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Comprehensive Identification (comprehensive + identification)
Selected AbstractsThe Sound of Silence: Valuing Acoustics in Heritage ConservationGEOGRAPHICAL RESEARCH, Issue 3 2008PENNY O'CONNOR Abstract This paper explores the reasons behind the omission of historic acoustic values from heritage assessments in Australia. Best practice dictates that all cultural heritage values associated with significant places should be assessed in order to make informed conservation and management decisions. However, the multi-sensory nature of aesthetics has been reframed in guidance documentation in ways that run counter to the primary frame. Conventions that have developed around the way places are assessed also work against comprehensive identification of values. As a result, the consideration of aesthetics in cultural heritage is limited to contemporary visual qualities. Furthermore, because the assessment of historic value takes a diachronic rather than synchronic approach, we have little knowledge of the places past communities valued for the sounds they experienced there. Research into landscape preference and acoustic ecology highlights the importance of identifying the inherent acoustic dimension of places and the role sound plays in developing a sense of place. Two landscape areas in Western Australia's south-west with historic acoustic values, the Boranup Sand Patch and the Lower Reaches of the Blackwood River, illustrate how historic soundscapes can provide insightful contrasts and resonances with contemporary values, and how vulnerable such places are when the sound of place is overlooked in land management policies. [source] Expanding the Andersen Model: The Role of Psychosocial Factors in Long-Term Care UseHEALTH SERVICES RESEARCH, Issue 5 2002Elizabeth H Bradley Objective. To examine a prevailing conceptual model of health services use (Andersen 1995) and to suggest modifications that may enhance its explanatory power when applied to empirical studies of race/ethnicity and long-term care. Study Setting. Twelve focus groups of African-American (five groups) and white (seven groups) individuals, aged 65 and older, residing in Connecticut during 2000. Study Design. Using qualitative analysis, data were coded and analyzed in NUD-IST 4 software to facilitate the reporting of recurrent themes, supporting quotations, and links among the themes for developing the conceptual framework. Specific analysis was conducted to assess distinctions in common themes between African-American and white focus groups. Data Collection. Data were collected using a standardized discussion guide, augmented by prompts for clarification. Audio taped sessions were transcribed and independently coded by investigators and crosschecked to enhance coding validity. An audit trail was maintained to document analytic decisions during data analysis and interpretation. Principal Findings. Psychosocial factors (e.g., attitudes and knowledge, social norms, and perceived control) are identified as determinants of service use, thereby expanding the Andersen model (1995). African-American and white focus group members differed in their reported accessibility of information about long-term care, social norms concerning caregiving expectations and burden, and concerns of privacy and self-determination. Conclusions. More comprehensive identification of psychosocial factors may enhance our understanding of the complex role of race/ethnicity in long-term care use as well as the effectiveness of policies and programs designed to address disparities in long-term care service use among minority and nonminority groups. [source] Multiplex fluorescence in situ hybridization in identifying chromosome involvement of complex karyotypes in de novo myelodysplastic syndromes and acute myeloid leukemiaINTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY, Issue 1p1 2010W. XU Summary Complex chromosomal aberrations (CCA) can be detected in a substantial proportion of myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML), which are associated with very poor prognosis. Conventional cytogenetics (CC) cannot accurately define the specific alterations in CCA. Multiplex fluorescence in situ hybridization (M-FISH) allows the comprehensive identification of CCA. In this study, M-FISH was used in 16 patients with de novo MDS and 22 with AML with CCA detected by R-banding CC, and revealed 206 aberrations involved all 24 chromosomes, including 73 numerical chromosomal abnormalities and 133 structural abnormalities. The chromosomes most often involved were, by decreasing incidence, 5, 17, 8, 11, 7 and 21 in 57.9%, 55.3%, 44.7%, 36.8%, 34.2% and 34.2% of the cases, respectively. There were 98 unbalanced translocations, which were the most frequently observed aberrations in our study. Derivative chromosome 5 and 8 were implicated most often. The other derivatives were der(11), der(12), der(7), der(14), der(15) and der(17). Fourteen balanced translocations were detected in our series, and the most frequent reciprocal translocations was t(8;21). Fifty-five monosomies, 15 partial deletions, and 18 trisomies were found in all patients. The most frequently observed were ,5/5q,, ,17/17q,, ,7, ,18, ,21, ,19, and trisomy of chromosome 8 and 6. There were some abnormalities that have not been previously described, including two complex t(8;21) and seven unbalanced translocations. M-FISH could refine CCA, find or correct the missed or misidentified aberrations by CC analysis. Our findings confirmed that M-FISH was a powerful molecular cytogenetic tool to characterize complex karyotypes in MDS and AML. [source] Strategies for identifying genes that play a role in spinal cord regenerationJOURNAL OF ANATOMY, Issue 1 2004M. Wintzer Abstract A search for genes that promote or block CNS regeneration requires numerous approaches; for example, tests can be made on individual candidate molecules. Here, however, we describe methods for comprehensive identification of genes up- and down-regulated in neurons that can and cannot regenerate after injury. One problem concerns identification of low-abundance genes out of the 30 000 or so genes expressed by neurons. Another difficulty is knowing whether a single gene or multiple genes are necessary. When microchips and subtractive differential display are used to identify genes turned on or off, the numbers are still too great to test which molecules are actually important for regeneration. Candidates are genes coding for trophic, inhibitory, receptor and extracellular matrix molecules, as well as unknown genes. A preparation useful for narrowing the search is the neonatal opossum. The spinal cord and optic nerve can regenerate after injury at 9 days but cannot at 12 days after birth. This narrow window allows genes responsible for the turning off of regeneration to be identified. As a next step, sites at which they are expressed (forebrain, midbrain, spinal cord, neurons or glia, intracellular or extracellular) must be determined. An essential step is to characterize proteins, their levels of expression, and their importance for regeneration. Comprehensive searches for molecular mechanisms represent a lengthy series of experiments that could help in devising strategies for repairing injured spinal cord. [source] Newly identified respiratory viruses in children with asthma exacerbation not requiring admission to hospitalJOURNAL OF MEDICAL VIROLOGY, Issue 8 2010Katherine E. Arden Abstract There are few data describing the comprehensive identification in and influence of newly identified respiratory viruses on asthma exacerbations. Most studies focus on inpatients. In this preliminary study, the point prevalence and the associations of picornavirus species described recently and human bocavirus (HBoV) with the recovery from exacerbations in non-hospitalized asthmatic children (median age 5.1 years) were examined. Human rhinoviruses (HRVs) were present in 52.6% of specimens, HBoV-1 was in 7.7%. Viral co-detections occurred in 25.6% of children and were associated (P,=,0.04) with lower asthma quality of life scores upon presentation than were single viral detections. The undifferentiated presence or absence of virus did not influence the severity of asthma or recovery however when virus species were examined individually, specific clinical associations emerged. HRV species C (HRV-Cs) were the viruses most frequently detected as single virus detections. Among 41 genotyped HRVs, more HRV-Cs (n,=,23) were identified than HRV-As (n,=,16) however HRV-A detection was associated (P,=,0.01) with worse asthma symptoms and cough for longer than was HRV-C detection. Larger, PCR-based studies are required to elucidate further the true impact of HRV species in childhood asthma exacerbations of both hospitalized and non-hospitalized cohorts. J. Med. Virol. 82:1458,1461, 2010. © 2010 Wiley-Liss, Inc. [source] Strategy for comprehensive identification of human N -myristoylated proteins using an insect cell-free protein synthesis systemPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 9 2010Takashi Suzuki Abstract To establish a strategy for the comprehensive identification of human N -myristoylated proteins, the susceptibility of human cDNA clones to protein N -myristoylation was evaluated by metabolic labeling and MS analyses of proteins expressed in an insect cell-free protein synthesis system. One-hundred-and-forty-one cDNA clones with N -terminal Met-Gly motifs were selected as potential candidates from ,2000 Kazusa ORFeome project human cDNA clones, and their susceptibility to protein N -myristoylation was evaluated using fusion proteins, in which the N -terminal ten amino acid residues were fused to an epitope-tagged model protein. As a result, the products of 29 out of 141 cDNA clones were found to be effectively N -myristoylated. The metabolic labeling experiments both in an insect cell-free protein synthesis system and in the transfected COS-1 cells using full-length cDNA revealed that 27 out of 29 proteins were in fact N -myristoylated. Database searches with these 27 cDNA clones revealed that 18 out of 27 proteins are novel N -myristoylated proteins that have not been reported previously to be N -myristoylated, indicating that this strategy is useful for the comprehensive identification of human N -myristoylated proteins from human cDNA resources. [source] | |||||||||||||