Home About us Contact
|
Home About us Contact | ||
|
|
||
Development Rate (development + rate)
Selected AbstractsTemperature-dependent development and distribution in the soil profile of pupae of greyback canegrub Dermolepida albohirtum (Waterhouse) (Coleoptera: Scarabaeidae) in Queensland sugarcaneAUSTRALIAN JOURNAL OF ENTOMOLOGY, Issue 1 2007David P Logan Abstract, The temperature-dependent development rate of pupae of greyback canegrub, Dermolepida albohirtum (Waterhouse) (Coleoptera: Scarabaeidae), a major pest of sugarcane in central and northern Queensland, was determined under six constant temperature regimes: 18, 20, 23, 25, 27 and 30°C, and for four geographically separated populations. Development rate increased significantly with increasing temperature. Parameters of the linear regression equation did not differ among populations and common coefficients were calculated. Developmental zero, at and below which no development occurs, for pupae was 12.0°C and the thermal constant was 476 day-degrees (D°). Minimum and maximum periods for pupal development were 26 days at 30°C and 75 days at 18°C, respectively. The phenology of pupae was determined in soil-filled cubicles in a shade house and in the field at Ayr (19°35,S, 147°25,E), north Queensland, using D. albohirtum field-collected as late-stage third-instar larvae and kept in containers. Pupation of D. albohirtum began in late August or early September and eclosion was complete by mid- to late October. The phenology data were used to validate the development model. Eclosion was predicted by summing hourly fractions of day-degrees until 476 D° was reached and was close to actual eclosion. As temperature, and hence pupal development rate, varies with soil depth, the distribution of the third instars in pupal cells in the soil profile was determined in recently harvested fields of sugarcane in the Burdekin sugarcane district centred on Ayr. Numbers of late third instars in pupal cells peaked at 300,400 mm, with pupae found from 30 to 700 mm. Pupal development was simulated using hourly soil temperatures measured at depths of 200 and 400 mm at Ayr and at Sarina (21°22,S, 149°13,E). The pupal stage was predicted to take up to 2,10 days longer at 200 mm deep than at 400 mm depending on pupation site and date. When pupation was simulated in late August, as is likely in the field, pupal development at 400 mm deep took 48,56 days at Ayr and 58,62 days at Sarina. [source] Crowding and disease: effects of host density on response to infection in a butterfly,parasite interactionECOLOGICAL ENTOMOLOGY, Issue 5 2009ELIZABETH LINDSEY Abstract. 1. Hosts experiencing frequent variation in density are thought to benefit from allocating more resources to parasite defence when density is high (,density-dependent prophylaxis'). However, high density conditions can increase intra-specific competition and induce physiological stress, hence increasing host susceptibility to infection (,crowding-stress hypothesis'). 2. We studied monarch butterflies (Danaus plexippus) and quantified the effects of larval rearing density on susceptibility to the protozoan parasite Ophryocystis elektroscirrha. Larvae were inoculated with parasite spores and reared at three density treatments: low, moderate, and high. We examined the effects of larval density on parasite loads, host survival, development rates, body size, and wing melanism. 3. Results showed an increase in infection probability with greater larval density. Monarchs in the moderate and high density treatments also suffered the greatest negative effects of parasite infection on body size, development rate, and adult longevity. 4. We observed greater body sizes and shorter development times for monarchs reared at moderate densities, and this was true for both unparasitised and parasite-treated monarchs. We hypothesise that this effect could result from greater larval feeding rates at moderate densities, combined with greater physiological stress at the highest densities. 5. Although monarch larvae are assumed to occur at very low densities in the wild, an analysis of continent-wide monarch larval abundance data showed that larval densities can reach high levels in year-round resident populations and during the late phase of the breeding season. Treatment levels used in our experiment captured ecologically-relevant variation in larval density observed in the wild. [source] Selection for discontinuous life-history traits along a continuous thermal gradient in the butterfly Aricia agestisECOLOGICAL ENTOMOLOGY, Issue 6 2005Steve Burke Abstract., 1.,Voltinism may be conceptualised as the product of development rate and the timing of diapause , two components that together translate gradual environmental variation, through periods of growth and development, into ,generational units'. This may result in very different selection pressures on diapause induction and development time in populations with different numbers of generations per year. 2.,Developmental data from univoltine and bivoltine populations of the butterfly Aricia agestis (Lepidoptera: Lycaenidae) in North Wales were used to examine larval development time and the timing of diapause, and their contribution towards voltinism in populations that occur at the same latitude and in geographic proximity to one another along a thermal gradient. 3.,The critical photoperiod for diapause induction in univoltines and bivoltines from the same latitude differed by more than 1.5 h. 4.,Development time also differed significantly between these populations, in line with predictions that bivoltines would need to exhibit shorter development times in order to achieve two complete generations per year. Shorter development times for bivoltines result in lower pupal weights, suggesting a trade-off exists between generation number and body size that may dictate the position of the transition zone between the two life-history strategies. Analysis of development times in a third population, from southern England, with greater thermal availability than those from North Wales, further supports this hypothesised trade-off. 5.,To achieve the conversion of a continuous thermal gradient into the binary biological response from univoltism to bivoltism, bivoltines speed up development, reduce adult body size and shift their diapause induction response. [source] Scaling up evolutionary responses to elevated CO2: lessons from ArabidopsisECOLOGY LETTERS, Issue 5 2004Joy K. Ward Abstract Results from norm of reaction studies and selection experiments indicate that elevated CO2 will act as a selective agent on natural plant populations, especially for C3 species that are most sensitive to changes in atmospheric CO2 concentration. Evolutionary responses to CO2 may alter plant physiology, development rate, growth, and reproduction in ways that cannot be predicted from single generation studies. Moreover, ecological and evolutionary changes in plant communities will have a range of consequences at higher spatial scales and may cause substantial deviations from ecosystem level predictions based on short-term responses to elevated CO2. Therefore, steps need to be taken to identify the plant traits that are most likely to evolve at elevated CO2, and to understand how these changes may affect net primary productivity within ecosystems. These processes may range in scale from molecular and physiological changes that occur among genotypes at the individual and population levels, to changes in community- and ecosystem-level productivity that result from the integrative effects of different plant species evolving simultaneously. In this review, we (1) synthesize recent studies investigating the role of atmospheric CO2 as a selective agent on plants, (2) discuss possible control points during plant development that may change in response to selection at elevated CO2 with an emphasis at the primary molecular level, and (3) provide a quantitative framework for scaling the evolutionary effects of CO2 on plants in order to determine changes in community and ecosystem productivity. Furthermore, this review points out that studies integrating the effects of plant evolution in response to elevated CO2 are lacking, and therefore more attention needs be devoted to this issue among the global change research community. [source] LEARNING ABILITY AND LONGEVITY: A SYMMETRICAL EVOLUTIONARY TRADE-OFF IN DROSOPHILAEVOLUTION, Issue 6 2008Joep M. S. Burger Learning ability can be substantially improved by artificial selection in animals ranging from Drosophila to rats. Thus these species have not used their evolutionary potential with respect to learning ability, despite intuitively expected and experimentally demonstrated adaptive advantages of learning. This suggests that learning is costly, but this notion has rarely been tested. Here we report correlated responses of life-history traits to selection for improved learning in Drosophila melanogaster. Replicate populations selected for improved learning lived on average 15% shorter than the corresponding unselected control populations. They also showed a minor reduction in fecundity late in life and possibly a minor increase in dry adult mass. Selection for improved learning had no effect on egg-to-adult viability, development rate, or desiccation resistance. Because shortened longevity was the strongest correlated response to selection for improved learning, we also measured learning ability in another set of replicate populations that had been selected for extended longevity. In a classical olfactory conditioning assay, these long-lived flies showed an almost 40% reduction in learning ability early in life. This effect disappeared with age. Our results suggest a symmetrical evolutionary trade-off between learning ability and longevity in Drosophila. [source] Control of fledging age in Leach's Storm-Petrel, Oceanodroma leucorhoa: chick development and prefledging mass lossFUNCTIONAL ECOLOGY, Issue 1 2005R. A. MAUCK Summary 1Leach's Storm-Petrel (Oceanodroma leucorhoa) chicks accumulate fat through most of the nestling period, near the end of which they typically weigh 50,100% more than their parents. Much of this excess mass is lost abruptly prior to leaving the nest and fledging itself marks the termination of parental care. 2In this study, we evaluated the hypothesis that the onset of the prefledging mass loss period coincides with the end of the development period and the completion of structural growth. 3We characterized the pattern of prefledging mass loss and the timing of fledging with respect to a point (T), based on wing growth, at which structural development stops. Variation in T suggested that development rate is partially influenced by parental food provisioning, particularly early in the nestling period (age 16,20 days) just prior to the onset of rapid primary feather growth. 4The timing of mass loss and fledging in relation to T suggested that prefledging mass loss begins upon completion of wing growth and that fledging occurs after chicks have reached a mass compatible with sustained flight. Thus, the time to fledging is positively correlated with nestling mass at the end of the development period. 5The prefledging period of mass loss is a unique phase of anorexia placed between parental feeding and self-feeding in the life of the young petrel. [source] Effect of parental ageing on offspring developmental and survival attributes in an aphidophagous ladybird, Cheilomenes sexmaculataJOURNAL OF APPLIED ENTOMOLOGY, Issue 7 2009K. Singh Abstract The present study is the first attempt to investigate the effect of parental ageing of Cheilomenes sexmaculata (Fabricius) on total developmental period, developmental rate, adult weight on emergence, longevity, egg to adult survival and age-specific survivorship of the offspring. Young parents (10,20 day old) produced offspring with the shortest total developmental period, highest development rate, highest weight on emergence, greater longevity and highest survival. Age-specific survivorship of the offspring of younger parents declined later than the offspring of middle (30,40 day old) and old (50,60 day old) aged parents. This study would help in understanding the effect of parental ageing on future generations of predaceous ladybird beetles and would be helpful in designing mass multiplication programme of the bioagent, C. sexmaculata, in the laboratory. [source] The biology of Agriotes sordidus Illiger (Col., Elateridae)JOURNAL OF APPLIED ENTOMOLOGY, Issue 9-10 2004L. Furlan Abstract:, This paper describes the life cycle, including adult behaviour, oviposition, larval and pupal development rate of Agriotes sordidus Illiger. Each larva passed through up to eight to 13 instars. The larval size range of each instar was defined. Larvae need live vegetable tissues to survive and grow, otherwise most die within 40 days. Resistance to starvation increases with the age of the larvae (last instars can survive up to 1 year without food at 20°C). Each instar passes through three phases: mandible hardening and darkening, feeding, pre-moulting. The intense feeding (damaging plants) phase lasted <25% of the whole development time. They are poliphagous and the rate of larval development does not vary with host-plant type (maize, alfalfa). Provided sufficient soil moisture and food are present, larval development rate strongly depends on soil temperature. The duration of each instar increased with the age of the larvae. No larval growth was observed below 9°C. Under laboratory conditions the average heat sum (above a base of 9°C) required for development from egg to adult was about 3900 DD. Similar results were found in the rearing cages and in the open field. At the latitudes of the regions where this study was carried out (northern Italy, Veneto between 45°34,00,,N and 45°42,00,,N and central-south Italy, Molise, between 41°49,720,,N and 41°56,501,,N) the 6th instar (which normally is the first one passing 10 mm in length) is attained by September of the same oviposition year. Pupae can be found between the end of May and September mostly in the upper soil layer. Their transformation into adults took about 16 days at 25°C. Larvae of different stages overwintered by burrowing deep into the soil. Vertical migrations during the year are described: they depend mostly on soil temperatures from October to early spring. The adults overwintered and laid eggs in the subsequent spring. At lower latitudes or in warm seasons most of the population completed its life cycle (from egg to egg) in 24 months over three calendar years. At more northern latitudes, part (sometimes most) of the population completed the whole life cycle in about 36 months over four calendar years. [source] Co-variation between the intensity of behavioural manipulation and parasite development time in an acanthocephalan,amphipod systemJOURNAL OF EVOLUTIONARY BIOLOGY, Issue 10 2010N. FRANCESCHI Abstract Pomphorhynchus laevis, a fish acanthocephalan parasite, manipulates the behaviour of its gammarid intermediate host to increase its trophic transmission to the definitive host. However, the intensity of behavioural manipulation is variable between individual gammarids and between parasite populations. To elucidate causes of this variability, we compared the level of phototaxis alteration induced by different parasite sibships from one population, using experimental infections of Gammarus pulex by P. laevis. We used a naive gammarid population, and we carried out our experiments in two steps, during spring and winter. Moreover, we also investigated co-variation between phototaxis (at different stages of infection, ,young' and ,old cystacanth stage') and two other fitness-related traits, infectivity and development time. Three main parameters could explain the parasite intra-population variation in behavioural manipulation. The genetic variation, suggested by the differences between parasite families, was lower than the variation owing to an (unidentified) environmental factor. Moreover, a correlation was found between development rate and the intensity of behavioural change, the fastest growing parasites being unable to induce rapid phototaxis reversal. This suggests that parasites cannot optimize at the same time these two important parameters of their fitness, and this could explain a part of the variation observed in the wild. [source] Direct and correlated responses to selection for larval ethanol tolerance in Drosophila melanogasterJOURNAL OF EVOLUTIONARY BIOLOGY, Issue 2 2001J. D. Fry Ethanol is an important larval resource and toxin for natural Drosophila melanogaster populations, and ethanol tolerance is genetically variable within and among populations. If ethanol-tolerant genotypes have relatively low fitness in the absence of ethanol, as suggested by the results of an earlier study, genetic variation for ethanol tolerance could be maintained by variation in ethanol levels among breeding sites. I selected for ethanol tolerance in large laboratory populations by maintaining flies on ethanol-supplemented media. After 90 generations, the populations were compared with control populations in egg-to-adult survival and development rate on ethanol-supplemented and unsupplemented food. When compared on ethanol-supplemented food, the ethanol-selected populations had higher survival and faster development than the control populations, but on unsupplemented food, the populations did not differ in either trait. These results give no evidence for a ,trade-off' between the ability to survive and develop rapidly in the presence of ethanol and the ability to do so in its absence. The effect of physiological induction of ethanol tolerance by exposing eggs to ethanol was also investigated; exposing eggs to ethanol strongly increased subsequent larval survival on ethanol-supplemented food, but did not affect survival on regular food, and slowed development on both ethanol-supplemented and regular food, partly by delaying egg hatch. [source] Porcine Plasma Proteins as a Surimi Protease Inhibitor: Effects on Actomyosin GelationJOURNAL OF FOOD SCIENCE, Issue 4 2000W. Visessanguan ABSTRACT Effect of porcine plasma proteins (PPP) on thermal gelation of actomyosin in the presence and absence of fish proteinase was studied using a dynamic rheological test. Substantial decreases in development rate and magnitude of gel modulus were observed by the addition of proteinase to actomyosin gels. PPP was effective in protecting a myosin-heavy chain from proteolytic degradation, however, PPP itself interfered with the formation of actomyosin gel. Lower gel modulus was observed with actomyosin gels developed with higher concentrations of PPP added. Overall, PPP reversed the loss of gel modulus by the proteinase, however, the recovered gel modulus was only as high as those containing PPP only. These results implicated that, although PPP may revert autolytic activity in surimi, it interfaces with actomyosin gelation. [source] RHEOLOGICAL, TEXTURAL AND MICROSTRUCTURE ANALYSIS OF THE HIGH-METHOXY PECTIN/GELATIN MIXED SYSTEMSJOURNAL OF TEXTURE STUDIES, Issue 5 2007H. LIU ABSTRACT The aim of this research was to evaluate the interaction between high-methoxy pectin and gelatin in solution and mixed gel utilizing rheological, texture profile and microstructure analysis. The results showed that the phase separation and association might both exist depending on the ratio of pectin to gelatin in the mixed system. After the pectin addition to the mixed system, the gelation properties of gelatin were improved at suitable conditions. The structure development rate, gel hardness and gel brittleness of the pectin/gelatin mixed system were positively affected by the addition of pectin and from there to expand the application of the mixed system in food industry. PRACTICAL APPLICATIONS Structure-forming polysaccharides and protein provide desired functional properties to a wide range of foods such as gel confectionery products, milk products and fat replacers. As an academic paper, this article provided the information that high-methoxy (HM) pectin could prove the gelation properties of gelatin to corporate audiences. We could find that the ratio of HM pectin to gelatin, and the concentration of these two polymers, dictate the texture properties of the mixed system. The pectin addition to the gelatin system affected the structure development rate, which might save time when the products are processed in practice. The melting temperature was increased with the pectin addition to the gelatin system, which is favorable if it is to be kept under higher temperature. [source] Genotype and temperature influence pea aphid resistance to a fungal entomopathogenPHYSIOLOGICAL ENTOMOLOGY, Issue 2 2003David A. Stacey Abstract. The influence of temperature on life history traits of four Acyrthosiphon pisum clones was investigated, together with their resistance to one genotype of the fungal entomopathogen Erynia neoaphidis. There was no difference among aphid clones in development rate, but they did differ in fecundity. Both development rate and fecundity were influenced by temperature, but all clones showed similar responses to the changes in temperature (i.e. the interaction term was nonsignificant). However, there were significant differences among clones in susceptibility to the pathogen, and this was influenced by temperature. Furthermore, the clones differed in how temperature influenced susceptibility, with susceptibility rankings changing with temperature. Two clones showed changes in susceptibility which mirrored changes in the in vitro vegetative growth rate of E. neoaphidis at different temperatures, whereas two other clones differed considerably from this expected response. Such interactions between genotype and temperature may help maintain heritable variation in aphid susceptibility to fungal pathogen attack and have implications for our understanding of disease dynamics in natural populations. This study also highlights the difficulties of drawing conclusions about the efficacy of a biological control agent when only a restricted range of pest genotypes or environmental conditions are considered. [source] Effects of paternal cigarette smoking on testicular function, sperm fertilizing capacity, embryonic development, and blastocyst capacity for implantation in ratsANDROLOGIA, Issue 2 2004A. Kapawa Summary. We evaluated the effects of paternal smoking on testicular function, sperm fertilizing capacity, embryonic development, and blastocyst capacity for implantation. Rats of group A were exposed to cigarette smoke for 10 weeks. Rats of group B were exposed to the smoke of incense sticks for 10 weeks. Rats of group C served as a control group. Rats of group D were exposed to cigarette smoke for 7 weeks only. Experimental period was 10 weeks in all groups. At the end of the experimental period serum testosterone responses to human chorionic gonadotropin stimulation, andro-gen-binding protein activity in testicular cytosols, epididymal sperm motility, and oocyte fertilization rate, oocyte cleavage rate, and blastocyst development rate after in vitro fertilization (IVF) trials were significantly smaller in group A compared with groups B and C. In contrast, fertilization rate, cleavage rate, and blastocyst development rate after intracytoplasmic sperm injection (ICSI) procedures were not significantly different among groups A, B, C, and D. Both after IVF trials and ICSI techniques, the proportion of the alive offspring to the number of transferred oocytes was significantly smaller in group A than in groups B and C. Cigarette smoke-exposure results in a secretory deficiency of Leydig and Sertoli cells leading to an impaired epididymal sperm maturation process and diminished capacity of spermatozoa to penetrate oocytes. In addition paternal cigarette smoke exposure affects the embryonic ability for implantation. [source] Ingestion of Artemia nauplii by Chinese mitten crab Eriocheir sinensis zoea larvaeAQUACULTURE RESEARCH, Issue 8 2009Li-ying Sui Abstract A series of ingestion trials were conducted to determine the ingestion rate of Artemia nauplii by Eriocheir sinensis zoea larvae with increasing densities of Artemia and with or without rotifers as a co-feed. At each zoeal stage, 10 groups of 10 larvae were reared individually in glass beakers and fed with increasing densities of newly hatched Artemia nauplii (0.5, 2.5, 5, 10 and 20 individual (ind.) mL,1) with or without rotifers (15,25 ind. mL,1) as a co-feed. The average number of ingested Artemia was measured over 24 h. In addition, the average larval development rate (Larval Stage Index, LSI) over a longer period (time needed for the best treatment to reach 100% moult or metamorphosis to the next larval stage) was compared. The results showed that Artemia ingestion rate of E. sinensis larvae increased with increasing prey densities and larval development, and had a significantly negative correlation with rotifer consumption for all zoeal stages. Rotifers as an alternative prey significantly affected the intake of Artemia at early larval stages (Z1 and Z2) and promoted LSI at a lower Artemia density. Further experiments are needed to clarify the effect of prey density on survival and larval development when larvae are reared communally. [source] Temperature-dependent development and distribution in the soil profile of pupae of greyback canegrub Dermolepida albohirtum (Waterhouse) (Coleoptera: Scarabaeidae) in Queensland sugarcaneAUSTRALIAN JOURNAL OF ENTOMOLOGY, Issue 1 2007David P Logan Abstract, The temperature-dependent development rate of pupae of greyback canegrub, Dermolepida albohirtum (Waterhouse) (Coleoptera: Scarabaeidae), a major pest of sugarcane in central and northern Queensland, was determined under six constant temperature regimes: 18, 20, 23, 25, 27 and 30°C, and for four geographically separated populations. Development rate increased significantly with increasing temperature. Parameters of the linear regression equation did not differ among populations and common coefficients were calculated. Developmental zero, at and below which no development occurs, for pupae was 12.0°C and the thermal constant was 476 day-degrees (D°). Minimum and maximum periods for pupal development were 26 days at 30°C and 75 days at 18°C, respectively. The phenology of pupae was determined in soil-filled cubicles in a shade house and in the field at Ayr (19°35,S, 147°25,E), north Queensland, using D. albohirtum field-collected as late-stage third-instar larvae and kept in containers. Pupation of D. albohirtum began in late August or early September and eclosion was complete by mid- to late October. The phenology data were used to validate the development model. Eclosion was predicted by summing hourly fractions of day-degrees until 476 D° was reached and was close to actual eclosion. As temperature, and hence pupal development rate, varies with soil depth, the distribution of the third instars in pupal cells in the soil profile was determined in recently harvested fields of sugarcane in the Burdekin sugarcane district centred on Ayr. Numbers of late third instars in pupal cells peaked at 300,400 mm, with pupae found from 30 to 700 mm. Pupal development was simulated using hourly soil temperatures measured at depths of 200 and 400 mm at Ayr and at Sarina (21°22,S, 149°13,E). The pupal stage was predicted to take up to 2,10 days longer at 200 mm deep than at 400 mm depending on pupation site and date. When pupation was simulated in late August, as is likely in the field, pupal development at 400 mm deep took 48,56 days at Ayr and 58,62 days at Sarina. [source] Dysplastic nodules frequently develop into hepatocellular carcinoma in patients with chronic viral hepatitis and cirrhosisCANCER, Issue 3 2006Masahiro Kobayashi M.D. Abstract BACKGROUND Advances in imaging technology have enhanced the detection of small nodular lesions during the course of chronic liver disease. METHODS Between 1995 and 2002, the authors examined 154 consecutive patients with small hepatic nodules without hepatocellular carcinoma (HCC) over a median duration of 2.8 years. The median size of these nodules was 14 mm (range, 7,40 mm). The initial histopathologic diagnosis included high-grade dysplastic nodule (HGDN) (n = 13), low-grade dysplastic nodule (LGDN) (n = 42), and regenerative nodule (RN) (n = 99). RESULTS A total of 29 (18.8%) nodules developed into HCC during the observation period. Cumulative HCC development rates at the first, third, and fifth year were 46.2%, 61.5%, and 80.8% for HGDN; 2.6%, 30.2%, and 36.6% for LGDN; and 3.3%, 9.7%, and 12.4% for RN, respectively. The rate of HCC development was significantly higher in the HGDN group than for other types (P < 0.001). Multivariate analysis disclosed that histopathologic diagnosis (P < 0.001) and findings on computed tomographic arterial portography (CT-AP) (P = 0.004) were significantly associated with future HCC development. The hazard ratios of HGDN and LGDN were 16.8 (95% confidence interval [CI], 6.19,45.6) and 2.96 (95% CI, 1.20,7.31), respectively. A decrease in portal blood flow also showed a significantly high hazard ratio of 3.04 (95% CI, 1.42,6.50). Approximate annual development rate to HCC was 20% in patients with HGDN and 10% in LGDN. CONCLUSION HGDN should be considered a precancerous lesion when it appears during follow-up of chronic viral hepatitis or cirrhosis. Reduced portal blood flow in the nodule on computed tomography-AP is also an important predictor for development of hepatocellular carcinoma. Cancer 2006. © 2005 American Cancer Society. [source] Climatic adaptation in an isolated and genetically impoverished amphibian populationECOGRAPHY, Issue 4 2010Germán Orizaola The capacity of populations to respond adaptively to environmental change is essential for their persistence. Isolated populations often harbour reduced genetic variation, which is predicted to decrease adaptive potential, and can be detrimental under the current scenarios of global change. In this study, we examined climatic adaptation in larval life history traits in the pool frog Rana lessonae along a latitudinal gradient across the northern distribution area of the species, paying special attention to the isolated and genetically impoverished fringe populations in central Sweden. Larvae from eight populations within three geographic areas (Poland, Latvia and Sweden) were reared under three temperatures (19, 22 and 26°C) in a common garden laboratory experiment. We found clear evidence for latitudinal adaptation in R. lessonae populations, larvae from higher latitudes growing and developing faster than low-latitude ones. Larvae from the Swedish populations were able to compensate for the effects of cooler temperatures and a shorter growth season with genetically higher growth and development rates (i.e. countergradient variation) in the two higher temperature treatments, but there was no difference among the populations at the lowest temperature treatment, which is likely to be close to the temperature limiting growth in R. lessonae. Our results demonstrate that isolated and genetically impoverished populations can be locally adapted, and identify the Swedish fringe populations as a significant conservation unit adapted to the northern environmental conditions. [source] Crowding and disease: effects of host density on response to infection in a butterfly,parasite interactionECOLOGICAL ENTOMOLOGY, Issue 5 2009ELIZABETH LINDSEY Abstract. 1. Hosts experiencing frequent variation in density are thought to benefit from allocating more resources to parasite defence when density is high (,density-dependent prophylaxis'). However, high density conditions can increase intra-specific competition and induce physiological stress, hence increasing host susceptibility to infection (,crowding-stress hypothesis'). 2. We studied monarch butterflies (Danaus plexippus) and quantified the effects of larval rearing density on susceptibility to the protozoan parasite Ophryocystis elektroscirrha. Larvae were inoculated with parasite spores and reared at three density treatments: low, moderate, and high. We examined the effects of larval density on parasite loads, host survival, development rates, body size, and wing melanism. 3. Results showed an increase in infection probability with greater larval density. Monarchs in the moderate and high density treatments also suffered the greatest negative effects of parasite infection on body size, development rate, and adult longevity. 4. We observed greater body sizes and shorter development times for monarchs reared at moderate densities, and this was true for both unparasitised and parasite-treated monarchs. We hypothesise that this effect could result from greater larval feeding rates at moderate densities, combined with greater physiological stress at the highest densities. 5. Although monarch larvae are assumed to occur at very low densities in the wild, an analysis of continent-wide monarch larval abundance data showed that larval densities can reach high levels in year-round resident populations and during the late phase of the breeding season. Treatment levels used in our experiment captured ecologically-relevant variation in larval density observed in the wild. [source] Effects of the synthetic estrogen ethinylestradiol on early life stages of mink frogs and green frogs in the wild and in situENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 8 2005Bradley J. Park Abstract Estrogenic contaminants are known to disrupt growth and development in amphibians. Field-based research is needed to elucidate their potential impacts on wild populations. Hatch success, larval growth and development rates, and gonad development were examined in native amphibians exposed to low ng/L concentrations of 17,-efhinylestradiol (EE2) in a whole-lake addition experiment at the Experimental Lakes Area, northwestern Ontario, Canada. Egg masses were reared in situ in the EE2-amended lake and in two reference lakes in 2001 and 2002. Hatching success was reduced significantly in green frogs (Rana clamitans) but not in mink frogs (Rana septentrionalis) exposed to EE2. Ethinylestradiol had no consistent effect on mass or development stage of hatchlings in the early larval stages of the caging study. Ethinylestradiol had no effect on sex ratios of either species in situ, and no intersex gonads were observed in exposed or reference green frog tadpoles or in reference mink frog tadpoles. However, 5.6% (total n = 18) and 12.5% (total n = 56) of EE2-exposed mink frog tadpoles were intersex in the 2001 and 2002 caging studies, respectively. Wild mink frog tadpoles also were examined, and EE2 had no effect on sex ratios. No intersex gonads were observed in reference lake tadpoles or in tadpoles from the experimental lake prior to EE2 additions; however, 2.4, 0, and 28.6% of wild EE2-exposed first-year tadpoles had intersex gonads (2001, 2002, and 2003, respectively). These results indicate that exposure to EE2 in the wild and in situ at concentrations comparable to those detected in effluents and, occasionally, in surface waters can impact gonad development and hatch success in native amphibians. [source] Microgeographic life history variation in an alpine caddisfly: plasticity in response to seasonal time constraintsFRESHWATER BIOLOGY, Issue 1 2009LISA N. S. SHAMA Summary 1.,Temporally constrained environments, such as habitats with short growth seasons or short hydroperiods, cause potentially strong selection on life histories. Depending on the predictability of these events and the extent of spatial and temporal heterogeneity, local populations could become adapted either via a fixed phenotype or via life history plasticity in response to these environmental cues. 2.,We used a common garden experiment to investigate microgeographic variation in life history responses to combined changes in photoperiod (ambient/late) and hydroperiod (constant/drying) time constraint cues in an alpine caddisfly (Trichoptera). We compared six populations (three permanent/three temporary streams) originating from a small, alpine floodplain and which spanned an expected gradient in growth period duration (GPD) with distance from glaciers. 3.,We made two main predictions in relation to locally varying selection pressures: (i) populations nearest glaciers (shorter GPD and strongest time constraints) should have the fastest development rates and (ii) populations from permanent streams should be less able to respond to drying hydroperiods than populations from temporary streams. 4.,All populations and both sexes accelerated development in response to late season photoperiod cues. However, only permanent stream populations showed an increase in development time with increasing GPD, suggesting that other factors were influencing populations in temporary streams. 5.,Permanent stream populations showed countergradient variation (genetic and environmental influences were in opposition) in development time, and under-compensation of growth rates resulted in a converse Bergmann cline in body size (smaller body size along gradients of declining season length). The extent of plasticity in response to hydroperiod, and the combined effects of both time constraints, differed between populations and sexes, but were not consistent among populations. 6.,Taken together, our results suggest adaptive plasticity in response to season length. The lack of a predictable pattern in response to hydroperiod may be due to gene flow or weak selection. We conclude that spatially structured populations can strongly differ in phenotypic plasticity even at microgeographic scales. [source] EFFECTS OF TEMPERATURE ON CERTAIN POPULATION PARAMETERS OF LIRIOMYZA SATIVAE BLAN-CHARD (DIPTERA: AGROMYZIDAE)INSECT SCIENCE, Issue 2 2000ZHANG Run-jie Abstract, Effects of temperature on population parameters and the intrinsic rate of natural increase of the leafminer, Liriomyza sativae Blanchard, were studied at constant temperatures, 15, 20, 25, 30 and 3593 80% RH and a photoperiod of 12:12 (L:D) in the laboratory with Phaseolus vulgaris as the host plant. Developmental time of the immature stage decreased from 38 d at 15C to lld at 3593 Regression equations relating temperature (t) to development rates (y) for egg, larval and pupal stages, were y = 1.7862t - 13.841, y = 1.162t - 4.946 and y= 0.634t - 5.146, respectively. Longevity of female adult decreased from 20 d at 15°C to 9 d with temperature up to 35C The most favorable temperature range for reproduction was 20°C - 30oC in which the fecundity ranged from 158 to 282 eggs per female. The lowest total mortality was 9% at 25oC and the highest was 49% at 35 93 High intrinsic rate of natural increase (rm) was 0.27 and high net reproductive rate (R0) was 116.8 at temperature range between 25 t and 30 t indicating that this range was optimal for population growth and that population density might increase 117 times per generation under this temperature condition. Mean generation time (T) and time for population to double (t) decreased as temperature increased, showing a negative linear trend with temperature. The relationship between finite rate of increase (A) and temperature, however, was a positive linear regression. [source] Utilisation of morphological features in life table studies of Liriomyza huidobrensis (Dipt., Agromyzidae) developing in lettuceJOURNAL OF APPLIED ENTOMOLOGY, Issue 7-8 2002J. Head The growth ratios of cephalopharyngeal skeletons between first and second and second and third instar larvae were 1.80 and 1.47, respectively, enabling clear separation to be achieved for experimental work. Using this method the development rates of the immature stages of L. huidobrensis feeding on Lactuca sativa were determined under constant temperatures of 11, 16, 19, 26 and 28 ± 1°C and were shown to increase linearly with temperature over the range investigated. The theoretical lower threshold temperatures for development from oviposition to the end of each larval instar or pupal stage were 5.35, 6.30, 6.20 and 5.70°C, respectively. The overall threshold temperature for development from oviposition to 50% adult emergence (5.70°C) was used to calculate degree-day (DD) requirements for development from oviposition to each larval instar or pupal eclosion, which were 84.3, 30.1, 58.9, 143.7 DD, respectively. The use of these data for optimizing the timing of application of control agents which are effective against specific developmental stages is discussed. [source] Protected raspberry production accelerates onset of oviposition by vine weevils (Otiorhynchus sulcatus)AGRICULTURAL AND FOREST ENTOMOLOGY, Issue 3 2010Scott N. Johnson 1Soft fruit production is increasingly reliant on crops that are grown under the protection of plastic tunnels, which may also affect insect communities as a result of localized climate change and changes to host plant physiology and chemistry. In particular, insect development rates may differ from field populations, making it more difficult to target control measures. 2The present study investigated how protected environments affected adult vine weevil (Otiorhynchus sulcatus) feeding and reproduction on red raspberry (Rubus idaeus). We focused on the period between adult emergence and the onset of oviposition (i.e. the pre-reproductive period), which represents the optimal period for control. 3Tunnels were up to 4 °C warmer than field plantations in 2008, with plants growing significantly faster (50% increase in height and 16% increase in leaf area) than field grown plants. The carbon/nitrogen ratio in leaves was higher in tunnels (12.07) than the field (10.89) as a result of a significant decrease in nitrogen concentrations (3.40 and 3.90 mg g,1, respectively). 4Over 4 weeks, weevils consumed significantly more foliage in tunnels (370.89 mg) than weevils in the field (166.68 mg), suggesting compensatory feeding to counteract lower leaf nitrogen concentrations. Weevils in tunnels achieved sexual maturity 8 days earlier than those in the field and produced 20-fold more eggs by the time they were 5 weeks old. 5Applying a degree-day model showed good agreement between predicted and observed pre-reproductive periods for weevils in tunnels (36 and 30 days, respectively) and in field plots (41 and 38 days, respectively). [source] The effects of 1.5T magnetic resonance imaging on early murine in-vitro embryo developmentJOURNAL OF MAGNETIC RESONANCE IMAGING, Issue 3 2001MMed (O&G), MRACOG, Stephen Chew MBBS Abstract Although no ionizing radiation is involved, patients undergoing magnetic resonance imaging (MRI) are exposed to powerful static magnetic fields, magnetic field gradients, and radio-frequency fields that may be potentially damaging. Our study aims to document the effect of MRI imaging sequences on early murine embryo development (two-cell to blastocyst stage) in vitro. Two-cell murine embryos were exposed to various lengths of MRI using pulse sequences employed in present day clinical imaging. Early murine embryo development was documented in vitro, and blastocyst development rates were computed for both the control and exposed groups. There were no significant differences detected in the rate of blastocyst formation between the control groups and the embryos exposed to MRI. J. Magn. Reson. Imaging 2001;13:417,420. © 2001 Wiley-Liss, Inc. [source] Seasonal variations in production and development of leather leaf fern leaves,ANNALS OF APPLIED BIOLOGY, Issue 2 2003J O STRANDBERG Summary Production and development of leatherleaf fern leaves were studied using containerised plants growing in a shade house. Leaf development was partitioned into seven arbitrary development stages; the time needed for leaves to progress through each developmental stage was used to measure days required to reach maturity. Measurements were made twice each week throughout 1997. Means and variances with in emergence rates of new leaves and time needed for new leaves to pass through six leaf development stages were calculated for 26 14-day periods throughout the year and compared with degree days, solar radiation, soil temperature, and daylight hours accumulated during the periods. Leaf production rates ranged from 0.15,0.73 leaves day,1 plant,1. The average time from emergence to maturity was 22.6 days. Both leaf production and development rates varied greatly with the seasons, and were strongly associated with the weather variables measured, but significant cyclic rate fluctuations unrelated to weather were also detected. Leaf emergence rates were more strongly related to average soil temperature, whereas leaf development rates to maturity were more strongly related to solar radiation and degree days. [source] Physiological variation along a geographical gradient: is growth rate correlated with routine metabolic rate in Rana temporaria tadpoles?BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY, Issue 1 2009BEATRICE LINDGREN Shorter season length and lower temperature towards higher latitudes and altitudes often select for intraspecific clines in development and growth rates. However, the physiological mechanisms enabling these clines are not well understood. We studied the relationship between routine metabolic rate (RMR) and larval life-history traits along a 1500-km latitudinal gradient across Sweden. In a laboratory common garden experiment, we exposed eight common frog Rana temporaria populations to two experimental temperatures (15 and 18 °C) and measured RMR using flow-through respirometry. We found significant differences among populations in RMR, but there was no evidence for a linear relationship between latitude and RMR in either temperature treatment. However, we found a concave relationship between latitude and RMR at the lower experimental temperature. RMR was not correlated with growth rate at population or at individual levels. The results obtained suggest that, unlike in growth and development rates, there is no latitudinal cline in RMR in R. temporaria tadpoles. © 2009 The Linnean Society of London, Biological Journal of the Linnean Society, 2009, 98, 217,224. [source] Dysplastic nodules frequently develop into hepatocellular carcinoma in patients with chronic viral hepatitis and cirrhosisCANCER, Issue 3 2006Masahiro Kobayashi M.D. Abstract BACKGROUND Advances in imaging technology have enhanced the detection of small nodular lesions during the course of chronic liver disease. METHODS Between 1995 and 2002, the authors examined 154 consecutive patients with small hepatic nodules without hepatocellular carcinoma (HCC) over a median duration of 2.8 years. The median size of these nodules was 14 mm (range, 7,40 mm). The initial histopathologic diagnosis included high-grade dysplastic nodule (HGDN) (n = 13), low-grade dysplastic nodule (LGDN) (n = 42), and regenerative nodule (RN) (n = 99). RESULTS A total of 29 (18.8%) nodules developed into HCC during the observation period. Cumulative HCC development rates at the first, third, and fifth year were 46.2%, 61.5%, and 80.8% for HGDN; 2.6%, 30.2%, and 36.6% for LGDN; and 3.3%, 9.7%, and 12.4% for RN, respectively. The rate of HCC development was significantly higher in the HGDN group than for other types (P < 0.001). Multivariate analysis disclosed that histopathologic diagnosis (P < 0.001) and findings on computed tomographic arterial portography (CT-AP) (P = 0.004) were significantly associated with future HCC development. The hazard ratios of HGDN and LGDN were 16.8 (95% confidence interval [CI], 6.19,45.6) and 2.96 (95% CI, 1.20,7.31), respectively. A decrease in portal blood flow also showed a significantly high hazard ratio of 3.04 (95% CI, 1.42,6.50). Approximate annual development rate to HCC was 20% in patients with HGDN and 10% in LGDN. CONCLUSION HGDN should be considered a precancerous lesion when it appears during follow-up of chronic viral hepatitis or cirrhosis. Reduced portal blood flow in the nodule on computed tomography-AP is also an important predictor for development of hepatocellular carcinoma. Cancer 2006. © 2005 American Cancer Society. [source] | ||||||||||||||||||||||||||||||||||