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Terms modified by Consequent Selected AbstractsPre-rainy season moisture build-up and storm precipitation delivery in the West African SahelINTERNATIONAL JOURNAL OF CLIMATOLOGY, Issue 7 2008J. Bayo Omotosho Abstract The salient differences between the years of above and below normal precipitation, particularly within the long period of 1972,1990 with persistently decreasing Sahelian rainfall, are investigated for Kano, a Nigerian station within the Sahel. Daily rainfall data from 1916 to 2000, storm records from 1951 to 2000 and radiosonde data for three dry and three wet years are used in this study. Results confirm previous findings that the African Easterly Jet (AEJ) located in the 700,600 mb layer is stronger during the dry than in wet years. Significantly, however, it is shown that during the wet years, there is stronger and deeper early season (April,June) build-up of moisture below the AEJ. Furthermore, throughout the period from April to August, the middle troposphere was almost always drier than normal during the dry years and moist than normal in the wet years. Consequent upon these, the storms, which deliver almost all the rainfall in the Sahel, produce at least 150% more precipitation during the wet than in the dry years, though the June to September or annual total number of storms differs by only about 30%. Finally, during the dry years, the onset of rainfall is found to be generally very late compared to the long-term mean, with shorter length of the rainy season. Copyright © 2007 Royal Meteorological Society [source] Tuberculosis verrucosa cutis: antitubercular therapy, a well-conceived diagnostic criterionINTERNATIONAL JOURNAL OF DERMATOLOGY, Issue 3 2005Virendra N. Sehgal MD A 39-year-old housewife sustained inadvertent trauma to the right index finger about 6 years ago, whilst stitching clothes. A couple of weeks later, the site of trauma became hard and gritty. Ever since, it has progressed slowly, without any appreciable outward sign. It was not associated with any discomfort/pain. Consequent on an opinion from a surgeon, it was decided to operate on the right index finger. During the operation, under local anesthesia, a hard and gritty material was removed. The material was subjected to histopathologic study. Several stitches were applied to the wound. It failed to respond to antimicrobial therapy over a 4-week period, prompting the patient to seek another opinion. Examination of the skin surface revealed a plaque with an irregular configuration on and around the distal interphalangeal joint of the right index finger. It was erythematous and pigmented. The top of the plaque was irregular and had alternating elevations and depressions (Fig. 1). Diascopy was negative for apple jelly nodule. A bacillus Calmette,Guérin (BCG) vaccination scar was identified on the left deltoid. There was no regional lymphadenopathy or systemic abnormality. Mantoux test with intradermal injection of 0.1 mL SPAN's tuberculin (purified protein derivative/5 tuberculin units/0.1 mL) (Span Diagnostic Ltd., Murat, India) was negative after 72 h. Investigations, including total and differential leukocyte count, erythrocyte sedimentation rate, serum biochemistry, and renal and liver function tests, were within the normal range, as was a chest X-ray. Figure 1. Tuberculosis verrucosa cutis before (a) and after (b) antitubercular therapy (ATT) Hematoxylin and eosin-stained sections prepared from the biopsy taken from the lesion revealed noteworthy changes in the epidermis and the dermis. The former was marked by the presence of hyperkeratosis, acanthosis, and papillomatosis, whilst the latter contained tubercle granulomas. Each of the granulomas was well formed and consisted of large numbers of lymphocytes, histiocytes, and foreign body (Langerhans') giant cells (Fig. 2). Caseation necrosis and acid-fast bacilli could not be demonstrated. The preceding revelations were fairly conducive to the diagnosis. Accordingly, antitubercular therapy (ATT), comprising 450 mg of rifampicin, 300 mg of isonicotinic acid hydrazide, and 800 mg of ethambutol, was recommended for oral administration each day for 60 days. The outcome of the treatment was satisfactory, resulting in perceptible regression of the skin lesion (Fig. 1b). The patient was advised to continue the treatment for another 30 days, after which 450 mg of rifampicin and 300 mg of isonicotinic acid hydrazide were to be continued for another 6 months. Figure 2. Tuberculosis verrucosa cutis depicting well-formed tubercle(s) comprising lymphocytes, histiocytes, neutrophils, and a few giant cells (hematoxylin and eosin, × 100) [source] Ontogeny of tyrosine hydroxylase mRNA expression in mid- and forebrain: Neuromeric pattern and novel positive regionsDEVELOPMENTAL DYNAMICS, Issue 3 2005Faustino Marín Abstract Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of catecholamines and, thus, critical in determining the catecholaminergic phenotype. In this study, we have examined the expression of TH mRNA by in situ hybridization in the embryonic mouse forebrain and midbrain and have mapped its localization according to the neuromeric pattern. We find that early in embryonic development, 10 to 12 days post coitum (dpc), TH mRNA is expressed in ample continuous regions of the neuroepithelium, extending across several neuromeres. However, from 12.5 dpc onward, the expression becomes restricted to discrete regions, which correspond to the dopaminergic nuclei (A8 to A15). In addition to these nuclei previously described, TH mRNA is also observed in regions that do not express this enzyme according to immunohistochemical studies. This difference in relation to protein expression pattern is consequent with the known posttranscriptional regulation of TH expression. The most representative example of a novel positive region is the conspicuous mRNA expression in both medial and lateral ganglionic eminences. This result agrees with reports describing the capacity of striatal stem cells (that is, located at the lateral ganglionic eminence) to become dopaminergic in vitro. Other regions include the isthmic mantle layer and the early floor plate of the midbrain,caudal forebrain. On the whole, the expression map we have obtained opens new perspectives for evolutionary/comparative studies, as well as for therapeutic approaches looking for potentially dopaminergic cells. Developmental Dynamics 234:709,717, 2005. © 2005 Wiley-Liss, Inc. [source] Differing aspects of insulin resistance in diabetes complications: the shape of things to come.DIABETIC MEDICINE, Issue 12 2002RD Lawrence lecture 2000 Abstract The anticipated global epidemic of diabetes, largely as a consequence of increased rates of obesity, will particularly impact on people of South Asian and African Caribbean descent, two populations at elevated risk of insulin resistance. This article contrasts the consequent heightened risk of heart disease on the one hand in South Asians, and the paradoxical protection from heart disease in African Caribbeans on the other. Protection from the hypertriglyceridaemic effects of insulin resistance is likely to account for much of the African Caribbean paradox, although the mechanisms remain unclear. The growing evidence that insulin resistance is commonly observed in people with Type 1 diabetes, as well as those with Type 2 diabetes, and that features of insulin resistance may play a crucial role in the development of microvascular, as well as macrovascular complications, is also discussed. This indicates novel targets for the prevention and treatment of diabetes complications. Diabet. Med. 19, 973,977 (2002) [source] Functional Interpretations of Constructive Set Theory in All Finite TypesDIALECTICA, Issue 2 2008Justus Diller Gödel's dialectica interpretation of Heyting arithmetic HA may be seen as expressing a lack of confidence in our understanding of unbounded quantification. Instead of formally proving an implication with an existential consequent or with a universal antecedent, the dialectica interpretation asks, under suitable conditions, for explicit ,interpreting' instances that make the implication valid. For proofs in constructive set theory CZF - , it may not always be possible to find just one such instance, but it must suffice to explicitly name a set consisting of such interpreting instances. The aim of eliminating unbounded quantification in favor of appropriate constructive functionals will still be obtained, as our ,-interpretation theorem for constructive set theory in all finite types CZF, - shows. By changing to a hybrid interpretation ,q, we show closure of CZF, - under rules that , in stronger forms , have already been studied in the context of Heyting arithmetic. In a similar spirit, we briefly survey modified realizability of CZF, - and its hybrids. Central results of this paper have been proved by Burr 2000a and Schulte 2006, however, for different translations. We use a simplified interpretation that goes back to Diller and Nahm 1974. A novel element is a lemma on absorption of bounds which is essential for the smooth operation of our translation. [source] Elongation Index as a New Index Determining the Severity of Left Ventricular Systolic Dysfunction and Mitral Regurgitation in Patients with Congestive Heart FailureECHOCARDIOGRAPHY, Issue 7 2005Mehmet Yokusoglu M.D. The shape of the left ventricle is an important echocardiographic feature of left ventricular dysfunction. Progression of the mitral regurgitation and consequent left ventricular remodeling is unpredictable in heart failure. Elongation index is an index of left ventricular sphericity. The surface area of the elongated ventricle is larger than that of a spherical one. The objective of this study was to assess the relation between elongation index and the degree of mitral regurgitation along with noninvasive indices of left ventricular function. Thirty-two patients (21 male, 11 female, mean age: 57 ± 6 yrs) with congestive heart failure and mitral regurgitation were included. Patients were stratified into three groups according to vena contracta width as having mild (n = 11), moderate (n = 11) and severe mitral regurgitation (n = 10). The elongation index (EI) was considered as equal to {[(left ventricular internal area-measured) , (theoretical area of the sphere with measured left ventricular volume)]/(theoretical area of the sphere with measured left ventricular volume)}. Ejection fractions by the modified Simpson rule, dP/dt and sphericity index (SI) were also recorded. The relationship between (EI), ejection fraction, dP/dt and SI reached modest statistical significance (p < 0.05). When the EI and SI were compared, the correlation was also significant (p < 0.01). The areas under the receiver operator curve of EI and SI for discriminating dP/dt < 1000 mm Hg/s were 0.833 and 0.733, respectively. In conclusion, the elongation, which defines the shape of the left ventricle, might be related to the systolic function of the left ventricle and the degree of the mitral regurgitation. Further studies are needed to demonstrate its use in other clinical entities. [source] Mass regulation in response to predation risk can indicate population declinesECOLOGY LETTERS, Issue 10 2007Ross MacLeod Abstract In theory, survival rates and consequent population status might be predictable from instantaneous behavioural measures of how animals prioritize foraging vs. avoiding predation. We show, for the 30 most common small bird species ringed in the UK, that one quarter respond to higher predation risk as if it is mass-dependent and lose mass. Half respond to predation risk as if it only interrupts their foraging and gain mass thus avoiding consequent increased starvation risk from reduced foraging time. These mass responses to higher predation risk are correlated with population and conservation status both within and between species (and independently of foraging habitat, foraging guild, sociality index and size) over the last 30 years in Britain, with mass loss being associated with declining populations and mass gain with increasing populations. If individuals show an interrupted foraging response to higher predation risk, they are likely to be experiencing a high quality foraging environment that should lead to higher survival. Whereas individuals that show a mass-dependent foraging response are likely to be in lower quality foraging environments, leading to relatively lower survival. [source] Onco-miR-155 targets SHIP1 to promote TNF,-dependent growth of B cell lymphomasEMBO MOLECULAR MEDICINE, Issue 5 2009Irene M. Pedersen Abstract Non-coding microRNAs (miRs) are a vital component of post-transcriptional modulation of protein expression and, like coding mRNAs harbour oncogenic properties. However, the mechanisms governing miR expression and the identity of the affected transcripts remain poorly understood. Here we identify the inositol phosphatase SHIP1 as a bonafide target of the oncogenic miR-155. We demonstrate that in diffuse large B cell lymphoma (DLBCL) elevated levels of miR-155, and consequent diminished SHIP1 expression are the result of autocrine stimulation by the pro-inflammatory cytokine tumour necrosis factor , (TNF,). Anti-TNF, regimen such as eternacept or infliximab were sufficient to reduce miR-155 levels and restored SHIP1 expression in DLBCL cells with an accompanying reduction in cell proliferation. Furthermore, we observed a substantial decrease in tumour burden in DLBCL xenografts in response to eternacept. These findings strongly support the concept that cytokine-regulated miRs can function as a crucial link between inflammation and cancer, and illustrate the feasibility of anti-TNF, therapy as a novel and immediately accessible (co)treatment for DLBCL. [source] Craving: what can be done to bring the insights of neuroscience, behavioral science and clinical science into synchronyADDICTION, Issue 8s2 2000Roger E. Meyer Alcohol self-administration behavior is the common thread that is necessary to bring the insights of neuroscience, behavioral science and clinical science into synchrony around the concept of craving. Animal models should address the molecular and cellular changes that take place in behaviorally relevant brain regions of rats consequent to chronic self-administration of ethanol. Animal models can focus on the biology of the anticipatory state in alcohol preferring/consuming rats, as well as studies of the effects of possible medications on this state in the animal model, on actual alcohol consuming behavior, and on the residual effects of chronic alcohol on the non-human mammalian brain. In human studies of craving, cue-reactivity in the absence of the opportunity to drink alcohol does not have the same salience as cue-reactivity in which drinking is possible. Moreover, actual drinking behavior serves to validate self-reports of craving. Studies of limited alcohol self-administration in the laboratory are an essential element in screening new medications for the treatment of alcoholism. Studies to date suggest no adverse reaction to the participation of alcoholic subjects in limited alcohol self-administration studies, but the research community should continue to monitor carefully the outcomes of alcohol-dependent subjects who participate in this type of research, and efforts should always be made to encourage these subjects to enter active treatment. In outpatient clinical trials of new treatments for alcoholism, the assessment of craving should include queries regarding symptoms and signs of protracted abstinence such as sleep disturbances, as well as questions regarding situational craving. Field observations of alcoholics in their favorite drinking environments would contribute greatly to our understanding of the real-world phenomenology of craving. [source] Revascularization in acute ischaemic stroke using the penumbra system: the first single center experienceEUROPEAN JOURNAL OF NEUROLOGY, Issue 11 2009I. Q. Grunwald Background and purpose:, This is the first single center experience illustrating the effectiveness of the penumbra system (PS) in the treatment of large vessel occlusive disease in the arena of acute ischaemic stroke. The PS is an innovative mechanical thrombectomy device, employed in the revascularization of large cerebral vessel occlusions in patients via the utilization of an aspiration platform. Methods:, This is a prospective, non-randomized controlled trial evaluating the clinical and functional outcome in 29 patients with acute intra-cranial occlusions consequent to mechanical thrombectomy by the PS either as mono-therapy or as an adjunct to current standard of care. Patients were evaluated by a neurologist and treated by our in house interventional neuro-radiologists. Primary end-points were revascularization of the occluded target vessel to TIMI grade 2 or 3 and neurological outcome as measured by an improvement in the NIH Stroke Scale (NIHSS) score after the procedure. Results:, Complete revascularization (TIMI 3) was achieved in 21/29 (72.4%) of patients. Partial revascularization (TIMI 2) was established in 4/29 (13.8%) of patients. Revascularization failed in four (13.8%) patients. Nineteen (19) patients (65.5%) had at least a four-point improvement in NIHSS scores. Modified Rankin scale scores of ,2 were seen in 37.9% of patients. There were no device-related adverse events. Symptomatic intra-cranial hemorrhage occurred in 7% of patients. Conclusions:, The PS has the potential of exercising a significant impact in the interventional treatment of ischaemic stroke in the future. [source] Associative Pavlovian conditioning leads to an increase in spinophilin-immunoreactive dendritic spines in the lateral amygdalaEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 3 2006Jason J. Radley Abstract Changes in dendritic spine number and shape are believed to reflect structural plasticity consequent to learning. Previous studies have strongly suggested that the dorsal subnucleus of the lateral amygdala is an important site of physiological plasticity in Pavlovian fear conditioning. In the present study, we examined the effect of auditory fear conditioning on dendritic spine numbers in the dorsal subnucleus of the lateral amygdala using an immunolabelling procedure to visualize the spine-associated protein spinophilin. Associatively conditioned rats that received paired tone and shock presentations had 35% more total spinophilin-immunoreactive spines than animals that had unpaired stimulation, consistent with the idea that changes in the number of dendritic spines occur during learning and account in part for memory. [source] Deploying the Classic ,Community Method' in the Social Policy Field: The Example of the Acquired Rights DirectiveEUROPEAN LAW JOURNAL, Issue 2 2009Gavin Barrett The use of the Community method of legislation, in particular the deployment of directives, has for a long time been at the core of EC labour market policy. This article seeks to reflect on the lessons to be learned from the experience of the adoption and operation of one particularly significant directive, namely the Acquired Rights Directive, and on the experience of its transposition in one Member State, Ireland. Among features noted at the EU level are the watering down of the Commission's initial legislative ambitions; the substantial lacunae, failures to address issues and ambiguities incorporated in the text of the directive, the consequent enlarged role for the Court of Justice and the apparent difficulty in changing policy direction in the event of errors being made. As regards the Irish experience of transposing the directive, lessons learnt have included the importance of the means of implementation chosen by the Member State; the obstructive effect which national industrial relations systems may have on the evolution of a common European approach; the significance which attaches to national sanctions and enforcement mechanisms; the importance attaching to the degree of collective organisation in workplaces where the implementing legislation is sought to be relied upon; and the potential which the implementation of a directive has for disruption of the harmony of a national policy approach. Finally, the use of a form of social dialogue in the implementation of employment-related directives in Ireland is also commented upon. [source] Calcium and Fos Involvement in Brain-Derived Ca2+ -Binding Protein (S100)-Dependent Apoptosis in Rat Phaeochromocytoma CellsEXPERIMENTAL PHYSIOLOGY, Issue 3 2000Stefania Fulle Brain-derived calcium-binding protein S100 induces apoptosis in a significant fraction of rat phaeochromocytoma (PC12) cells. We used single cell techniques (patch clamp, videomicroscopy and immunocytochemistry) to clarify some of the specific aspects of S100-induced apoptosis, the modality(ies) of early intracellular Ca2+ concentration increase and the expression of some classes of genes (c-fos, c-jun, bax, bcl-x, p-15, p-21) known to be implicated in apoptosis of different cells. The results show that S100: (1) causes an increase of [Ca2+]i due to an increased conductance of L-type Ca2+ channels; (2) induces a sustained increase of the Fos levels which is evident since the first time point tested (3 h) and remains elevated until to the last time point (72 h). All these data suggest that S100-derived apoptosis in PC12 cells may be the consequence of a system involving an increase in L-type Ca2+ channel conductance with consequent [Ca2+]i increase which up-regulates, directly or indirectly, the expression of Fos. [source] Thermosynechoccus elongatus DpsA binds Zn(II) at a unique three histidine-containing ferroxidase center and utilizes O2 as iron oxidant with very high efficiency, unlike the typical Dps proteinsFEBS JOURNAL, Issue 4 2010Flaminia Alaleona The cyanobacterium Thermosynechococcus elongatus is one the few bacteria to possess two Dps proteins, DpsA-Te and Dps-Te. The present characterization of DpsA-Te reveals unusual structural and functional features that differentiate it from Dps-Te and the other known Dps proteins. Notably, two Zn(II) are bound at the ferroxidase center, owing to the unique substitution of a metal ligand at the A-site (His78 in place of the canonical aspartate) and to the presence of a histidine (His164) in place of a hydrophobic residue at a metal-coordinating distance in the B-site. Only the latter Zn(II) is displaced by incoming iron, such that Zn(II),Fe(III) complexes are formed upon oxidation, as indicated by absorbance and atomic emission spectroscopy data. In contrast to the typical behavior of Dps proteins, where Fe(II) oxidation by H2O2 is about 100-fold faster than by O2, in DpsA-Te the ferroxidation efficiency of O2 is very high and resembles that of H2O2. Oxygraphic experiments show that two Fe(II) are required to reduce O2, and that H2O2 is not released into solution at the end of the reaction. On this basis, a reaction mechanism is proposed that also takes into account the formation of Zn(II),Fe(III) complexes. The physiological significance of the DpsA-Te behavior is discussed in the framework of a possible localization of the protein at the thylakoid membranes, where photosynthesis takes place, with the consequent increased formation of reactive oxygen species. Structured digital abstract ,,MINT-7312099: DpsA (uniprotkb:Q8DL82) and DpsA (uniprotkb:Q8DL82) bind (MI:0407) by x-ray crystallography (MI:0114) [source] Productive Chlamydia trachomatis lymphogranuloma venereum 434 infection in cells with augmented or inactivated autophagic activitiesFEMS MICROBIOLOGY LETTERS, Issue 2 2009Niseema Pachikara Abstract Autophagy, a eukaryotic cellular activity leading to the degradation of cellular components, serves as a defense mechanism against facultative intracellular bacteria as well as a growth niche for the obligate intracellular bacterium Coxiella burnetii. We here demonstrate that the obligate intracellular bacterial pathogen Chlamydia trachomatis lymphogranuloma venereum strongly induced autophagy in the middle of the chlamydial developmental cycle (24 h after infection), a time point with maximal level of chlamydial replication, but not during the early stages with low overall chlamydial metabolism (before 8 h). No autophagy induction was evident in cells exposed to heat- and UV-inactivated elementary bodies (EBs, the infectious form of Chlamydia) or to inocula from which EBs had been removed before inoculation. Blocking chlamydial development with chloramphenicol also prevented autophagy induction in cells infected with infectious EBs. It appears that autophagy is activated primarily in response to the metabolic stress consequent to chlamydial replication. However, autophagy-defective ATG5,/, cells supported chlamydial development as efficiently as autophagy-proficient ATG5+/+ cells. [source] Performing Women: The Gendered Dimensions of the UK New Research EconomyGENDER, WORK & ORGANISATION, Issue 5 2007Catherine Fletcher This article explores the development and maintenance of familiar gendered employment patterns and practices in UK universities, which are exemplars of new modes of knowledge production, commodification and marketization. After discussing in detail the evidence of gender discrimination in UK higher education and the changes in the academic labour process consequent to the incorporation of universities, at least at the policy level, into the ,knowledge economy', institution-specific data is used to highlight the gendered aspects of the research economy from the three intermeshing perspectives of research culture, research capital and the research production process. This nexus is constructed in such a way as to systematically militate against women's full and equal involvement in research. Lack of transparency, increased competition and lower levels of collegiate activity coupled with networking based on homosociability are contributing to a research production process where women are marginalized. [source] Ancient transpacific voyaging to the new world via Pleistocene South Pacific IslandsGEOARCHAEOLOGY: AN INTERNATIONAL JOURNAL, Issue 6 2004Steve Wyatt How humans first arrived in America remains a mystery. Although the Beringian and coastal options have been discussed in detail, a transpacific route from the Old World to the New World via the islands of Oceania has been essentially ignored. Of the many factors involved in completing such a voyage, besides an adequate watercraft, landfall frequency and prevailing winds and currents were most important. A chain of islands in the landless eastern South Pacific, with its consequent and possibly favorable modifications of regional sea surface currents, would have been particularly beneficial to eastbound mariners. Comparing present-day bathymetry with estimated late Pleistocene glacially induced sea level fluctuations suggests that latent islands may actually exist, especially when the effects of other geological phenomena are also considered. If exposed during the last glacial maximum (LGM), such a chain of islands could have provided facilitating layover points for ancient eastbound seafaring explorers, thus making a transpacific journey more plausible. © 2004 Wiley Periodicals, Inc. [source] The response of manured forage maize to starter phosphorus fertilizer on chalkland soils in southern EnglandGRASS & FORAGE SCIENCE, Issue 2 2000Withers The impact of various starter phosphorus (P) fertilizers on the growth, nutrient uptake and dry-matter (DM) yield of forage maize (Zea mais) continuously cropped on the same area and receiving annual, pre-sowing, broadcast dressings of liquid and semi-solid dairy manures was investigated in two replicated plot experiments and in whole-field comparisons in the UK. In Experiment 1 on a shallow calcareous soil (27 mg l,1 Olsen-extractable P) in 1996, placement of starter P fertilizer (17 or 32 kg ha,1) did not benefit crop growth or significantly (P > 0·05) increase DM yield at harvest. However, in Experiment 2 on a deeper non-calcareous soil (41 mg l,1 Olsen-extractable P) in 1997, placement of starter P fertilizer (19 or 41 kg P ha,1), either applied alone or in combination with starter N fertilizer (10 or 25 kg N ha,1), significantly increased early crop growth (P < 0·01) and DM yield at harvest by 1·3 t ha,1 (P < 0·05) compared with a control without starter N or P fertilizer. Placement of starter N fertilizer alone did not benefit early crop growth, but gave similar yields as P, or N and P, fertilizer treatments at harvest. Large treatment differences in N and P uptake by mid-August had disappeared by harvest. In field comparisons over the 4-year period 1994,97, the addition of starter P fertilizer increased field cumulative surplus P by over 70%, but without significantly (P > 0·05) increasing DM yield, or nutrient (N and P) uptake, compared with fields that did not receive starter P fertilizer. The results emphasized the extremely low efficiency with which starter P fertilizers are utilized by forage maize and the need to budget manure and fertilizer P inputs more precisely in order to avoid excessive soil P accumulation and the consequent increased risk of P transfer to water causing eutrophication. [source] Domiciliary application of CryoCuff in severe haemophilia: qualitative questionnaire and clinical auditHAEMOPHILIA, Issue 4 2008A. I. D'YOUNG Abstract., The acute management of haemophilic bleeding episodesin the home setting is based on the concept of immediate factor replacement therapy and the PRICE regime , an acronym representing the concepts of Protection, Rest, Ice, Compression and Elevation [1,2]. Integral to this regime is the application of cold therapy, and yet little is known regarding the safe periods of application, or the relative safety of cryotherapy devices such as the CryoCuffÔ when used in the home setting by patients suffering from severe haemophilia and related bleeding disorders. This study examines the subjective patient response to the application of the CryoCuffÔ device in the home setting in terms of the effect on pain, joint swelling and the return to ,pre-bleed status' of the knee, ankle or elbow in patients with severe haemophilia A/B or type III von Willebrand's disease (VWD) immediately following haemarthrosis, and any potential adverse effects related to the device or recommended duration of application as stated in the PRICE guideline (Fig. 1). Twelve patients, either with severe haemophilia A/B or with VWD were recruited and asked to use the CryoCuffÔ device as part of the PRICE regime immediately following the onset of knee-, ankle- or elbow bleeds for the next one year. Each subject was then sent a qualitative questionnaire to determine subjective responses to the device. All patients reported that the application protocol was easy to follow, they were able to apply the device as per the PRICE regime and they were able to tolerate it for the recommended period. Whereas, all the patients felt that the device had a significant impact on alleviation of pain and return to pre-bleed status, 78% of the patients felt that the device led to a significant reduction in swelling around the affected joint. There was no conclusive evidence that the device resulted in any reduction in the amount of factor used to treat the acute bleeding episode, however, no patients reported any perceived delay in achieving haemostasis or required extra factor replacement therapy consequent to the usage of the device. No other adverse effects were reported by participants in this study. Figure 1. ,The qualitative participant questionnaire, given following 1 year of unsupervised use in the home setting immediately following the onset of the symptoms of haemarthroses. [source] Circulating T-Cell Response to Helicobacter pylori Infection in Chronic GastritisHELICOBACTER, Issue 3 2000Zhigang Ren Background.Helicobacter pylori elicits a specific humoral and cellular immune response. There is increasing evidence that the type of T-cell response contributes to clinical outcome in H. pylori infection. Materials and Methods. The host response to H. pylori infection in 34 subjects with chronic gastritis was examined in terms of T-cell proliferation and cytokine production in whole-blood cultures stimulated or unstimulated with H. pylori acid-glycine extract antigens (AGE). Results. The proliferative response in whole-blood cultures was similar for both H. pylori,positive and ,negative subjects stimulated with H. pylori AGE. While an increase in interferon-, (IFN-,) production was observed from both H. pylori,positive and ,negative subjects with gastritis, significantly higher levels of IFN-, were detected in the former when stimulated with H. pylori AGE. In contrast, interleukin 4 (IL-4) was undetectable regardless of antigen stimulation. However, if an in situ IL-4 antibody capture assay was used, antigen-independent production of IL-4 was detected, but there was no difference between H. pylori,positive and ,negative subjects with gastritis. After eradication of H. pylori, antigen-induced production of IL-4 was increased, with no decrease in the levels of secretion of IFN-,. IL-4 production was dependent on CD4+ T cells, as addition of anti-CD4 but not anti-CD8 mouse monoclonal antibody or matched IgG isotype to the whole-blood culture inhibited the production of IL-4. Conclusion. The results suggest that a shift toward a balanced Th1-Th2 response due to an increase in antigen-induced IL-4 production from CD4+ T cells follows eradication. We suggest that the downregulation of mucosal inflammation consequent on reduction in antigen levels or removal of downregulation after eradication of H. pylori contributes to this shift in cytokine balance. [source] Transcription factor NF-,B activation after in vivo perforant path LTP in mouse hippocampusHIPPOCAMPUS, Issue 6 2004Ramiro Freudenthal Abstract There is increasing evidence that transcription factors (TFs) play a critical role in maintaining later phases of hippocampal long-term potentiation (LTP). We have been led to study the role in synaptic plasticity of the powerful, yet generally unheralded, NF-,B TF because it may serve as both a signaling molecule after its activation at the synapse and then a transcription initiator upon reaching the nucleus. In the present study, we show that LTP activates NF-,B in the intact mouse hippocampus. Mice were sacrificed 15 min after one of three treatments: tetanization (high-frequency stimulation [HFS]), low-frequency stimulation (LFS), or no stimulated control animals (CT). In a first study, nuclear NF-,B activity from hippocampus was estimated by electrophoretic mobility shift assays (EMSAs). A higher level of hippocampal TF binding to the NF-,B recognition element was found in the HFS group compared with LFS or CT. In a second study, NF-,B activity was evaluated by immunohistochemistry with a specific antibody that recognizes the activated form of NF-,B. This antibody binds to the exposed nuclear location sequence on the p65 subunit of NF-,B consequent to its dissociation from the inhibitory I,B molecule. In the four subfields of hippocampus examined,granule cell layer, hilus of the dentate gyrus, CA3 and CA1 pyramidal fields of the hippocampal gyrus,the highest levels of activated NF-,B, statistically significant in all cases were found after HFS. In certain comparisons, LFS animals also showed significant elevation with respect to CT. These results support the role of NF-,B as part of the synaptic signaling and transcriptional regulation mechanism required in long-term plasticity, emphasizing the combinatorial nature of TF function. © 2004 Wiley-Liss, Inc. [source] Recharge, upflux and water table response for shallow water table conditions in southwest FloridaHYDROLOGICAL PROCESSES, Issue 9 2006Fouad H. Jaber Abstract A disproportionate increase or decrease in water table in response to minor water input or drainage is observed in shallow water table conditions inside drainage lysimeters. This increase happens because the capillary fringe of the shallow water table reaches up to or near the surface (Wieringermeer effect). The correlations between water table level changes and rainfall, seepage irrigation, drip irrigation, and drainage were analysed. Correlations with rainfall, seepage irrigation, and drainage were high (R2 ranged from 0·46 to 0·97). Drip irrigation had low correlations due to the low rates of application (R2 ranged from 0·26 to 0·44). Conventional methods of calculating recharge, such as multiplying the specific yield with the water table fluctuations, cannot be used for Wieringermeer effect situations. A method using water balance data and soil moisture at different depths in the lysimeters was developed to estimate recharge and upflux. The recharge results were used to develop the apparent specific yield Sya, which could be used to calculate consequent recharge events from water table fluctuation data. Combining the water table fluctuation relationships developed with the Sya value will allow the prediction of recharge from rainfall and irrigation events without the need for soil moisture equipment. Copyright © 2005 John Wiley & Sons, Ltd. [source] Botulinum toxin injection therapy in the management of lower urinary tract dysfunctionINTERNATIONAL JOURNAL OF CLINICAL PRACTICE, Issue 2006A. K. PATEL Summary We have great pleasure in introducing this supplement containing a collection of articles reviewing the contemporary clinical management of functional disorders of the lower urinary tract (LUT) with particular emphasis on the potential role of botulinum toxin injection therapy. Detrusor sphincter dyssynergia (DSD), detrusor overactivity (DO), painful bladder syndrome (PBS) and LUT symptoms consequent on bladder outflow obstruction (LUTS/BPH) have all been treated by the injection of botulinum toxin. This treatment can be administered as a minimally invasive, outpatient procedure which on the initial trials for DO (particularly of neurogenic aetiology) shows a remarkable efficacy with effects lasting up to a year after a single treatment with few significant side effects. Success has been reported with the management of detrusor sphincter dyssynergia and preliminary series report positive outcomes in the management of PBS and LUTS/BPH. However, most of the studies to date include small numbers and have a recruitment bias with few randomised controlled trials having been reported. The answers to some of the key questions are addressed with reference to our contemporary knowledge. It is clear that considerable work both clinical and basic science still needs to be performed to answer the many remaining questions with regard to this treatment modality but undoubtedly it will be a major future treatment option in those with intractable symptoms or those unable to tolerate medications. Currently, all botulinum toxin use for urological conditions is off-label and unlicensed, therefore caution should be exercised until future large randomised studies are reported. [source] Needs assessment in dementiaINTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY, Issue 4 2005A. M. Meaney Abstract Background Resource allocation and service development traditionally focuses on diagnostic categories and consequent perceived need. Identification of the actual level of need in the elderly with dementia, and the degree to which it is unmet is necessary to plan services both individually and as a group. The aim of this study was to characterise the needs of a sample of community dwelling elderly patients with dementia who were referred to an old age psychiatry service in Ireland between July 2002 and July 2003. Methodology Eighty-two consecutively referred community dwelling patients with ICD-10 diagnosis of dementia were assessed on The Care Needs Assessment Pack for Dementia (CareNap-D). Data on needs across seven domains (health and mobility, self-care and toileting, social interaction, thinking and memory, behaviour and mental state, housecare, community living) is presented (Reynolds T et al., 1998). Results Subjects had a mean of 33 (range: 13,56) identified needs. Approximately 1/3 of these were unmet with a mean of 13 (range: 0,37) and a mean of 20 (range: 4,39) were met. High levels of unmet need was identified in the domains of behaviour and mental state (84% of those with agitation) and of social interaction (79% of those with ,partaking in activities' need). The specific item of repetitive questioning occurred in 68 individuals and was unmet in 88% of these cases. Increasing age, lower MMSE score, and living alone were associated with greater total levels of unmet need. Conclusion This data underlines the degree of unmet need in the community dwelling elderly with dementia and the importance of developing a spectrum of services on the basis of the actual needs identified. Copyright © 2005 John Wiley & Sons, Ltd. [source] Emergence of self-learning fuzzy systems by a new virus DNA,based evolutionary algorithmINTERNATIONAL JOURNAL OF INTELLIGENT SYSTEMS, Issue 3 2003Lihong Ren In this article, we propose a new approach to the virus DNA,based evolutionary algorithm (VDNA-EA) to implement self-learning of a class of Takagi-Sugeno (T-S) fuzzy controllers. The fuzzy controllers use T-S fuzzy rules with linear consequent, the generalized input fuzzy sets, Zadeh fuzzy logic and operators, and the generalized defuzzifier. The fuzzy controllers are proved to be nonlinear proportional-integral (PI) controllers with variable gains. The fuzzy rules are discovered automatically and the design parameters in the input fuzzy sets and the linear rule consequent are optimized simultaneously by the VDNA-EA. The VDNA-EA uses the VDNA encoding method that stemmed from the structure of the VDNA to encode the design parameters of the fuzzy controllers. We use the frameshift decoding method of the VDNA to decode the DNA chromosome into the design parameters of the fuzzy controllers. In addition, the gene transfer operation and bacterial mutation operation inspired by a microbial evolution phenomenon are introduced into the VDNA-EA. Moreover, frameshift mutation operations based on the DNA genetic operations are used in the VDNA-EA to add and delete adaptively fuzzy rules. Our encoding method can significantly shorten the code length of the DNA chromosomes and improve the encoding efficiency. The length of the chromosome is variable and it is easy to insert and delete parts of the chromosome. It is suitable for complex knowledge representation and is easy for the genetic operations at gene level to be introduced into the VDNA-EA. We show how to implement the new method to self-learn a T-S fuzzy controller in the control of a nonlinear system. The fuzzy controller can be constructed automatically by the VDNA-EA. Computer simulation results indicate that the new method is effective and the designed fuzzy controller is satisfactory. © 2003 Wiley Periodicals, Inc. [source] Fuzzy reasoning based on generalized fuzzy If-Then rulesINTERNATIONAL JOURNAL OF INTELLIGENT SYSTEMS, Issue 10 2002Yang Xu This paper focuses on a fuzzy reasoning method based on a generalized If-Then rule. Firstly, the antecedent and the consequent of an If-Then rule are considered and expressed as a component of a kind of binary L -type fuzzy relation on the product of the universes of discourse and the range of definition for a certain fuzzy attribute. Then a generalized extension principle based on this L -type fuzzy relation (FR-GEP) is constructed. Moreover, the paper gives a detailed description of this generalized If-Then rule using 20 well-known common implication operators in the framework of the composition of L -type fuzzy relations. Consequently, an L -type binary fuzzy reasoning method based on this generalized If-Then rule is established according to FR-GEP. © 2002 Wiley Periodicals, Inc. [source] The utility of immature reticulocyte fraction as an indicator of erythropoietic response to altitude training in elite cyclistsINTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, Issue 1p2 2010V. S. NADARAJAN Summary Altitude training is sometimes employed by elite endurance athletes to improve their sea level performance. This improvement results from the increased red cell mass consequent upon the boost in erythropoietin (EPO) level that occurs as a response to the relatively hypoxic environment at high altitudes. We measured serum EPO levels together with various red cell and reticulocyte parameters including immature reticulocyte fraction (IRF) in eight national track-endurance cyclists, resident at sea-level, prior to and upon return from an altitude of approximately 1905 m. Reticulocytes and soluble transferrin receptor (sTfR) were significantly increased with reduction in ferritin levels immediately on return from high altitude indicating increased erythropoietic activity. IRF in particular showed a significant peak immediately on return but decline to sub-baseline levels by day 9, and recovery to baseline by day 16. Our results indicate that IRF is a sensitive marker of erythropoietic status in athletes undergoing altitude training and subsequent loss of EPO stimuli on return to sea level. [source] Bladder wall injury consequent to total hip replacement: Delayed presentationINTERNATIONAL JOURNAL OF UROLOGY, Issue 7 2006ULRICH KALDENBACH Abstract, Bladder injury, although rare, may be traumatic or iatrogenic. We report a patient with greatly delayed presentation of bladder injury after a total hip replacement. [source] The Accession of the UK to the EC: A Welfare AnalysisJCMS: JOURNAL OF COMMON MARKET STUDIES, Issue 3 2002Michael Gasiorek This article provides a decomposition of the welfare impact on the UK arising from the changes in manufacturing trade consequent upon joining the EC. The methodology employed is that of computable general equilibrium (CGE) modelling, where the underlying model is based on trade under imperfect competition with firms producing under conditions of increasing returns to scale. CGE models can be seen as providing numerical illustrations of theory, or as empirical tools providing estimates of policies. A second aim of this article is then to asses the extent to which CGE models can be used as serious tools of policy analysis. We examine this by assessing the success of the model in replicating counterfactual outcomes. The results indicate (i) that the model does reasonably well in replicating complex reality and that such models can be empirically useful; (ii) that a substantial portion of the welfare impact is attributed to distortions associated with imperfect competition, and that the impact is potentially quite large. [source] Seasonal patterns of growth, expenditure and assimilation in juvenile Atlantic salmonJOURNAL OF ANIMAL ECOLOGY, Issue 6 2002Wayne Jones Summary 1We report a modelling study of a data-set describing the growth of individual Atlantic salmon (Salmo salar L.) parr in the Girnock Burn (Scotland). A development of the compensatory growth model due to Broekhusien et al. (1994) was fitted to these data by numerical optimization. 2The model uses carbon mass as a surrogate for an energy currency. This mass is divided into structure and reserve components, so as to describe decoupled changes in length and wet-weight. 3Using the same parameters for all fish, our model explained 83% of the variability in length and weight at age. Adding a single additional parameter for each individual enabled the model to explain over 96% of length and weight variability. 4Weak negative correlation between size at first capture and within-study growth argues against genetic causality of observed growth variability. 5The energetic basis of our model enables us to infer time-series of net assimilation and basal maintenance rates for the observed individuals. Maximal growth occurs early in the season when high assimilation is accompanied by low temperatures and maintenance rates. In late season, continuing high assimilation is balanced by high maintenance rates consequent on summer temperatures. [source] | |||||||||||||||||||||||||||||||||||||||||||