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Terms modified by Derived Selected AbstractsMR determination of glomerular filtration rate in subjects with solitary kidneys in comparison to clinical standards of renal function: feasibility and preliminary report,CONTRAST MEDIA & MOLECULAR IMAGING, Issue 2 2009Richard W. Katzberg Abstract This study was conducted to demonstrate the feasibility of quantifying single kidney glomerular filtration rate (skGFR) by magnetic resonance (MR) by comparison to the clinical estimates of GFR in volunteer subjects with a single kidney. Seven IRB-approved subjects with a solitary kidney, stable serum creatinine (SCr) and a 24,h creatinine clearance (CrCl) volunteered to undergo an MR examination that determined renal extraction fraction (EF) with a breathhold inversion recovery echo planar pulse sequence and renal blood flow with a velocity encoded phase imaging sequence. The product of EF and blood flow determines GFR. These values were compared with the 24,h CrCl, estimated GFR by the modification of diet in renal disease (MDRD) regression analysis and the Cockroft,Gault (CG) determination of CrCl. The mean and standard deviation of differences between the MR GFR, MDRD and CG vs the 24,h CrCl were 12.3,±,35.7, ,8.9,±,18.5 and 1.2,±,19.6, respectively. The Student t -test showed that none of the mean differences were statistically significant between techniques. This clinical investigation shows that MR can be used for skGFR determination in human subjects with comparable values to those derived from clinically used serum-based GFR estimation techniques. Copyright © 2009 John Wiley & Sons, Ltd. [source] Managing Risks in Multiple Online Auctions: An Options Approach,DECISION SCIENCES, Issue 3 2005Ram Gopal ABSTRACT The scenario of established business sellers utilizing online auction markets to reach consumers and sell new products is becoming increasingly common. We propose a class of risk management tools, loosely based on the concept of financial options that can be employed by such sellers. While conceptually similar to options in financial markets, we empirically demonstrate that option instruments within auction markets cannot be developed employing similar methodologies, because the fundamental tenets of extant option pricing models do not hold within online auction markets. We provide a framework to analyze the value proposition of options to potential sellers, option-holder behavior implications on auction processes, and seller strategies to write and price options that maximize potential revenues. We then develop an approach that enables a seller to assess the demand for options under different option price and volume scenarios. We compare option prices derived from our approach with those derived from the Black-Scholes model (Black & Scholes, 1973) and discuss the implications of the price differences. Experiments based on actual auction data suggest that options can provide significant benefits under a variety of option-holder behavioral patterns. [source] Psychometric evaluation of a measure of Beck's negative cognitive triad for youth: applications for African,American and Caucasian adolescentsDEPRESSION AND ANXIETY, Issue 4 2005Leilani Greening Abstract A measure of Beck's negative cognitive triad, the Cognitive Triad for Children (CTI-C), was evaluated for its psychometric properties and utility with a community sample of 880 African,American and Caucasian adolescents. High-school students ranging from 14 to 17 years of age completed the CTI-C, the Children's Depression Inventory (CDI) and the Children's Attributional Style Questionnaire-Revised (CASQ-R) on two occasions 4 months apart. The CTI-C was found to be internally consistent, Cronbach's ,=.90, to have acceptable test-retest reliability, r=.70, and concurrent validity as demonstrated by a significant correlation with the CASQ-R, r=.53. A principal factor analysis with promax rotation did not yield support for Beck's tripartite model of negative cognitions about the self, world, and future but rather yielded three factors with a combination of cognitions from all three domains. African American adolescents who reported more maladaptive cognitions on the CTI-C reported fewer depressive symptoms on the CDI 4 months later compared to their Caucasian counterparts, suggesting some limitation to using the CTI-C to predict depressive symptoms in African,American youth; however, Factor 1 derived from a factor analysis with the sample was more consistent in predicting future symptoms among both African,American and Caucasian adolescents. This factor consisted largely of positively worded items, offering some support for low positive affect as a predictor of depressive symptoms in adolescents. Depression and Anxiety 21:161,169, 2005. © 2005 Wiley-Liss, Inc. [source] Primary mesenchyme cell-ring pattern formation in 2D-embryos of the sea urchinDEVELOPMENT GROWTH & DIFFERENTIATION, Issue 1 2000Hideki Katow Primary mesenchyme cell (PMC) migration during PMC-ring pattern formation was analyzed using computer-assisted time-lapse video microscopy in spread embryos (2D-embryo) of the sea urchin, Mespilia globulus, and a computer simulation. The PMC formed a near normal ring pattern in the 2D-embryos, which were shown to be an excellent model for the examination of cell behavior in vivo by time-lapse computer analysis. The average migration distance of the ventro-lateral PMC aggregate-forming cells (AFC) and that of the dorso-ventral PMC cable-forming cells (CFC) showed no significant difference. All PMC took a rather straightforward migration path to their destinations with little lag time after ingression. This in vivo cell behavior fitted well to a computer simulation with a non-diffusable chemotaxis factor in the cyber-cell migration field. This simulation suggests that PMC recognize their destination from a very early moment of cell migration from the vegetal plate, and implicates that a chemoattractive region is necessary for making the PMC migration pattern. The left- and right-lateral AFC and dorso and ventral CFC were each derived from an unequally divided one-quarter segment of the vegetal plate. This suggests that AFC and CFC have a distinctive ancestor in the vegetal plate, and the PMC are a heterogeneous population at least in terms of their destination in the PMC-ring pattern. [source] Osteoderm morphology in recent and fossil euphractine xenarthransACTA ZOOLOGICA, Issue 4 2009C. M. Krmpotic Abstract The presence of osteoderms within the integument, forming a carapace, is one of the most distinctive features of armadillos with the external morphology of these elements forming the basis of most systematic schemes. This is especially true for fossil taxa, where these elements are most frequent in the palaeontological record. A detailed study of osteoderms from the cephalic shield and different regions of the dorsal armour of Chaetophractus villosus (Euphractinae, Xenarthra) was made and compared to those of the extant genus Dasypus (Dasypodinae, Xenarthra), and the extinct genus ,Eutatus. Three distinct histological zones were recognized: outer and inner zones are thin, formed by regular compact bone, the middle zone is thicker, with large cavities that contain mainly adipose tissue, hair follicles, and sweat and sebaceous glands. The internal structure of ,Eutatus (also a member of Euphractinae) osteoderms is close to that of C. villosus, consistent with the notion that these taxa are phylogenetically closely related. In contrast, Dasypus shows marked differences. Dasypus shows hair follicles associated with both gland types (sweat and sebaceous) and connected to foramina on the external surface. Although not observed in adult C. villosus, it has been documented during embryonic development, only to atrophy later in ontogeny. Furthermore, the presence of red bone marrow is rare in C. villosus, but widespread in Dasypus novemcinctus osteoderms. These results suggest an early split of both subfamilies and support the hypothesis that the Euphractinae are more derived than the Dasypodinae. [source] The humerus of Panderichthys in three dimensions and its significance in the context of the fish,tetrapod transitionACTA ZOOLOGICA, Issue 2009Catherine A. Boisvert Abstract The humerus of Panderichthys has been considered to represent a transitional form between that of tetrapodomorph fish such as Eusthenopteron and tetrapods such as Acanthostega. The previous description was based on flattened material and was analysed in the context of the few fossils known at the time. Since then, several new forms have been described such as Gogonasus, Tiktaalik and an isolated humerus from the Catskill Formation. The humeral morphology of Panderichthys rhombolepis and its interpretation in this new context are therefore reassessed with the help of a three-dimensional model produced with the mimics software based on a computed tomography scan of an unflattened specimen as well as comparisons with the originally described material. The humerus of Panderichthys displays a combination of primitive, derived, intermediate and unique characteristics. It is very similar to the morphology of Tiktaalik but when it differs from it, it is most often more derived despite the more basal phylogenetic position that Panderichthys occupies. What emerges from this study is a much more gradual transformation of the humerus morphology from fish to tetrapods and the ability to distinguish autapomorphies more easily. The picture is more complex than previously believed, with many morphological specializations probably reflecting the breadth of ecological specializations already present at the time. [source] Laboratory simulation of clast abrasionEARTH SURFACE PROCESSES AND LANDFORMS, Issue 2 2002J. Lewin Abstract Experimental abrasion of river-bed materials has been widely undertaken, producing ,downstream' fining rates that generally are believed to be much less than those observed in the field. A conclusion commonly adopted has been that sorting processes are more effective than abrasion processes. A comparative evaluation of results from an abrasion tank and a tumbling barrel are presented, which show that abrasion patterns and rates differ according to the equipment used, clast size and shape, the clast charge (barrel) and water velocity and bed material (tank). Abrasion is a composite process, and the effects achieved appear to be dominated by percussion in the tank and grinding in the barrel. Breakage, crushing and sandblasting are not modelled effectively, nor are effects achieved on clasts when they form part of the bed. Comparisons are made with other equipment used, the very limited amount of direct field abrasion monitoring, and with the probable suite of processes that may occur under field conditions. Also reviewed are the problems that arise when laboratory weight-loss abrasion coefficients are used or converted into ones of size diminution, as usually derived from field observations of down-channel trends. It is concluded that field abrasion rates generally have been underestimated, as the processes involved are at best only selectively represented by the experimental equipment so far used, and because the results obtained experimentally are capable of misinterpretation when related to field trends. Copyright © 2002 John Wiley & Sons, Ltd. [source] Nutrients, diversity, and community structure of two phytotelm systems in a lower montane forest, Puerto RicoECOLOGICAL ENTOMOLOGY, Issue 3 2000Barbara A. Richardson Summary 1. Bromeliad and heliconia phytotelmata in the same forest area were compared in terms of their animal assemblages, nutrient inputs, and plant architecture. 2. For all major elements, nutrient inputs from canopy-derived debris and rainfall in bromeliads were significantly lower than those derived from decaying flower parts and plant secretions in heliconia bracts. Bromeliads contained significantly fewer organisms per unit volume of water and unit dry weight of organic matter than did heliconia inflorescences. They also contained a significantly lower animal biomass (199 mg DW from 15 bromeliads, 527 mg DW from 15 heliconia inflorescences). 3. Species richness was independent of abundance, demonstrating that, at least for small container habitats, higher abundance does not necessarily lead to a greater species richness. Communities were remarkably similar in patterns of relative abundance and species richness (23 spp. in bromeliads, 21 spp. in heliconia), probably due to functional similarities in plant architecture, with the two most abundant species comprising 60,62% of the total community. Coefficients of similarity were low because of marked differences in species assemblages. 4. Some taxa were phytotelm generalists but most showed a preference for one particular habitat, indicating differential selection in the choice of oviposition sites and larval development within the forest ecosystem. In common with many island communities, species richness was lower than that reported for these phytotelm habitats in mainland central and south America. [source] Linking dispersal, immigration and scale in the neutral theory of biodiversityECOLOGY LETTERS, Issue 12 2009Ryan A. Chisholm Abstract In the classic spatially implicit formulation of Hubbell's neutral theory of biodiversity a local community receives immigrants from a metacommunity operating on a relatively slow timescale, and dispersal into the local community is governed by an immigration parameter m. A current problem with neutral theory is that m lacks a clear biological interpretation. Here, we derive analytical expressions that relate the immigration parameter m to the geometry of the plot defining the local community and the parameters of a dispersal kernel. Our results facilitate more rigorous and extensive tests of the neutral theory: we conduct a test of neutral theory by comparing estimates of m derived from fits to empirical species abundance distributions to those derived from dispersal kernels and find acceptable correspondence; and we generate a new prediction of neutral theory by investigating how the shapes of species abundance distributions change theoretically as the spatial scale of observation changes. We also discuss how our main analytical results can be used to assess the error in the mean-field approximations associated with spatially implicit formulations of neutral theory. Ecology Letters (2009) 12: 1385,1393 [source] Effect of artificial mixtures of environmental polycyclic aromatic hydrocarbons present in coal tar, urban dust, and diesel exhaust particulates on MCF-7 cells in cultureENVIRONMENTAL AND MOLECULAR MUTAGENESIS, Issue 2 2004Brinda Mahadevan Abstract Human exposure to polycyclic aromatic hydrocarbons (PAHs) occurs through complex mixtures. The National Institute of Standards and Technology has established standard reference materials (SRMs) for selected PAH mixtures that are composed of carcinogenic, noncarcinogenic, and weakly carcinogenic compounds, such as those derived from coal tar (SRM 1597), atmospheric particulate matter (SRM 1649), and diesel particulate matter (SRM 1650). To study the effects of PAHs with different carcinogenic potential in complex mixtures, and to investigate the metabolic activation of noncarcinogenic and weakly carcinogenic PAHs to DNA-binding derivatives, artificial mixtures (1597H, 1649H, and 1650H) were prepared in the laboratory. These artificial mixtures contained the same relative ratios of noncarcinogenic and weakly carcinogenic PAHs present in SRM 1597, SRM 1649, and SRM 1650. The human mammary carcinoma-derived cell line MCF-7 was treated with these artificial mixtures and analyzed for PAH-DNA adduct formation and the induction of cytochrome P450 (CYP) enzymes. We found that the artificial mixtures formed lower but detectable levels of DNA adducts 24 and 48 hr after treatment than benzo[a]pyrene. Induction of CYP enzyme activity was measured by the ethoxyresorufin- O -deethylase assay, and the expression of CYP1A1 and CYP1B1 was confirmed by immunoblots. Both noncarcinogenic and weakly carcinogenic PAHs present in the artificial mixtures have the ability to induce CYP1A1 and CYP1B1 in MCF-7 cells and contribute to DNA binding. Therefore, it is necessary to take into account the noncarcinogenic and weakly carcinogenic PAHs present in environmental mixtures in assessing the potential risk associated with human exposure. Environ. Mol. Mutagen. 44:99,107, 2004. © 2004 Wiley-Liss, Inc. [source] On the Way to Glycoprocessing Inhibitors , Synthesis of an Imidazolo-Nectrisine-Phosphono Acid Derivative: A Potential Glycosyltranferase InhibitorEUROPEAN JOURNAL OF ORGANIC CHEMISTRY, Issue 15 2003Théophile Tschamber Abstract Assuming the transition state of glycosyltransferase inhibitors to be similar to those encountered with potent glycosidase inhibitors , i.e. a flattened conformation with a positively charged anomeric centre , we worked out a synthesis of the D - arabino -configured phosphonic acid target molecule 2 derived from an imidazolo-sugar. The key synthetic intermediate is the linear imidazolo L - xylo compound 10 which could be obtained, either from L - threo precursor 6 by a coupling reaction with imidazole derivative 5, or from L -sorbose. A multi-step and site specific iodination of 10 gave the mono-iodo- L - xylo derivative 14 which was cyclised to the D - arabino -configured bicyclic azasugar 15. Phosphorylation of the Grignard derivative of the latter, followed by mono-esterification with citronellol along with some protection-deprotection steps led to target molecule 2. The potential inhibitor 2 is supposed to be protonated at its most basic N atom by a carboxylic acid residue in the arabinosyl-transferase active site. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2003) [source] Val-Ala Dipeptide Isosteres by Hydrocyanation of ,,-Amino ,,,-Unsaturated Ketones , Control of Stereoselectivity by the N -Protecting GroupEUROPEAN JOURNAL OF ORGANIC CHEMISTRY, Issue 10 2003Fabio Benedetti Abstract Three diastereoisomeric hydroxyethylene isosters of the Val-Ala dipeptide were synthesized from ,,,-unsaturated ketones 1 derived from N -Boc- and N,N -dibenzyl- L -valine. The enones were hydrocyanated with diethylaluminum cyanide to give the corresponding ,-cyano ketones with the stereoselectivity depending on the protecting group. N -Boc protected enone 1a gave a 1:1 mixture of anti and syn adducts 4a, 5a while the corresponding N,N -dibenzyl compound 1c gave a 6:1 mixture of anti, syn adducts 4c, 5c. Borohydride reduction of the resulting cyano ketones is also controlled by the protecting group, resulting in opposite stereoselectivities for N -Boc and N,N -dibenzyl compounds. The cyano alcohols thus obtained were converted, in several steps, into two series of enantiomerically pure hydroxyethylene isosters of the Val-Ala dipeptide. In the first series the hydroxy group and the N -terminal of the isoster are internally protected through the formation of an oxazolidine; in the second series the hydroxy group and the C-terminal are protected as lactone. Two oxazolidines (28, 29), corresponding to syn,syn and syn,anti 4-hydroxy-5-amino acid isosters, and three lactones (23,25), corresponding to syn,syn, syn,anti, and anti,anti isosters were obtained by this approach. (© Wiley-VCH Verlag GmbH & Co KGaA, 69451 Weinheim, Germany, 2003) [source] Composition of organic matter in a subtropical Acrisol as influenced by land use, cropping and N fertilization, assessed by CPMAS 13C NMR spectroscopyEUROPEAN JOURNAL OF SOIL SCIENCE, Issue 6 2005J. Dieckow Summary We know much about the influence of management on stocks of organic matter in subtropical soils, yet little about the influence on the chemical composition. We therefore studied by CPMAS 13C NMR spectroscopy the composition of the above-ground plant tissue, of the organic matter of the whole soil and of silt- and clay-size fractions of the topsoil and subsoil of a subtropical Acrisol under grass and arable crops. Soil samples were collected from three no-till cropping systems (bare soil; oats,maize; pigeon pea + maize), each receiving 0 and 180 kg N ha,1 year,1, in a long-term field experiment. Soil under the original native grass was also sampled. The kind of arable crops and grass affected the composition of the particulate organic matter. There were no differences in the composition of the organic matter in silt- and clay-size fractions, or of the whole soil, among the arable systems. Changes were observed between land use: the soil of the grassland had larger alkyl and smaller aromatic C contents than did the arable soil. The small size fractions contain microbial products, and we think that the compositional difference in silt- and clay-size fractions between grassland and the arable land was induced by changes in the soil's microbial community and therefore in the quality of its biochemical products. The application of N did not affect the composition of the above-ground plant tissue nor of the particulate organic matter and silt-size fractions, but it did increase the alkyl C content in the clay-size fraction. In the subsoil, the silt-size fraction of all treatments contained large contents of aromatic C. Microscopic investigation confirmed that this derived from particles of charred material. The composition of organic matter in this soil is affected by land use, but not by variations in the arable crops grown. [source] Platypus Pou5f1 reveals the first steps in the evolution of trophectoderm differentiation and pluripotency in mammalsEVOLUTION AND DEVELOPMENT, Issue 6 2008Hitoshi Niwa SUMMARY Uterine nourishment of embryos by the placenta is a key feature of mammals. Although a variety of placenta types exist, they are all derived from the trophectoderm (TE) cell layer of the developing embryo. Egg-laying mammals (platypus and echidnas) are distinguished by a very short intrauterine embryo development, in which a simple placenta forms from TE-like cells. The Pou5f1 gene encodes a class V POU family transcription factor Oct3/4. In mice, Oct3/4 together with the highly conserved caudal -related homeobox transcription factor Cdx2, determines TE fate in pre-implantation development. In contrast to Cdx2, Pou5f1 has only been identified in eutherian mammals and marsupials, whereas, in other vertebrates, pou2 is considered to be the Pou5f1 ortholog. Here, we show that platypus and opossum genomes contain a Pou5f1 and pou2 homolog, pou2-related, indicating that these two genes are paralogues and arose by gene duplication in early mammalian evolution. In a complementation assay, we found that platypus or human Pou5f1, but not opossum or zebrafish pou2, restores self-renewal in Pou5f1 -null mouse ES cells, showing that platypus possess a fully functional Pou5f1 gene. Interestingly, we discovered that parts of one of the conserved regions (CR4) is missing from the platypus Pou5f1 promoter, suggesting that the autoregulation and reciprocal inhibition between Pou5f1 and Cdx2 evolved after the divergence of monotremes and may be linked to the development of more elaborate placental types in marsupial and eutherian mammals. [source] Growth inhibition of mammalian cells by eosinophil cationic proteinFEBS JOURNAL, Issue 1 2002Takashi Maeda Eosinophil cationic protein (ECP), one of the major components of basic granules of eosinophils, is cytotoxic to tracheal epithelium. However, the extent of this effect on other cell types has not been evaluated in vitro. In this study, we evaluated the effect of ECP on 13 mammalian cell lines. ECP inhibited the growth of several cell lines including those derived from carcinoma and leukemia in a dose-dependent manner. The IC50 values on A431 cells, MDA-MB-453 cells, HL-60 cells and K562 cells were,estimated to be ,,1,5 µm. ECP significantly suppressed the size of colonies of A431 cells, and decreased K562 cells in G1/G0 phase. However, there was little evidence that ECP killed cells in either cell line. These effects of ECP were not enhanced by extending its N-terminus. Rhodamine B isothiocyanate-labeled ECP started to bind to A431 cells after 0.5 h and accumulated for up to 24 h, indicating that specific affinity for the cell surface may be important. The affinity of ECP for heparin was assessed and found to be reduced when tryptophan residues, one of which is located at a position in the catalytic subsite of ribonuclease in ECP, were modified. The growth-inhibitory effect was also attenuated by this modification. These results suggest that growth inhibition by ECP is dependent on cell type and is cytostatic. [source] GC,MS analysis of volatile hydrolysis products from glucosinolates in Farsetia aegyptia var. ovalisFLAVOUR AND FRAGRANCE JOURNAL, Issue 2 2003A. A. Al-Gendy Abstract Twenty-two volatile glucosinolate hydrolysis products from seeds and leaves of Farsetia aegyptia var. ovalis were identi,ed and quanti,ed using both natural autolysis and exogenous myrosinase, followed by GC,MS. The 22 isothiocyanate thiocyanate, epithioalkane nitrile and nitrile hydrolysis products identi,ed can be rationalized, assuming 13 glucosinolates in the seeds and 12 in the leaves. Among the volatile hydrolysis products observed, those derived from allylglucosinolates and 3-methylsulphinylpropylglucosinolate (glucoiberin) were prominent. Copyright © 2003 John Wiley & Sons, Ltd. [source] The microstratigraphic record of abrupt climate changes in cave sediments of the Western MediterraneanGEOARCHAEOLOGY: AN INTERNATIONAL JOURNAL, Issue 5 2001Marie-Agnès Courty The purpose of this paper is to illustrate how calcareous sediments from Pleistocene and Holocene rockshelters and open caves of the Western Mediterranean can provide a stratigraphic record of abrupt climate change. The method proposed here is based on microstratigraphic examination of sedimentary sequences using microscopic techniques. The most important processes for characterizing the sensitivity of each cave to climate variables are: (1) the modes and rate of carbonate sediment production, (2) the nature and intensity of the pedogenic processes responsible for the synchronous alteration of carbonate materials (either those derived from the cave walls or those deposited on the ground surface), and (3) the supply of allogenic sediments, particularly by eolian activity. The cave sediment sequences presented record the marked coolings known as Dansgaard-Oeschger stadials and Heinrich events that occurred during the Pleistocene and the Holocene, as demonstrated by the high resolution records from ice and deep sea cores. At Abric Romanì in northeastern Spain, a series of sharp climatic deteriorations of increasing severity is shown to have occurred synchronously with the transition from the Middle to the Upper Paleolithic, with a period of seasonal frost and strong winds at ca. 37,000 yr B.P., tentatively correlated with Heinrich event 4. At Pigeon Cave, Taforalt (northern Morocco), the transition from the Aterian to Ibero-Maurusian/Epipalaeolithic cultures is dated to around 24,000,20,000 yr B.P. and is punctuated by a series of short cold pulses with evidence for seasonal freezing, soil erosion, and minimal evapotranspiration. In El Miron cave in north-central Spain, the exceptional nature of the Younger Dryas cooling produced a marked destabilization of the cave walls and roof. At El Miron, the stratigraphic evidence for sediment removal due to the rapid percolation of snow melt under a degraded soil cover allows us to reconstruct the nature of the negative excursion at ca. 8200 yr B.P. This example also illustrates how climate-controlled pedogenic processes can create a stratigraphic signature which has often been confused with a sedimentary hiatus. We conclude that cave sediments provide a valuable record of Pleistocene and Holocene climate changes. In appropriate contexts, these sequences allow us to examine the ecological stress generated by these unique global events at a local and regional level and improve our understanding of the complex anthropological processes that occurred at the same time. © 2001 John Wiley & Sons, Inc. [source] Gas breakthrough experiments on fine-grained sedimentary rocksGEOFLUIDS (ELECTRONIC), Issue 1 2002A. Hildenbrand Abstract The capillary sealing efficiency of fine-grained sedimentary rocks has been investigated by gas breakthrough experiments on fully water saturated claystones and siltstones (Boom Clay from Belgium, Opalinus Clay from Switzerland and Tertiary mudstone from offshore Norway) of different lithological compositions. Sand contents of the samples were consistently below 12%, major clay minerals were illite and smectite. Porosities determined by mercury injection lay between 10 and 30% while specific surface areas determined by nitrogen adsorption (BET method) ranged from 20 to 48 m2 g , 1. Total organic carbon contents were below 2%. Prior to the gas breakthrough experiments the absolute (single phase) permeability (kabs) of the samples was determined by steady state flow tests with water or NaCl brine. The kabs values ranged between 3 and 550 nDarcy (3 × 10,21 and 5.5 × 10,19 m2). The maximum effective permeability to the gas-phase (keff) measured after gas breakthrough on initially water-saturated samples extended from 0.01 nDarcy (1 × 10,23 m2) up to 1100 nDarcy (1.1 × 10,18 m2). The residual differential pressures after re-imbibition of the water phase, referred to as the ,minimum capillary displacement pressures' (Pd), ranged from 0.06 to 6.7 MPa. During the re-imbibition process the effective permeability to the gas phase decreases with decreasing differential pressure. The recorded permeability/pressure data were used to derive the pore size distribution (mostly between 8 and 60 nm) and the transport porosity of the conducting pore system (10 -5,10 -2%). Correlations could be established between (i) absolute permeability coefficients and the maximum effective permeability coefficients and (ii) effective or absolute permeability coefficients and capillary sealing efficiency. No correlation was found between the capillary displacement pressures determined from gas breakthrough experiments and those derived theoretically by mercury injection. [source] A thermochemical boundary layer at the base of Earth's outer core and independent estimate of core heat fluxGEOPHYSICAL JOURNAL INTERNATIONAL, Issue 3 2008David Gubbins SUMMARY Recent seismological observations suggest the existence of a ,150-km-thick density-stratified layer with a P -wave velocity gradient that differs slightly from PREM. Such a structure can only be caused by a compositional gradient, effects of a slurry or temperature being too small and probably the wrong sign. We propose a stably stratified, variable concentration layer on the liquidus. Heat is transported by conduction down the liquidus while the light and heavy components migrate through the layer by a process akin to zone refining, similar to the one originally proposed by Braginsky. The layer remains static in a frame of reference moving upwards with the expanding inner core boundary. We determine the gradient using estimates of co, the concentration in the main body of the outer core, and cb, the concentration of the liquid at the inner core boundary. We determine the depression of the melting point and concentrations using ideal solution theory and seismologically determined density jumps at the inner core boundary. We suppose that co determines ,,mod, the jump from normal mode eigenfrequencies that have long resolution lengths straddling the entire layer, and that cb determines ,,bod, the jump determined from body waves, which have fine resolution. A simple calculation then yields the seismic, temperature, and concentration profiles within the layer. Comparison with the distance to the C-cusp of PKP and normal mode eigenfrequencies constrain the model. We explore a wide range of possible input parameters; many fail to predict sensible seismic properties and heat fluxes. A model with ,,mod= 0.8 gm cc,1, ,,bod= 0.6 gm cc,1, and layer thickness 200 km is consistent with the seismic observations and can power the geodynamo with a reasonable inner core heat flux of ,2 TW and nominal inner core age of ,1 Ga. It is quite remarkable and encouraging that a model based on direct seismic observations and simple chemistry can predict heat fluxes that are comparable with those derived from recent core thermal history calculations. The model also provides plausible explanations of the observed seismic layer and accounts for the discrepancy between estimates of the inner core density jumps derived from body waves and normal modes. [source] Regional GPS data confirm high strain accumulation prior to the 2000 June 4 Mw= 7.8 earthquake at southeast SumatraGEOPHYSICAL JOURNAL INTERNATIONAL, Issue 3 2001G. W. Michel Summary Site velocities derived from repeated measurements in a regional GPS network in Southeast Asia help to constrain the motion of tectonic blocks as well as slip rates along major faults in the area. Using 3-D forward dislocation modelling, the influence of seismic elastic loading and unloading on the measured site motions are approximated. Results suggest that the northwestern Sunda arc is fully coupled seismogenically, whereas its eastern part along Java shows localized deformation. Higher horizontal velocity gradients than expected from the modelling of a fully coupled plate interface west of Manila in the Philippines suggest that deformation may be localized there. Assuming that geodetically derived convergence represents long-term rates, accumulated geodetic moments are compared to those derived using seismic data from 1977 to 2000 (Harvard CMT catalogue). If areas displaying localized deformation are dominated by creep processes, the largest difference between accumulated and seismically released deformation is located where the 2000 June 4 Mw = 7.8 Sumatra earthquake occurred. [source] Application of a three-dimensional ray-tracing technique to global P, PP and Pdiff traveltime tomographyGEOPHYSICAL JOURNAL INTERNATIONAL, Issue 3 2001A. Gorbatov Summary A 3-D ray-path tracing algorithm was successfully applied to global P -wave traveltime tomography. The inversion was conducted iteratively using the resultant P -wave velocity model as the initial model for the subsequent iteration. The LSQR method was adopted to solve a large and sparse system of equations. This iteratively linearized inversion with 3-D ray tracing increased wave-speed anomalies, located heterogeneities better and reduced smearing as compared to those derived from a conventional one-step inversion using 1-D ray tracing, although the general pattern of velocity anomalies was similar. A major difference was found in the lowermost mantle, where the departure of a ray path from the great circle path tends to be in general greatest. In particular, a pronounced high-velocity anomaly develops beneath the Indian Ocean, a feature not obvious in the result of 1-D inversion. The final P -wave velocity model was obtained by including reported PP and Pdiff traveltime data. The addition of the PP data sharpened the images and enhanced velocity anomalies in the upper mantle, especially at latitudes above 45° of the Northern Hemisphere. The addition of the Pdiff data sharpened and amplified velocity anomalies in the lowermost mantle in general. [source] Are more productive varieties of Paspalum dilatatum less tolerant to drought?GRASS & FORAGE SCIENCE, Issue 3 2010L. L. Couso Abstract Paspalum dilatatum Poir., is a perennial C4 grass widely distributed in the Argentinean Pampas. The response to water availability for materials developed with forage-production purposes is unknown. We hypothesized that genetic differences between commercial varieties are reflected in their regrowth capacity under water stress. The effect of five levels of constant water supply on three plant varieties (two derived from apomictic materials: ,Relincho' and ,Alonso' and one from sexually-derived material: ,Primo') were examined in the greenhouse. Leaf- and plant-response traits were followed during 38 d after a single defoliation event. Seven response variables were measured: three of them were morphogenetic (leaf elongation rate, leaf appearance rate and leaf elongation duration) and four were structural (number of live leaves, lamina length, tiller biomass and tiller production). The sexual material showed higher values for growth variables than the apomictic varieties (leaf elongation rate, leaf length and tiller biomass) across the environmental range. Apomictic varieties showed a proportionally similar drought response to the sexual material for the seven variables. No intra-specific trade-off (statistical interaction) was found between growth under high water availability conditions and drought tolerance. [source] Differences in Labor versus Value Chain Industry Clusters: An Empirical InvestigationGROWTH AND CHANGE, Issue 3 2007HENRY RENSKI ABSTRACT Regional analysts often identify industry clusters according to a single dimension of industrial interdependence, typically by trading patterns as revealed in national or regionalized input,output data. This is despite the fact that the theory underpinning regional industry cluster applications draws heavily on Marshall's theory of external economies, including the important role of labor pooling economies and knowledge spillovers in addition to spatially co-located suppliers. This article investigates whether industry clusters identified based on trading relationships (value chain clusters) are meaningfully different in industrial composition and geography than those derived from an analysis of occupational employment requirements (labor-based clusters). The results suggest that value chain linkages are a weak proxy for shared labor requirements, and vice versa. [source] Imputation of SF-12 Health Scores for Respondents with Partially Missing DataHEALTH SERVICES RESEARCH, Issue 3 2005Honghu Liu Objective. To create an efficient imputation algorithm for imputing the SF-12 physical component summary (PCS) and mental component summary (MCS) scores when patients have one to eleven SF-12 items missing. Study Setting. Primary data collection was performed between 1996 and 1998. Study Design. Multi-pattern regression was conducted to impute the scores using only available SF-12 items (simple model), and then supplemented by demographics, smoking status and comorbidity (enhanced model) to increase the accuracy. A cut point of missing SF-12 items was determined for using the simple or the enhanced model. The algorithm was validated through simulation. Data Collection. Thirty-thousand-three-hundred and eight patients from 63 physician groups were surveyed for a quality of care study in 1996, which collected the SF-12 and other information. The patients were classified as "chronic" patients if they reported that they had diabetes, heart disease, asthma/chronic obstructive pulmonary disease, or low back pain. A follow-up survey was conducted in 1998. Principal Findings. Thirty-one percent of the patients missed at least one SF-12 item. Means of variance of prediction and standard errors of the mean imputed scores increased with the number of missing SF-12 items. Correlations between the observed and the imputed scores derived from the enhanced models were consistently higher than those derived from the simple model and the increments were significant for patients with ,6 missing SF-12 items (p<.03). Conclusion. Missing SF-12 items are prevalent and lead to reduced analytical power. Regression-based multi-pattern imputation using the available SF-12 items is efficient and can produce good estimates of the scores. The enhancement from the additional patient information can significantly improve the accuracy of the imputed scores for patients with ,6 items missing, leading to estimated scores that are as accurate as that of patients with <6 missing items. [source] Synthesis and Enzymic Hydrolysis of Oligoribonucleotides Incorporating 3-Deazaguanosine: The Importance of the Nitrogen-3 Atom of Single Conserved Guanosine Residues on the Catalytic Activity of the Hammerhead RibozymeHELVETICA CHIMICA ACTA, Issue 8 2003Frank Seela Four base-modified hammerhead ribozyme/substrate complexes were constructed in which single guanosine (1) residues were replaced by 3-deazaguanosine (2) in the positions G5, G8, GL2.1, and G12. The base-modified ribozyme complexes were prepared by solid-phase synthesis of oligoribonucleotides employing the novel phosphoramidite 3 derived from 2. Phosphoramidite 3 carried a phenoxyacetyl group at the amino function and a diphenylcarbamoyl residue at the oxo group of the nucleobase. The 2,-hydroxy group was blocked with a triisopropylsilyl residue. Kinetic analysis of the phosphodiester hydrolysis showed a moderate decrease of the ribozyme catalytic activity when the residues G5 or G8 were replaced by 3-deazaguanosine and a 200-fold decrease when G12 was substituted. A 6-fold catalytic increase occurred when 3-deazaguanosine was replacing GL2.1 in the loop region. The data indicate that the N(3) atom of compound 2, in particular at position G12 is critical for the ribozyme activity. [source] Th1 cytokine,induced downregulation of PPAR, in human biliary cells relates to cholangitis in primary biliary cirrhosis,HEPATOLOGY, Issue 6 2005Kenichi Harada Peroxisome proliferator-activated receptor-, (PPAR,) is known to inhibit the production of proinflammatory cytokines. In Th1-predominant diseases, PPAR, ligands can ameliorate clinical severity by downregulating the expression of proinflammatory cytokines. Primary biliary cirrhosis (PBC) is characterized by chronic destructive cholangitis with a Th1-predominant cytokine milieu. Unusual immune responses to infectious agents are suspected to underlie its etiopathogenesis. We examined the significance of PPAR, in biliary inflammation in connection to PBC. To this end, we performed immunohistochemistry, quantitative polymerase chain reaction, and nuclear factor-kappaB (NF-,B) DNA-binding assays to clarify the intrahepatic distribution of PPAR, and the regulation of PPAR, by inflammatory cytokines and PPAR, ligand in five cultured biliary cell lines including one derived from PBC liver. In liver specimens from patients with PBC, PPAR, protein was ubiquitously expressed in intrahepatic biliary epithelium, whereas the expression of PPAR, protein and mRNA was reduced in damaged bile ducts. PPAR, expression in cultured cells was upregulated by interleukin-4 (IL-4; Th2-type), but downregulated by IFN-, (Th1-type). PPAR, ligand negatively modulated lipopolysaccharide-induced NF-,B activation. Moreover, this inhibitory effect of PPAR, ligand was attenuated by pretreatment with IFN-,. In conclusion, PPAR, may be important to maintain homeostasis in the intrahepatic biliary epithelium, and its reduction in the bile ducts of PBC liver may be associated with the Th1-predominant milieu and with the development of chronic cholangitis in PBC. Immunosuppression using PPAR, ligands may be of therapeutic benefit to attenuate biliary inflammation in PBC. (HEPATOLOGY 2005;41.) [source] Topographic parameterization in continental hydrology: a study in scaleHYDROLOGICAL PROCESSES, Issue 18 2003Robert N. Armstrong Abstract Digital elevation models (DEMs) are useful and popular tools from which topographic parameters can be quickly and efficiently extracted for various hydrologic applications. DEMs coupled with automated methods for extracting topographic information provide a powerful means of parameterizing hydrologic models over a wide range of scales. However, choosing appropriate DEM scales for particular hydrologic modelling applications is limited by a lack of understanding of the effects of scale and grid resolution on land-surface representation. The scale effects of aggregation on square-grid DEMs of two continental-scale basins are examined. Base DEMs of the Mackenzie and Missouri River basins are extracted from the HYDRO1k DEM of North America. Successively coarser grids of 2, 4, 8, , 64 km were generated from the ,base' DEMs using simple linear averaging. TOPAZ (Topographic Parameterization) was applied to the base and aggregated DEMs using constant critical source area and minimum source channel length values to extract topographic variables at varying scales or resolutions. The effects of changing DEM resolution are examined by considering changes in the spatial distribution and statistical properties of selected topographic variables of hydrological importance. The effects of increasing grid size on basin and drainage network delineation, and derived topographic variables, tends to be non-linear. In particular, changes in overall basin extent and drainage network configuration make it impractical to apply a simple scaling function to estimate variable values for fine-resolution DEMs from those derived from coarse-resolution DEMs. Results also suggest the resolution to which a DEM can be reduced by aggregation and still provide useful topographic information for continental-scale hydrologic modelling is that at which the mean hydraulic slope falls to approximately 1%. In this study, that generally occurred at a resolution of about 10 km. Copyright © 2003 John Wiley & Sons, Ltd. [source] Simulation of ice phenology on Great Slave Lake, Northwest Territories, CanadaHYDROLOGICAL PROCESSES, Issue 18 2002Patrick Ménard Abstract A one-dimensional thermodynamic lake ice model (Canadian Lake Ice Model or CLIMo) is used to simulate ice phenology on Great Slave Lake (GSL) in the Mackenzie River basin, Northwest Territories, Canada. Model simulations are validated against freeze-up and break-up dates, as well as ice thickness and on-ice snow depth measurements made in situ at three sites on GSL (Back Bay near Yellowknife, 1960,91; Hay River, 1965,91; Charlton Bay near Fort Reliance, 1977,90). Freeze-up and break-up dates from the lake ice model are also compared with those derived from SSM/I 85 GHz passive microwave imagery over the entire lake surface (1988,99). Results show a very good agreement between observed and simulated ice thickness and freeze-up/break-up dates over the 30,40 years of observations, particularly for the Back Bay and Hay River sites. CLIMo simulates the ice thickness and annual freeze-up/break-dates with a mean error of 7 cm and 4 days respectively. However, some limitations have been identified regarding the rather simplistic approach used to characterize the temporal evolution of snow cover on ice. Future model improvements will therefore focus on this particular aspect, through linkage or coupling to a snow model. Copyright © 2002 John Wiley & Sons, Ltd. [source] A simple model of hillslope response for overland flow generationHYDROLOGICAL PROCESSES, Issue 17 2001Professor Carmelo Agnese Abstract This paper deals with the derivation of the hydrological response of a hillslope on the assumption of quick runoff by surface runoff generation. By using the simple non-linear storage based model, first proposed by Horton, an analytical solution of the overland flow equations over a plane hillslope was derived. This solution establishes a generalization for different flow regimes of Horton's original solution, which is valid for the transitional flow regime only. The solution proposed was compared successfully with that of Horton and, for the turbulent flow regime, to the one derived from kinematic wave theory. This solution can be applied easily to both stationary and non-stationary rainfall excess events. An analytical solution for the instantaneous response function (IRF) was also derived. Finally, simple expressions to compute peak and time to peak of IRF are proposed. Copyright © 2001 John Wiley & Sons, Ltd. [source] EGFR tyrosine kinase inhibition radiosensitizes and induces apoptosis in malignant glioma and childhood ependymoma xenograftsINTERNATIONAL JOURNAL OF CANCER, Issue 1 2008Birgit Geoerger Abstract Malignant gliomas and childhood ependymomas have a high rate of treatment failure. Epidermal growth factor receptor (EGFR) activation has been implicated in the tumorigenesis and radioresistance of many cancers, including brain tumors. Therefore, combining EGFR targeting with irradiation is a potentially attractive therapeutic option. We evaluated the tyrosine kinase inhibitor gefitinib for its antitumor activity and potential to radio-sensitize in vivo in two xenograft models: an EGFR amplified glioma and an EGFR expressing ependymoma, both derived from primary tumors. When administered at 100 mg/kg for 5 consecutive days, gefitinib-induced partial tumor regression in all treated EGFR amplified IGRG88 glioma xenografts. The addition of 1 Gy of irradiation prior to gefitinib administration resulted in 5 complete and 4 partial regressions for the 9 treated tumors as well as a significant tumor growth delay of 33 days for the combined treatment compared to 19 days for each therapy alone, suggesting additive antitumor activity. Tumor regression was associated with inhibition of AKT and MAPK pathways by gefitinib. In contrast, the ependymoma IGREP83 was sensitive to irradiation, but remained resistant to gefitinib. Combined treatment was associated with inhibition of radiation-induced MAPK phosphorylation and significant induction of apoptotic cell death though radiation-induced AKT phosphorylation was maintained. Depending on the scheduling of both therapies, a trend towards superior antitumor activity was observed with combined treatment. Thus, EGFR targeting through tyrosine kinase inhibition appears to be a promising new approach in the treatment of EGFR-driven glioma, particularly in combination with radiation therapy. © 2008 Wiley-Liss, Inc. [source] | ||||||||||||||||||||||||||||||||||