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Counting Procedure (counting + procedure)
Selected AbstractsA reliability-based data treatment system for actual load historyFATIGUE & FRACTURE OF ENGINEERING MATERIALS AND STRUCTURES, Issue 10 2005J. J. XIONG ABSTRACT This paper seeks to establish an integrated and practical data treatment system for actual load history reliability analysis. A convergence,divergence counting procedure is presented to extract all load cycles from a load history of divergence,convergence waves. The lowest number of load history sampling is established on the basis of the damage-based prediction criterion. A parameter estimation formula is proposed for hypothesis testing of the load distribution. The examples of its application for the data treatment of actual load history are given. The proposed data treatment system has been shown to have valid and practical characteristics in analysing reliability results. [source] Mitotic counting in surgical pathology: sampling bias, heterogeneity and statistical uncertaintyHISTOPATHOLOGY, Issue 1 2001F B J M Thunnissen Mitotic counting in surgical pathology: sampling bias, heterogeneity and statistical uncertainty Although several articles on the methodological aspects of mitotic counting have been published, the effects of macroscopic sampling and tumour heterogeneity have not been discussed in any detail. In this review the essential elements for a standardized mitotic counting protocol are described, including microscopic calibration, specific morphological criteria, macroscopic selection, counting procedure, effect of biological variation, threshold, and the setting of an area of uncertainty (,grey area'). We propose that the use of a standard area for mitotic quantification and of a grey area in mitotic counting protocols will facilitate the application of mitotic counting in diagnostic and prognostic pathology. [source] Orthodontic movement in bone defects augmented with Bio-Oss®JOURNAL OF CLINICAL PERIODONTOLOGY, Issue 1 2001An experimental study in dogs Abstract Objective: To study if it was possible to move, by orthodontic means, a tooth into an area of the jaw that had been augmented with Bio-Oss®. Material and Methods: 5 beagle dogs were used. The 1st, 2nd, and 4th mandibular premolars on each side were removed. The defect at the left 4th premolar site was filled with a biomaterial (Bio-Oss®) while the corresponding defect in the right side was left for spontaneous healing. 3 months later, an orthodontic device was inserted in each side of the mandible. The device was designed to allow distal, bodily movement of the 3rd premolars. When the experimental teeth had been moved into the extraction sites of the 4th premolars, the animals were sacrificed and biopsies of the premolar-molar regions of the mandible sampled. The tissues were prepared for histological analysis using standard procedures. In the sections, 3 zones were identified: zone A=the bone tissue within the distal portion of the previous extraction site (4th premolar), zone B=the pressure side of the 3rd premolar, zone C=the tension side of the 3rd premolar. The area occupied by mineralized bone, Bio-Oss® particles and bone marrow was determined by a point counting procedure. The width of the periodontal ligament as well as the percentage of the root surface (in zone B) that exhibited resorption was determined. Results: The findings demonstrated that it was possible to move a tooth into an area of an alveolar ridge that 3 months previously had been augmented with a biomaterial. It was also demonstrated that 12 months after grafting, Bio-Oss® particles remained as inactive filler material in the not utilized part of zone A. The biomaterial was not present in zone C but present in small amounts in zone B. Conclusion: During the orthodontic tooth movement the graft material (Bio-Oss®) was degraded and eliminated from the part of the alveolar ridge that was utilized for the experiment. In the non-utilized part of the ridge the biomaterial, however, remained as a seemingly inactive filler material. [source] Author inflation leads to a breakdown of Lotka's lawJOURNAL OF THE AMERICAN SOCIETY FOR INFORMATION SCIENCE AND TECHNOLOGY, Issue 8 2001Hildrun Kretschmer It is empirically shown that, even using the normal or total counting procedure, Lotka's law breaks down when articles with a large, i.e., more than hundred, number of authors are included in the bibliography. The explanation of this phenomenon is that the conditions for an application of the basic success-breeds-success model are not fulfilled any more. Studying articles with many authors means dealing with items (the articles) having multiple sources (the authors), hence Egghe's generalized success-breeds-success model, leading to not necessarily decreasing distributions, explains the observed irregularities. [source] Detection of pollen grains in multifocal optical microscopy images of air samplesMICROSCOPY RESEARCH AND TECHNIQUE, Issue 6 2009Sander H. Landsmeer Abstract Pollen is a major cause of allergy and monitoring pollen in the air is relevant for diagnostic purposes, development of pollen forecasts, and for biomedical and biological researches. Since counting airborne pollen is a time-consuming task and requires specialized personnel, an automated pollen counting system is desirable. In this article, we present a method for detecting pollen in multifocal optical microscopy images of air samples collected by a Burkard pollen sampler, as a first step in an automated pollen counting procedure. Both color and shape information was used to discriminate pollen grains from other airborne material in the images, such as fungal spores and dirt. A training set of 44 images from successive focal planes (stacks) was used to train the system in recognizing pollen color and for optimization. The performance of the system has been evaluated using a separate set of 17 image stacks containing 65 pollen grains, of which 86% was detected. The obtained precision of 61% can still be increased in the next step of classifying the different pollen in such a counting system. These results show that the detection of pollen is feasible in images from a pollen sampler collecting ambient air. This first step in automated pollen detection may form a reliable basis for an automated pollen counting system. Microsc. Res. Tech., 2009. © 2009 Wiley-Liss, Inc. [source] Fluorescence resonance energy transfer and anisotropy reveals both hetero- and homo-energy transfer in the pleckstrin homology-domain and the parathyroid hormone-receptorMICROSCOPY RESEARCH AND TECHNIQUE, Issue 1 2009Ralf Steinmeyer Abstract We present a method and an apparatus of polarized fluorescence resonance energy transfer (FRET) and anisotropy imaging microscopy done in parallel for improved interpretation of the photophysical interactions. We demonstrate this apparatus to better determine the protein,protein interactions in the pleckstrin homology domain and the conformational changes in the Parathyroid Hormone Receptor, a G-protein coupled receptor, both fused to the cyan and yellow fluorescent proteins for either inter- or intramolecular FRET. In both cases, the expression levels of proteins and also background autofluorescence played a significant role in the depolarization values measured in association with FRET. The system has the sensitivity and low-noise capability of single-fluorophore detection. Using counting procedures from single-molecule methods, control experiments were performed to determine number densities of green fluorescence protein variants CFP and YFP where homo resonance energy transfer can occur. Depolarization values were also determined for flavins, a common molecule of cellular background autofluorescence. From the anisotropy measurements of donor and acceptor, the latter when directly excited or when excited by energy transfer, we find that our instrumentation and method also characterizes crucial effects from homotransfer, polarization specific photobleaching and background molecules. Microsc. Res. Tech., 2009. © 2008 Wiley-Liss, Inc. [source] | ||||||||||