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Chicken Carcasses (chicken + carcass)

Distribution by Scientific Domains

Life Sciences	66%

Selected Abstracts

Discrimination of Arcobacter butzleri isolates by polymerase chain reaction-mediated DNA fingerprinting

LETTERS IN APPLIED MICROBIOLOGY, Issue 2 2002
H.I. Atabay
Aims:,The objective of this study was to subtype Arcobacter butzleri isolates using RAPD-PCR. Methods and Results:,Thirty-five A. butzleri isolates obtained from chicken carcasses were examined. PCR-mediated DNA fingerprinting technique with primers of the variable sequence motifs was used to detect polymorphism within the isolates. Eleven distinct DNA profiles were obtained as follows: Of the 35 strains, 10 as profile 4; seven as profile 1; five as profile 3; three as profiles 2 and 9; two as profile 10; one as profiles 5, 6, 7, 8 and 11. Conclusions:,Chicken carcasses sold in markets were found to be contaminated with several different strains of A. butzleri. RAPD-PCR technique was found to be a useful technique for distinguishing A. butzleri isolates. Significance and Impact of the Study:,The presence of several different A. butzleri strains on chicken carcasses may indicate multiple sources of contamination. The epidemiological role of A. butzleri in human and animal diseases should be investigated further. [source]

Prevalence of Salmonella enterica serovars and genovars from chicken carcasses in slaughterhouses in Spain

JOURNAL OF APPLIED MICROBIOLOGY, Issue 5 2007
R. Capita
Abstract Aims: To determine the prevalence of Salmonella enterica serovars in chicken carcasses in slaughterhouses in Spain and to examine genotypic relations among these serovars. Methods and Results: A total of 336 chicken carcasses were collected from six slaughterhouses in Northwestern Spain. Salmonellae were isolated (ISO-6579-1993), serotyped, phage-typed, ribotyped and antibiotyped against 20 antibiotics. Salmonella strains were detected in 60 (17·9%) carcasses. Isolates belonged to nine different serotypes, with Salm. Enteritidis being the most common. Three strains (5%) were resistant to one antibiotic and 24 (40%) were multi-resistant (to more than one antibiotic). The most frequently encountered resistances were to sulphamides, fluoroquinolones and tetracycline. Ribotyping was able to differentiate isolates of the same serotype and phage type. Conclusions: The Salmonella serotypes and phage types detected are among those most frequently associated with human diseases in Spain. The large percentage of antimicrobial resistant strains is a matter for concern. A high genetic relationship between strains from different slaughterhouses was found. Significance and Impact of the Study: This study provides detailed information about Salmonella isolates from poultry in Spain. It emphasizes the importance of controlling this pathogen in poultry products, and suggests the need for more prudent use of antibiotics. [source]

Quality and Microbial Population of Cornish Game Hen Carcasses as Affected by Electron Beam Irradiation

JOURNAL OF FOOD SCIENCE, Issue 7 2006
C. Gomes
ABSTRACT:, We evaluated the chemical and microbiological quality of Cornish game hen carcasses irradiated up to 7 kGy with a 10 MeV linear accelerator (dual beam configuration). Eighty frozen and vacuum packaged (approximately 0.45 kg) Cornish game hens (Gallus domesticus) were irradiated and stored in low-density polyethylene bags at 4 ± 1 °C for 21 d; nonirradiated chickens served as controls. Fat oxidation (in terms of malonaldehyde content) increased with storage time and dose for all chicken parts analyzed (breast, thigh, and skin). As expected, the skin had the highest level of fat oxidation while the breast samples had the lowest. Oxidation level in all samples exposed to 2 kGy reached a maximum on day 14. Sensory evaluation showed that irradiation caused significant textural toughening, and increased the redness of raw chicken meat. In terms of overall quality and aroma, lipid oxidation was not a major problem since it was not detected by the panelists. Irradiation significantly reduced the total viable microbial counts (TVC) in the breast and thigh samples. Exposure to 3-kGy dose decreased the TVC by 0.3-log cycles on the surface of the skin. In less than 14 d, the nonirradiated chicken carcasses had counts greater than 6 log CFU/50 cm2, while the 2 and 3 kGy irradiated samples reached these numbers only after 21 d of storage. Samples irradiated at 7 kGy had consistently the lowest counts (2.5 log CFU/50 cm2) throughout storage time. This study shows that irradiation up to 7 kGy and refrigerated storage (4 °C) inhibits microbial growth and extends shelf life of Cornish game hens without affecting consumers' acceptability. [source]

Methods to Detect the Occurrence of Various Indicator Bacteria on the Surface of Retail Poultry in Spain

JOURNAL OF FOOD SCIENCE, Issue 2 2002
R. Capita
ABSTRACT: Contamination levels (cfu/g and cfu/cm2) of indicator microorganisms in retail broiler chicken carcasses in León (Spain) were investigated. Counts (log10 cfu/g) were 5.19, 3.04, 2.73, 3.38, and 3.16 for total aerobic counts (TAC), Enterobacteriaceae, coliforms determined by the standard VRBA method (coliforms-VRBA), coliforms determined by the Hydrophobic Grid Membrane Filter method (coliforms-HGMF), and Escherichia coli (HGMF method), respectively. These values fit into the microbiological criteria for poultry meat consulted. A low correlation coefficient was found between TAC and Enterobacteriaceae counts (r = 0.308; P = 0.053) and between coliforms-VRBA and coliforms-HGMF counts (r = 0.398; P= 0.048). The determination method had a significant influence on the coliform counts obtained. All broiler chicken carcasses harbored E. coli biotype I. E. coli biotype II was detected in 20% of the samples. The HGMF method was not completely specific for detecting E. coli since 11.25% of false positive colonies were found. [source]