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Adaptation Period (adaptation + period)
Selected AbstractsSurvival of sea-water-adapted trout, Salmo trutta L. ranched in a Danish fjordFISHERIES MANAGEMENT & ECOLOGY, Issue 4 2000S. S. Pedersen The effect of seawater adaptation on the survival of coastally released post-smolt trout, Salmo trutta L., was investigated by release: (1) directly (with no adaptation); (2) after retention in net pens in the sea for 29,131 days (delayed release); (3) after feeding with a high-salt diet (12,13.5% NaCl) for 4 weeks; and (4) after a combination of (2) and (3). In total, 17 640 trout (age = 1+, 1.5 and 2+ years; mean fork lengths = 18.2,25.6 cm) were released in 14 batches in the summer or autumn months of 1986,1989. All fish were of domesticated origin and Carlin tagged. Survival and instantaneous mortality rates (total and fishing mortality) were estimated from reported recaptures. Mortality rates were estimated for: (1) the post-smolt period; (2) the period until the legal size of capture (40 cm) was attained; and (3) for larger sea-trout. Release with a delay of 4 weeks gave an increased survival rate. A longer adaptation period did not increase survival. On average, survival was increased by 36%. Survival was not increased by high-salt diets. Until attainment of the legal size for capture, survival was 9.6% higher on average, with extremes as low as 1.7% and as high as 38% in individual batches. [source] Dietary amino acids fed in free form or as protein do differently affect amino acid absorption in a rat everted sac modelJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 5 2008J. A. Nolles Summary In the present study, the effect of free amino acid (FAA) diets on the intestinal absorption rate of methionine and leucine was studied ,ex vivo' with rats adapted for different periods of time to the diets, using the everted sac method. The adaptation period to the 21% FAA diet with an amino acid content based on casein was either, 0 (no adaptation, N-ADA), 5 (short-term adaptation, ST-ADA), or 26,33 days (long-term adaptation, LT-ADA). Within the ST-ADA and the LT-ADA groups, three different levels of methionine were included: 50%, 100% and 200% of the level normally present in casein. All diets were iso-nitrogenous and iso-caloric. After the adaptation period (0, 5, or 26,33 days), intestinal everted sacs were prepared. Methionine or leucine was added to the medium as transport substrate. The methionine absorption rate of the rats of the LT-ADA groups was higher than that of the N-ADA groups. Furthermore, adaptation to 200% dietary methionine levels caused a significantly slower leucine absorption compared to the 100%, and 50% group. Methionine absorption was similar in the 100% and 200% groups, but the absorption of methionine in the 50% group was enhanced in the distal part of the intestines. We concluded that in response diets with 21% FAAs as only amino acid source, amino acid absorption is decreased to avoid toxic effects of high levels of methionine in the circulation. [source] Effects of oat processing on the glycaemic and insulin responses in horsesJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 3-4 2003I. Vervuert Summary This study was conducted to evaluate the effects of different oat processing techniques on the plasma glucose and insulin response in horses. In a cross-over design, six horses (ages 4,15 years, mean body weight ± SD: 450 ± 37 kg) were fed in random order: untreated oats, finely ground, steam-flaked and popped oats. The total oat intake varied between 1.05,1.5 kg/day, and the amount of diet was adjusted to a starch content of 630 g starch per day and horse (1.2,1.5 g starch/kg BW/day). During the stabilization period of 10 days, horses additionally received 6 kg grass hay. Following this adaptation period, plasma glucose and insulin responses to the respective dietary treatments were tested. Horses were fed their test diet (exclusively oats), and blood samples were collected at defined times to determine glycaemic and insulin response. Oat feeding resulted in a significant increase in mean plasma glucose and insulin concentration. However, glucose and insulin peaks as well as their respective areas under the curves were not clearly influenced by oat processing. The glycaemic index varied between 94.7 ± 11.2% (steam-flaked oats) and 102.6 ± 14.5% (finely ground oats, n.s.), the insulin index ranged between 93.8 ± 18.9% (popped oats) and 150.0 ± 77.6% (finely ground oats, n.s.). The insulin reaction to oat feeding showed a high variability between the horses. The results of this study indicate that the glucose and insulin responses are not clearly altered by the different types of oat processing. However, the glucose and insulin responses tended to be lower in thermally treated oats when compared with untreated or finely ground oats. [source] In vitro studies on the effects of Saccharomyces boulardii and Bacillus cereus var. toyoi on nutrient transport in pig jejunumJOURNAL OF ANIMAL PHYSIOLOGY AND NUTRITION, Issue 1-2 2000G. Breves The probiotics Saccharomyces boulardii and Bacillus cereus var. toyoi are nonpathogenic microbes which have been shown to affect certain functions of the mucosal barrier in pig jejunum such as electrogenic ion transport capacity and paracellular permeability. The present studies were performed to investigate potential effects of the probiotics on jejunal nutrient transport such as sodium-dependent glucose transport or proton-dependent dipeptide transport. For this purpose the in vitro Ussing-chamber technique was applied in order to examine net electrogenic ion flux rates (short circuit currents, Isc) across isolated intact jejunal epithelia in the absence and presence of either 10 mmol/l glucose (mucosal side) or two-fold application of 5 mmol/l glycyl- l -sarcosine or glycyl- l -glutamine to the mucosal bathing solution. Brush border membrane vesicles (BBMV) were prepared in order to characterize kinetic parameters (Vmax, Km) of Na-dependent glucose transport. Intestinal tissues were obtained from growing pigs in a weight range between 23 and 33 kg. All animals were fed twice daily and received 0.8,0.9 kg/day of a standard diet. After a 9- to 10-day adaptation period the diets for treated animals were either supplemented for 8 days with 1.7×107 colony-forming units (CFU)/g feed of S. boulardii or for 3 weeks with 106 CFU/g feed B. cereus var. toyoi. Under basal conditions Isc values were not affected by different treatment protocols (controls: 0.74 ± 0.04 µeq/cm2 per h, n=9; S. boulardii: 0.74 ± 0.12 µeq/cm2 per h, n=7; B. cereus 0.68 ± 0.09 µeq/cm2 per h, n=5). Irrespective of dietary treatment, the addition of glucose resulted in significant increases of Isc indicating substantial onset of electrogenic net Na/glucose cotransport. Maximal Isc values occurred within 30 min and reached 2.79 ± 0.41 µeq/cm2 per h in control epithelia. This was significantly lower than found in S. boulardii (4.47 ± 0.43 µeq/cm2 per h, p < 0.05) and B. cereus var. toyoi tissues (4.45 ± 0.31 µeq/cm2 per h, p < 0.05). Gt values were 22.4 ± 1.3 mS/cm2 in control animals and were significantly lower as shown in S. boulardii (p < 0.01) and B. cereus var. toyoi (p < 0.01)-treated animals (28.4 ± 1.3 and 29.9 ± 0.8 mS/cm2, respectively). Vmax values of Na-dependent glucose uptake into BBMV differed significantly between controls (0.64 ± 0.08 nmol/mg protein per 10 s; n=5), S. boulardii (0.89 ± 0.06 nmol/mg protein per 10 s; n=5, p < 0.05) and B. cereus var. toyoi preparations (1.08 ± 0.05 nmol/mg protein per 10 s; n=3, p < 0.01). Km values were not significantly affected (control: 0.31 ± 0.04 mmol/l, S. boulardii: 0.29 ± 0.05 mmol/l, B. cereus var. toyoi: 0.21 ± 0.01 mmol/l). Irrespective of dietary treatment, application of the dipeptide model substances glycyl- l -sarcosine or glycyl- l -glutamine resulted in significant increases of Isc indicating marked stimulation of electrogenic net H+/dipeptide cotransport. Highest Isc responses occurred in B. cereus var. toyoi preparations and lowest were found in control tissues. However, these differences were not significant. Gt values were not affected by different dietary treatments. The results clearly demonstrate that oral administration of either S. boulardii or B. cereus var. toyoi stimulates Na-dependent glucose absorption in pig jejunum. [source] Detection of improvement in the masticatory function from old to new removable partial dentures using mixing ability testJOURNAL OF ORAL REHABILITATION, Issue 9 2005A. ASAKAWA summary, The aim of this study was to determine the sensitivity of the Mixing Ability Test to detect improvement of masticatory function in subjects on transition from old to new removable partial dentures. Thirty-two subjects (seven males, 25 females, mean age 65·0 years) with distal extension partially edentulous area in mandible and/or maxilla participated in the study. The following reasons were presented for replacing the old removable partial dentures with new ones: fracture and/or poor fitness of retainers, extraction of abutment teeth, poor fitness of denture base, severe wear of artificial teeth and request for metal base dentures. Masticatory function with old and new removable partial dentures after an adaptation period (mean 27·4 weeks) was evaluated by the Mixing Ability Test. Subjects were asked to masticate five two-coloured wax cubes with each removable partial denture. Mixing Ability Index was obtained from the colour mixture and shape of the masticated cubes. Wilcoxon signed-rank test was used to test the difference of Mixing Ability Indexes between old and new removable partial dentures. The mixing ability indexes with new removable partial dentures (mean ± s.d.: 0·70 ± 0·68) was significantly higher (P < 0·001) than those with old removable partial dentures (,0·11 ± 1·13). The results suggest that the Mixing Ability Test was capable of detecting improvement in masticatory function with new removable partial dentures. [source] In Vitro Determination of Generation Times for Entodinium exiguum, Ophryoscolex purkynjei and Eudiplodinium maggiiTHE JOURNAL OF EUKARYOTIC MICROBIOLOGY, Issue 3 2004BURK A. DEHORITY ABSTRACT. Most previously reported generation times for rumen ciliate protozoa are longer than would be required to prevent their being flushed out of the rumen. In an earlier study from this lab, using a sequential transfer procedure, generation times between 12 and 13 h were determined for both Epidinium caudatum and Entodinium caudatum. This would permit these species to be maintained in a rumen with a fluid volume turnover rate as rapid as twice a day. In this study, generation times were estimated for Entodinium exiguum (13.2 h), Eudiplodinium maggii (26.8 h), and Ophryoscolex purkynjei (29 h), by sequential transfer at both 12 and 24 h time periods. The generation time for E. exiguum is lower than reported for this and other Entodinium species as determined by logarithmic growth from a small inoculum, but similar to that obtained for Ent. caudatum using sequential transfer. Eudiplodinium maggii and O. purkynjei generation times are similar to previous estimates of 24- and 24,48 h, respectively. However, it was observed that after an adaptation period of 36 to 48 h (generally 3,4 transfers) cell concentrations decreased and generation times were markedly decreased, i.e. 12.2 h for Ent. exiguum, 15.0 h for E. maggii and 12.8 h for O. purkynjei. In a separate study, varying both the concentration of Epidinium and the quantity of substrate fed per cell had no effect on generation time. [source] Reduction of ammonia emission from growing pig rooms by feeding a lower protein diet supplemented with apple pomaceANIMAL SCIENCE JOURNAL, Issue 6 2002Akemi YAMAMOTO ABSTRACT An experiment was conducted to determine the effect of supplementing a reduced crude protein (CP) diet with apple pomace on the ammonia emissions from growing pig rooms. Four pigs (45 kg BW) each were assigned to one of two diets. Each group was housed in a separate room and fed a standard diet (CP 16.6%) or a low CP, amino acid-supplemented diet (CP 9.1%) containing 23.1% of dried apple pomace for two 7-day experimental periods. After the completion of the first period, the pigs were switched to the other diet. The daily ammonia emissions, measured for 3 days after a 4-day adaptation period, were much lower for pigs fed the apple pomace-supplemented diet than for pigs fed the standard diet (0.47 g/pig vs 7.30 g/pig, respectively). The daily nitrogen intake for the standard diet and the apple pomace-supplemented diet was 58.1 and 35.5 g/pig, respectively. The pigs fed the apple pomace-supplemented diet excreted more fecal nitrogen than pigs fed the standard diet (17.5 g/day vs 11.0 g/day, respectively), but urinary nitrogen excretion with the apple pomace-supplemented diet was estimated to be 2.9 g/day, which was much lower than that for the standard diet (27.0 g/day). The addition of apple pomace to a reduced CP, amino acid-supplemented diet reduces urinary nitrogen excretion and thereby ammonia emission. [source] Dietary fish oil impairs induction of ,-interferon and delayed-type hypersensitivity during a systemic Salmonella enteritidis infection in ratsAPMIS, Issue 8 2010Johannes Snel Snel J, Born L, van der Meer R. Dietary fish oil impairs induction of ,-interferon and delayed-type hypersensitivity during a systemic Salmonella enteritidis infection in rats. APMIS 2010; 118: 578,84. Fish oil that is rich in n-3 polyunsaturated fatty acids markedly modulates immunological responses. Literature data indicate that the fish oil reduces cellular immunity and therefore impairs resistance to infections. We have investigated how dietary fish oil affects the immune response against a facultative intracellular pathogen, Salmonella enteritidis. Wistar rats were fed a diet containing 16% (w/w) of either fish oil or corn oil. After a 4-week adaptation period, rats were intraperitoneally challenged with 4 × 105 cfu of S. enteritidis. During the 14-day infection period, urine was collected on a daily basis. At days 2 and 14, eight rats per group were sacrificed. Urinary nitrate, used as a marker for NO production, was lower on a fish oil diet during days 3,8. At day 2, serum ,-interferon was 48 ± 7 pg/mL in the fish oil-fed rats compared with 162 ± 52 pg/mL in the corn oil-fed rats. No effects were found on living salmonella in liver and spleen. At day 14, as markers of an impaired T-helper 1 (Th-1) response, a 38% lower delayed-type hypersensitivity responses and a lower salmonella-specific IgG2b were observed in the fish oil-fed rats. Although here dietary fish oil has affected only immune parameters, this impairment of the innate and Th-1-mediated immune response may have implications for the host resistance against other intracellular pathogens. [source] Speech adaptation after treatment of full edentulism through immediate-loaded implant protocolsCLINICAL ORAL IMPLANTS RESEARCH, Issue 1 2008Liene Molly Abstract Objectives: The objective of the present study was to evaluate the influence of immediate loading of implants on speech adaptation. Material and methods: Ten patients (mean age 54, 6 females) were examined before surgery and 1, 3, 6 and 12 months afterwards. Articulation analysis was done using objective DAT-recoded data evaluated by two groups of speech and language therapists and a computer software program. Besides, patient VAS-scores, myofunctional problems and hearing impairment were recorded and analysed. Results: In the present study only one patient suffered from deteriorated speech after immediate loading. Other patients showed unaffected or improved articulation 3 to 6 months after surgery with a strident and interdental pronunciation mostly becoming addental. Furthermore, myofunctional problems occurred in one patient, other patients adapted to the new situation after three months. Hearing impairment did not influence speech pathology in this study. Conclusion: Immediate loading of oral implants does not seem to compromise the normal 3,6 months speech adaptation period. Whether such procedure presents advantages to the conventional 2-stage rehabilitation remains to be investigated. [source] | |||||||||||||