Complex Response (complex + response)

Distribution by Scientific Domains


Selected Abstracts


Complex responses to culture conditions in Pseudomonas syringae pv. tomato DC3000 continuous cultures: The role of iron in cell growth and virulence factor induction

BIOTECHNOLOGY & BIOENGINEERING, Issue 5 2010
Beum Jun Kim
Abstract The growth of a model plant pathogen, Pseudomonas syringae pv. tomato DC3000, was investigated using a chemostat culture system to examine environmentally regulated responses. Using minimal medium with iron as the limiting nutrient, four different types of responses were obtained in a customized continuous culture system: (1) stable steady state, (2) damped oscillation, (3) normal washout due to high dilution rates exceeding the maximum growth rate, and (4) washout at low dilution rates due to negative growth rates. The type of response was determined by a combination of initial cell mass and dilution rate. Stable steady states were obtained with dilution rates ranging from 0.059 to 0.086,h,1 with an initial cell mass of less than 0.6,OD600. Damped oscillations and negative growth rates are unusual observations for bacterial systems. We have observed these responses at values of initial cell mass of 0.9,OD600 or higher, or at low dilution rates (<0.05,h,1) irrespectively of initial cell mass. This response suggests complex dynamics including the possibility of multiple steady states. Iron, which was reported earlier as a growth limiting nutrient in a widely used minimal medium, enhances both growth and virulence factor induction in iron-supplemented cultures compared to unsupplemented controls. Intracellular iron concentration is correlated to the early induction (6,h) of virulence factors in both batch and chemostat cultures. A reduction in aconitase activity (a TCA cycle enzyme) and ATP levels in iron-limited chemostat cultures was observed compared to iron-supplemented chemostat cultures, indicating that iron affects central metabolic pathways. We conclude that DC3000 cultures are particularly dependent on the environment and iron is likely a key nutrient in determining physiology. Biotechnol. Bioeng. 2010;105: 955,964. © 2009 Wiley Periodicals, Inc. [source]


Sediment transmission and storage: the implications for reconstructing landform development

EARTH SURFACE PROCESSES AND LANDFORMS, Issue 1 2010
R. C. Chiverrell
Abstract The late Holocene (last 3000 years) development of the lower Ribble valley (northwest England) displays evidence for a complex response to a sediment recharge event forced by land-use change induced increases in erosion and sediment delivery. The deposition of fluvial sediments during the late Holocene was restricted to a series of reaches or depocenters separated by zones with no sediment accumulation constrained by older glacial and fluvial terrain. Apparent reach-wide correlations of fluvial terraces break down under the scrutiny applied by comprehensive and extensive radiocarbon control. Bayesian testing of relative order models show that large-scale geomorphological changes, e.g. the progression from one terrace level to another, were time transgressive between different depocenters. The different histories of sediment delivery and storage are probably a function of local- and process-scale variations in these depocenters, and reflect (dis)connectivity relationships within a reach in propagating a basin-scale change (superslug) in the sediment regime. Disconnectivity in the depositional regime through a fluvial reach limits what we can reconstruct in terms of sediment budgets, but radiocarbon dating of multiple palaeochannels offers considerable potential for landform-based research to uncover rates of change within individual depocenters. Copyright © 2009 John Wiley & Sons, Ltd. [source]


GEOMORPHIC AND STRATIGRAPHIC COMPLEXITY: HOLOCENE ALLUVIAL HISTORY OF UPPER WOLLOMBI BROOK, AUSTRALIA

GEOGRAFISKA ANNALER SERIES A: PHYSICAL GEOGRAPHY, Issue 1 2008
WAYNE D. ERSKINE
ABSTRACT. Holocene and post-European settlement alluvial histories of three nested drainage basins were reconstructed from detailed litho- and chronostratigraphy of cut and fill terraces and flood-plains in the upper Wollombi Brook catchment. Fernances Creek (13.8 km2) valley fill consisted of intercalated thin mud sheets deposited in ephemeral swamps and thick sand sheets deposited by discontinuous channels. Dairy Arm (39.8 km2) valley fill was more complex, with inset alluvial fills in the upper basin and overlapping vertically stacked fills in the lower basin. However, correlative lithostrati-graphic units were not found on all tributaries. Furthermore, basal radiocarbon dates on the last inset fill of four tributaries did not overlap, allowing for plus or minus twice the standard deviation of the reported ages. Wollombi Brook (341 km2) valley fill was also complex, with longitudinally discontinuous units, most of which were not found in the two tributaries. Upstream late Holocene channel incision was coeval with downstream chain of ponds because sediment generated by incision was stored in the intervening valley. Historical channel incision occurred between 1838 and 1867 on Fernances Creek at a locally steeper section of valley floor during the period of peak population and frequent floods immediately after a road crossing was constructed, but coincided with a catastrophic flood on Dairy Arm (June 1949) and on Wollombi Brook (1927). Lack of correlative litho- and chronostrati-graphic units plus out-of-phase post-European incision indicate that stratigraphic complexity is a function of geomorphic complexity due to the operation of geomorphic thresholds and complex response. [source]


Respiratory chain deficiency slows down cell-cycle progression via reduced ROS generation and is associated with a reduction of p21CIP1/WAF1

JOURNAL OF CELLULAR PHYSIOLOGY, Issue 1 2006
Matthias Schauen
We have used HeLa cells without mitochondrial DNA (,0 -cells) and transient ,0 -phenocopies, obtained from wild-type cells by short-term treatment with ethidium bromide, to analyze how the absence of a functional mitochondrial respiratory chain slows down proliferation. We ruled out an energetic problem (ATP/ADP content) as well as defective synthesis of pyrimidine, iron-sulfur clusters or heme as important causes for the proliferative defect. Flow cytometric analysis revealed that reactive oxygen species were reduced in ,0 -cells and in ,0 -phenocopies, and that, quite unusually, all stages of the cell cycle were slowed down. Specific quenching of mitochondrial ROS with the ubiquinone analog MitoQ also resulted in slower growth. Some important cell-cycle regulators were reduced in ,0 -cells: cyclin D3, cdk6, p18INK4C, p27KIP1, and p21CIP1/WAF1. In the ,0 -phenocopies, the expression pattern did not fully duplicate the complex response observed in ,0 -cells, and mainly p21CIP1/WAF1 was downregulated. Activities of the growth regulatory PKB/Akt and MAPK/ERK-signaling pathways did not correlate with proliferation rates of ,0 -cells and ,0 -phenocopies. Our study demonstrates that loss of a functional mitochondrial electron transport chain inhibits cell-cycle progression, and we postulate that this occurs through the decreased concentration of reactive oxygen species, leading to downregulation of p21CIP1/WAF1. J. Cell. Physiol. 209: 103,112, 2006. © 2006 Wiley-Liss, Inc. [source]


Role of candidate genes in the responses to long-term overfeeding: review of findings

OBESITY REVIEWS, Issue 1 2004
O. Ukkola
Summary An overfeeding experiment conducted with 12 pairs of young male identical twins revealed that genetic factors were likely to play an important role in the response to caloric affluence. Significant intrapair resemblance was observed for the overfeeding-induced changes in body weight, fat mass, abdominal fat, fasting insulin, fasting cholesterol and triglycerides. In an attempt to define the molecular basis of these genotype,energy balance interaction effects, a panel of candidate genes has been investigated. Among the most significant findings, an adipsin polymorphism was associated with increases in body weight, total fat mass and subcutaneous fat in response to overfeeding. In addition, the beta2 adrenergic receptor gene Gln27Glu polymorphism showed a strong association with the gains in body weight and subcutaneous fat. Only a few markers were related to abdominal fat changes and, among them, the adipsin Hinc II polymorphism was associated with both computed tomography (CT)-measured abdominal visceral and total fat. The changes in insulin parameters brought about by long-term overfeeding were influenced most consistently by leptin receptor (LEPR) Gln223Arg and insulin-like growth factor-II Apa I polymorphisms. The LEPR Gln223Arg variant was also associated with the changes in plasma total triglycerides and high-density lipoprotein cholesterol concentrations. Further research with larger sample sizes should make it possible to identify the specific contributions of DNA sequence variations at multiple candidate gene loci in the complex response to chronic positive energy balance. [source]


Topical application of Pya -AKH stimulates lipid mobilization and locomotion in the flightless bug, Pyrrhocoris apterus (L.) (Heteroptera)

PHYSIOLOGICAL ENTOMOLOGY, Issue 1 2002
Dalibor Kodrík
Abstract Two different methods of applying Pya -AKH to long-winged (macropterous) females of the firebug, Pyrrhocoris apterus (Linnaeus) (Heteroptera) were compared: both injection and topical application increased the levels of lipids in the haemolymph and stimulated locomotor activity. Lipid mobilization was maximal when 10 pmol was applied by injection or 40,100 pmol by topical application, with the first significant responses occurring 1.5 h after injection and 2 h after topical application. The highest elevations of lipid concentration in the haemolymph were comparable between the treatments, i.e. 14.36 ± 3.59 mg/mL for injection and 14.43 ± 4.07 mg/mL for topical application. However, these maximal elevations were achieved at different times: 3 h after the injection and 7 h after the topical application. Injection of 10 and 40 pmol of Pya -AKH stimulated locomotor activity with maximal activity 3 h later but, surprisingly, injection of 80 pmol showed no effect initially and than a slight inhibitory effect after 6,8 h. Increased locomotor activity was found after topical application of Pya -AKH, but the response was lower than after injection and appeared later, 5,9 h after the hormone application. In addition, the greatest increase in walking activity required topical application of 300 pmol and was still less dramatic than the response to injection. The stimulatory effect of Pya -AKH on locomotion was positively correlated with its effect on lipid mobilization only for injection of the hormone. It is argued that a stress caused by injection could play a role in the appearance of the complex response to adipokinetic hormone. [source]


Characterization of Ninjurin and TSC22 induction after X-irradiation of normal human skin cells

THE JOURNAL OF DERMATOLOGY, Issue 1 2008
Manabu KOIKE
ABSTRACT The skin is an external organ that is most frequently exposed to radiation. It is important to elucidate the influence of radiation exposure on the skin at the molecular level. To identify radiation-responsive genes in human skin cells, we used microarray technology to examine the effects of irradiation on 641 genes in normal human epidermal keratinocytes at 4 h and 8 h postirradiation with a cytotoxic dose of X-ray (10 Gy). We found that 18 genes were upregulated and 35 genes were downregulated in keratinocytes at 4 h and/or 8 h postirradiation. Ninjurin, whose function remains unknown in keratinocytes, was induced most strongly by X-irradiation. Several known apoptosis-related genes, such as TSC22, were also upregulated. We characterized Ninjurin and TSC22 induction after X-irradiation of normal human skin cells. The induction of the expression of Ninjurin and TSC22 mRNA in keratinocytes following high-dose X-irradiation was confirmed by northern blot analysis. In dermal fibroblasts, Ninjurin, but not TSC22, was induced after X-ray irradiation. The dependence of both gene expression on the status of an apoptosis regulator, p53, was found. In addition, the expression of both mRNA was induced upon treatment with an apoptosis inducer, etoposide. On the other hand, TSC22, but not Ninjurin, was induced and accumulated in keratinocytes upon treatment with an apoptosis inducer, anisomycin. However, in transient expression assay, EYFP-TSC22, as well as EYFP-Ninjurin or EYFP alone, did not induce apoptosis in keratinocytes in contrast to EYFP-GADD45. Taken together, these findings have important implications on the understanding of the mechanism underlying the complex response of skin cells following X-irradiation. [source]


Co-ordinating innate and adaptive immunity to viral infection: mobility is the key

APMIS, Issue 5-6 2009
JEANETTE ERBO CHRISTENSEN
The host counters a viral infection through a complex response made up of components belonging to both the innate and the adaptive immune system. In this report, we review the mechanisms underlying this response, how it is induced and how it is co-ordinated. As cell,cell communication represents the very essence of immune system physiology, a key to a rapid, efficient and optimally regulated immune response is the ability of the involved cells to rapidly shift between a stationary and a mobile state, combined with stringent regulation of cell migration during the mobile state. Through the co-ordinated recruitment of different cell types intended to work in concert, cellular co-operation is optimized particularly under conditions that may involve rare cells. Consequently, a major focus is placed on presenting an overview of the co-operative events and the associated cell migration, which is essential in mounting an efficient host response and co-ordinating innate and adaptive immunity during a primary viral infection. [source]


Redox Properties of Mixed Lutetium/Yttrium Nitride Clusterfullerenes: Endohedral LuxY3,xN@C80(I) (x=0,3) Compounds

CHEMPHYSCHEM, Issue 7 2009
Ján Tarábek Dr.
Abstract The complex redox behavior of mixed lutetium/yttrium nitride clusterfullerenes of the series LuxY3,xN§C80(Ih, x=0,3) (see figure) results in up to three irreversible reduction steps. A reduction mechanism is proposed based on fast-scan and square-wave voltammetry experiments combined with simulations of the voltammograms. The redox behavior of mixed lutetium/yttrium nitride clusterfullerenes of the series LuxY3,xN@C80(Ih, x=0,3) is studied for the first time by means of cyclic voltammetry, fast-scan cyclic voltammetry, square-wave voltammetry, and electron paramagnetic resonance (EPR) spectroelectrochemistry. A reversible single-electron-transfer process, which does not result in an EPR signal, is detected during the anodic oxidation sweep of cyclic voltammetry experiments performed at different temperatures (296 and 360 K). The cathodic reduction sweep reveals a rather complex response for all the four clusterfullerenes,with up to three irreversible reduction steps. By correlating the results of fast-scan and square-wave voltammetry and combining them with simulations of the voltammograms, we are able to propose a reduction mechanism for the LuxY3,xN@C80(I) (x=0,3) fullerenes. [source]


Perceiving and experiencing fictional characters: An integrative account1

JAPANESE PSYCHOLOGICAL RESEARCH, Issue 4 2003
Johan F. Hoorn
Abstract: ,Fictional characters (FCs) and mediated persons in literature, theater, film, art, TV, and digital media fulfill basic psychological functions, although the processes involved remain unspecified. Departing from identification and empathy hypotheses, a new context-sensitive model draws upon similarity studies, empirical aesthetics, persuasion, emotion, and social psychology. The Perceiving and Experiencing Fictional Characters model (PEFiC-model) has three phases. During encoding, observers judge FCs in terms of ethics (good-bad), aesthetics (beautiful-ugly), and epistemics (realistic-unrealistic). Comparison entails appraisals of personal relevance as well as valence towards and (dis)similarity between the dramatis personae and the self. In the response phase, appreciation of FCs is a trade-off between the parallel, unipolar processes of involvement and distance. Intricate involvement-distance conflicts occur when subjective norms disagree with ingroup norms. Furthermore, features participate in multiple (fuzzy) sets (e.g., partly good and partly bad). PEFiC can handle complex responses towards representations of (non-existent) others, such as attractive dissimilarity, the beauty in ugliness, the appeal of negative experiences, and fascination for evil, as well as mixed emotions, ambivalence, and neutral end-states that actually conceal emotional confusion. [source]


Ethanol Dependence Has Limited Effects on GABA or Glutamate Transporters in Rat Brain

ALCOHOLISM, Issue 4 2001
Leslie L. Devaud
Background: Neuroadaptations of GABAergic and glutamatergic systems appear to play an important role in both the acute as well as chronic effects of ethanol. Chronic ethanol intake leads to the development of ethanol tolerance and dependence that is associated with a decrease in GABAergic and an increase in glutamatergic function. The present report assessed the involvement of GABA and glutamate transporters in the chronic ethanol-induced adaptations of these two neuronal systems. Methods: Male and female rats were made ethanol dependent by 2-week administration of ethanol in a liquid diet. Levels of GABA (GAT-1, GAT-3) and glutamate (GLT-1, EAAC-1) transporters were assayed by immunoblotting. Transporter function was assessed by [3H]GABA and [3H]glutamate uptake assays. Results: Ethanol dependence did not alter levels of GABA or glutamate transporters in cerebral cortex compared with pair-fed control values. There were increases in some, but not all, transporter levels in hippocampus and hypothalamus with the development of ethanol dependence. A decreased rate of uptake was observed for GABA in cerebral cortex. There was no change in maximal GABA uptake or in glutamate uptake (Vmax). Conclusions: These results suggest that alterations in GABA and glutamate transporters have only a limited role in neuroadaptations to chronic ethanol intake in rats. However, the observed alterations were region-specific, supporting the complex responses to chronic ethanol exposure and suggesting that neuroadaptations of GABAergic and glutamatergic systems vary across the brain. [source]


Epidermal growth factor-dependent enhancement of axonal regeneration in the pond snail Lymnaea stagnalis: Role of phagocyte survival

THE JOURNAL OF COMPARATIVE NEUROLOGY, Issue 4 2005
Petra M. Hermann
Abstract Peripheral nerve injury triggers complex responses from neuronal as well as from multiple nonneuronal cell types. These responses are coordinated by a wide spectrum of secreted and nonsecreted factors, including growth factors, cytokines, and cell adhesion molecules. These molecules originate from different sources and act both locally at the site of injury as well as centrally at the location of the neuronal cell bodies. One of the signal systems frequently implicated in this process is the epidermal growth factor (EGF) family and its receptors. Expression of members of this family as well as that of EGF-receptors is upregulated in different cell types after peripheral nerve injury. However, the functional significance of this response is unclear. Using a simple invertebrate model system (Lymnaea stagnalis), the present study implicates the EGF/EGF-receptor system in the survival of ionized calcium-binding adaptor molecule 1 (Iba1)-positive phagocytes that reside in the nervous system. We show that inhibiting the EGF-signaling pathway enhances cell death in this type of cell, an effect paralleled by a substantial reduction in axonal regeneration. Therefore, complementing our previous observation that Lymnaea EGF provides trophic support to axotomized neurons, the present results emphasize the significance of nonneuronal actions of EGF receptor ligands in axonal regeneration. Thus, we add a novel perspective to the ongoing discussion on the functional significance of the EGF signaling system in the injury responses of the nervous system. J. Comp. Neurol. 492:383,400, 2005. © 2005 Wiley-Liss, Inc. [source]