye adsorption systemAICHE JOURNAL, Issue 1 2005Vinci K. C. Lee Abstract The fixed-bed adsorption of acid dyes onto granular activated carbon (Chemviron Filtrasorb 400) has been studied using a homogeneous surface diffusion model (HSDM). The model incorporates the external boundary layer mass transport and homogeneous diffusion inside the particle. A new orthogonal collocation method has been developed and used to solve the diffusion equations. This orthogonal collocation gives a faster solution method compared with the numerical Crank,Nicolson method. The surface diffusivity has been determined by an optimization procedure with minimization of sum of the error squared. The equilibrium relationship between the liquid-phase concentration and the solid-phase concentration has been described by the Redlich,Peterson isotherm. A solid-phase concentration-dependent surface diffusivity was introduced. The Darken model with the Redlich,Peterson isotherm was found to be a suitable correlation model for the adsorption of the acid dyes on carbon. The magnitude of the averaged Ds0 of each dye is in the order of AR114 > AB80 > AY117, which implies that, under the same solid-phase concentration gradient, the rate of mass transport diffusion is higher in AR114 than that in AB80 and AY117. This phenomenon may be explained by the different mobilities of the dye molecules present in the solution by the different arrangements of two sulfonic acid groups in the dye structures. © 2004 American Institute of Chemical Engineers AIChE J, 51: 323-332, 2005 [source] ULTRASTRUCTURAL CHARACTERIZATION OF THE LYTIC CYCLE OF AN INTRANUCLEAR VIRUS INFECTING THE DIATOM CHAETOCEROS CF. WIGHAMII(BACILLARIOPHYCEAE) FROM CHESAPEAKE BAY, USA,JOURNAL OF PHYCOLOGY, Issue 4 2009Yoanna Eissler Numerous microalgal species are infected by viruses that have the potential to control phytoplankton dynamics by reducing host populations, preventing bloom formation, or causing the collapse of blooms. Here we describe a virus infecting the diatom Chaetoceros cf. wighamii Brightw. from the Chesapeake Bay. To characterize the morphology and lytic cycle of this virus, we conducted a time-course experiment, sampling every 4 h over 72 h following viral inoculation. In vivo fluorescence began to decline 16 h after inoculation and was reduced to <19% of control cultures by the end of experiment. TEM confirmed infection within the first 8 h of inoculation, as indicated by the presence of virus-like particles (VLP) in the nuclei. VLP were present in two different arrangements: rod-like structures that appeared in cross-section as paracrystalline arrays of hexagonal-shaped profiles measuring 12 ± 2 nm in diameter and uniformly electron-dense hexagonal-shaped particles measuring , 22,28 nm in diameter. Nuclei containing paracrystalline arrays were most prevalent early in the infection cycle, while cells containing VLP increased and then declined toward the end of the cycle. The proportion of nuclei containing both paracrystalline arrays and VLP remained relatively constant. This pattern suggests that rod-like paracrystalline arrays fragmented to produce icosahedral VLP. C. cf. wighamii nuclear inclusion virus (CwNIV) is characterized by a high burst size (averaged 26,400 viruses per infected cell) and fast generation time that could have ecological implications on C. cf. wighamii population control. [source] z -Transform and adaptive signal processing in analysis of tracer dataTHE CANADIAN JOURNAL OF CHEMICAL ENGINEERING, Issue 3 2002Leszek Furman Abstract In the field of data processing, the common practice is to interpret tracer-determined residence time distributions (RTDs) of particles through different arrangements of elementary flow models. However, such analysis needs an a priori chosen arrangement of these models, and some information carried by the RTD curve may be lost. This paper presents a competitive method based on adaptive filtering in a z -transform domain, and it may give better insight into flow patterns in a steady-state flow system. A physical interpretation of the transfer function was developed. The application of this modelling to the interpretation of radiotracer data from recent studies in different industry sectors is presented. Dans le domaine du traitement des données, la pratique courante consiste à interpréter les distributions de temps de séjour (DTS) déterminées par traceur de particules à travers différents arrangements de modèles d'écoulements élémentaires. Toutefois, une telle analyse implique de choisir a priori l'arrangement de ces modèles. Ainsi, certaines informations données par la courbe de DTS peuvent se perdre. On présente dans cet article une méthode compétitive basée sur le filtrage adaptatif dans le domaine de la transformée en z, qui peut permettre une meilleure compréhension des schémas d'écoulement pour un système en écoulement permanent. Une interprétation physique de la fonction de transfert a été développée. On présente l'application de cette modélisation de l'interprétation des données de traceurs radioactifs provenant d'études récentes dans différents secteurs industriels. [source] Thermodynamic and spectroscopic study of the binding of dimethyltin(IV) by citrate at 25 °CAPPLIED ORGANOMETALLIC CHEMISTRY, Issue 7 2006Paola Cardiano Abstract Thermodynamic (potentiometric and calorimetric) and spectroscopic (1H NMR, 119Sn Mössbauer) studies were performed in aqueous solution in order to characterize the interaction of dimethyltin(IV) cation with citrate ligand. Six species {(CH3)2Sn(cit),; [(CH3)2Sn]2(cit)22,; (CH3)2Sn(cit)H0; (CH3)2Sn(cit)OH2,; [(CH3)2Sn]2(cit)OH0; [(CH3)2Sn]2(cit)(OH)2,} were found. All the species formed in this system are quite stable and formation percentages are fairly high. For example, at pH = 7.5 and C = Ccit = 10 mmol l,1, ,% for [(CH3)2Sn]2(cit)(OH)2, and (CH3)2Sn(cit)OH2, species reaches 65%. Overall thermodynamic parameters obtained show that the main contribution to stability is entropic in nature. Thermodynamic parameters were discussed in comparison with a simple tricarboxylate ligand (1,2,3-propanetricarboxylate). Two empirical relationships were derived from thermodynamic formation parameters. Spectroscopic results fully confirm the speciation model defined potentiometrically and show the mononuclear species to have an eq-(CH3)2Tbp structure with different arrangements around the metal, while for [(CH3)2Sn]2(cit)(OH)2, there are two different Sn(IV) environments, namely trans -(CH3)2 octahedral and cis -(CH3)2 Tbp. Copyright © 2005 John Wiley & Sons, Ltd. [source] Regional Integration in East Asia: Achievements and Future ProspectsASIAN ECONOMIC POLICY REVIEW, Issue 2 2006Hadi SOESASTRO Economic integration in East Asia has been largely market driven. Attempts in the late 1980s to establish an East Asian regional economic grouping failed to materialize for a number of reasons. The financial crisis in 1997,1998 has strengthened the realization of regional countries that they need to have some self-help mechanisms to overcome that crisis and to prevent future crises. This led to the development of several functional integration programs, including the network of bilateral swap arrangements known as the Chiang Mai Initiative. However, progress remains slow. The question that has arisen is how far these efforts need to be supported by institutional integration. Should the ASEAN Plus Three, the main regional cooperation process in East Asia involving the 10 South-East Asian countries plus China, Japan, and South Korea, be deepened institutionally? Meanwhile, the region has seen the establishment of a new process, the East Asia Summit, involving the above 13 countries plus Australia, India, and New Zealand. How will these different arrangements contribute to East Asia's economic dynamism and prosperity as well as peace and political stability? [source] | ||||||||||||||||