Home About us Contact
|
Home About us Contact | ||
|
|
||
Correct Expression (correct + expression)
Selected AbstractsOn the cyclic crack-tip opening displacementFATIGUE & FRACTURE OF ENGINEERING MATERIALS AND STRUCTURES, Issue 3 2009A. J. McEVILY ABSTRACT This note discusses the cyclic crack tip opening displacement (CTOD)cyc. As is discussed, the correct expression for the (CTOD)cyc is based upon the effective range of the stress intensity factor. [source] Structure of the Mouse Glutamate Decarboxylase 65 Gene and Its PromoterJOURNAL OF NEUROCHEMISTRY, Issue 4 2000Preferential Expression of Its Promoter in the GABAergic Neurons of Transgenic Mice Abstract: GABA is synthesized by glutamate decarboxylase (GAD), which has two forms, GAD65 and GAD67. To elucidate the molecular mechanisms of mouse GAD65 (mGAD65) gene expression, we isolated and characterized the mGAD65 gene. The mGAD65 gene was found to be divided into 16 exons and spread over 75 kb. The sequence of the first exon and the 5,-flanking region indicated the presence of potential neuron-specific cis -regulatory elements. We used transgenic mice to examine the expression pattern conferred by a 9.2-kb promoter-proximal DNA fragment of the mGAD65 gene fused to the bacterial lacZ reporter gene. Transgenic mice showed high ,-galactosidase activity specifically in brain and testis. They also showed characteristic patterns of transgene expression in olfactory bulb, cerebellar cortex, and spinal cord, a similar expression pattern to that of endogenous mGAD65. However, no transgene expression was observed in the ventral thalamus or hypothalamus, in which high mGAD65 gene expression levels have been observed. These results suggest that the 9.2-kb DNA fragment of the mGAD65 gene is associated with its tissue-specific expression and its targeted expression in GABAergic neurons of specific brain regions but that additional regulatory elements are necessary to obtain fully correct expression. [source] Monoclonal Antibodies against the Recombinant Nucleocapsid Protein of Tomato spotted wilt virus and its Application in Virus DetectionJOURNAL OF PHYTOPATHOLOGY, Issue 6 2009Jianxiang Wu Abstract Tomato spotted wilt virus (TSWV) is the type member of the tospovirus genus and causes significant losses in a wide range of economically important ornamental and vegetable crops worldwide. The nucleocapsid gene, located on the ambisense S RNA segment of TSWV was expressed in Escherichia coli using pET-32a as vector and correct expression of recombinant protein was confirmed by Western blot using an anti-TSWV monoclonal antibody (MAb). The recombinant protein was purified using Ni-NTA agarose and the purified protein was used for the production of MAbs. Three murine MAbs against the recombinant nucleocapsid protein were produced. Triple antibody sandwich enzyme-linked immunosorbent assay and immunocapture RT-PCR methods were then established for reliable and efficient detection of TSWV using the produced MAbs. [source] STRATEGIC DEBT AND RJV COMPETITION: ERRATUMAUSTRALIAN ECONOMIC PAPERS, Issue 3 2007SHIOU SHIEH The purpose of this note is to point out that there is a computing error made in the derivation of the t value in Chen (2005). The erratum presents the correct expression of t and the ensuing changes in the results of Chen (2005). [source] | ||||||||||