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Chemiluminescence Method (chemiluminescence + method)
Selected AbstractsSuperoxide radical generation and Mn- and Cu-Zn superoxide dismutases activities in human leukemic cellsHEMATOLOGICAL ONCOLOGY, Issue 1 2003Masahiko Kato Abstract Mn- and Cu-Zn superoxide dismutase (SOD) activities and generation of superoxide radicals (O) were assessed in leukemic cells from 10 patients with acute myeloid or monocytic leukemia (AML) and 10 patients with acute lymphoblastic leukemia (ALL), using a sensitive, specific chemiluminescence method. Leukemic cells were classified according to the French,American,British classification. M4 AML cells from two patients produced some O upon stimulation with opsonized zymosan (OZ), phorbol myristate acetate (PMA) or N-formyl-methionyl-leucyl-phenylalanine (FMLP), but less than normal granulocytes or monocytes. M5b AML cells from one patient produced as much O in response to these stimulants as normal monocytes. No O generation was induced in other types of leukemic cells. Total SOD activity in AML cells was significantly greater in normal granulocytes, but was only half of the activity in ALL cells. Mn-SOD in AML cells was very low or undetectable. These results suggest that except in M5b cells, decreased O production may contribute to susceptibility to infections in AML patients. Decreased Mn-SOD activity in AML cells may predispose them to oxidative stress. Copyright © 2002 John Wiley & Sons, Ltd. [source] Suppressive effects of cyclosporine A on neutrophils and T cells may be related to therapeutic benefits in patients with steroid-resistant ulcerative colitisINFLAMMATORY BOWEL DISEASES, Issue 1 2002Kenji Ina Abstract An intravenous infusion of cyclosporine A (CsA) shows clinical benefits in patients with steroid-resistant ulcerative colitis (UC). To clarify its mechanisms, we investigated the ability of CsA to inhibit the functions of neutrophils and T cells. The cytotoxic activity by mucosal T cells was analyzed by anti-CD3-triggered cytotoxicity after lamina propria mononuclear cells were cultured with recombinant interleukin (IL)-2. The chemotactic response, the generation of superoxide, and the production of chemokines, IL-8, and macrophage inflammatory protein-1, by neutrophils were examined using a multiple-well chamber assay, a chemiluminescence method, and an enzyme-linked immunosorbent assay (ELISA), respectively. Mucosal chemokine activity was determined by an ELISA using the organ culture supernatant of mucosal biopsy tissues. Pretreatment with CsA caused consistent inhibitions of cytotoxic activity by mucosal T cells and chemotactic migration, superoxide generation, and chemokine production by neutrophils mostly in a dose-dependent manner. In patients who received an intravenous infusion of CsA, mucosal chemokine activity decreased after therapy in parallel with decreases in the numbers of neutrophils and mononuclear cells in the biopsy tissues. These results suggest that suppressive effects of CsA on neutrophils and T cells may be related to therapeutic benefits in patients with steroid-resistant UC. [source] Seroprevalence of human T-lymphotropic virus type 1 and 2 in Korean blood donorsJOURNAL OF MEDICAL VIROLOGY, Issue 10 2008So-Yong Kwon Abstract The seroprevalence rate of human T-lymphotropic virus (HTLV) among the Korean blood donor population was studied to determine whether screening for HTLV should be implemented. A total of 15,173 serum samples collected from June to July 2006 at five Blood Centers which are located closely to Japan geographically, where the prevalence of HTLV is known to be high, were tested. Serological screening was done by a chemiluminescence method. Samples reactive repeatedly on serological screening were confirmed further by Western blot, line immunoassay, nested polymerase chain reaction and sequencing of proviral DNA. Six samples tested reactive with the serological assay showing a reactive rate of 0.004%. Among the six samples, one sample was confirmed as HTLV-1 positive, giving a confirmed reactive rate of 0.007%. Based on the results of this study, an extended study will be conducted to evaluate whether introduction of HTLV screening is necessary in Korea. J. Med. Virol. 80:1864,1867, 2008. © 2008 Wiley-Liss, Inc. [source] Determination of tsumacide residues in vegetable samples using a flow-injection chemiluminescence methodLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 4 2007Haiyan Liu Abstract A sensitive, simple and rapid flow-injection chemiluminescence (FI,CL) method is described to determine tsumacide pesticide residue based on the CL reaction of the alkaline degradation product of tsumacide with acidic KMnO4 when rhodamine 6G was present. Under the optimum conditions, the relative CL intensity is linear with the concentration of tsumacide in the range of 2.0 × 10,3,0.20 mg/L. The detection limit is 6.6 × 10,4 mg/L (3,) and the relative standard deviation for 2.0 × 10,2 mg/L tsumacide solution was 2.28% (intra-day) and 4.85% (inter-day). The proposed method has been applied to determine the residue of tsumacide in vegetable samples and the recovery test is very satisfactory. Copyright © 2007 John Wiley & Sons, Ltd. [source] Determination of norfloxacin using a terbium-sensitized electrogenerated chemiluminescence methodLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 1 2006Shi-Lv Chen Abstract A simple electrogenerated chemiluminescence (ECL) analysis method for the determination of norfloxacin (NFLX) is reported. It is based on ECL produced by Na2SO3, which is sensitized by the Tb,NFLX complex. The relative ECL intensity of the Tb3+,NFLX,Na2SO3 system is proportional to the amount of NFLX. The optimized experimental conditions were investigated. The linear range and detection limit for NFLX were 1.0 × 10,10,8.0 × 10,7 mol/L and 2.8 × 10,11 mol/L, respectively. This method was successfully applied to the determination of NFLX in a capsule. NFLX in urine can be directly detected without pretreatment or separation. Copyright © 2005 John Wiley & Sons, Ltd. [source] Chemiluminescence determination of pharmacologically active compounds by capillary electrophoresisLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 6 2005Suqin Han Abstract A simple and rapid capillary electrophoresis with direct chemiluminescence method has been developed for the determination of five natural pharmacologically active compounds including rutin, protocatechuic aldehyde, chlorogenic acid, luteolin and protocatechuic acid. The luminol as a component of the separation electrolyte buffer was introduced at the head of the separation capillary. The separation of five compounds was carried out in a fused-silica capillary with 15.0 mmol/L tetraborate, 1.0 mmol/L SDS and 0.42 mmol/L luminol (pH 8.5). The analytes was determined by enhancing the chemiluminescence of luminol with 0.13 mmol/L K3Fe(CN)6 in 0.05 mol/L NaOH, which was introduced at the post-column stage. The voltage applied was 16 kV. Under the optimum conditions, the analytes were separated within 10 min. The excellent linearity was obtained over two to three orders of magnitude with a detection limit (signal:noise = 3) of 0.012,0.055 µmol/L for all five analytes. The method was successfully used in the analysis of pharmaceutical and biological samples, and the assay results were satisfactory. Copyright © 2005 John Wiley & Sons, Ltd. [source] Flow-injection,chemiluminescence method for the determination of penicillin G potassiumLUMINESCENCE: THE JOURNAL OF BIOLOGICAL AND CHEMICAL LUMINESCENCE, Issue 4-5 2005Wei Cao Abstract The degradation product of penicillin G potassium can react with potassium permanganate in acidic medium and produce chemiluminescence, which is greatly enhanced by formaldehyde. The optimum conditions for this chemiluminescent reaction were studied in detail using a flow-injection system. The experiments indicated that under optimum conditions, the chemiluminescence intensity was linearly related to the concentration of penicillin G potassium within the range 1.0 × 10,7,1.0 × 10,5 g/mL, with a detection limit (3,) of 7 × 10,8 g/mL. The relative standard deviation was 1.0% for 4.0 × 10,7 g/mL penicillin G potassium solution (n = 11). This method has the advantages of simple operation, fast response and high sensitivity. The method was successfully applied to the analysis of penicillin G potassium in raw medicines. Copyright © 2005 John Wiley & Sons, Ltd. [source] The significance of serum levels of insulin-like growth factor-1 in patients with prostate cancerBJU INTERNATIONAL, Issue 1 2000R. Kurek Objectives,To compare the serum levels of insulin-like growth factor-1 (IGF-1) in patients with prostate cancer and in control patients with no malignancy, and to evaluate any possible influence of testicular androgen withdrawal on the level of IGF-1 in patients with prostate cancer. Patients and methods,IGF-1 was measured in serum samples from 238 patients using both a chemiluminescence method and a radio-immunoassay. From a subgroup of 19 patients presenting with newly diagnosed carcinoma of the prostate, IGF-1 and testosterone values were measured before and during the course of testicular androgen with-drawal, achieved by the administration of luteinizing hormone-releasing hormone (LHRH) analogues combined with anti-androgens. Results,There were no significant differences in the mean serum levels of IGF-1 patients with and without prostate cancer (158.6 and 159.1 ng/mL, respect-ively). There were no significant differences in mean IGF-1 levels before and after antiandrogen therapy; the mean (median, sd, range) levels of testosterone (µg/L) and IGF-1 (ng/mL) before androgen withdrawal were 4.81 (4.84, 1.26, 3.11,6.93) and 157.1 (152.5, 26.7, 122.8,195.1). After androgen withdrawal the corresponding values were 0.303 (0.218, 0.24, 0.13,0.81) and 169.7 (31.7, 168.6, 124.9,227.6). A linear regression analysis (P = 0.76) and Spearman rank order correlation test (correlation coefficient ,0.0613, P = 0.64) showed no association between levels of testosterone and IGF-1. Freeze and thaw cycles applied to the samples had no effect on the IGF-1 values measured. Conclusions,There was no significant association between IGF-1 serum levels and prostate cancer. Short-term androgen withdrawal using LHRH anal-ogues combined with anti-androgens had no effect on the levels of IGF-1. [source] | ||||||||||