EVOLUTION, Issue 11 2004
Gregory B. Pauly
Abstract Previous hypotheses of phylogenetic relationships among Nearctic toads (Bufonidae) and their congeners suggest contradictory biogeographic histories. These hypotheses argue that the Nearctic Bufo are: (1) a polyphyletic assemblage resulting from multiple colonizations from Africa; (2) a paraphyletic assemblage resulting from a single colonization event from South America with subsequent dispersal into Eurasia; or (3) a monophyletic group derived from the Neotropics. We obtained approximately 2.5 kb of mitochondrial DNA sequence data for the 12S, 16S, and intervening valine tRNA gene from 82 individuals representing 56 species and used parametric bootstrapping to test hypotheses of the biogeographic history of the Nearctic Bufo. We find that the Nearctic species of Bufo are monophyletic and nested within a large clade of New World Bufo to the exclusion of Eurasian and African taxa. This suggests that Nearctic Bufo result from a single colonization from the Neotropics. More generally, we demonstrate the utility of parametric bootstrapping for testing alternative biogeographic hypotheses. Through parametric bootstrapping, we refute several previously published biogeographic hypotheses regarding Bufo. These previous studies may have been influenced by homoplasy in osteological characters. Given the Neotropical origin for Nearctic Bufo, we examine current distributional patterns to assess whether the Nearctic-Neotropical boundary is a broad transition zone or a narrow boundary. We also survey fossil and paleogeographic evidence to examine potential Tertiary and Cretaceous dispersal routes, including the Paleocene Isthmian Link, the Antillean and Aves Ridges, and the current Central American Land Bridge, that may have allowed colonization of the Nearctic. [source]

PLANT COLONIZATION IN CONDESA NIVATION HOLLOW, SIERRA DE GUADARRAMA (SPANISH CENTRAL SYSTEM)

GEOGRAFISKA ANNALER SERIES A: PHYSICAL GEOGRAPHY, Issue 3 2009
JULIO MUÑOZ JIMÉNEZ
ABSTRACT. The purpose of this study was to determine plant distribution at a nivation hollow located in a Mediterranean high mountain area and to analyse the effects of snow cover, wind exposure, proximity to moisture and the characteristics of the substrate on the vegetation. We analyse these factors and interpret concurrent effects due to recent climate change. The nivation hollow, called Ventisquero de la Condesa, is located at 2258 m a.s.l., 40°47,10,N and 3°58,35,W, in the Sierra de Guadarrama (Madrid, Spain). We established 579 small sample plots in the study area, and grouped them into 29 transects where snow duration, wind exposure, availability of meltwater, geomorphologic instability and surface rockiness were examined directly and indirectly. The types of plants and the number of individuals per species were registered for each plot to establish ecological affinities among the 28 distinguishable species. Six showed the highest level of chionophily while nine showed the lowest adaptation to snow cover duration. A statistical study incorporating other variables applied in the research revealed that wind exposure, moisture capture and the intensity of geomorphologic dynamics have a highly significant correlation with nivation, while surface rockiness is a virtually independent factor. Due to environmental changes caused by recent global warming, several plant species, especially adapted to survival in snow conditions, coexist in the hollow with saxicolous plants that have invaded the site from adjacent grass and shrublands. [source]

CONVERSION TO CHRISTIANITY: THE COLONIZATION OF THE MIND?

INTERNATIONAL REVIEW OF MISSION, Issue 366 2003
Brian Stanley
First page of article [source]

ROLE OF SURFACE WOUNDS AND BROWN ALGAL EPIPHYTES IN THE COLONIZATION OF ASCOPHYLLUM NODOSUM (PHAEOPHYCEAE) FRONDS BY VERTEBRATA LANOSA (RHODOPHYTA),

JOURNAL OF PHYCOLOGY, Issue 3 2009
Caroline M. Longtin
Ascophyllum nodosum (L.) Le Jol. forms extensive beds in wave-sheltered, rocky intertidal habitats on the northwestern Atlantic coast. This fucoid seaweed is host to an obligate red algal epiphyte, Vertebrata lanosa (L.) T. A. Chr. [=Polysiphonia lanosa (L.) Tandy], and two facultative brown algal epiphytes, Elachista fucicola (Velley) Aresch. and Pylaiella littoralis (L.) Kjellm. Although V. lanosa can occur throughout most of the length of host fronds, it largely predominates in midfrond segments. The two brown algal epiphytes are restricted to distal segments. Through field experiments conducted in Nova Scotia, Canada, we tested the hypothesis that surface wounds are required for the colonization of distal segments of host fronds by V. lanosa. Distal tissues normally have a smooth surface because of their young age (A. nodosum fronds grow apically). By creating small wounds that mimicked grazing wounds distributed elsewhere on host fronds, we demonstrated that V. lanosa can colonize distal frond segments during the growth and reproductive season (summer and autumn). Approximately half of the artificial wounds were colonized by V. lanosa during this time. The experimental exclusion of both brown algal epiphytes from distal frond segments did not affect colonization by V. lanosa. Thus, we conclude that the absence of surface irregularities on distal segments of host fronds, specifically small wounds, is the main factor explaining the absence of V. lanosa there. We propose that further experimental work clarifying epiphyte distribution in host beds will enhance our ability to understand the functional role of epiphytes in intertidal ecosystems. [source]

HISTORICAL AND RECENT COLONIZATION OF THE SOUTH FARALLON ISLANDS, CALIFORNIA, BY NORTHERN FUR SEALS (CALLORHINUS URSINUS)

MARINE MAMMAL SCIENCE, Issue 2 2001
Peter Pyle
[source]

Climate Change and Moving Species: Furthering the Debate on Assisted Colonization

CONSERVATION BIOLOGY, Issue 5 2007
MALCOLM L. HUNTER JR
No abstract is available for this article. [source]

BIODIVERSITY RESEARCH: Population expansion in an invasive grass, Microstegium vimineum: a test of the channelled diffusion model

DIVERSITY AND DISTRIBUTIONS, Issue 5 2010
Nathaniel P. Miller
Abstract Aim, The greatest biodiversity impact of non-native plant species is caused by rapid expansion of colonist populations. Unfortunately, invasion has rarely been documented in real time at a population scale, and demographic mechanisms of invasion remain unclear. Our goal is to describe real-time expansion of populations, using channelled diffusion as a null model. Location, The study examined three populations of the invasive annual grass Microstegium vimineum in mature second-growth forests of south-eastern Ohio and nearby West Virginia, USA. Methods, Distributions were recorded in belt transects perpendicular to population edges over a period of 3 years. A second group of belt transects documented spread along five types of potential movement corridor. Observed changes in distribution were compared with predictions from a diffusion model. A seed-sowing experiment tested seed availability, microsite quality and proximity to potential movement corridors as factors controlling population spread. Results, Population boundaries showed little change over the study period. Colonization was limited by propagule availability over distances as little as 0.25 m, and to a lesser extent by litter cover. Populations did not advance along several potential movement corridors including unpaved roads, off-road vehicle trails and footpaths. Advance was observed along deer trails and stream courses but did not conform to the wave-form distribution predicted by diffusion theory. During the study, seeds were moved out of experimental plots by sheet flow and minor flooding events along small streams. Main conclusion, At a population level, invasion is driven by processes that are episodic in time and non-random in space , probably a common condition in non-native plant species. Spatially realistic models are likely to be more useful than diffusive models in managing invasions at these scales. [source]

Colonization of the wasteland in County Durham, 1100-1400

ECONOMIC HISTORY REVIEW, Issue 1 2003
H.M. Dunsford
First page of article [source]

Colonization of nascent, deep-sea hydrothermal vents by a novel Archaeal and Nanoarchaeal assemblage

ENVIRONMENTAL MICROBIOLOGY, Issue 1 2006
Elizabeth A. McCliment
Summary Active deep-sea hydrothermal vents are areas of intense mixing and severe thermal and chemical gradients, fostering a biotope rich in novel hyperthermophilic microorganisms and metabolic pathways. The goal of this study was to identify the earliest archaeal colonizers of nascent hydrothermal chimneys, organisms that may be previously uncharacterized as they are quickly replaced by a more stable climax community. During expeditions in 2001 and 2002 to the hydrothermal vents of the East Pacific Rise (EPR) (9°50,N, 104°17,W), we removed actively venting chimneys and in their place deployed mineral chambers and sampling units that promoted the growth of new, natural hydrothermal chimneys and allowed their collection within hours of formation. These samples were compared with those collected from established hydrothermal chimneys from EPR and Guaymas Basin vent sites. Using molecular and phylogenetic analysis of the 16S rDNA, we show here that at high temperatures, early colonization of a natural chimney is dominated by members of the archaeal genus Ignicoccus and its symbiont, Nanoarchaeum. We have identified 19 unique sequences closely related to the nanoarchaeal group, and five archaeal sequences that group closely with Ignicoccus. These organisms were found to colonize a natural, high temperature protochimney and vent-like mineral assemblages deployed over high temperature outflows within 92 h. When compared phylogenetically, several of these colonizing organisms form a unique clade independent of those found in mature chimneys and low-temperature mineral chamber samples. As a model ecosystem, the identification of pioneering consortia in deep-sea hydrothermal vents may help advance the understanding of how early microbial life forms gained a foothold in hydrothermal systems on early Earth and potentially on other planetary bodies. [source]

The influence of arbuscular mycorrhizal colonization and environment on root development in soil

EUROPEAN JOURNAL OF SOIL SCIENCE, Issue 4 2003
D. Atkinson
Summary The production of fine roots is one of the principal means by which carbon, fixed during photosynthesis, enters the soil, and quantifying the production for particular combinations of environmental and biotic factors is important for predicting the sequestration of carbon in the soils of grassland ecosystems. Arbuscular mycorrhizal fungi (AMF) can have a major effect on the production of roots, and we studied how colonization by AMF affects the lifespan of roots. Twenty per cent of control roots of Trifolium repens survived for longer than 42 days whereas 37% survived that long in AMF-colonized plants. The overall survival of the roots of Lolium perenne was less than in T. repens: around 10% of roots survived beyond 42 days and this was not affected by AMF colonization. Previous studies have shown that lifespans of roots can be affected by temperature. We tested the hypothesis that these observations are linked to a change in the morphology of the root system caused by temperature and also by AMF. We found that inoculation with AMF in a microcosm study using Plantago lanceolata grown at various temperatures, with and without AMF, showed no clear effect of AMF on branching patterns. Temperature had a significant effect on total lengths, numbers and branching rates of some higher orders of roots. Total lengths of both secondary and tertiary roots grown at 27°C were about double those of plants grown at 15°C. Colonization by AMF tended to reduce this effect. Evidently the effect of colonization by AMF on root lifespan depends on the species. Increased branching, and thus a greater proportion of ephemeral roots, was responsible for shortening the lives of the roots at increased temperature, which suggests a strong link between lifespan and morphology. [source]

REPLICATED EVOLUTION OF INTEGRATED PLASTIC RESPONSES DURING EARLY ADAPTIVE DIVERGENCE

EVOLUTION, Issue 4 2006
Kevin J. Parsons
Abstract Colonization of a novel environment is expected to result in adaptive divergence from the ancestral population when selection favors a new phenotypic optimum. Local adaptation in the new environment occurs through the accumulation and integration of character states that positively affect fitness. The role played by plastic traits in adaptation to a novel environment has generally been ignored, except for variable environments. We propose that if conditions in a relatively stable but novel environment induce phenotypically plastic responses in many traits, and if genetic variation exists in the form of those responses, then selection may initially favor the accumulation and integration of functionally useful plastic responses. Early divergence between ancestral and colonist forms will then occur with respect to their plastic responses across the gradient bounded by ancestral and novel environmental conditions. To test this, we compared the magnitude, integration, and pattern of plastic character responses in external body form induced by shallow versus open water conditions between two sunfish ecomorphs that coexist in four postglacial lakes. The novel sunfish ecomorph is present in the deeper open water habitat, whereas the ancestral ecomorph inhabits the shallow waters along the lake margin. Plastic responses by open water ecomorphs were more correlated than those of their local shallow water ecomorph in two of the populations, whereas equal levels of correlated plastic character responses occurred between ecomorphs in the other two populations. Small but persistent differences occurred between ecomorph pairs in the pattern of their character responses, suggesting a recent divergence. Open water ecomorphs shared some similarities in the covariance among plastic responses to rearing environment. Replication in the form of correlated plastic responses among populations of open water ecomorphs suggests that plastic character states may evolve under selection. Variation between ecomorphs and among lake populations in the covariance of plastic responses suggests the presence of genetic variation in plastic character responses. In three populations, open water ecomorphs also exhibited larger plastic responses to the environmental gradient than the local shallow water ecomorph. This could account for the greater integration of plastic responses in open water ecomorphs in two of the populations. This suggests that the plastic responses of local sunfish ecomorphs can diverge through changes in the magnitude and coordination of plastic responses. Although these results require further investigation, they suggest that early adaptive evolution in a novel environment can include changes to plastic character states. The genetic assimilation of coordinated plastic responses could result in the further, and possibly rapid, divergence of such populations and could also account for the evolution of genes of major effect that contribute to suites of phenotypic differences between divergent populations. [source]

CONTEMPORARY PATTERNS IN A HISTORICAL CONTEXT: PHYLOGEOGRAPHIC HISTORY OF THE PIPEVINE SWALLOWTAIL, BATTUS PHILENOR (PAPILIONIDAE)

EVOLUTION, Issue 5 2003
James A. Fordyce
Abstract We examined mitochondrial DNA (mtDNA) variation in pipevine swallowtail butterflies (Battus philenor) from throughout its extant range to provide a historical, phylogeographical context for ecological studies of the disjunct population in California. We evaluate current hypotheses regarding host plant use, behavior, and mimetic relationships of B. philenor populations and generate alternative hypotheses. Compared to populations throughout the rest of the species' range, California populations are ecologically distinct in that they lack mimics, lay significantly larger clutches of eggs, and exclusively use a unique, endemic larval host plant. Analysis of molecular variance, tests of population differentiation, and nested clade analysis of mtDNA variation indicate that, despite low levels of population genetic structure across the species' range, there is evidence of recent range expansion from presumed Pleistocene refuge(s) in southeastern North America. Colonization of California appears to have been a recent event. This phylogeographic investigation also suggests that the evolution of life-history adaptations to a novel larval host has occurred rapidly in California and the lack of mimics in California may be attributable to the recency of colonization. [source]

The Helicobacter pylori plasticity region locus jhp0947,jhp0949 is associated with duodenal ulcer disease and interleukin-12 production in monocyte cells

FEMS IMMUNOLOGY & MEDICAL MICROBIOLOGY, Issue 2 2004
Ramon De Jonge
Abstract Colonization with Helicobacter pylori always results in chronic gastritis, which is controlled by infiltration of mononuclear cells and the subsequent release of cytokines like interleukin (IL)-12. To identify H. pylori factors involved in inducing cytokine production in mononuclear cells, a random H. pylori mutant library was screened for the inability to induce IL-12 production in monocyte THP-1 cells. Of the 231 random mutants screened, one mutant (M1) showed a consistent twofold decrease in the amount of IL-12 induction compared to the parental strain 1061 (P<0.01). Further characterization of mutant M1 revealed that the kanamycin resistance cassette had integrated in the jhp0945 gene, which is situated in an H. pylori strain-specific plasticity region. Three reference strains possessing this plasticity region induced significantly higher amounts of IL-12 when compared to the H. pylori 26695 reference strain, which does not possess this plasticity region. The role in disease outcome of jhp0945 as well as the neighbouring plasticity region genes jhp0947 and jhp049 was assessed in a Dutch population cohort. Firstly, the presence of jhp0947 was completely linked with that of jhp0949 and was roughly associated with jhp0945 (P=0.072), but not with the cag pathogenicity island (PAI) (P=0.464). The presence of the jhp0947 and jhp0949 genes, but not of jhp0945, was significantly associated with duodenal ulcer disease when compared to gastritis (P=0.027). Therefore, the jhp0947,jhp0949 locus may be a novel putative H. pylori marker for disease outcome independent of the cag PAI. [source]

Simultaneous detection of the establishment of seed-inoculated Pseudomonas fluorescens strain DR54 and native soil bacteria on sugar beet root surfaces using fluorescence antibody and in situ hybridization techniques

FEMS MICROBIOLOGY ECOLOGY, Issue 1 2000
Peter Stephensen Lübeck
Abstract Colonization at sugar beet root surfaces by seedling-inoculated biocontrol strain Pseudomonas fluorescens DR54 and native soil bacteria was followed over a period of 3 weeks using a combination of immunofluorescence (DR54-targeting specific antibody) and fluorescence in situ hybridization (rRNA-targeting Eubacteria EUB338 probe) techniques with confocal laser scanning microscopy. The dual staining protocol allowed cellular activity (ribosomal number) to be recorded in both single cells and microcolonies of strain DR54 during establishment on the root. After 2 days, the population density of strain DR54 reached a constant level at the root basis. From this time, however, high cellular activity was only found in few bacteria located as single cells, whereas all microcolony-forming cells occurring in aggregates were still active. In contrast, a low density of strain DR54 was observed at the root tip, but here many of the bacteria located as single cells were active. The native population of soil bacteria, comprising a diverse assembly of morphologically different forms and size classes, initiated colonization at the root basis only after 2 days of incubation. Hence the dual staining protocol allowed direct microscopic studies of early root colonization by both inoculant and native soil bacteria, including their differentiation into active and non-active cells and into single or microcolony-forming cells. [source]

Pluronics' influence on pseudomonad biofilm and phenazine production

FEMS MICROBIOLOGY LETTERS, Issue 1 2009
Lindsay Housley
Abstract Colonization of roots by Pseudomonas chlororaphis O6 (PcO6) involves root surface coverage through surface motility and biofilm formation. Root colonization and the production of antifungal phenazines are important in the ability of the bacterium to protect plants against pathogens. In this in vitro study we report that both biofilm formation and phenazine production are differentially influenced by nutrition and the presence of polyethylene oxide/polypropylene oxide triblock copolymer surfactants (Pluronics). Such surfactants are used for many purposes including agricultural formulations. Four Pluronics differing in molecular weight and in hydrophobic/hydrophilic proportions had distinct effects on biofilm formation and secondary metabolite production, although each increased surface motility, termed swarming, to a similar extent. These findings show that Pluronics had specific metabolic impacts on the bacterium, where both up- and downregulation was achieved depending on the medium and the Pluronic composition. In environmental and agricultural settings, Pluronics may have unanticipated effects on soil microorganisms, while in bioprocessing these effects may be leveraged to regulate metabolite yield. [source]

Colonization of beech leaves by two endophytic fungi in northern Japan

FOREST PATHOLOGY, Issue 2 2000
N. Sahashi
Summary Leaves of Japanese beech (Fagus crenata) were collected monthly during the vegetation period at five sites in the Tohoku district in Japan to isolate endophytic fungi. Leaves were also collected only once at two additional sites. Two endophytic fungi were dominant, a Discula species and a sterile mycelium. This result strongly suggests that these two fungi are generally associated with leaves of the Japanese beech at different sites. At most sites the isolation frequency of Discula sp. was greatest in June and gradually decreased from July to October whereas the isolation frequency of the sterile mycelium increased during the vegetation period and remained at a high isolation frequency in October. Spores of Discula sp. were released for a very short time in late May, just after the disappearance of the snow cover on the forest floor. These spores may be important for the infection of newly sprouting leaves. [source]

Manipulation of flooding and arbuscular mycorrhiza formation influences growth and nutrition of two semiaquatic grass species

FUNCTIONAL ECOLOGY, Issue 6 2000
S. P. Miller
Abstract 1Two semiaquatic grasses, Panicum hemitomon Schultes and Leersia hexandra Schwartz, were grown for 12 weeks in sterilized soil in experimental mesocosms, with and without the addition of arbuscular mycorrhizal (AM) fungal inoculum (as nonsterilized soil), under the following rooting-zone flood regimes: waterlogged (W), free-draining (D), beginning W and ending D (W,D), and beginning D and ending W (D,W). The purpose of the experiment was to determine whether these controlled water regimes affected both colonization of wetland grasses by AM fungi and the effects of the colonization on various plant parameters. 2Water regime, addition of inoculum, and their interaction were highly significant effects on total and proportion of root length colonized by AM fungi. Trends were very similar for the two grass species. Colonization was less and plants smaller in the W and W,D than in the D and D,W treatments. The viability of mycorrhiza at the end of the experiment, as measured by vital staining techniques, was not affected by changes in water level. 3Colonized plants in all water level treatments showed an improvement in phosphorus (P) nutrition over noncolonized plants. Colonized grasses of both species gained consistently more P per plant and had greater tissue P concentrations, with the greatest P concentration in the most heavily colonized plants (from the D and D,W treatments). 4The effect of flooding on the mycorrhizal association depended largely on the extent to which the association was already established when the flooding occurred. Flooding reduced the initiation of colonization either directly or indirectly, but once the fungi were established in the roots they were able to maintain and expand with the growing root system. [source]

Temporal dynamics and nestedness of an oceanic island bird fauna

GLOBAL ECOLOGY, Issue 4 2006
Ermias T. Azeria
ABSTRACT Aim, To examine temporal variation in nestedness and whether nestedness patterns predict colonization, extinction and turnover across islands and species. Location, Dahlak Archipelago, Red Sea. Method, The distributions of land birds on 17 islands were recorded in two periods 30 years apart. Species and islands were reordered in the Nestedness Temperature Calculator, software for assessing degrees of nestedness in communities. The occupancy probability of each cell, i.e. species,island combinations, was calculated in the nested matrix and an extinction curve (boundary line) was specified. We tested whether historical and current nested ranks of species and islands were correlated, whether there was a relationship between occupancy probability (based on the historical data) and number of extinctions or colonizations (regression analyses) and whether the boundary line could predict extinctions and colonizations (chi-square analyses). Results, Historical and current nested ranks of islands and species were correlated but changes in occupancy patterns were common, particularly among bird species with intermediate incidence. Extinction and turnover of species were higher for small than large islands, and colonization was negatively related to isolation. As expected, colonizations were more frequent above than below the boundary line. Probability of extinction was highest at intermediate occupancy probability, giving a quadratic relationship between extinction and occupancy probability. Species turnover was related to the historical nested ranks of islands. Colonization was related negatively while extinction and occupancy turnover were related quadratically to historical nested ranks of species. Main conclusions, Some patterns of the temporal dynamics agreed with expectations from nested patterns. However, the accuracy of the predictions may be confounded by regional dynamics and distributions of idiosyncratic, resource-limited species. It is therefore necessary to combine nestedness analysis with adequate knowledge of the causal factors and ecology of targeted species to gain insight into the temporal dynamics of assemblages and for nestedness analyses to be helpful in conservation planning. [source]

Naturally Occurring Regulatory T cells (CD4+, CD25high, FOXP3+) in the Antrum and Cardia are Associated with Higher H. pylori Colonization and Increased Gene Expression of TGF-,1

HELICOBACTER, Issue 4 2008
Arne Kandulski
Abstract Background:Helicobacter pylori causes gastric inflammation. Despite the induction of H. pylori -specific B- and T cells, the immune response is not sufficient to clear the infection. Regulatory T cells (Treg cells) suppress the activation and proliferation of antigen-specific T cells and mediate immunologic tolerance. FOXP3 was shown to be expressed in a subset of Treg cells known as ,naturally occurring Treg cells'. These cells have not been sufficiently studied in context to H. pylori -induced inflammation in human gastric mucosa. Materials and methods: The study included 76 patients stratified according to the presence of H. pylori. Gene expression levels of FOXP3, transforming growth factor (TGF)-,1, and interleukin-10 were analyzed by quantitative real-time polymerase chain reaction in biopsies from gastric antrum, corpus, and cardia. FOXP3 expression was also analyzed by immunohistochemistry. Differences in expression levels were analyzed by comprehensive statistical analyses and correlated with clinical and histomorphologic parameters. Results:H. pylori -positive patients revealed a 19- to 25-fold induction of FOXP3 transcript levels in antrum and cardia (p < .02). FOXP3 transcript levels correlated positively with inflammation (p < .04) and TGF-,1 transcript levels (p < .001). Furthermore, a positive correlation between FOXP3+ Treg cells and H. pylori colonization was demonstrated. Conclusion: This study demonstrates that H. pylori -induced gastritis is associated with a recruitment of naturally occurring FOXP3+ Treg cells that correlates with the degree of bacterial colonization and mucosal TGF-,1 expression. Together, these data support the hypothesis that naturally FOXP3+ Treg cells play a role in the lifelong persistence of H. pylori infection in humans. [source]

Helicobacter pylori Infection in the Cat: Evaluation of Gastric Colonization, Inflammation and Function

HELICOBACTER, Issue 1 2001
Kenneth W. Simpson
Background. Further elucidation of the consequences of Helicobacter pylori infection on gastric mucosal inflammation and gastric secretory function would be facilitated by an animal model that is susceptible to infection with H. pylori, is broadly similar in gastric physiology and pathology to people, and is amenable to repeated non-invasive evaluation. The goal of this study was to examine the interrelationship of bacterial colonization, mucosal inflammation and gastric secretory function in cats with naturally acquired H. pylori infection. Materials and Methods. Twenty clinically healthy cats with naturally acquired H. pylori infection (cagA,, picB) and 19 Helicobacter -free cats were evaluated. Gastric colonization was determined by tissue urease activity, light microscopy, culture and PCR. The mucosal inflammatory response was evaluated by light microscopy, and by RT-PCR of the pro-inflammatory cytokines IL-1,, IL-1,, IL-8 and TNF-, in gastric mucosa. Gastric secretory function was assessed by measuring pentagastrin-stimulated acid secretion, fasting plasma gastrin, and antral mucosal gastrin and somatostatin immunoreactivity. Results. H. pylori colonized the pylorus, fundus and cardia in similar density. Bacteria were observed free in the lumen of gastric glands and were also tightly adherent to epithelial cells where they were associated with microvillus effacement. Mononuclear inflammation, lymphoid follicle hyperplasia, atrophy and fibrosis were observed primarily in H. pylori -infected cats, with the pylorus most severely affected. Neutrophilic and eosinophilic infiltrates, epithelial dysplasia, and up-regulation of mucosal IL-1, and IL-8 were observed solely in infected cats. Fasting plasma gastrin concentrations and pentagastrin-stimulated acid output were similar in both infected and uninfected cats. There was no relationship of bacterial colonization density or gastric inflammation to plasma gastrin concentrations or gastric acid output. Conclusions. The pattern of colonization and the mucosal inflammatory response in cats with naturally acquired H. pylori are broadly similar to those in infected people, particularly children, and non-human primates. The upregulation of IL-8 in infected cats was independent of cagA and picB. Our findings argue against a direct acid-suppressing effect of H. pylori on the gastric secretory-axis in chronically infected cats. Abbreviations: RT-PCR, reverse transcriptase polymerase chain reaction, HLO; Helicobacter -like organisms. [source]

Dental health and oral mutans streptococci in 2,4-year-old Estonian children

INTERNATIONAL JOURNAL OF PAEDIATRIC DENTISTRY, Issue 2 2007
JANA OLAK
Objective., The aim of this study was to assess the dental health and presence of mutans streptococci (MS) in 2,4-year-old Estonian children. Methods., The dental health of 472 2,4-year-old children was examined using a mirror and a penlight in 14 daycare centres representing seven communities around Estonia. The mean (± SD) age of the children was 41.4 ± 4.1 months (n = 222). Plaque samples of 222 children were employed to determine the presence of MS using the Dentocult® SM Strip mutans test. Results., Caries was diagnosed in 42% of the children, and the average (± SD) dmft index was 1.6 ± 2.5, ranging from 1.1 ± 1.2 in Tartu to 2.4 ± 3.1 in Võru. The proportion of caries-free children decreased from 82% in the younger to 63% in the older group (P = 0.001). Among the tested subjects, 58% were colonized with MS, and those with caries were colonized more often than children with no visible caries (80% and 51%, respectively; P = 0.001). Conclusions., The prevalence of dental caries in Estonian 2,4-year-olds is higher than in the Nordic countries, but similar to other Baltic nations. Colonization by MS was associated with dental caries. [source]

Morocco's Migration Experience: A Transitional Perspective1

INTERNATIONAL MIGRATION, Issue 4 2007
Hein De Haas
ABSTRACT Using a ,transitional' perspective on migration, which combines three theoretical approaches on dynamic development-migration linkages, this paper interprets the evolution of migration within, from, and to Morocco over the twentieth century. Colonization and the incorporation of rural areas, along with a certain level of socio-economic development, have spurred internal and international wage labour migration both within Morocco and from Morocco to Europe. Migration seems to be the result of development rather than the lack of development. Populations from highly marginalized regions were less likely to participate in migration than populations from the three, moderately enclosed "migration belts" which had established traditions of pre-modern, largely circular migration. At the onset of large-scale emigration in the 1960s, the spatial patterns of labour migration were significantly infuenced by colonial bonds with Spain and France, selective labour recruitment, and Moroccan selective passport issuance policies. However, the influence of such policies rapidly decreased due to the effects of migration-facilitating networks. Increasingly restrictive policies coincided with a growing reliance on family migration, permanent settlement, undocumented migration, and the exploration of new migration itineraries, and had no success in reducing migration levels. Alongside patterns of decentralizing internal migration, a spatial diffusion of international out-migration has expanded beyond the historical migration belts in response to new labour opportunities in southern Europe. Persistent demand for migrant labour, along with demographic factors and increasing aspirations, suggest that migration over formally closed borders is likely to remain high in the near future. However, in the longer term, out-migration might decrease and Morocco could increasingly develop into a migration destination for migrants from sub-Saharan Africa, a transition process which may already have een set in motion. [source]

Colonization of Residents with Antimicrobial-Resistant Pathogens in Skilled Care Facilities

JOURNAL OF AMERICAN GERIATRICS SOCIETY, Issue 12 2002
Paul J.Drinka MD
No abstract is available for this article. [source]

Colonization of Skilled-Care Facility Residents with Antimicrobial-Resistant Pathogens

JOURNAL OF AMERICAN GERIATRICS SOCIETY, Issue 12 2001
Michael Westerman MD
No abstract is available for this article. [source]

Colonization of the chicken reproductive tract and egg contamination by Salmonella

JOURNAL OF APPLIED MICROBIOLOGY, Issue 2 2004
J. De Buck
[source]

Australian biogeographical connections and the phylogeny of large genera in the plant family Myrtaceae

JOURNAL OF BIOGEOGRAPHY, Issue 7 2003
Pauline Y. Ladiges
Abstract Aim To compare the phylogeny of the eucalypt and melaleuca groups with geological events and ages of fossils to discover the time frame of clade divergences. Location Australia, New Caledonia, New Guinea, Indonesian Archipelago. Methods We compare published molecular phylogenies of the eucalypt and melaleuca groups of the plant family Myrtaceae with geological history and known fossil records from the Cretaceous and Cenozoic. Results The Australasian eucalypt group includes seven genera, of which some are relictual rain forest taxa of restricted distribution and others are species-rich and widespread in drier environments. Based on molecular and morphological data, phylogenetic analyses of the eucalypt group have identified two major clades. The monotypic Arillastrum endemic to New Caledonia is related in one clade to the more species-rich Angophora, Corymbia and Eucalyptus that dominate the sclerophyll vegetation of Australia. Based on the time of rifting of New Caledonia from eastern Gondwana and the age of fossil eucalypt pollen, we argue that this clade extends back to the Late Cretaceous. The second clade includes three relictual rain forest taxa, with Allosyncarpia from Arnhem Land the sister taxon to Eucalyptopsis of New Guinea and the eastern Indonesian archipelago, and Stockwellia from the Atherton Tableland in north-east Queensland. As monsoonal, drier conditions evolved in northern Australia, Arnhem Land was isolated from the wet tropics to the east and north during the Oligocene, segregating ancestral rain forest biota. It is argued also that the distribution of species in Eucalyptopsis and Eucalyptus subgenus Symphyomyrtus endemic in areas north of the stable edge of the Australian continent, as far as Sulawesi and the southern Philippines, is related to the geological history of south-east Asia-Australasia. Colonization (dispersal) may have been aided by rafting on micro-continental fragments, by accretion of arc terranes onto New Guinea and by land brought into closer proximity during periods of low sea-level, from the Late Miocene and Pliocene. The phylogenetic position of the few northern, non-Australian species of Eucalyptus subgenus Symphyomyrtus suggests rapid radiation in the large Australian sister group(s) during this time frame. A similar pattern, connecting Australia and New Caledonia, is emerging from phylogenetic analysis of the Melaleuca group (Beaufortia suballiance) within Myrtaceae, with Melaleuca being polyphyletic. Main conclusion The eucalypt group is an old lineage extending back to the Late Cretaceous. Differentiation of clades is related to major geological and climatic events, including rifting of New Caledonia from eastern Gondwana, development of monsoonal and drier climates, collision of the northern edge of the Australian craton with island arcs and periods of low sea level. Vicariance events involve dispersal of biota. [source]

Colonization of an island volcano, Long Island, Papua New Guinea, and an emergent island, Motmot, in its caldera lake.

JOURNAL OF BIOGEOGRAPHY, Issue 11-12 2001

Abstract Biogeographical context Long Island, in Vitiaz Strait, is 55 km north of New Guinea, 60 km from Umboi Island and 125 km from New Britain. After its explosive caldera-forming eruption in about 1645, Long is being recolonized by animals and plants. Through renewed volcanic activity in the 1950s a new island emerged from Long's caldera lake, 4 km from the nearest lake shore and was recreated by eruptions in 1968. Long Island thus provides the opportunity to study a nested pair of natural colonization sequences. The geological background, eruptive history, course and results of the seventeenth century eruption, and the geographical features and climate of Long Island are summarized. Existing knowledge of Long's recolonization, confined almost entirely to surveys of its avifauna in 1933 and 1972, is reviewed. The geological history of Motmot is outlined, and published knowledge of its colonization by animals and plants from 1968 to 1988 is summarized. The 1999 expedition and aims An expedition to Long Island and Motmot in 1999 set out to investigate the hitherto little-known flora and present vertebrate fauna of Long Island and to survey the entire flora and fauna of Motmot for comparison with the results of previous surveys. The methods used in the 1999 survey are described, and the papers setting out the results briefly introduced. [source]

Colonization of an island volcano, Long Island, Papua New Guinea, and an emergent island, Motmot, in its caldera lake.

JOURNAL OF BIOGEOGRAPHY, Issue 11-12 2001

Abstract Aim Long Island erupted catastrophically in c. 1645 with the probable destruction of its entire biota. While several expeditions have visited the island since, no survey of its flora has been published. In 1968 a small island, Motmot, emerged from its caldera lake. Motmot has been surveyed several times but not since 1988. The aim of this study was to investigate the colonization by vascular plants of this interesting nested pair of islands. Location Long Island is 55 km off the northeast coast of Papua New Guinea (PNG) and Motmot lies in its fresh water caldera lake, Lake Wisdom, c. 4 km from the rim of the surrounding volcano. Methods We conducted a complete survey of the vascular plants on Motmot and made ad hoc collections on Long Island, including surveying three small plots, over 15 days in 1999. In addition, we incorporated data from several collections lodged at the regional herbarium in Lae. Data on seed dispersal syndromes and plant habit were obtained from the literature. Results We recorded 305 species of vascular plants from Long Island, but most trees were small and the species diversity was low. Motmot was still very sparsely vegetated and only forty-five species of vascular plants were found. Communities on Motmot were unspecialized and common species widespread. The flora of Motmot was not significantly different from that on Long Island in terms of the number of species amongst higher taxa, seed dispersal syndromes or plant habits. Main conclusions Low rainfall combined with very porous soils may be responsible for the small stature and low diversity of the forest on Long Island's caldera rim. The absence of specialist littoral species on Motmot, as a result of its landlocked situation, is probably responsible for the very slow species recruitment on the island. This community appears to be functionally important for colonization by comparison with other small volcanic islands in a marine situation. [source]

Colonization of an island volcano, Long Island, Papua New Guinea, and an emergent island, Motmot, in its caldera lake.

JOURNAL OF BIOGEOGRAPHY, Issue 11-12 2001

Abstract Location, Aim Long Island, 55 km north of New Guinea, erupted explosively in the seventeenth century and has been recolonized by animals and plants. Effectively in 1968, an island, Motmot, emerged from Long's 13 km-diameter fresh-water caldera lake, about 4 km from the nearest shore. A nested pair of colonization sequences is thus available for study. Long Island has been recolonized by birds, and Motmot has been colonized, presumably from the surrounding ring of Long Island. In order to monitor further colonization of Long Island following bird surveys in 1933 and 1972, and to survey the birds of Motmot, Motmot and the western parts of Long were visited for 15 days in 1999. Results In the last 66 years one species probably has become extinct; in the past 27 years three species have colonized and three, present in 1972, were not recorded in 1999. We assess the present number of nonmigrant land bird species as fifty. On Motmot, we recorded eight species, three breeding, and found two more only as prey remains (three raptor species were seen on the island). Main conclusions Long Island's avifauna still has youthful features. Species with good colonizing ability predominate, and there are no island-endemic subspecies. There is a persistently high proportion of supertramps; none of the ten supertramps has been replaced by later-colonizing ,high-S' species. A quasi-equilibrium of about fifty species, fourteen fewer than the predicted equilibrium number for the island, has persisted for at least seven decades and may be seen as that appropriate to the present prolonged stage of Long's floral and vegetational development. Compared with the younger avifauna of Krakatau, Long's avifauna has a higher proportion of nectarivores, predators of large invertebrates and small vertebrates, and ground-foraging insectivore-herbivores, and a lower proportion of the insectivore-frugivore guild. Motmot's small avifauna consists predominantly of species obtaining subsistence from outside the island. [source]

Analytical epidemiology of periodontitis

JOURNAL OF CLINICAL PERIODONTOLOGY, Issue 2005
Luisa N. Borrell
Abstract Aims: To review the literature related to the analytical epidemiology of periodontitis generated over the past decade. This review does not deal with descriptive epidemiologic studies of the prevalence, extent and severity of periodontitis with respect to global geography, but focuses exclusively on analytical epidemiology issues, including the challenges posed by the use of different case definitions across studies, current theories and models of disease progression, and risk factors associated with the onset and progression of periodontitis. Methods: Relevant publications in the English language were identified after Medline and PubMed database searches. Findings and conclusions: There is a conspicuous lack of uniformity in the definition of periodontitis used in epidemiologic studies, and findings from different research groups are not readily interpretable. There is a lack of studies that specifically address the distinction between factors responsible for the onset of periodontitis versus those affecting its progression. Colonization by specific bacteria at high levels, smoking, and poorly controlled diabetes have been established as risk factors for periodontitis, while a number of putative factors, including specific gene polymorphisms, have been identified in association studies. There is a clear need for longitudinal prospective studies that address hypotheses emerging from the cross-sectional data and include established risk factors as covariates along with new exposures of interest. Intervention studies, fulfilling the "targeting" step of the risk assessment process, are particularly warranted. Obvious candidates in this context are studies of the efficacy of elimination of specific bacterial species and of smoking cessation interventions as an alternative to the traditional broad anti-plaque approach in the prevention and control of periodontitis. Ideally, such studies should have a randomized-controlled trial design. [source]