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Cultivation System (cultivation + system)
Selected AbstractsInfluence of Cultivation System on Bioactive Molecules Synthesis in Strawberries: Spin-off on Antioxidant and Antiproliferative ActivityJOURNAL OF FOOD SCIENCE, Issue 1 2010L. D'Evoli ABSTRACT:, Strawberries (Fragaria ananassa,L., cv. favette) were studied to investigate the influence of cultivation practices (biodynamic, conventional) on the synthesis of bioactive molecules (ascorbic acid, ellagic acid, anthocyanins, flavonols) and to evaluate their antioxidant activity. Additionally, the,in vitro,bioactivity, in terms of antioxidant and antiproliferative activity, of the same strawberry samples in human colon carcinoma (Caco-2) cells was also studied. Compared to conventional strawberries, biodynamic fruits had a significantly higher content of ascorbic acid (P,< 0.01), pelargonidin-3-glucoside (P,< 0.05), cyanidin-3-glucoside (P,< 0.01), ellagic acid (P,< 0.01), quercetin, and kaempferol (both,P,< 0.01). Antioxidant activity of biodynamic strawberry crude extract was significantly higher than that of the conventional one (P,< 0.05); in addition, while the antioxidant activity of water-soluble fraction was very similar in both biodynamic and conventional strawberries, that of water-insoluble fraction of biodynamic fruits was significantly higher (P,< 0.05). The same crude extract of biodynamic strawberry samples effectively corresponded to an increase of bioactivity, in terms of both cellular antioxidant activity and antiproliferative activity, in Caco-2 cells differentiated to normal intestinal epithelia and in undifferentiated Caco-2, respectively. Further studies are needed to confirm whether the practice of biodynamic agriculture is likely to increase the bioactivity of other varieties of fruits and vegetables. [source] Cultivation of methanotrophic bacteria in opposing gradients of methane and oxygenFEMS MICROBIOLOGY ECOLOGY, Issue 3 2006Ingeborg Bussmann Abstract In sediments, methane-oxidizing bacteria live in opposing gradients of methane and oxygen. In such a gradient system, the fluxes of methane and oxygen are controlled by diffusion and consumption rates, and the rate-limiting substrate is maintained at a minimum concentration at the layer of consumption. Opposing gradients of methane and oxygen were mimicked in a specific cultivation set-up in which growth of methanotrophic bacteria occurred as a sharp band at either c. 5 or 20 mm below the air-exposed end. Two new strains of methanotrophic bacteria were isolated with this system. One isolate, strain LC 1, belonged to the Methylomonas genus (type I methantroph) and contained soluble methane mono-oxygenase. Another isolate, strain LC 2, was related to the Methylobacter group (type I methantroph), as determined by 16S rRNA gene and pmoA sequence similarities. However, the partial pmoA sequence was only 86% related to cultured Methylobacter species. This strain accumulated significant amounts of formaldehyde in conventional cultivation with methane and oxygen, which may explain why it is preferentially enriched in a gradient cultivation system. [source] Dynamics of yeast prion aggregates in single living cellsGENES TO CELLS, Issue 9 2006Shigeko Kawai-Noma Prions are propagating proteins that are ordered protein aggregates, in which the phenotypic trait is retained in the altered protein conformers. To understand the dynamics of the prion aggregates in living cells, we directly monitored the fate of the aggregates using an on-chip single-cell cultivation system as well as fluorescence correlation spectroscopy (FCS). Single-cell imaging revealed that the visible foci of yeast prion Sup35 fused with GFP are dispersed throughout the cytoplasm during cell growth, but retain the prion phenotype. FCS showed that [PSI+] cells, irrespective of the presence of foci, contain diffuse oligomers, which are transmitted to their daughter cells. Single-cell observations of the oligomer-based transmission provide a link between previous in vivo and in vitro analyses of the prion and shed light on the relationship between the protein conformation and the phenotype. [source] Paddy Rice and the Water SupplyGERMAN RESEARCH, Issue 1 2005Burkhard Sattelmacher Prof. Dr. Over half of Asia,s fresh water is used for growing rice. A new procedure in China has been developed to test a new water-saving cultivation system [source] Application of the WEPP model for prioritization and evaluation of best management practices in an Indian watershedHYDROLOGICAL PROCESSES, Issue 21 2009A. Pandey Abstract The pre-calibrated and validated physically based watershed model, water erosion prediction project (WEPP) was used as a modelling tool for the identification of critical watersheds and evaluation of best management practices for a small hilly watershed (Karso) of India. The land use/cover of the study area was generated using IRS-1C LISS-III (linear imaging self scanner) satellite data. The watershed and sub-watershed boundaries, drainage, slope and soil map of the study area were generated using ARC/INFO geographic information system (GIS). The WEPP model was finally applied to the Karso watershed which lies within Damodar Barakar catchment of India to identify the critical sub-watersheds on the basis of their simulated average annual sediment yields. Priorities were fixed on the basis of ranks assigned to each critical sub-watershed based on the susceptibility to erosion. The sub-watershed having the highest sediment yield was assigned a priority number 1, the next highest value was assigned a priority number 2, and so on. Subsequently, the model was used for evaluating the effectiveness of best management practices (crop and tillage) for conservation of soil for all the sub-watersheds. On the basis of this study, it is realized that cash crops like soyabean should be encouraged in the upland portion of the sub-watersheds, and the existing tillage practice (country plough/mould board plough) may be replaced by a field cultivation system for conservation of soil and water in the sub-watersheds. Copyright © 2009 John Wiley & Sons, Ltd. [source] DEFINITION OF INTERNAL MORPHOLOGY AND STRUCTURAL CHANGES DUE TO DEHYDRATION OF RADISH (RAPHANUS SATIVUS L. CV. SUPRELLA) USING MAGNETIC RESONANCE IMAGING SPECTROSCOPYJOURNAL OF FOOD QUALITY, Issue 5-6 2005ANNA SALERNO ABSTRACT Magnetic resonance imaging (MRI) spectroscopy is a promising nondestructive analytical technique in food science. It offers the unique opportunity of studying vegetables, fruits and other foods in general, in their wholeness without any preparative manipulation of the sample. The aim of this study was to investigate the internal structure of radish and to monitor the variations induced by postharvest storage at low relative humidity. The MRI allowed for a clear definition of the internal structure of radishes with distinct visibility of xylematic and phloematic vessels distributed in a radial way. A decrease in water content, which results in the breakdown of tissues and the formation of large cavities with the detachment of the external cortex, is the main consequence of a few days' storage in low relative humidity. Both of these are factors that drastically decrease the quality of the radish's fleshy root. The MRI images give a novel insight into the internal organization of the hypocotyl, and this offers opportunities for further studies with regard to the structural differences related to the cultivars as well as the cultivation system. [source] Evaluating the enemies hypothesis in a clover-cabbage intercrop: effects of generalist and specialist natural enemies on the turnip root fly (Delia floralis)AGRICULTURAL AND FOREST ENTOMOLOGY, Issue 2 2010Maria Björkman 1The relative importance of the resource concentration hypothesis and the enemies hypothesis was investigated for the turnip root fly Delia floralis in a cabbage,red clover intercropping system compared with a cabbage monoculture. 2Delia floralis egg densities were measured as well as the activity-densities of generalist predators in a field experiment during two growing seasons. In the second year, a study of egg predation with artificially placed eggs was conducted, in addition to a predator exclusion experiment, to estimate total predation during the season. Parasitization rates were estimated from samples of pupae. 3Delia floralis oviposition was greater in the monoculture during both years. The predator activity-densities differed between treatments and study years. The known natural enemies of Delia spp., Bembidion spp. and Aleochara bipustulata showed a strong response to a cultivation system with higher activity-densities in the monoculture. The response, however, appeared to be caused primarily by habitat preferences and not by D. floralis egg densities. 4The reduction in the number of D. floralis pupae in the intercropping may be explained by a disruption in oviposition behaviour caused by the presence of clover because neither predation, nor parasitization rates differed between cultivation systems. [source] Fallow cultivation system and farmers' resource management in Niger, West AfricaLAND DEGRADATION AND DEVELOPMENT, Issue 3 2002A. Wezel Abstract A survey was carried out in 136 farm-households from seven villages in 1995 and 1996 to analyse the traditional fallow cultivation system in Niger. Farmers were asked to give information about land use on their fields, focusing on cropping and fallow periods as well as on cultivation changes compared to the past. In addition, they were interviewed about their management strategies to maintain or improve soil fertility. Millet-based systems clearly dominate at all sites, either in pure form or intercropped with cowpea, groundnut, sorghum or roselle. At present, almost half of all farmers cultivate their fields on average up to 5 years until it is left fallow. About one-third use their fields permanently. Most farmers use short fallow periods of 1 to 5 years. Moreover, there was a decrease in the cropping area left fallow, and the fallow period also decreased steadily in the past years. In the mid-1970s the average fallow period was about 8 years, decreasing to 2.5 years in 1996. The actual fallow periods are too short to allow sufficient positive effects on soil fertility and farmers are aware of this problem. Consequently, farmers employ different fertilization techniques which aim at maintaining or restoring the soil nutrient pool of the fields while providing physical protection against wind and water erosion. Most farmers use animal manure to improve soil fertility and apply mulch from different sources, millet stalks and branches, for soil regeneration. Few farmers employ other strategies such as mineral fertilizer or planting pits. The farmers try to optimize the use of internal and external resources resulting in a mixture of different fertilization and soil protection methods. Internal resources play by far the most important role. Due to the generally limited resource availability farmers concentrate their management efforts on certain areas within each field or on selected fields only. This means a decreased crop production for the individual household and a higher risk of soil degradation because of soil mining or increased erosion risk on the field area where soil fertility management cannot be practised. Copyright © 2002 John Wiley & Sons, Ltd. [source] A convenient and versatile hydroponic cultivation system for Arabidopsis thalianaPHYSIOLOGIA PLANTARUM, Issue 3 2004Hanna Norén A versatile two-step cultivation procedure for Arabidopsis thaliana is described for the production of large quantities of leaf material suitable for biochemical and biophysical analysis. The first step comprises a miniature greenhouse made out of a plastic pipette box to grow the seedlings to the six-leaf stage. For continued growth, the seedlings are transferred to hydroponic cultivation using an opaque container covered by a styrofoam lid. Transfer of the small seedlings to hydroponic culture is facilitated by growth in separate pipette tips, which protects vulnerable roots from damage. The hydroponic cultivation system is easy to scale-up and produces large amounts of relatively large leaves and roots. This hydroponic system produces enough plant material to make Arabidopsis a feasible model for biochemical and biophysical experiments, which can be combined with the available genetic information to address various aspects of plant functional genomics. [source] Thermal tolerance and compatibility zones as a tool to establish the optimum culture condition of the halibut Paralichthys californicus (Ayres, 1859)AQUACULTURE RESEARCH, Issue 7 2010José L. Esquer Mendez Abstract California halibut, Paralichthys californicus (Ayres, 1859) juveniles were studied to ascertain the thermal tolerance and the compatibility zone where these species can be cultivated. Juvenile halibut acclimated at 15, 18, 21 and 24 °C preferred temperatures of 15.1, 18.2, 18.5 and 24.7 °C respectively. The final preferendum (FP) was 18.4 °C, equivalent to the temperature where the physiological processes are more efficient and the optimum growth temperature of 18.02 °C was calculated using the Jobling (1981) equation. The maximum average weekly temperature that must not be exceeded in a juvenile cultivation system is 22.6 °C. Juveniles avoided temperatures of 10.8 and 29.1 °C if they were acclimated between 15 and 24 °C. The thermal tolerance range of the juvenile halibut, having low and high lethal temperatures of 5.0 and 31 °C, characterizes it as a eurythermic organism. The tolerance of the halibut did not increase with the acclimation temperature corresponding to the ultimate upper incipient lethal temperature of 31 °C that differed by only 0.83 °C to the value calculated using the Jobling (1981) equation. The thermal tolerance and compatibility zone for the California halibut were 242.8 and 121.5 (°C)2, respectively; they characterize the thermal niche that includes the FP supporting an optimal growth of juveniles. [source] Microfluidic biolector,microfluidic bioprocess control in microtiter platesBIOTECHNOLOGY & BIOENGINEERING, Issue 3 2010Matthias Funke Abstract In industrial-scale biotechnological processes, the active control of the pH-value combined with the controlled feeding of substrate solutions (fed-batch) is the standard strategy to cultivate both prokaryotic and eukaryotic cells. On the contrary, for small-scale cultivations, much simpler batch experiments with no process control are performed. This lack of process control often hinders researchers to scale-up and scale-down fermentation experiments, because the microbial metabolism and thereby the growth and production kinetics drastically changes depending on the cultivation strategy applied. While small-scale batches are typically performed highly parallel and in high throughput, large-scale cultivations demand sophisticated equipment for process control which is in most cases costly and difficult to handle. Currently, there is no technical system on the market that realizes simple process control in high throughput. The novel concept of a microfermentation system described in this work combines a fiber-optic online-monitoring device for microtiter plates (MTPs),the BioLector technology,together with microfluidic control of cultivation processes in volumes below 1,mL. In the microfluidic chip, a micropump is integrated to realize distinct substrate flow rates during fed-batch cultivation in microscale. Hence, a cultivation system with several distinct advantages could be established: (1) high information output on a microscale; (2) many experiments can be performed in parallel and be automated using MTPs; (3) this system is user-friendly and can easily be transferred to a disposable single-use system. This article elucidates this new concept and illustrates applications in fermentations of Escherichia coli under pH-controlled and fed-batch conditions in shaken MTPs. Biotechnol. Bioeng. 2010;107: 497,505. © 2010 Wiley Periodicals, Inc. [source] Development of a Novel Membrane Aerated Hollow-Fiber MicrobioreactorBIOTECHNOLOGY PROGRESS, Issue 2 2008Louis Villain A new challenge in biotechnological processes is the development of flexible bioprocessing platforms, allowing strain selection, facilitating scale-up and integrating separation steps. Miniaturization of such a cultivation system allows parallel use and the saving of resources but makes the supply of oxygen to the cells difficult. In this work we present a membrane aerated hollow-fiber microbioreactor (HFMBR) which consists of an acrylic glass module equipped with two different types of membrane fibers. Fibers of polyethersulfone and polyvinyldifluoride were used for substrate and oxygen supply, respectively. Cultivation of E. coli as model organism and production of His-tagged GFP were carried out in the extracapillary space of the membrane aerated HFMBR and compared with cultivations in shaking flask which are commonly used for screening experiments. The measurement of the oxygen transfer capacity and the online monitoring of the dissolved oxygen during the cultivation were performed using a fiber optic oxygen sensor. Online measurement of the optical density was also integrated to the bioreactor. Due to efficient oxygen transfer, a better cell growth than in the shaking flask experiments was achieved, while no negative influence on the GFP productivity was observed in the membrane aerated bioreactor. Thus the feasibility of a future integrated downstreaming could also be demonstrated. [source] Distribution Patterns of Migrant and Resident Birds in Successional Forests of the Yucatan Peninsula, Mexico,BIOTROPICA, Issue 1 2001Andrea L. Smith ABSTRACT Increasing human activity in the Yucatan peninsula has led to declines in older stages of successional forest, threatening regional habitat diversity. To determine potential effects of this habitat loss on the region's avifauna, we examined the relative use of different forest stages by resident and migrant birds during the nonbreeding season. We used the fixed-width transect method to compare the distribution, abundance, and diversity of forest birds in early (five to ten years old), mid (15,25 years), and late (<50 years) successional forests in the state of Campeche, Mexico, in the south-central part of the peninsula. All stages of successional forest had highly similar bird assemblages and did not differ in bird abundance or diversity. Both migrant and resident birds occurred across the successional gradient. The majority of habitat specialists, however, were resident birds restricted to late-successional forest, indicating that early secondary growth may not be suitable for all species. Furthermore, resident birds that typically participate in mixed-species flocks attained their greatest densities in the oldest forest habitat. Rapid recovery of pre-disturbance physiognomic features, in addition to high levels of habitat connectivity in the region, may contribute to similar bird communities across a range of successional stages. The high degree of edge characterizing much of the forest mosaic also may allow birds access to different serai stages. Loss of late-successional forest, however, is likely to adversely affect the subset of resident avifauna that depends on unique features of mature habitat such as snags, large trees, and climatic buffering. Conservation efforts in Campeche should focus on the specialized requirements of the most habitat-restricted species while preserving the current landscape mosaic characteristic of the small-scale shifting cultivation system. RESUMES El aumento de la actividad humana en el uso del suelo en la Peninsula de Yucatán, ha resultado en la disminución de la regeneración de la selva a etapas más maduras de sucesión, amenazando la diversidad regional de habitats. Para determinar los efectos de esta pérdida sobre la avifauna de la región, estudiamos el uso de diferentes etapas de sucesión de la selva por aves residentes y migratorias durante la temporada no reproductiva. Usamos el método de transecto linear de anchura fija para comparar la distributión, abundancia y diversidad de aves en vegetaclón sucesional (acahuales) de diferentes edades, incluyendo acahual joven (cinco a diez años de edad), acahual de edad media (15-25 afios) y selva (>50 afios) en el sur del estado de Campeche, Mexico. Diferentes etapas de sucesión de la selva tuvieron composition de aves muy similares y no variaron en la abundancia y la diversidad. Tanto las especies migrantes como residentes, estuvieron presentes a travél del gradiente sucesional. Sin embargo, la mayoría de los especialistas fueron aves residentes restingidas a etapas más viejas de sucesión de selva, indicando que la vegetatión sucesional temprana no es favorable para todas las especies. En adicion, las aves residentes que tipicamente participan en bandadas mixtas tuvieron sus densidades más altas en la selva de mayor edad de sucesión. La rápida recuperatión de la selva a sus caracteristicas de estado primario, en adición al alto grado de conectividad de habitat en la región, puede permitir a muchas especies nativas encontrarse en diferentes etapas de vegetatión sucesional. El alto grado de efecto de borde que caracteriza la mayor pane del mosaico de bosque tambíen puede permitir a las aves el acceso a los diferentes tipos de vegetación sucesional. Sin embargo, la pérdida de selva en etapa más madura de sucesión, probablemente rendrá un efecto más adverso sobre el grupo de aves residentes que depende de características únicas de selva madura, tales como árboles secos, árboles grandes y amortiguamiento climático. Los esfuerzos de conservatión deberían enfocarse sobre las especies sensibles con requerimientos especializados de hábitat, preservando selva nativa a la vez que las características actuates de mosaico del paisaje, manteniendo las actividades de roza-tumba y quema a pequena escala. [source] Evaluating the enemies hypothesis in a clover-cabbage intercrop: effects of generalist and specialist natural enemies on the turnip root fly (Delia floralis)AGRICULTURAL AND FOREST ENTOMOLOGY, Issue 2 2010Maria Björkman 1The relative importance of the resource concentration hypothesis and the enemies hypothesis was investigated for the turnip root fly Delia floralis in a cabbage,red clover intercropping system compared with a cabbage monoculture. 2Delia floralis egg densities were measured as well as the activity-densities of generalist predators in a field experiment during two growing seasons. In the second year, a study of egg predation with artificially placed eggs was conducted, in addition to a predator exclusion experiment, to estimate total predation during the season. Parasitization rates were estimated from samples of pupae. 3Delia floralis oviposition was greater in the monoculture during both years. The predator activity-densities differed between treatments and study years. The known natural enemies of Delia spp., Bembidion spp. and Aleochara bipustulata showed a strong response to a cultivation system with higher activity-densities in the monoculture. The response, however, appeared to be caused primarily by habitat preferences and not by D. floralis egg densities. 4The reduction in the number of D. floralis pupae in the intercropping may be explained by a disruption in oviposition behaviour caused by the presence of clover because neither predation, nor parasitization rates differed between cultivation systems. [source] Nutrient losses from rain-fed bench terraced cultivation systems in high rainfall areas of the mid-hills of NepalLAND DEGRADATION AND DEVELOPMENT, Issue 5 2007G. P. Acharya Abstract Between the elevations of 1000 and 2000,m in the mid-hills of Nepal, over 12 million people subsist on land-holdings of less than 0·5,ha. These farmers have limited access to commercial inputs such as fertilisers and are reliant on organic manures for soil fertility maintenance. Participatory research was conducted with farmers on bari land (upper slope rain-fed crop terraces) in the hill community of Landruk (bench terraces 0,5° slope, 3000,3500,mm annual rainfall, which aimed to develop soil and water management interventions that controlled erosion without resulting in high leaching, and so were effective in minimising total nutrient losses. Interventions tested were the control of water movement through diversion of run-on and planting fodder grasses on terrace risers on bench terraces. The interventions were effective in reducing soil loss from the bari land in comparison with existing farmer practices, but no effect was observed on nutrient losses in solution form through runoff and leaching. Losses of NO3 -N in leachate ranged from 17·3 to 99·7,kg,ha,1,yr,1, but only 0·7 to 5·6,kg,ha,1,yr,1 in runoff. The overall nutrient balance suggests that the system is not sustainable. Fertility is heavily dependent on livestock inputs and if the current trends of declining livestock numbers due to labour constraints continue, further losses in productivity can be expected. However, farmers are interested in interventions that tie ecosystem services with productivity enhancement and farmers' priorities should be used as entry points for promoting interventions that are system compatible and harness niche opportunities. Copyright © 2007 John Wiley & Sons, Ltd. [source] Aerobic batch cultivation in micro bioreactor with integrated electrochemical sensor arrayBIOTECHNOLOGY PROGRESS, Issue 1 2010Michiel van Leeuwen Abstract Aerobic batch cultivations of Candida utilis were carried out in two micro bioreactors with a working volume of 100 ,L operated in parallel. The dimensions of the micro bioreactors were similar as the wells in a 96-well microtiter plate, to preserve compatibility with the current high-throughput cultivation systems. Each micro bioreactor was equipped with an electrochemical sensor array for the online measurement of temperature, pH, dissolved oxygen, and viable biomass concentration. Furthermore, the CO2 production rate was obtained from the online measurement of cumulative CO2 production during the cultivation. The online data obtained by the sensor array and the CO2 production measurements appeared to be very reproducible for all batch cultivations performed and were highly comparable to measurement results obtained during a similar aerobic batch cultivation carried out in a conventional 4L bench-scale bioreactor. Although the sensor chip certainly needs further improvement on some points, this work clearly shows the applicability of electrochemical sensor arrays for the monitoring of parallel micro-scale fermentations, e.g. using the 96-well microtiterplate format. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010 [source] | ||||||||||||||||