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Diffuse Positivity (diffuse + positivity)

Distribution by Scientific Domains
Medical Sciences100%

Selected Abstracts

Salivary duct carcinoma with neuroendocrine features: Report of a case with cytological and immunohistochemical study

DIAGNOSTIC CYTOPATHOLOGY, Issue 3 2004
Juan B. Laforga M.D.
Abstract We report a salivary duct carcinoma (SDC) of parotid gland in a 75-year-old male. Initially, it was studied by fine-needle aspiration, which disclosed features of malignancy consistent with a high-grade carcinoma. Histologically, the tumor showed typical features of SDC, predominantly with a solid and apocrine pattern. The aggressive behavior of this tumor was documented by facial palsy and the presence of 12 regional lymph node metastases. Immunohistochemical study showed positivity for cytokeratins (AE1/AE3), cytokeratin 7, GCDFP-15, C-erbB-2, Mib-1, topoisomerase II ,, p53, and androgen receptors. Diffuse positivity with chromogranin-A, synaptophysin, and Grimelius stains was also observed, suggesting endocrine features. Phosphotungstic acid hematoxylin, antimitochondrial antigen, progesterone and estrogen receptors, cytokeratin 20, and S-100 stains were negative. To our knowledge, this is the first case reported of SDC exhibiting neuroendocrine differentiation. Diagn. Cytopathol. 2004;31:189,192. © 2004 Wiley-Liss, Inc. [source]

Cytopathological diagnosis of adult retinoblastoma in a vitrectomy specimen,

DIAGNOSTIC CYTOPATHOLOGY, Issue 1 2010
Maria E. Orellana M.D.
Abstract Retinoblastoma (RB) is extremely rare in adults. We describe a case of RB diagnosed by cytology in a vitrectomy specimen of a 23-year-old patient who presented with diminished visual acuity and retinal detachment in the absence of a clinically-visible mass. Cytological examination of the vitreous fluid showed clusters of loosely cohesive atypical cells with high nuclear to cytoplasmic ratio and "salt and pepper" chromatin pattern in a background of normal neuronal retinal cells. Nuclear molding was present as well as numerous apoptotic bodies. The cells were focally positive for epithelial markers and showed strong and diffuse positivity for neuroendocrine markers. Ki-67 stained 90% of the "atypical cells" nuclei, in contrast to nonneoplastic retinal neuronal cells, which were negative for the marker. A diagnosis of RB was rendered, and subsequently was confirmed in the enucleation specimen. The cytological differential diagnosis is discussed as well as the role that cytology and immunohistochemistry can play in differentiating neoplastic cells from normal retinal cellular elements in vitreous fluid specimens. Diagn. Cytopathol. 2010. © 2009 Wiley-Liss, Inc. [source]

Cranial fasciitis of childhood

INTERNATIONAL JOURNAL OF DERMATOLOGY, Issue 2 2003
Margarita Larralde MD
A 2-month-old boy was seen at our pediatric dermatology department with a history of a tumoral lesion of the scalp since his birth. On examination he had a single, ovoid, firm, 2 × 1.8-cm painless subcutaneous mass on the temporal left calvarium, covered by normal skin (Fig. 1). It had experienced explosive growth in the preceding 2 weeks. There was no history of previous trauma in the area. The remainder of the examination was normal. Roentgenographic studies of the skull revealed a soft-tissue mass without involvement of the underlying bone. Ultrasonography of the lesion showed it to be an echolucid tumor. With the presumed diagnosis of dermoid cyst we sent the patient for surgical removal. At surgery, the lesion did not have the typical surgical appearance of a cyst. The histopathologic exam of the specimen was interpreted as cranial fasciitis of childhood (Fig. 2). Immunohistochemistry showed diffuse positivity for vimentin and muscle actin. After 1 year the patient is free of lesions. Figure 1. Lesion at the temporal left calvarium Figure 2. Proliferation of loosely arranged spindle cells in a loose myxoid stroma (H&E stain, × 40) [source]

Unusual recurrent orbital tumour

ACTA OPHTHALMOLOGICA, Issue 2007
LD IRION
Purpose: We present a case of an unusual CD34+ recurrent orbital tumour. Methods: A 62-year-old male presented with progressive left proptosis and preserved visual acuity. CT scans showed a circumscribed mass at the inferomedial orbit. After incisional biopsy, the patient preferred conservative treatment and the lesion was debulked. Further debulking was necessary in other two occasions. With radiological signs of bone invasion, left orbital exenteration was agreed in the fourth relapse. Results: The lid skin sparing exenteration revealed a tumour mass (45 x 29 x 27 mm) in the inferomedial orbit. There was no evidence of invasion of either the globe or the optic nerve. In all occasions, the tumour consisted of spindle cells alternating vague storiform areas with patternless areas. The tumour was very cellular, showed low mitotic count and no necrosis or ulceration. There was focal invasion of fibrous tissue, extra-ocular muscle, fat and bony fragments. The tumour was diffusely and consistently positive for CD34, S100 and vimentin. EMA and CD99 were focally positive. Several other markers were negative. This slow growing lesion with low grade histological appearance and EM suggestive of Schwannian processes was diagnosed as a CD34 positive Schwannoma on the first debulking. In the next two recurrences, experts agreed with a diagnosis of a DFSP based on the diffuse positivity for CD34. In the exenteration specimen, due to the exceptional location of the presumable DFSP, this diagnosis was disputed and after EM and further reviews the case was concluded as variant of low grade MPNST. Conclusions: The consistent positivity for CD34 in our case has lead to diagnostic disagreement. Only after the fourth recurrence the final diagnosis of a CD34 positive low grade PNST could be made. [source]