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Diauxic Lag (diauxic + lag)

Distribution by Scientific Domains
Life Sciences100%

Selected Abstracts

Determination of diauxic lag in continuous culture

BIOTECHNOLOGY & BIOENGINEERING, Issue 3 2008
Dong-Uk Lee
Abstract A procedure was developed to characterize diauxic lag of bacteria switching between electron acceptors in continuous culture. In this procedure, a virtual batch growth curve is developed by integrating the time-dependent net specific growth rates of bacteria observed under continuous flow conditions. The length of diauxic lag and the highest net specific growth rate following lag are conveniently estimated from the virtual batch curve. The procedure was found to give reproducible diauxic lag lengths and highest net specific growth rates when applied to experimental data from replicate continuous culture trials. Biotechnol. Bioeng. 2008;101: 642,646. © 2008 Wiley Periodicals, Inc. [source]

Effect of periplasmic nitrate reductase on diauxic lag of Paracoccus pantotrophus

BIOTECHNOLOGY PROGRESS, Issue 4 2009
Kiranmai Durvasula
Abstract Paracoccus pantotrophus expresses two nitrate reductases,membrane bound nitrate reductase (Nar) and periplasmic nitrate reductase (Nap). In growth experiments with two denitrifying species (Paracoccus pantotrophus and Alcaligenes eutrophus) that have both Nap and Nar and two species (Pseudomonas denitrificans and Pseudomonas fluorescens) with Nar only, it was found that diauxic lag is shorter for bacteria that express Nap. In P. pantotrophus, napEDABC encodes the periplasmic nitrate reductase. To analyze the effect of Nap on diauxic lag, the nap operon was deleted from P. pantotrophus. The growth experiments with nap, mutant resulted in increased diauxic lag when switched from aerobic to anoxic respiration, suggesting Nap is responsible for shorter lags and helps in adaptation to anoxic metabolism after transition from aerobic conditions. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009 [source]