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Selected AbstractsIdentification of Acipenseriformes species in tradeJOURNAL OF APPLIED ICHTHYOLOGY, Issue 2008A. Ludwig Summary Sturgeons and paddlefishes (Acipenseridae) are highly endangered freshwater fishes. Their eggs (sold as caviar) are one of the most valuable wildlife products in international trade. Concerns of overharvesting and the conservation status of many of the 27 extant species of Acipenseriformes led to all species being included on the CITES Appendices in 1998. Since then international trade in all products and parts from sturgeon and paddlefish has been regulated. However, despite the controls on trade, unsustainable harvesting continues to threaten many populations. Illegal fishing and trade continues to be a threat to the management of these fish. To enforce the regulation of legal trade and prevention of illegal trade, the development of a uniform identification system for parts and derivates of Acipenseriformes has been identified as an urgent requirement. Ideally this system should be suitable for (i) identification at the species-level of caviar and other products from Acipenseriformes; (ii) population identification; (iii) source identification (wild vs aquaculture); and (iv) determining the age of caviar because strict timeframes govern its international trade. This paper reviews the techniques currently available and their potential to be used in an identification system for Acipenseriformes species and their products in trade. A review of all available identification techniques has shown that there is not a single method that can meet all requirements (see i,iv), and it does not appear to be feasible to develop such a method in the near future therefore the most appropriate methods need to be developed for each. Considering the advantages and disadvantages of all techniques reviewed in this document, the following conclusions can be drawn: (i) for the identification of species, approaches are recommended that target mitochondrial cytochrome b sequences (RFLP, nested PCR or direct sequencing). However, they show limitations for the detection of hybrids (although natural hybrids are rare, the number of artificially produced hybrids in aquaculture is increasing) and for the differentiation of the following closely related species complexes: Acipenser gueldenstaedti,Acipenser baerii,Acipenser persicus,Acipenser naccarii; Acipenser medirostris,Acipenser mikadoi; and Scaphirhynchus albus,Scaphirhynchus plathorhynchus,Scaphirhynchus suttkusi; (ii) the identification of different populations of the same species is currently not feasible because genetic data are incomplete for most populations, and stocking and release programmes, which have become more and more common, often result in a mixture of phenotypes and genotypes, thereby impeding the creation and application of such a population identification system; (iii) source identification based on genetic approaches can be excluded at present because there are no genetic differences between wild and hatchery-raised fish. This is the result of the continuing restocking of natural populations with captive fish and vice versa. However, because rearing (i.e. environmental) conditions are different, methods focusing on differences in water quality or food seem to be more appropriate (for example differences in fatty acid composition). So far, very few studies have been conducted and therefore, source identification methods merit further exploration; and (iv) the age of a product in trade cannot be detected by DNA-based methods and protein profiling is undoubtedly impractical due to hard-to-perform, labour-and cost-intensive methods, which are highly susceptible to protein degradation. Arising from the limits discussed above, the next steps in the development of a uniform sturgeon identification system are proposed to be the following: (i) designation of qualified reference laboratories at national levels in (re-) exporting and importing countries. These should be approved through a standardized testing procedure, for instance a ring test on blind samples. Registered laboratories should be published and disseminated and their accreditations should be subject to certain guarantees regarding quality, economic independence and scientific rigour. Operational procedures have to be determined and standardized among reference laboratories; (ii) establishment of reference collections that are accessible to the reference laboratories containing DNA analyses results and information on the location and availability of tissue samples. This is highly recommended as an important step towards a population identification system and indispensable for a general species identification system; (iii) creation of a website access to the reference collections containing the reference database information about genetic samples, comparable to NCBI, which provides background data: sample location; population information; citation; available genetic data; location of archival storage; currently treated and distributed caviar and status of analysis. This website should also be a forum for the exchange of knowledge on and experiences with identification systems, species and population status information, relevant scientific research, etc.; and (iv) the outcome of the trade identification tests should be made available to the reference laboratories for future reference. The universal caviar labelling system could incorporate an indication of the verification of the consignment. In view of the lack of knowledge and the great need to develop a uniform identification system for Acipenseriformes with regard to the importance of the international caviar trade, further scientific guidance and appropriate research is strongly recommended. Progress should be assessed and exchanged on a regular basis. [source] Genomics and systems biology , how relevant are the developments to veterinary pharmacology, toxicology and therapeutics?JOURNAL OF VETERINARY PHARMACOLOGY & THERAPEUTICS, Issue 3 2005R. F. WITKAMP This review discusses some of the recent developments in genomics and its current and future relevance for veterinary pharmacology and toxicology. With the rapid progress made in this field several new approaches in pharmacological and toxicological research have developed and drug discovery and drug development strategies have changed dramatically. In this review, the term genomics is used to encompass the three sub-disciplines transcriptomics, proteomics and metabolomics (or metabonomics) to describe the formation and fate of mRNA, proteins and metabolites, respectively. The current status and methods of the technology and some applications are briefly described. Although the DNA sequencing programmes are receiving considerable attention, the real value of genomics for pharmacology and toxicology is brought by the parallel developments in bio-informatics, bio-statistics and the integration of biology with mathematics and information technology. The ultimate level of integration is now mostly called systems biology, where mRNA, proteins and metabolites are being analysed in parallel, using a complete arsenal of analytical techniques (DNA-array, LC-MS/MS, GC-MS/MS, NMR, etc.). The information thus collected is analysed, integrated, linked to database information and translated to pathways and systems. This approach offers an enormous potential to study disease mechanisms and find new drug targets. Thus far, genomics and systems biology have not been introduced significantly in typical veterinary pharmacological and toxicological research programmes. The high costs and complexity connected to these large projects often form major obstacles for research groups with limited budgets. In other veterinary areas and disciplines, including infectious diseases, animal production and food-safety more examples of application are available. Genomics and bio-informatics provide outstanding opportunities to study pharmacology and toxicology in a more holistic way, taking into account the complexity of biological systems and based on the basic principles of physiology and the concept of homeostasis. Knowledge of biology, in vivo and in vitro models, and comparative pharmacology/toxicology is essential here, creating excellent opportunities for the veterinary trained scientist. [source] phorest: a web-based tool for comparative analyses of expressed sequence tag dataMOLECULAR ECOLOGY RESOURCES, Issue 2 2004Dag Ahren Abstract Comparative analysis of expressed sequence tags is becoming an important tool in molecular ecology for comparing gene expression in organisms grown in certain environments. Additionally, expressed sequence tag database information can be used for the construction of DNA microarrays and for the detection of single nucleotide polymorphisms. For such applications, we present phorest, a web-based tool for managing, analysing and comparing various collections of expressed sequence tags. It is written in PHP (PHP: Hypertext Preprocessor) and runs on UNIX, Microsoft Windows and Macintosh (Mac OS X) platforms. [source] The law and incomplete database information as confounders in epidemiologic research on occupational injuries and illnesses,AMERICAN JOURNAL OF INDUSTRIAL MEDICINE, Issue 1 2010Arthur Oleinick MD Abstract Background Capture,recapture studies report undercounting of work injuries/illnesses with days away from work (DAFW) in the Bureau of Labor Statistics annual Survey of Occupational Injuries and Illnesses (BLS SOII) by 25,68% depending on the state and undercounting by various state workers' compensation (WC) systems of eligible claims by 5,35%. Methods Statutory/regulatory criteria defining eligible cases are used to adjust counts in the 1998,2001 Minnesota's WC system and the BLS SOII to permit comparison and to evaluate the recent studies. Missing information in the employer database used in the capture,recapture studies is tabulated. An attempt is made to harmonize results with two additional databases counting work injuries. Results Counts in the BLS SOII moderately undercount by 10,16% the number of WC cases. We believe that matching in capture,recapture studies is adversely affected by misperceptions regarding the application of statutory/regulatory eligibility criteria and by missing data. The result is that the reported undercounts in both the BLS SOII and several state WC databases are overstated in the capture,recapture studies. Although three of four databases can be approximately harmonized, the fourth cannot. Conclusions More precisely targeted information is needed before decisions regarding redesign of the BLS survey are made or before legislative or administrative changes in the WC are contemplated. Am. J. Ind. Med. 53:23,36, 2010. © 2009 Wiley-Liss, Inc. [source] | ||||||||||