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Cytological Alterations (cytological + alteration)

Distribution by Scientific Domains

Life Sciences	75%

Selected Abstracts

Cytological Alterations Produced by Sweet Potato Mild Speckling Virus

JOURNAL OF PHYTOPATHOLOGY, Issue 7-8 2006
C. F. Nome
Abstract The potyvirus sweet potato mild speckling (SPMSV) has the biological properties and the coat protein sequence already described. In this work, cytological alterations and the intracellular localization in Ipomoea setosa and Ipomoea batatas was studied. The observations were carried out by means of transmission electron microscopy, complemented with immunogold techniques for the viral localization with SPMSV antiserum of local production. The observations carried out showed almost no alteration on cell components but the presence of cylindrical inclusion in the cytoplasm (bundles, laminate aggregates, and pinwheels, neither circles nor scrolls) belonging to the type-2 in the classification of Edwardson and Christie (Cylindrical Inclusions. Bulletin 894, 1996, pp. 1,11). Gold particles were localized in cytoplasms of all tissues of the leaf. [source]

Thyroid fine needle aspiration: the morphological features on ThinPrep® slide preparations.

CYTOPATHOLOGY, Issue 6 2003
Eighty cases with histological control
This study had several purposes: to define cytomorphological features of thyroid cells that might be modified by alcohol fixation; to optimize May-Grünwald,Giemsa (MGG) staining on ThinPrep® (TP; Cytyc Inc., Bexborough, MA, USA) slides and to compare the diagnostic accuracy of slides prepared by a liquid-based method with those obtained by conventional technique. This study included 120 cases of ultrasound-guided fine needle aspiration (FNA) of the thyroid and 55 FNAs performed on surgically resected thyroid specimens. Histological control was available in 80 cases. In the first group of 120 FNAs, a split-sample technique was used for the TP. Three screenings were performed: first, an individual screening of the conventional smears (CS) and of the TP, a second screening to compare cells observed on the TP with the histological control and a third screening to assess the previously defined diagnostic criteria. Twenty-seven TP cases (22%) were considered unsatisfactory for diagnosis compared with 10 in CS (8%). The high rate of unsatisfactory cases with TP is likely to be due to the use of the split-sample technique. The sensitivity was 94% for CS and 81% for TP. The specificity was 67% and 60% for CS and TP, respectively. Two occult papillary carcinomas were missed by both methods. As for the MGG staining, the modified technique used for TP resulted in the same quality as the standard procedure. Conversely, TP did however induce uncommon morphological features. In this study, sensitivity and specificity levels are higher for CS than for TP; the difference may be explained by the fact that the methanol fixative used for TP induces some cytological alterations, especially in oncocytic tumours and lymphocytic thyroïditis. [source]

Cytomorphological alterations of the thymus, spleen, head-kidney, and liver in cardinal fish (Apogonidae, Teleostei) as bioindicators of stress

JOURNAL OF MORPHOLOGY, Issue 1 2006
Lev Fishelson
Abstract Morphological and cytological alterations at the light microscope (LM) and transmission electron microscope (TEM) levels were observed in the thymus, spleen, head-kidney, and liver of cardinal fishes (Apogonidae, Teleostei) from the Gulf of Aqaba, Red Sea, sampled from a strongly polluted site at the northern end of the gulf, and compared to similar samples from a clean, reference site. At the polluted site, the most prominent change was the formation of numerous deposits of cells rich in phagosomes with lipofucin, melanin granules, and phagocytosed debris, including a high increase in number and dimensions of Hassall's corpuscles and melano-macrophage centers. The number of Hassall's corpuscles was 20 (±8.0)/mm2 and of melano-macrophage centers 18 (±4.0)/mm2 at the polluted site, and 7.0 (±4.0)/m2 vs. 5.0 (±2.0)/mm2 respectively at the reference site. In numerous instances the head kidney's melano-macrophage centers in fishes from the polluted site were encapsulated by reticulocytes, a phenomenon recognized as a marker of neoplasmosis and possible malignancy. In the spleens of fishes from the polluted site, numerous deposits of cell debris, peroxisomes, and enlarged lysosomes were also observed. The livers (hepatopancreas) of fishes from polluted waters demonstrated very strong hyperlipogeny. Many of their hepatocytes were laden with lipid vesicles, fragmented endoplasmic reticulula, and aberrant mitochondria. Although the observed alterations in the glands and liver do not indicate any immediate threat to the life of the fish, they can become crucial with respect to energy turnover and fecundity trajectories. This study strongly suggests the use of cytological alterations in vital organs, such as were observed, as pathological biomarkers to environmental stress. J. Morphol. © 2005 Wiley-Liss, Inc. [source]