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Diel Changes (diel + change)
Selected AbstractsEXAMINATION OF DIEL CHANGES IN GLOBAL TRANSCRIPT ACCUMULATION IN SYNECHOCYSTIS (CYANOBACTERIA),JOURNAL OF PHYCOLOGY, Issue 3 2006Rochelle G. Labiosa Phytoplankton in nature must acclimate to a wide range of light conditions resulting from diel light cycles, ocean circulation and mixing, cloud cover, and the variable bio-optical characteristics of the water column. In this study, we used whole-genome cDNA microarrays to investigate the effects of a gradually fluctuating daily light cycle on gene expression in the cyanobacterium Synechocystis sp. strain PCC6803. From these data, we developed a conceptual framework depicting the diel regulation of metabolic pathways in the cell. The framework is focused on potential photoacclimation responses, including the regulation of the photosystems, cell division, and DNA replication. The mRNA abundance of genes involved in many metabolic pathways, and particularly those encoding proteins that function in photosynthesis and DNA replication, changed markedly over the course of the day. The levels of mRNA encoding polypeptides important for the formation of the light-harvesting apparatus, photosystems I and II, and cell division were found in high concentrations during the day. The transcript levels of many genes encoding enzymes involved in anabolic processes also increased considerably during the day. In contrast, transposon transcripts and mRNAs encoding proteins involved in DNA replication, cell wall synthesis, and respiratory activity were not found in high concentrations during the day. Although gradually varying light exposure induced significant changes in transcript accumulation within Synechocystis, the direction of these changes differed between our study and previous studies in which there was an abrupt transition between irradiances. [source] Diel Changes in Phytoplankton Composition and Abundance in the Surface and Sub-Surface Strata from a Shallow Eutrophic PondINTERNATIONAL REVIEW OF HYDROBIOLOGY, Issue 1 2009bieta Wilk-Wo, niak Abstract Representative phytoplankton assemblages were identified in a eutrophic pond over a 24 hour period. One assemblage characterized species in the surface (neuston) layer and another consisted of algae from 2, 5, and 20 cm sub-surface depths. The surface layer (0 cm) included a similar, but less diverse assemblage of species, and a lower abundance of cells per unit volume, than those at the lower depths. At each of the sub-surface depths (2,20 cm), the major phytoplankton components initially followed similar patterns of abundance in reference to the time and depth of sample collections then later differed in their abundance levels. The dominant algae were chlorophytes, cryptophytes, diatoms, and cyanobacteria. Mean concentrations of total phytoplankton, over the 24 hours for the surface, were 3.3 × 103 cells ml,1, compared to 36.9 × 103 cells ml,1 for depths 2,20 cm. The autotrophic picoplankton abundance was recorded separately from the phytoplankton with mean concentrations of 472.9 × 103 cells ml,1 in the surface layer and 623.0 × 103 cells ml,1 for the three sub-surface depths. Photos of representative species from these surface layers are presented. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) [source] MEASUREMENT OF IN SITU SPECIFIC GROWTH RATES OF MICROCYSTIS (CYANOBACTERIA) FROM THE FREQUENCY OF DIVIDING CELLS,JOURNAL OF PHYCOLOGY, Issue 5 2009Yoshimasa Yamamoto Diel changes in the frequency of dividing cells (FDC) of three Microcystis species were investigated in a small eutrophic pond from July to October 2005. The representative species was M. aeruginosa (Kütz.) Kütz., constituting 57%,86% of the Microcystis population throughout the study period, and the remainder were M. viridis (A. Braun) Lemmerm. and M. wesenbergii (Komárek) Komárek. The FDC of M. aeruginosa and M. wesenbergii increased in the daytime and fell in the nighttime in July and August, but this regular variation was not observed in September or October. The in situ specific growth rates of Microcystis species were estimated based on the assumption that the specific growth rate can be given as an absolute value of the derivative of FDC with respect to time. The calculated values were similar among species,0.15,0.38 · d,1 for M. aeruginosa, 0.14,0.63 · d,1 for M. viridis, and 0.18,0.61 · d,1 for M. wesenbergii. The specific growth rates in July and August slightly exceeded those in September and October. The analysis of the in situ specific growth rate of Microcystis indicated that recruitment of the benthic population or morphological change, rather than massive growth, was at least partly responsible for the dominance of M. aeruginosa in the study pond. [source] Sub-optimal morning temperature induces photoinhibition in dense outdoor cultures of the alga Monodus subterraneus (Eustigmatophyta)PLANT CELL & ENVIRONMENT, Issue 10 2001A. Vonshak Abstract Diel changes in photosynthetic oxygen evolution and several photochemical parameters measured by chlorophyll fluorescence quenching and induction were measured in outdoor dense cultures of the alga Monodus subterraneus (Eustigmatophyta). Cultures were maintained under two temperature regimes. In one, a rise in temperature was initiated in the morning by the increase in solar radiation up to the optimal temperature of 28 °C; in the other, a heating device was used to increase the rate of warming up in early morning. Although the two cultures were maintained at the same temperature and light intensity for most of the day, cultures exposed for only a short time to suboptimal morning temperature showed a larger decrease in almost all the photosynthetic parameters. By comparing the diel changes in maximal photochemistry efficiency of photosystem II, the electron transport rate and the photochemical and non-photochemical chlorophyll fluorescence quenching of the cultures, we concluded that even a relatively short exposure to suboptimal morning temperatures induced photoinhibitory damage. The higher photochemical activity of the heated culture was also reflected in a significant increase in productivity, which was 60% higher in the morning heated cultures than in the non-heated cultures. [source] The effects of diel changes in circulation and mixing on the longitudinal distribution of phytoplankton in a canyon-shaped Mediterranean reservoirFRESHWATER BIOLOGY, Issue 9 2010JAVIER VIDAL Summary 1. The near-surface distribution of phytoplankton cells along the thalweg of a canyon-shaped reservoir (El Gergal, southern Spain) during two surveys is described and interpreted as the result of time-varying large-scale circulation patterns, vertical mixing processes and the physiological capacity of algal cells to regulate its position in the water column. 2. Vertical gradients of chlorophyll-a concentration developed in the water column during the day but disappeared at night, as a result of the shoaling and deepening of the diurnal mixed layer (dml). The changes in the depth of the dml are largely controlled in El Gergal by convectively driven mixing processes. The longitudinal circulation changes, in turn, as a result of weak and diurnal land-sea breezes. The distribution of algal cells was patchy at all times but did not change during any of the surveys. 3. An expression is proposed to estimate time scales for the development of horizontal patchiness TP based on simple concepts of transport. It is shown that TP is in the order of a week, indicating that horizontal patchiness does not respond immediately to hourly changes in the controlling factors. The magnitude of TP, though, depends on how the vertical distribution of chlorophyll-a and longitudinal currents change on subdiurnal time scales. In particular, TP is sensitive to the lag existing between the momentum and heat fluxes through the free surface, driving circulation and vertical mixing. [source] DIEL VARIATIONS IN OPTICAL PROPERTIES OF IMANTONIA ROTUNDA (HAPTOPHYCEAE) AND THALASSIOSIRA PSEUDONANA (BACILLARIOPHYCEAE) EXPOSED TO DIFFERENT IRRADIANCE LEVELS,JOURNAL OF PHYCOLOGY, Issue 3 2008Sébastien Mas Diel variations of cellular optical properties were examined for cultures of the haptophyte Imantonia rotunda N. Reynolds and the diatom Thalassiosira pseudonana (Hust.) Hasle et Heimdal grown under a 14:10 light:dark (L:D) cycle and transferred from 100 ,mol photons · m,2 · s,1 to higher irradiances of 250 and 500 ,mol photons · m,2 · s,1. Cell volume and abundance, phytoplankton absorption coefficients, flow-cytometric light scattering and chl fluorescence, and pigment composition were measured every 2 h over a 24 h period. Results showed that cell division was more synchronous for I. rotunda than for T. pseudonana. Several variables exhibited diel variability with an amplitude >100%, notably mean cell volume for the haptophyte and photoprotective carotenoids for both species, while optical properties such as flow-cytometric scattering and chl a,specific phytoplankton absorption generally showed <50% diel variability. Increased irradiance induced changes in pigments (both species) and mean cell volume (for the diatom) and amplified diel variability for most variables. This increase in amplitude is larger for pigments (factor of 2 or more, notably for cellular photoprotective carotenoid content in I. rotunda and for photosynthetic pigments in T. pseudonana) than for optical properties (a factor of 1.5 for chl a,specific absorption, at 440 nm, in I. rotunda and a factor of 2 for the absorption cross-section and the chl a,specific scattering in T. pseudonana). Consequently, diel changes in optical properties and pigmentation associated with the L:D cycle and amplified by concurrent changes in irradiance likely contribute significantly to the variability in optical properties observed in biooptical field studies. [source] Seasonal and diel changes of dissolved oxygen in a hypertrophic prairie lakeLAKES & RESERVOIRS: RESEARCH AND MANAGEMENT, Issue 3 2005Richard D. Robarts Abstract Humboldt Lake, a hypertrophic prairie lake typical of many found on the Great Plains of North America, is usually ice-covered from early November to about mid-May. The lake is an important recreational fishery, now mainly stocked with walleye. It has a high potential risk of experiencing fish kills because of the very large cyanobacterial blooms that develop in it, the high rates of algal and bacterial production and the high concentrations of ammonia (NH3 -N) and dissolved organic matter. Following the collapse of cyanobacterial blooms, shallow prairie lakes are known to undergo periods of anoxia that can lead to summer fish kills. In some of the lakes, anoxia forms during the long period of ice cover, causing winter fish kills. Two years of seasonal and diel data (total phosphorus, dissolved oxygen (DO), NH3 -N and chlorophyll- a concentrations, and bacterial production) were analysed in this study to assess why significant fish kills did not occur during this period or during the , 30 years of records from Saskatchewan Environment. Humboldt Lake did not become anaerobic, either following the collapse of the cyanobacterial bloom or under ice cover, indicating that the oxygen (O2) influx (strong mixing) and production processes were greater than the microbial and chemical O2 demands, both over seasonal and diel time scales. Several published risk threshold criteria to predict the probability of summer and/or winter fish kills were applied in this study. The threshold criteria of maximum summer chlorophyll and maximum winter NH3 -N concentrations indicated that a summer fish kill was unlikely to occur in this hypertrophic prairie lake, provided its water quality remained similar to that during this study. Similarly, the threshold criteria of initial DO storage before ice cover and the rate of O2 depletion under ice cover also indicated a winter fish kill was unlikely. However, recent development in the watershed might have resulted in significant water quality deterioration and the winter fish kill that occurred in 2005. [source] Sub-optimal morning temperature induces photoinhibition in dense outdoor cultures of the alga Monodus subterraneus (Eustigmatophyta)PLANT CELL & ENVIRONMENT, Issue 10 2001A. Vonshak Abstract Diel changes in photosynthetic oxygen evolution and several photochemical parameters measured by chlorophyll fluorescence quenching and induction were measured in outdoor dense cultures of the alga Monodus subterraneus (Eustigmatophyta). Cultures were maintained under two temperature regimes. In one, a rise in temperature was initiated in the morning by the increase in solar radiation up to the optimal temperature of 28 °C; in the other, a heating device was used to increase the rate of warming up in early morning. Although the two cultures were maintained at the same temperature and light intensity for most of the day, cultures exposed for only a short time to suboptimal morning temperature showed a larger decrease in almost all the photosynthetic parameters. By comparing the diel changes in maximal photochemistry efficiency of photosystem II, the electron transport rate and the photochemical and non-photochemical chlorophyll fluorescence quenching of the cultures, we concluded that even a relatively short exposure to suboptimal morning temperatures induced photoinhibitory damage. The higher photochemical activity of the heated culture was also reflected in a significant increase in productivity, which was 60% higher in the morning heated cultures than in the non-heated cultures. [source] | ||||||||||