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Dinucleotide Microsatellites (dinucleotide + microsatellite)
Selected AbstractsEighteen microsatellite loci in Salix arbutifolia (Salicaceae) and cross-species amplification in Salix and Populus speciesMOLECULAR ECOLOGY RESOURCES, Issue 4 2009TAKESHI HOSHIKAWA Abstract Salix arbutifolia is a riparian dioecious tree species that is of conservation concern in Japan because of its highly restricted distribution. Eighteen polymorphic loci of dinucleotide microsatellites were isolated and characterized. Among these, estimates of the expected heterozygosity ranged from 0.350 to 0.879. Cross-species amplification was successful at 9,13 loci among six Salix species and at three loci in one Populus species. [source] Polymorphic dinucleotide microsatellites in tongue sole (Cynoglossus semilaevis)MOLECULAR ECOLOGY RESOURCES, Issue 6 2007X. LIAO Abstract The tongue sole, Cynoglossus semilaevis, is a rare marine flatfish distributed in Chinese coastal waters. From a (GT)n -enriched genomic library, 57 microsatellites were isolated and characterized. Seventeen of these loci were polymorphic in a test population with alleles ranging from three to 13, and observed and expected heterozygosities from 0.1613 to 1.0000 and from 0.2126 to 0.8983, respectively. Five loci deviated from the Hardy,Weinberg equilibrium in the sampled population, and linkage disequilibrium between two loci was significant after applying Bonferroni correction. Three additional fish species assessed for cross-species amplification revealed that only one locus was also polymorphic in one species. These polymorphic microsatellite loci should provide sufficient level of genetic diversity to evaluate the breeding strategy and investigate the fine-scale population structure in C. semilaevis. [source] Isolation of dinucleotide microsatellite loci from red-backed salamander (Plethodon cinereus)MOLECULAR ECOLOGY RESOURCES, Issue 1 2003Lisa M. Connors Abstract We isolated 13 variable dinucleotide microsatellites from red-backed salamanders (Plethodon cinereus). After generating fragments using degenerate oligonucleotide primer-polymerase chain reaction (DOP-PCR), AC repeats were captured using biotinylated probes and streptavidin-coated magnetic particles. Captured fragments were cloned into plasmids, screened for microsatellites with a simple PCR reaction, and select plasmids then sequenced. PCR primers were designed and optimized for robust amplification, and nine primers have been further optimized for multiplex reactions with fluorescent primers. These nine loci are variable with an average of 6.11 alleles per locus and an average heterozygosity of 0.61. [source] Identification and characterization of 40 dinucleotide microsatellites in the dog genomeANIMAL GENETICS, Issue 5 2002Y. Ichikawa No abstract is available for this article. [source] | ||||||||||