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Critical Component (critical + component)
Selected AbstractsLocating Critical Components of Regional Human CapitalPOLICY STUDIES JOURNAL, Issue 1 2001Michael C. Farmer Enormous attention has been devoted recently to the contributions of human capital in the strategic and competitive success of different regions. These advances by macroeconomists and trade theorists have important implications for understanding the influence of economic development programs in a rural environment. Drawing upon this research we argue that human capital concepts may offer a more effective basis for assessment of strategic resource needs and use within a rural setting. An approach using direct elicitation methods is outlined for developing human capital measures. One significant advantage of a human capital approach is the potential for creating indicators that bridge the interests of economic development and environmental programs. [source] Glucose sensors: a review of current and emerging technologyDIABETIC MEDICINE, Issue 3 2009N. S. Oliver Abstract Glucose monitoring technology has been used in the management of diabetes for three decades. Traditional devices use enzymatic methods to measure glucose concentration and provide point sample information. More recently continuous glucose monitoring devices have become available providing more detailed data on glucose excursions. In future applications the continuous glucose sensor may become a critical component of the closed loop insulin delivery system and, as such, must be selective, rapid, predictable and acceptable for continuous patient use. Many potential sensing modalities are being pursued including optical and transdermal techniques. This review aims to summarize existing technology, the methods for assessing glucose sensing devices and provide an overview of emergent sensing modalities. [source] Wavelet-based simulation of spectrum-compatible aftershock accelerogramsEARTHQUAKE ENGINEERING AND STRUCTURAL DYNAMICS, Issue 11 2008S. Das Abstract In damage-based seismic design it is desirable to account for the ability of aftershocks to cause further damage to an already damaged structure due to the main shock. Availability of recorded or simulated aftershock accelerograms is a critical component in the non-linear time-history analyses required for this purpose, and simulation of realistic accelerograms is therefore going to be the need of the profession for a long time to come. This paper attempts wavelet-based simulation of aftershock accelerograms for two scenarios. In the first scenario, recorded main shock and aftershock accelerograms are available along with the pseudo-spectral acceleration (PSA) spectrum of the anticipated main shock motion, and an accelerogram has been simulated for the anticipated aftershock motion such that it incorporates temporal features of the recorded aftershock accelerogram. In the second scenario, a recorded main shock accelerogram is available along with the PSA spectrum of the anticipated main shock motion and PSA spectrum and strong motion duration of the anticipated aftershock motion. Here, the accelerogram for the anticipated aftershock motion has been simulated assuming that temporal features of the main shock accelerogram are replicated in the aftershock accelerograms at the same site. The proposed algorithms have been illustrated with the help of the main shock and aftershock accelerograms recorded for the 1999 Chi,Chi earthquake. It has been shown that the proposed algorithm for the second scenario leads to useful results even when the main shock and aftershock accelerograms do not share the same temporal features, as long as strong motion duration of the anticipated aftershock motion is properly estimated. Copyright © 2008 John Wiley & Sons, Ltd. [source] Distribution and dispersal of desert mistletoe is scale-dependent, hierarchically nestedECOGRAPHY, Issue 2 2004Juliann Eve Aukema Spatial patterns are important to many ecological processes, and scale is a critical component of both patterns and processes. I examined the pattern and scale of the spatial distribution of infection of host plants by the desert mistletoe, Phoradendron californicum, in a landscape that spans several square kilometers. I also studied the relationship between mistletoe infection and seed dispersal. I found elevated seed rain in areas with a high prevalence of mistletoes and I found that a greater proportion of trees receive seeds than are infected, suggesting that mistletoes will be aggregated in space. Using nested analysis of variance and variograms, I found that mistletoe infections were distributed in hierarchical patches. Mistletoes were aggregated within trees and mistletoe prevalence was correlated at scales of <1500 m, and at scales >4000 m. Patterns at the largest scales were correlated with elevation: sites at higher elevations showed reduced mistletoe infection compared to those at lower elevations. I propose that at small scales, mistletoe distributions are primarily the result of aggregation of seed-dispersing birds, and that the elevational effect could reflect the recent colonization of higher elevations by the mistletoes' mesquite hosts or the limits of the mistletoes' physiological tolerance to freezing-induced cavitation. [source] Plateau potential-dependent windup of the response to primary afferent stimuli in rat dorsal horn neuronsEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 9 2000Valérie Morisset Abstract In the spinal cord, repetitive stimulation of nociceptive afferent fibres induces a progressive build-up of dorsal horn neuron (DHN) responses. This ,action potential windup' is used as a cellular model of central sensitization to pain. It partly relies on synaptic plasticity, being reduced after blocking NMDA and neurokinin receptors. Using intracellular recordings in a slice preparation of the rat spinal cord, we have analysed the implication of an additional non-synaptic component of windup. Primary afferent fibres were electrically stimulated in the dorsal root. Of 47 responding deep DHNs, 17 (36%) produced action potential windup and afterdischarge during consecutive periods of repeated stimuli (0.4,1 Hz) activating high- (n = 13 neurons) and low-threshold (n = 6 neurons) afferent fibres. When the NMDA receptors were blocked, the rate of windup did not change. In all neurons, there was an absolute correlation between expression of windup and the production of calcium-dependent plateau potentials. Sensitization of the DHN response, similar to the synaptically induced windup, was obtained by repetitive intracellular injection of depolarizing current pulses. This intracellularly induced windup had the same pharmacology as the plateau potential. Synaptically induced windup was also abolished by nifedipine, an L-type calcium-channel blocker. Expression of plateau properties in DHNs is therefore a critical component of windup, operating downstream of synaptic processes. Being associated with calcium influx, generation of plateau potentials could be a link between short-term plasticity and the long-term modification of DHN excitability associated with central sensitization. [source] EVOLUTION AND STABILITY OF THE G-MATRIX ON A LANDSCAPE WITH A MOVING OPTIMUMEVOLUTION, Issue 8 2004Adam G. Jones Abstract In quantitative genetics, the genetic architecture of traits, described in terms of variances and covariances, plays a major role in determining the trajectory of evolutionary change. Hence, the genetic variance-covariance matrix (G-matrix) is a critical component of modern quantitative genetics theory. Considerable debate has surrounded the issue of G-matrix constancy because unstable G-matrices provide major difficulties for evolutionary inference. Empirical studies and analytical theory have not resolved the debate. Here we present the results of stochastic models of G-matrix evolution in a population responding to an adaptive landscape with an optimum that moves at a constant rate. This study builds on the previous results of stochastic simulations of G-matrix stability under stabilizing selection arising from a stationary optimum. The addition of a moving optimum leads to several important new insights. First, evolution along genetic lines of least resistance increases stability of the orientation of the G-matrix relative to stabilizing selection alone. Evolution across genetic lines of least resistance decreases G-matrix stability. Second, evolution in response to a continuously changing optimum can produce persistent maladaptation for a correlated trait, even if its optimum does not change. Third, the retrospective analysis of selection performs very well when the mean G-matrix (,) is known with certainty, indicating that covariance between G and the directional selection gradient (3 is usually small enough in magnitude that it introduces only a small bias in estimates of the net selection gradient. Our results also show, however, that the contemporary ,-matrix only serves as a rough guide to ,. The most promising approach for the estimation of G is probably through comparative phylogenetic analysis. Overall, our results show that directional selection actually can increase stability of the G-matrix and that retrospective analysis of selection is inherently feasible. One ?riajor remaining challenge is to gain a sufficient understanding of the G-matrix to allow the confident estimation of ,. [source] Inactivation of phosphorylase is a major component of the mechanism by which insulin stimulates hepatic glycogen synthesisFEBS JOURNAL, Issue 13 2003Susan Aiston Multiple signalling pathways are involved in the mechanism by which insulin stimulates hepatic glycogen synthesis. In this study we used selective inhibitors of glycogen synthase kinase-3 (GSK-3) and an allosteric inhibitor of phosphorylase (CP-91149) that causes dephosphorylation of phosphorylase a, to determine the relative contributions of inactivation of GSK-3 and dephosphorylation of phosphorylase a as alternative pathways in the stimulation of glycogen synthesis by insulin in hepatocytes. GSK-3 inhibitors (SB-216763 and Li+) caused a greater activation of glycogen synthase than insulin (90% vs. 40%) but a smaller stimulation of glycogen synthesis (30% vs. 150%). The contribution of GSK-3 inactivation to insulin stimulation of glycogen synthesis was estimated to be less than 20%. Dephosphorylation of phosphorylase a with CP-91149 caused activation of glycogen synthase and translocation of the protein from a soluble to a particulate fraction and mimicked the stimulation of glycogen synthesis by insulin. The stimulation of glycogen synthesis by phosphorylase inactivation cannot be explained by either inhibition of glycogen degradation or activation of glycogen synthase alone and suggests an additional role for translocation of synthase. Titrations with the phosphorylase inactivator showed that stimulation of glycogen synthesis by insulin can be largely accounted for by inactivation of phosphorylase over a wide range of activities of phosphorylase a. We conclude that a signalling pathway involving dephosphorylation of phosphorylase a leading to both activation and translocation of glycogen synthase is a critical component of the mechanism by which insulin stimulates hepatic glycogen synthesis. Selective inactivation of phosphorylase can mimic insulin stimulation of hepatic glycogen synthesis. [source] Effects of ontogeny, temperature, and light on vertical movements of larval Pacific cod (Gadus macrocephalus)FISHERIES OCEANOGRAPHY, Issue 5 2009THOMAS P. HURST Abstract The role of behavior, especially vertical migration, is recognized as a critical component of realistic models of larval fish dispersion. Unfortunately, our understanding of these behaviors lags well behind our ability to construct three-dimensional flow-field models. Previous field studies of vertical behavior of larval Pacific cod (Gadus macrocephalus) were limited to small, preflexion stages (,11 mm SL) in a narrow range of thermal conditions. To develop a more complete picture of larval behavior, we examined the effects of ontogeny, temperature, and light on vertical responses of larval Pacific cod in experimental columns. While eggs were strictly demersal, yolk-sac larvae displayed a strong surface orientation as early as 1 day post hatch (, 5 mm SL). Consistent with field observations, small preflexion larvae (<10 mm SL) showed no response to varying light levels. However, there was a direct effect of temperature on larval behavior: Pacific cod larvae exhibited a stronger surface orientation at 4°C than at 8°C. The behavior of larger, postflexion larvae (>15 mm SL) in experimental columns was consistent with a diel vertical migration and independent of water temperature: fish were more widely distributed in the column, and median positions were consistently deeper at higher light levels. These laboratory observations are combined with observations from discrete-depth (MOCNESS) sampling in the Gulf of Alaska to characterize the vertical distribution of larval Pacific cod and contrast ontogenetic patterns with walleye pollock (Theragra chalcogramma). The vertical movements of larval Pacific cod described here will be applied in the development of dispersal projections from Gulf of Alaska spawning grounds. [source] The Orthogonal (e,e,e)-Tris-Adduct of 9,10-Dimethylanthracene with C60 -Fullerene: A Hidden Cornerstone of Fullerene Chemistry.HELVETICA CHIMICA ACTA, Issue 8 2008Preliminary Communication Abstract Tris(9,,10,-dimethyl[9,10]ethanoanthracene[11,,12,:,1,9;11,,12,:,16,17;11,,,,12,,,:,30,31])[5,6]fullerene C60, the orthogonal (e,e,e)-tris-adduct of C60 and 9,10-dimethylanthracene, was obtained from [4+2]-cycloaddition (Diels,Alder reaction) at room temperature. The thermally unstable orange red (e,e,e)-tris-adduct was purified by chromatography and was isolated in the form of red monoclinic crystals. Its C3 -symmetric addition pattern was established spectroscopically. Its structure could be further investigated by single crystal X-ray diffraction. The (e,e,e)-tris-adduct of C60 and 9,10-dimethylanthracene has earlier been suggested as intermediate and reversibly formed critical component in ,template directed' addition reactions of C60. This previously elusive compound has now been isolated and structurally characterized. [source] Lipoxin A4 inhibited hepatocyte growth factor-induced invasion of human hepatoma cellsHEPATOLOGY RESEARCH, Issue 9 2009Xiao-Yan Zhou Aim:, Inflammation is a critical component of tumor progression. Lipoxin A4 (LXA4) has been approved for potent anti-inflammatory properties. Recently, it was reported that LXA4 repressed the expression and activity of cyclooxygenase-2 (COX-2), which is essential for invasion. However, there are few reports dealing with its effects on cancer. To explore whether LXA4 regulate invasion, the effects of LXA4 and its receptor agonist BML-111 on hepatocyte growth factor (HGF)-induced invasion of hepatoma cells and the possible mechanisms were researched. Methods:, Lipoxin A4 receptor (ALX) expression in HepG2 cells were measured through reverse transcription polymerase chain reaction and western blot. Cytotoxicity of LXA4 and BML-111 to HepG2 cells was detected by MTT and (3H)-TdR incorporation assay. Cell migration and invasion assays were performed using a Boyden chemotaxis chamber. COX-2 expression was detected by real-time polymerase chain reaction and western blot, respectively. Moreover, the expressions of matrix metalloproteinases (MMP)-2, MMP-9, I,B, and nuclear factor-,B (NF-,B) p65 were observed via western blot, and NF-,B transcriptional activity was tested by transfections and luciferase activities assay. Results:, ALX expression was detected in HepG2 cells, and suitable concentrations of LXA4 and BML-111 had no cytotoxicity to cells. LXA4 and BML-111 inhibited HGF-induced migration and invasion; downregulated COX-2, MMP-2 and -9; restrained HGF-induced I,B, degradation, NF-,B translocation and the transcriptional activity of NF-,B in HepG2 cells. Furthermore, exogenous PGE2 could reverse the inhibitory effects of LXA4 also BML-111 on HGF-induced invasion and migration partially. Conclusion:, LXA4 inhibited HGF-induced invasion of HepG2 cells through NF-,B/COX-2 signaling pathway partially. [source] When Auditors Err: How Mistake Significance and Superiors' Historical Reactions Influence Auditors' Likelihood to Admit a MistakeINTERNATIONAL JOURNAL OF AUDITING, Issue 1 2010Chad Stefaniak The procedures performed by staff auditors are a critical component of the audit process, and mistakes in these procedures could jeopardize opinions if they are not communicated. While professional standards instruct auditors to report their errors, auditors have incentives to withhold information about mistakes because they are protective of their professional images. These conflicting pressures are examined by investigating the effects of mistake significance and superiors' historical reactions to mistake admissions on the likelihood that staff auditors will admit mistakes. We find an interaction suggesting that staff auditors are more likely to admit errors when their superiors have reacted positively, regardless of error significance. Conversely, staff auditors are less likely to admit apparently insignificant errors when their superiors have reacted negatively to prior mistakes. [source] Caregiver preference for rivastigmine patch relative to capsules for treatment of probable Alzheimer's diseaseINTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY, Issue 5 2007Bengt Winblad Abstract Background Family caregivers comprise a critical component in the care of Alzheimer's disease (AD) patients. Among their many tasks, caregivers are responsible for administering and managing medications. Effective interventions incorporate the needs of both the AD patient and the caregiver, and understanding treatment preferences may maximize intervention effectiveness. Transdermal patches may offer advantages over conventional oral formulations. Methods A 24-week randomized controlled trial compared the rivastigmine patch to the rivastigmine capsule and placebo in patients with probable AD. At baseline and Weeks 8 and 24, the AD Caregiver Preference Questionnaire (ADCPQ) was used to evaluate caregiver expectations, preferences and satisfaction with treatment. Double-dummy treatment blinding ensured that caregiver preference for the patch or capsule was not confounded by perceptions of efficacy or tolerability. Reasons for preference were also elicited. The analytic sample included caregivers who completed the ADCPQ at Weeks 8 and/or 24. Results One thousand and fifty-nine caregivers completed the ADCPQ. More than 70% of caregivers preferred the rivastigmine patch to the capsule. The patch was significantly preferred to the capsule with respect to ease of following the schedule and ease of use. Caregivers indicated greater satisfaction overall, greater satisfaction with administration, and less interference with daily life with the patch versus the capsule (all p,,,0.01). Conclusion Caregivers of AD patients preferred the patch to the capsule for drug delivery. Preference for the rivastigmine patch could potentially lead to improved compliance and improved clinical benefits. Copyright © 2007 John Wiley & Sons, Ltd. [source] Clinical outcome of retroperitoneal lymph node dissection after induction chemotherapy for metastatic non-seminomatous germ cell tumorsINTERNATIONAL JOURNAL OF UROLOGY, Issue 9 2004MOTOTSUGU MURAMAKI Abstract Background: Retroperitoneal lymph node dissection (RPLND) following induction chemotherapy has been considered a critical component in the comprehensive management of advanced non-seminomatous germ cell tumors (NSGCT). The objectives of the present study were to review the clinical outcome of patients who underwent RPLND and to evaluate the probability of necrosis alone, based on some readily available clinical data for these patients. Methods: Forty-seven consecutive patients with NSGCT were treated with first-line chemotherapy at our institution between January 1993 and September 2002. Twenty-four of these patients, who underwent RPLND with normal values of tumor markers after induction chemotherapy, were included in the study. The cause-specific survival rate was calculated using the Kaplan,Meier method. Various predictive factors for the histology were analyzed using multivariate analysis. Results: The pathological findings at resection were necrosis alone in 62.5% of cases, teratoma in 25.0%, and viable cancer in 12.5%. The cause-specific 3-year survival rate of patients who underwent complete and incomplete resection was 100% and 50.0%, respectively. Among several clinical factors, prechemotherapy tumor size less than 50 mm was found to be an independent predictor of necrosis alone (hazard ratio = 4.45, P= 0.04). Conclusion: Metastatic tumor size before chemotherapy appears to be one of the most important factors for the prediction of necrosis alone in the resected specimens of RPLND. The prognosis of patients might be influenced by the degree to which resection has been completed. [source] A Conservative Approach to Performing Transseptal Punctures Without the Use of Intracardiac Echocardiography: Stepwise Approach with Real-Time Video ClipsJOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY, Issue 6 2007ALAN CHENG M.D. Atrial transseptal puncture as a means of accessing the left heart is a critical component of catheter ablation procedures for atrial fibrillation, left-sided accessory pathways, and access to the left ventricle in patients with certain types of prosthetic aortic valves. Although this technique has been performed successfully since the 1950s, severe and potentially life-threatening complications can still occur, including cardiac tamponade and/or death. Some have adopted the use of intracardiac echocardiography, but our laboratory and many others throughout the world have successfully relied on fluoroscopic imaging alone. The aim of this brief report is to describe in detail our technique for performing transseptal punctures during catheter ablation procedures for atrial fibrillation. We employ a similar approach when targeting left-sided accessory pathways, although only a single transseptal is performed in those cases. Utilizing a series of real-time video clips, we describe our technique of double transseptal puncture and illustrate in detail ways in which to avoid common pitfalls. [source] Subnuclear targeting of Runx1 Is required for synergistic activation of the myeloid specific M-CSF receptor promoter by PU.1,JOURNAL OF CELLULAR BIOCHEMISTRY, Issue 4 2005Xiangen Li Abstract Many types of acute myelogenous leukemia involve chromosomal translocations that target the C-terminus of Runx1/AML1 transcription factor, a master regulator of hematopoiesis. The C-terminus of Runx1/AML1 that includes the nuclear matrix targeting signal (NMTS) is essential for embryonic development, hematopoiesis, and target gene regulation. During the onset and normal progression of hematopoiesis, several lineage-specific factors such as C/EBP, and PU.1 interact with Runx1 to regulate transcription combinatorially. Here we addressed the functional interplay between subnuclear targeting of Runx1 and gene activation during hematopoiesis. Point mutations were generated in the NMTS of the human Runx1 protein and tested for their effect on transcriptional cooperativity with C/EBP, and PU.1 at myeloid-specific promoters. We characterized five mutants that do not alter nuclear import, DNA binding or C/EBP,-dependent synergistic activation of the target gene promoters. However a critical tyrosine in the NMTS is required for subnuclear targeting and activation of the granulocyte-macrophage colony stimulating factor (GM-CSF) promoter. Furthermore, this point mutation is defective for transcriptional synergism with PU.1 on the macrophage colony stimulating factor (MCSF) receptor c-FMS promoter. Our results indicate that the NMTS region of Runx1 is required for functional interactions with PU.1. Taken together, our findings establish that subnuclear targeting of Runx1 is a critical component of myeloid-specific transcriptional control. © 2005 Wiley-Liss, Inc. [source] Regulation of angiotensin II-stimulated osteopontin expression in cardiac microvascular endothelial cells: Role of p42/44 mitogen-activated protein kinase and reactive oxygen species,JOURNAL OF CELLULAR PHYSIOLOGY, Issue 1 2001Zhonglin Xie Using spontaneously hypertensive and aortic banded rats, we have shown that expression of myocardial osteopontin, an extracellular matrix protein, coincides with the development of heart failure and is inhibited by captopril, suggesting a role for angiotensin II (ANG II). This study tested whether ANG II induces osteopontin expression in adult rat ventricular myocytes and cardiac microvascular endothelial cells (CMEC), and if so, whether induction is mediated via activation of mitogen-activated protein kinases (p42/44 MAPK) and involves reactive oxygen species (ROS). ANG II (1 ,M, 16 h) increased osteopontin expression (fold increase 3.3±0.34, n,=,12, P,<,0.01) in CMEC as measured by northern analysis, but not in ARVM. ANG II stimulated osteopontin expression in CMEC in a time- (within 4 h) and concentration-dependent manner, which was prevented by the AT1 receptor antagonist, losartan. ANG II elicited robust phosphorylation of p42/44 MAPK as measured using phospho-specific antibodies, and increased superoxide production as measured by cytochrome c reduction and lucigenin chemiluminescence assays. These effects were blocked by diphenylene iodonium (DPI), an inhibitor of the flavoprotein component of NAD(P)H oxidase. PD98059, an inhibitor of p42/44 MAPK pathway, and DPI each inhibited ANG II-stimulated osteopontin expression. Northern blot analysis showed basal expression of p22phox, a critical component of NADH/NADPH oxidase system, which was increased 40,60% by exposure to ANG II. These results suggest that p42/44 MAPK is a critical component of the ROS-sensitive signaling pathways activated by ANG II in CMEC and plays a key role in the regulation of osteopontin gene expression. Published 2001 Wiley-Liss, Inc. [source] Community health practitioner's practice guideline for a changing health care: Korean contributionJOURNAL OF CLINICAL NURSING, Issue 8 2009Il Sun Ko Aims and objectives., The specific aims of the study were (1) to identify community residents' health problems and community health practitioners' activities, (2) to explore community health practitioners' perception of the practice guidelines and (3) to provide recommendations for the development of a new practice guideline in the future. Background., Community health practitioners in Korea are recognised as a critical component of the public health workforce in rural areas. Community health practitioners are registered nurses with six months special training, who have the chief responsibility of delivering primary health care to remote or isolated communities. Although there has been numerous changes in focus of community health practitioners practice over the two decades, community health practitioners guidelines have never been updated since being first developed in 1981. Design., This investigation employed a cross-sectional survey and focus group interview. Methods., The samples included two different groups: 1003 community health practitioners participated in a survey and a group of 12 community health practitioners participated in a focus group interview. A measure of perception of the guideline was developed from Mansfield's work. Goolsby's criteria were revised and used to guide the focus group interview. Results., The participants recognised that the role of community health practitioners is in a process of transition and expect to use well developed guidelines that will allow an appropriate response to the needs of the community. Community health practitioners are generally supportive of practice guidelines although they report various contextual, social and resource barriers to the use of practice guidelines. Finally, the researchers have provided recommendations for the development of new community health practitioners practice guidelines. Conclusion., A newly developed community health practitioners guideline should assist in articulating new roles and responsibilities in the practice of community health practitioners and establish a foundation for knowledge, skills and training necessary for them to work independently. Relevance to clinical practice., New services made available for under-recognised health problems may be a direct outcome of newly developed guidelines. [source] Short-term prediction of motorway travel time using ANPR and loop dataJOURNAL OF FORECASTING, Issue 6 2008Yanying LiArticle first published online: 28 MAY 200 Abstract Travel time is a good operational measure of the effectiveness of transportation systems. The ability to accurately predict motorway and arterial travel times is a critical component for many intelligent transportation systems (ITS) applications. Advanced traffic data collection systems using inductive loop detectors and video cameras have been installed, particularly for motorway networks. An inductive loop can provide traffic flow at its location. Video cameras with image-processing software, e.g. Automatic Number Plate Recognition (ANPR) software, are able to provide travel time of a road section. This research developed a dynamic linear model (DLM) model to forecast short-term travel time using both loop and ANPR data. The DLM approach was tested on three motorway sections in southern England. Overall, the model produced good prediction results, albeit large prediction errors occurred at congested traffic conditions due to the dynamic nature of traffic. This result indicated advantages of use of the both data sources. Copyright © 2008 John Wiley & Sons, Ltd. [source] Characteristics of sex-related homicides in AlaskaJOURNAL OF FORENSIC NURSING, Issue 2 2010FNP-C, SANE-A, SANE-P, Tara Henry MSN Abstract The identification and interpretation of anogenital findings postmortem is a critical component of a sex-related homicide investigation. The use of a colposcope to assist in identifying anogenital injuries in living sexual assault victims is well established. The use of a colposcope for postmortem anogenital examination has been briefly mentioned in a few publications, however, no studies were found regarding the types and sites of postmortem anogenital injuries identified with a colposcope in sex-related homicide cases. The purpose of this study was to describe the demographic, physical examination, and victim-suspect relationship characteristics of sex-related homicides in Alaska. Genital findings in living and deceased sexual assault victims in Alaska were compared. Implications: Given the results of this study, postmortem sexual assault examinations should be conducted in all suspected intimate partner homicides. Further implications for forensic nursing practice and recommendations for future research are discussed. [source] Implementation of a patient-friendly medication schedule to improve patient safety within a healthcare systemJOURNAL OF HEALTHCARE RISK MANAGEMENT, Issue 4 2010Jodi E. Fredericks PharmD Preventable adverse drug events have a direct impact on the well-being of patients. The creation and implementation of a patient-friendly daily medication schedule improved the way care is delivered at Memorial Healthcare System. The staff collaborated with patients and families and empowered them with the knowledge and tools needed to make their healthcare safer. Patient and family participation, a critical component of patient- and family-centered care, is a vital part of making healthcare safer. This tool enhances communication with patients and family members and enables patients to better understand the medications they receive while hospitalized. An additional welcomed byproduct is the prevention of potential medication errors. [source] Ro5-4864, a peripheral benzodiazepine receptor ligand, reduces reactive gliosis and protects hippocampal hilar neurons from kainic acid excitotoxicityJOURNAL OF NEUROSCIENCE RESEARCH, Issue 1 2005Sergio Veiga Abstract The peripheral-type benzodiazepine receptor (PBR) is a critical component of the mitochondrial permeability transition pore, which is involved in the regulation of cell survival. Different forms of brain injury result in induction of the expression of the PBR in the areas of neurodegeneration, mainly in reactive glial cells. The consequences of induction of PBR expression after brain injury are unknown. To test whether PBR may be involved in the regulation of neuronal survival after injury, we have assessed the effect of two PBR ligands, Ro5-4864 and PK11195, on neuronal loss induced by kainic acid in the hippocampus. Systemic administration of kainic acid to male rats resulted in the induction of a reactive phenotype in astrocytes and microglia and in a significant loss of hilar neurons in the dentate gyrus. Administration of Ro5-4864, before the injection of kainic acid, decreased reactive gliosis in the hilus and prevented hilar neuronal loss. In contrast, PK11195 was unable to reduce reactive gliosis and did not protect hilar neurons from kainic acid. These findings suggest that the PBR is involved in control of neuronal survival and gliosis after brain injury and identify this molecule as a potential target for neuroprotective interventions. © 2005 Wiley-Liss, Inc. [source] Substantive-level theory of highly regarded secondary biology teachers' science teaching orientationsJOURNAL OF RESEARCH IN SCIENCE TEACHING, Issue 2 2005Patricia Meis Friedrichsen Science teaching orientations, defined as teachers' knowledge and beliefs about the purposes and goals for teaching science, have been identified as a critical component within the proposed pedagogical content knowledge (PCK) model for science teaching. Because of the scarcity of empirical studies in this area, this case study examined the nature and sources of science teaching orientations held by four highly regarded secondary biology teachers. Data sources consisted of transcripts from four interviews, a card-sorting task, and classroom observations. Using a grounded theory framework, inductive data analysis led to the construction of a substantive-level theory for this group of participants. In regard to the nature of science teaching orientations, the use of central and peripheral goals, as well as the means of achieving these goals, was used to represent the complex nature of participants' science teaching orientations. The participants' science teaching orientations included goals related to general schooling, the affective domain, and subject matter, although the latter was not always a central component. In regard to the sources of teaching orientations, participants were strongly influenced by the classroom context and their beliefs about learners and learning; additional influences included prior work experiences, professional development, and time constraints. © 2005 Wiley Periodicals, Inc. J Res Sci Teach 42: 218,244, 2005 [source] Alcohol Suppresses IL-2,Induced CC Chemokine Production by Natural Killer CellsALCOHOLISM, Issue 9 2005Ting Zhang Background: Natural killer (NK) cells are a critical component of the host innate immune system. We investigated whether alcohol impairs NK cell function, particularly production of CC chemokines induced by interleukin (IL)-2, the natural ligands for CCR5 receptor. Methods: Primary NK cells and NK cell line (YTS) were cultured with or without alcohol (10 to 80 mM) for three hours. The culture supernatants were then harvested and used to treat human peripheral blood monocyte-derived macrophages and a HeLa cell line, which expresses CD4, CCR5, and CXCR4 receptors (MAGI cells). CC chemokine expression by YTS and primary NK cells treated with or without alcohol was analyzed with the real-time RT-PCR and ELISA. Ca2+i and Western blot assays were used to determine calcium-mediated intracellular signaling pathway and NF-,B p65 expression. HIV strains (Bal and UG024) were used to infect macrophages and MAGI cells. In addition, ADA (macrophage-tropic strain) and murine leukemia virus (MLV) envelope-pseudotyped HIV infection was carried out in macrophages. HIV infectivity was determined by HIV reverse transcriptase (RT) and ,-galactosidase activity assays. Results: Alcohol inhibited IL-2,induced CC chemokine (CCL3 and CCL4) expression by NK cells. Functional tests demonstrated that this reduced expression of CC chemokines was associated with diminished anti-HIV ability of NK cells. Alcohol also reduced the ability of NK cells to response to CCL3-mediated chemotaxis. Alcohol inhibited IL-2,induced NF-,B p65 protein expression and calcium mobilization by NK cells. Conclusions: Alcohol, through the inhibition of IL-2,induced NF-,B p65 protein expression and intracellular calcium mobilization, suppressed NK cell production of CC chemokines. This suppression of CC chemokine production was associated with diminished anti-HIV activity of NK cells. Thus, by inhibiting NK cell,mediated innate immunity against HIV, alcohol consumption may have a cofactor role in the immunopathogenesis of HIV disease. [source] Hippocampal adult neurogenesis is enhanced by chronic eszopiclone treatment in ratsJOURNAL OF SLEEP RESEARCH, Issue 3 2010MELVI METHIPPARA Summary The adult hippocampal dentate gyrus (DG) exhibits cell proliferation and neurogenesis throughout life. We examined the effects of daily administration of eszopiclone (Esz), a commonly used hypnotic drug and ,-aminobutyric acid (GABA) agonist, compared with vehicle, on DG cell proliferation and neurogenesis, and on sleep,wake patterns. Esz was administered during the usual sleep period of rats, to mimic typical use in humans. Esz treatment for 7 days did not affect the rate of cell proliferation, as measured by 5-bromo-2,-deoxyuridine (BrdU) immunostaining. However, twice-daily Esz administration for 2 weeks increased survival of newborn cells by 46%. Most surviving cells exhibited a neuronal phenotype, identified as BrdU,neuronal nuclei (NeuN) double-labeling. NeuN is a marker of neurons. Non-rapid eye movement sleep was increased on day 1, but not on days 7 or 14 of Esz administration. Delta electroencephalogram activity was increased on days 1 and 7 of treatment, but not on day 14. There is evidence that enhancement of DG neurogenesis is a critical component of the effects of antidepressant treatments of major depressive disorder (MDD). Adult-born DG cells are responsive to GABAergic stimulation, which promotes cell maturation. The present study suggests that Esz, presumably acting as a GABA agonist, has pro-neurogenic effects in the adult DG. This result is consistent with evidence that Esz enhances the antidepressant treatment response of patients with MDD with insomnia. [source] Linguistic politeness and face-work in computer-mediated communication, Part 1: A theoretical frameworkJOURNAL OF THE AMERICAN SOCIETY FOR INFORMATION SCIENCE AND TECHNOLOGY, Issue 13 2008Jung-ran Park Our daily social interaction is anchored in interpersonal discourse; accordingly, the phenomenon of linguistic politeness is prevalent in daily social interaction. Such linguistic behavior underscores the fact that linguistic politeness is a critical component of human communication. Speech participants utilize linguistic politeness to avoid and reduce social friction and enhance each other's face, or public self-image, during social interaction. It is face-work that underlies the interpersonal function of language use and encompasses all verbal and nonverbal realizations that bring forth one's positive social value, namely, face. Face-work is founded in and built into dynamic social relations; these social and cultural relations and context directly affect the enactment of face-work. Analysis and a subsequent understanding of sociointerpersonal communication are critical to the fostering of successful interaction and collaboration. Linguistic politeness theory is well positioned to provide a framework for an analysis of social interaction and interpersonal variables among discourse participants inasmuch as it is applicable not only to face-to-face social interactions but also to those interactions undertaken through online communication. [source] Critical Evaluation of How the Rosgen Classification and Associated "Natural Channel Design" Methods Fail to Integrate and Quantify Fluvial Processes and Channel Response,JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION, Issue 5 2007A. Simon Abstract:, Over the past 10 years the Rosgen classification system and its associated methods of "natural channel design" have become synonymous to some with the term "stream restoration" and the science of fluvial geomorphology. Since the mid 1990s, this classification approach has become widely adopted by governmental agencies, particularly those funding restoration projects. The purposes of this article are to present a critical review, highlight inconsistencies and identify technical problems of Rosgen's "natural channel design" approach to stream restoration. This paper's primary thesis is that alluvial streams are open systems that adjust to altered inputs of energy and materials, and that a form-based system largely ignores this critical component. Problems with the use of the classification are encountered with identifying bankfull dimensions, particularly in incising channels and with the mixing of bed and bank sediment into a single population. Its use for engineering design and restoration may be flawed by ignoring some processes governed by force and resistance, and the imbalance between sediment supply and transporting power in unstable systems. An example of how C5 channels composed of different bank sediments adjust differently and to different equilibrium morphologies in response to an identical disturbance is shown. This contradicts the fundamental underpinning of "natural channel design" and the "reference-reach approach." The Rosgen classification is probably best applied as a communication tool to describe channel form but, in combination with "natural channel design" techniques, are not diagnostic of how to mitigate channel instability or predict equilibrium morphologies. For this, physically based, mechanistic approaches that rely on quantifying the driving and resisting forces that control active processes and ultimate channel morphology are better suited as the physics of erosion, transport, and deposition are the same regardless of the hydro-physiographic province or stream type because of the uniformity of physical laws. [source] Evaluation of Reduced Fish Meal Diets for Second Year Growout of the Largemouth Bass, Micropterus salmoidesJOURNAL OF THE WORLD AQUACULTURE SOCIETY, Issue 6 2009Nathan J. Cochran Development of efficient cost-effective diets is a critical component in the refinement of production technologies for the largemouth bass, Micropterus salmoides (LMB). One of the first steps in reducing feed costs can be to decrease the amount of fish meal in the diet. The objective of this study was to evaluate reduced levels of fish meal, and a least-cost formulation diet, for second year growout of LMB under practical pond conditions. Twelve 0.04-ha ponds were stocked with juvenile LMB (210.1±3.3 g) at a stocking density of 8650 fish/ha (350 fish/pond). Each pond was randomly assigned one of the four dietary treatments with three replicate ponds per treatment. The three experimental diets contained varying levels of fish meal. Diets FM-45, FM-24, and FM-8 contained 45, 23.5, and 8% fish meal, respectively. In diets FM-24 and FM-8, fish meal was replaced by varying levels of poultry by-product meal, soybean meal, and blood meal. The fourth diet was a commercial salmonid diet widely used as a LMB growout feed (Nelson and Sons, Inc., SilvercupTM, Steelhead, Murray, UT, USA). This diet served as a commercial control (CC) and contained 46% crude protein. The experimental diets were formulated to be isonitrogenous and isocaloric with the CC diet and were fed once daily to apparent satiation for 180 d. At harvest, there were no significant differences between treatments (P > 0.05) in terms of survival, which averaged 95% overall. Mean weights of fish fed the three experimental diets FM-45, FM-24 and FM-8 were not significantly different (P > 0.05) and averaged 518, 546, and 529 g, respectively, but were all significantly greater (P, 0.05) than those fed the CC (488 g). Feed conversion ratio (FCR) of fish fed the FM-45 and FM-8 diets (1.43 and 1.46, respectively) was significantly greater (P, 0.05) than those fed the FM-24 diet (1.34). The FCR of fish fed the CC diet (1.39) was not significantly different (P > 0.05) from fish fed other diets. Feed cost per unit of weight gain ($US/kg) was significantly lower (P, 0.05) in fish fed the FM-24 and FM-8 diets ($0.73 and $0.72/kg, respectively) than in fish fed other diets. Feed cost per unit gain of fish fed the FM-45 diet ($0.83/kg) was significantly lower (P, 0.05) than those fed the CC diet ($1.04/kg). There were no significant differences (P > 0.05) in dress-out percentages or proximate composition among fish fed the four diets. This study indicates that fish meal levels in feeds used for the second year growout of LMB can be reduced to,8% of the formulation without reducing survival or growth and without negatively impacting body composition. [source] Swimming speed and foraging strategies of New Zealand sea lions (Phocarctos hookeri)JOURNAL OF ZOOLOGY, Issue 2 2001D. E. Crocker Abstract Lactating New Zealand sea lions (Phocarctos hookeri) have recently been reported to be the longest and deepest-diving otariid. An unusually large proportion of dives exceeded a theoretical aerobic dive limit, predicted from estimated oxygen stores and measurements of diving metabolic rate. We investigated swimming speed, a key variable in both the management of oxygen stores and foraging strategies, and its relation to diving behaviour in New Zealand sea lions. Diving behaviour was nearly continuous with short inter-dive intervals. Mean diving swimming speeds ranged from 1.6 to 2.4 m/s. Mean surface swimming speeds ranged from 0.9 to 1.8 m/s and were significantly lower than diving speeds in all subjects. New Zealand sea lions spend significant but variable amounts of time resting at the surface. Diving and swimming speed patterns were consistent with foraging on the benthos. Time in the foraging zone was maintained in deeper dives by increasing dive duration. This increased duration cannot be accounted for by a decreased metabolic rate resulting from slower swimming speeds, as speeds increased with the maximum depth of dives. Patterns of swimming speed and acceleration suggest the use of a gliding phase during descent. For most females, the extended duration of deeper dives did not impact on surface times, suggesting the use of aerobic metabolism. Females exhibited significantly slower swim speeds during the bottom segments of foraging dives than during descent or ascent. These findings suggest that swimming behaviour should be considered a critical component when modelling energetic costs for diving animals. [source] Influences of milk components on biofilm formation of Cronobacter spp. (Enterobacter sakazakii)LETTERS IN APPLIED MICROBIOLOGY, Issue 6 2009G.I. Dancer Abstract Aim:, To determine the critical component(s) of skim milk for biofilm formation of Cronobacter species. Methods and Results:, Biofilm forming ability of 72 Cronobacter strains in skim milk preparation was assayed by crystal violet staining. The results revealed that whey protein and casein are more important determinants of skim milk for biofilm formation than lactose, although there was a wide variation in biofilm forming ability. Biofilm structure and capsular material of six strains exhibiting different biofilm forming ability was investigated via electron microscopes. Scanning electron microscopy showed visually that while the strong biofilm formers (E27B, FSM 30 and 2·82) resulted in almost complete coagulation of skim milk, the weak biofilm formers (55, FSM 290 and 2·84) caused less coagulation. No capsule was clearly delineated in transmission electron micrographs of either strong or weak biofilm formers. Conclusion:, These results indicate that, for biofilm formation of Cronobacter species in skim milk, nitrogen source is probably a more important determinant than carbohydrate, and that strong biofilm formers are responsible for substantial coagulation of skim milk. Significance and Impact of the Study:, This study provides information for better understanding of the underlying mechanisms by which Cronobacter species form biofilm in infant formula milk. [source] The Internal Israeli Conflict: The Past, Present, and Future of the Jewish West Bank and Gaza SettlementsNEGOTIATION JOURNAL, Issue 2 2005Robert Mnookin On October 14 and 15, 2004, just days before the Israeli government submitted to the Knesset a draft legislation to authorize the evacuation of Jewish settlers from Gaza Strip and some settlements on the West Bank, a two-day conference titled "Past, Present, and Future of the Jewish West Bank and Gaza Settlements: The Internal Israeli Conflict" was held at Harvard Law School. The conference was sponsored by the Program on Negotiation at Harvard Law School, the Saltman Center for Conflict Resolution of the University of Nevada, Las Vegas, and the United States Institute of Peace. This interdisciplinary conference's six panels, whose proceedings are summarized in the series of articles that follow, explored the religious, ideological, psychological, political, legal, and international dimensions of the conflict. Presenters included former and current Israeli and American government officials, experts on resettlement policies and compensation mechanisms, and scholars from a variety of disciplines. While presentation topics covered a range of issues relating to the settlements, three broad themes arose from the conference. First, participants agreed that it is important, if not fundamental, to understand the perspectives of the national religious settlers who are the driving force behind the settlement movement. Exploring the settlers' diverse interests, fears, and identities is necessary in order to see why relocation is so threatening to them. The Israeli government can lessen opposition to withdrawal by showing the settlers empathy and reassurance, but only if government officials first achieve a true understanding of the settlers' concerns. Participants also argued that a reframing of the relocation in ideological terms could be another critical component of a solution to this problem. It may be necessary for the leaders of the settlement movement to develop a new narrative or modify the existing one in order to legitimize their relocation. Part of this narrative will involve the concept of "a greater good", the government must reassure the settlers that their sacrifice is for a higher cause. Several participants noted that Israel needs to show the settlers "tough love." When the relocations begin, many expect that there will be violence and that disturbing images will be broadcast throughout Israel and around the world. Internal disruption could put the government led by Prime Minister Ariel Sharon and his Likud party coalition in jeopardy. The government must not waver in the face of this crisis, conference participants argued. In fact, the threat of violent and disruptive resistance by settlers and their allies can be part of the solution, not just the problem. The government and relocation supporters can use this extremism to justify decisive measures and to redefine the problem for the broader population to convince them that the stability of the country is at stake. Another major conclusion of conference participants was that, while the Israeli settlement issue has unique features, there is much to be learned from comparative analysis. Other countries have dealt with settlement situations, and their experiences offer invaluable lessons. In particular, participants contrasted Israel's settlements in Gaza and the West Bank with French settlements in Algeria and English settlements in Ireland. Some pointed to the French withdrawal from Algeria, which was politically painful but ultimately successful, as an example of "tough love" that Israel should follow. Finally, the involvement of third parties to help solve this conflict is indispensable. Participants noted that while much of Israel feels alienated from the European Union and the United Nations, the Israeli government is highly sensitive to the concerns of the United States, as evidenced by Sharon's decision to show the Gaza withdrawal plan to the U.S. government before he had even raised it with his cabinet and the Israeli parliament, the Knesset. International participation could help legitimize withdrawal and reduce Israeli responsibility for Gaza's future. Third parties can apply political pressure to encourage an accountable and responsible Palestinian leadership. They may also be called upon to provide some sort of financial aid. The participants acknowledged the complexity of the settlement problem and recognized that easy solutions do not exist. Yet, if the Israeli government works toward understanding the settlers' perspectives, learns from comparative analysis, and involves third parties appropriately, the likelihood of a successful outcome increases greatly. [source] | |||||||||||||||||||||||||||||||||||||||||||||||||||||||