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Considerable Utility (considerable + utility)
Selected AbstractsB-type natriuretic peptide in the diagnosis of heart failure in the emergency departmentEMERGENCY MEDICINE AUSTRALASIA, Issue 2 2006Robert Chircop Abstract B-type natriuretic peptide (BNP) is increasingly being used as a diagnostic marker in the diagnosis of heart failure. Here we evaluate the evidence base for its utility in the ED. Clinical trials suggest that it is more accurate than clinical acumen especially when emergency physicians have diagnostic problems. BNP appears more accurate than any clinical findings or radiological signs. In conjunction with considered clinical judgement, knowledge of its limitations and variable cut-off points, BNP can be of considerable utility to the emergency physician. [source] New Directions for IPE: Drawing From Behavioral EconomicsINTERNATIONAL STUDIES REVIEW, Issue 2 2008Deborah Kay Elms Many of the research approaches currently under investigation by behavioral economists are even better suited for international political economy research. The three research ideas illustrated in this article,framing and loss aversion, myopic time horizons, and fairness,highlight concepts of considerable utility for IPE researchers. This article uses previously published International Organization articles, reformulated to consider the same puzzles from a different angle, to illustrate the application of these concepts. This allows readers the opportunity to consider an extended comparison of theoretical explanations of the same empirical evidence. Incorporating more of the knowledge drawn from psychology and current economics will yield superior explanations for political economy research questions that are more accurate, generalizable, parsimonious, and testable. [source] An intricate network of regulators controls biofilm formation and colonization by Vibrio fischeriMOLECULAR MICROBIOLOGY, Issue 4 2009Karen L. Visick Summary The initial encounter between a microbe and its host can dictate the success of the interaction, be it symbiosis or pathogenesis. This is the case, for example, in the symbiosis between the bacterium Vibrio fischeri and the squid Euprymna scolopes, which proceeds via a biofilm-like bacterial aggregation, followed by entry and growth. A key regulator, the sensor kinase RscS, is critical for symbiotic biofilm formation and colonization. When introduced into a fish symbiont strain that naturally lacks the rscS gene and cannot colonize squid, RscS permits colonization, thereby extending the host range of these bacteria. RscS controls biofilm formation by inducing transcription of the symbiosis polysaccharide (syp) gene locus. Transcription of syp also requires the ,54 -dependent activator SypG, which functions downstream of RscS. In addition to these regulators, SypE, a response regulator that lacks an apparent DNA binding domain, exerts both positive and negative control over biofilm formation. The putative sensor kinase SypF and the putative response regulator VpsR, both of which contribute to control of cellulose production, also influence biofilm formation. The wealth of regulators and the correlation between biofilm formation and colonization adds to the already considerable utility of the V. fischeri,E. scolopes model system. [source] Generation of a triple-gene knockout mammalian cell line using engineered zinc-finger nucleases,BIOTECHNOLOGY & BIOENGINEERING, Issue 1 2010Pei-Qi Liu Abstract Mammalian cells with multi-gene knockouts could be of considerable utility in research, drug discovery, and cell-based therapeutics. However, existing methods for targeted gene deletion require sequential rounds of homologous recombination and drug selection to isolate rare desired events,a process sufficiently laborious to limit application to individual loci. Here we present a solution to this problem. Firstly, we report the development of zinc-finger nucleases (ZFNs) targeted to cleave three independent genes with known null phenotypes. Mammalian cells exposed to each ZFN pair in turn resulted in the generation of cell lines harboring single, double, and triple gene knockouts, that is, the successful disruption of two, four, and six alleles. All three biallelic knockout events were obtained at frequencies of >1% without the use of selection, displayed the expected knockout phenotype(s), and harbored DNA mutations centered at the ZFN binding sites. These data demonstrate the utility of ZFNs in multi-locus genome engineering. Biotechnol. Bioeng. 2010; 106: 97,105. © 2009 Wiley Periodicals, Inc. [source] | ||||||||||