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Chemical Cues (chemical + cue)

Distribution by Scientific Domains

Life Sciences	86%
Polymers and Materials Science	6%
Earth and Environmental Science	6%

Distribution within Life Sciences

Ecology & Organismal Biology	27%
Entomology	15%

Selected Abstracts

Female Attraction to Conspecific Chemical Cues in the Palmate Newt Triturus helveticus

ETHOLOGY, Issue 8 2005
Jean Secondi
Although chemosignals are largely used in sexual communication in urodeles, olfactometer studies in newts provided contrasting results about the sex specificity of female behavioural responses. Because long-range sexual advertisement is believed to be costly, some species might restrain this activity to close interactions with conspecifics. We tested chemical-mediated sexual attraction in female palmate newt (Triturus helveticus) by measuring the attraction to male and female odours in a linear water olfactometer. Unexpectedly, females were attracted towards conspecifics regardless of sex. They did not show attraction towards Limnaea stagnalis, a common sympatric aquatic gastropod. These results do not support the use of long-range male sexual signalling in the palmate newt. Instead, conspecific attraction is likely to promote aggregation of males and females in breeding ponds. Observations in the field and in the laboratory tend to support the aggregative behaviour of this species. We discuss the possible function of conspecific attraction in this context. Heading towards any conspecific would increase the probability of finding potential mates. Chemical cues do not need to be sex-specific at that stage so that long-range sexual advertisement might be unnecessary. This work emphasizes the need for studies investigating the evolutionary relationships between sexual signalling systems and population-distribution patterns. [source]

Population and Species Divergence of Chemical Cues that Influence Male Recognition of Females in Desmognathine Salamanders

ETHOLOGY, Issue 7 2003
Paul Verrell
Growing evidence indicates that males may be more discriminating of mating partners than often has been assumed. In the North American Ocoee dusky salamander, Desmognathus ocoee (Plethodontidae: Desmognathinae), sexual incompatibility among conspecific populations is high in encounters staged in the laboratory, at least in part because males fail to recognize ,other' females as appropriate targets for courtship. I used Y-mazes to test the hypothesis that males of D. ocoee discriminate between substrate-borne chemical cues produced by ,own' (homotypic) and ,other' (heterotypic) females. Males of four populations discriminated in favor of substrates soiled by homotypic females over clean (control) substrates (expt 1), suggesting that females produce chemical cues of sociosexual significance to males. Furthermore, males from these populations discriminated in favor of substrates soiled by homotypic females vs. substrates soiled by heterotypic females (expt 2), both conspecific and heterospecific (D. carolinensis and D. orestes). Thus, differences among populations and species in female chemical cues appear to affect the chemotactic responses of males. I suggest that, together with differences in behavioral signals and responses exhibited during courtship, differences in female chemical cues likely contribute to sexual incompatibility among populations and taxa of desmognathine salamanders. [source]

Direct Microfabrication of Topographical and Chemical Cues for the Guided Growth of Neural Cell Networks on Polyamidoamine Hydrogels

MACROMOLECULAR BIOSCIENCE, Issue 8 2010
Gabriel Dos Reis
Abstract Cell patterning is an important tool for organizing cells in surfaces and to reproduce in a simple way the tissue hierarchy and complexity of pluri-cellular life. The control of cell growth, proliferation and differentiation on solid surfaces is consequently important for prosthetics, biosensors, cell-based arrays, stem cell therapy and cell-based drug discovery concepts. We present a new electron beam lithography method for the direct and simultaneous fabrication of sub-micron topographical and chemical patterns, on a biocompatible and biodegradable PAA hydrogel. The localized e-beam modification of a hydrogel surface makes the pattern able to adsorb proteins in contrast with the anti-fouling surface. By also exploiting the selective attachment, growth and differentiation of PC12 cells, we fabricated a neural network of single cells connected by neuritis extending along microchannels. E-beam microlithography on PAA hydrogels opens up the opportunity of producing multifunctional microdevices incorporating complex topographies, allowing precise control of the growth and organization of individual cells. [source]

Female Attraction to Conspecific Chemical Cues in the Palmate Newt Triturus helveticus

Chemical cues and binary individual recognition in the hermit crab Pagurus longicarpus

JOURNAL OF ZOOLOGY, Issue 1 2004
Francesca Gherardi
Abstract The behaviour exhibited by the hermit crab Pagurus longicarpus in response to an empty shell varied in the presence of cues from conspecific individuals according to its familiarity or not with them. This binary discrimination was independent of the conspecific's relative size and was based on chemical signatures, an ability that this species shares with a few other aquatic invertebrates. From our results, olfaction appeared to be the dominant sensory channel in P. longicarpus' binary discrimination, but the combination of two signal components from visual and olfactory channels resulted in the enhancement of the response displayed by the receiver. Besides, crabs reacted differently when exposed to their own odour than to the odour of familiar (as well as unfamiliar) conspecifics, suggesting that recognition in this species can be more refined than a binary discrimination and that chemical ,badges' may be attributes of individual crabs. [source]

Cyclomorphosis in Daphnia lumholtzi induced by temperature

FRESHWATER BIOLOGY, Issue 2 2000
Peder M. Yurista
Summary 1Cyclomorphosis is a well known phenomenon in Daphnia that involves a regular, seasonal, or induced change in body allometry. Long helmets and tail spines were induced in laboratory cultures of Daphnia lumholtzi with temperature of 31 °C as the proximal cue (temperature of locally occurring peak abundance in Kentucky Lake). The effect was greater in embryos than juveniles or adults exposed to the temperature cue. 2The temperature cue appears to have a threshold value (animals cultured at 25 or 28 °C did not develop elongated helmets or spines). The helmet and spine length receded both with D. lumholtzi kept at a constant 31 °C temperature and when water temperature was decreased. 3The induced helmet in this experiment (0.66 mm, 1.0 mm animal) was significantly longer than values reported in the literature for induction by planktivorous fish kairomones (0.25 mm, 1.2 mm animal). The strong response to a proximal cue of temperature may require the second weaker chemical cue for maintenance. It is suggested that a synergistic explanation with two cues may be more appropriate for cyclomorphosis induction and maintenance in Daphnia lumholtzi that could be tested with further studies. [source]

Resistance of apple trees to Cydia pomonella egg-laying due to leaf surface metabolites

ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, Issue 1 2008
Nadia Lombarkia
Abstract During host plant selection and particularly after alighting on a plant, chemical cues from the plant surface influence an insect's acceptance of the plant and, subsequently, its egg-laying behaviour. Primary metabolites in the phylloplane may be more important than hitherto known. We have shown that soluble carbohydrates, such as glucose, fructose, and sucrose, and sugar alcohols, such as sorbitol, quebrachitol, and myo -inositol, can be detected by insects after contacting the plant and that they positively influence egg-laying of the codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), on apple trees. We addressed the question whether a lack of these substances could also explain apple tree resistance to C. pomonella in terms of reduced egg-laying. Leaf surface washings were collected in an apple orchard by spraying water on the resistant cultivar X65-11 and on the susceptible cultivar P5R50A4. The washings were tested on a nylon cloth on isolated females under no-choice conditions. The washings were analysed and synthetic blends, each consisting of the six metabolites in the proportions established in the leaf surface washings of both cultivars, were then tested for their effect on egg-laying of C. pomonella. Dose,response egg-laying tests were carried out on substrates impregnated with the X65-11 leaf surface blend at 1, 100, 1 000, and 10 000 times the natural dose. Egg-laying behaviour in the bioassays with leaf surface washings of both cultivars closely resembled egg-laying in the orchard. Washings of P5R50A4 stimulated egg-laying to a greater extent than those of X65-11 and the water control. Synthetic blends reduced substrate acceptance and egg-laying, compared to the washings of X65-11. Ratios between components within the blend are responsible for this resistance. In conclusion, quantities and ratios of the six primary metabolites found on the leaf surface may influence host preference of C. pomonella as well as their egg-laying behaviour, thus they may play a role in the trees' resistance to the codling moth. [source]

Induced biotic responses to herbivory and associated cues in the Amazonian ant-plant Maieta poeppigii

ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, Issue 2 2004
Alexander V. Christianini
Abstract Ants inhabiting ant-plants can respond to cues of herbivory, such as the presence of herbivores, leaf damage, and plant sap, but experimental attempts to quantify the dynamic nature of biotic defenses have been restricted to a few associations between plants and ants. We studied the relationship between certain features of the ant-shrub Maieta poeppigii Cogn. (Melastomataceae) and the presence or absence of ant patrolling on the leaf surface in plants occupied by the ant Pheidole minutula Mayr (Hymenoptera: Formicidae). We also carried out field experiments to examine ant behavior following plant damage, and the potential cues that induce ant recruitment. These experiments included clipping of the leaf apex, as well as the presentation of a potential herbivore (live termite worker) and a foliar extract from Maieta on treatment leaves. The presence of ants patrolling the leaves of M. poeppigii is influenced by the number of domatia on the plant. Ant patrolling on the leaves of M. poeppigii was constant throughout a 24 h cycle, but the mean number of patrolling ants decreased from young to mature leaves, and from leaves with domatia to those without domatia. There was an overall increase in the number of ants on experimental leaves following all treatments, compared to control leaves. Visual and chemical cues associated with herbivory are involved in the induction of ant recruitment in the Maieta,Pheidole system. The continuous patrolling behavior of ants, associated with their ability to respond rapidly to foliar damage, may result in the detection and repellence/capture of most insect herbivores before they can inflict significant damage to the leaves. [source]

Physical and chemical cues influencing the oviposition behaviour of Aphidius ervi

ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, Issue 3 2000
Donatella Battaglia
Abstract The oviposition behaviour of the aphid parasitoid Aphidius ervi Haliday is influenced by both chemical and physical cues. Oviposition attack responses were elicited by paint pigments sealed into the tip of a glass capillary tube. Parasitoids reacted to yellow pigments with repeated oviposition attack responses, but they did not react to green pigments. The spectrum of reflected light from the yellow pigments was very similar to that from the ,green' natural host Acyrthosiphon pisum (Harris), with a high proportion of the total radiation energy being emitted in the yellow-orange wavebands (580,660 nm). Pea aphid cornicle secretion also elicited oviposition attack responses, which were not exclusively induced by its pale yellow-green colour. In fact, the oviposition attack response to capillary tips coated with cornicle secretion remained evident under red light conditions, which, in contrast, nearly completely suppressed the response to yellow pigments. Chemical compounds from cornicle secretion do not appear to be involved in parasitoid orientation, even though they stimulate intense oviposition attack responses. Olfactometer experiments showed that the putative kairomone involved acts only at very short range or on contact. Host exuviae, which also elicited strong and persistent oviposition reactions from A. ervi females, appear to be a good alternative source of ovipositional kairomone(s). This work confirms the existence of an aphid cuticular kairomone. [source]

No Plastic Responses to Experimental Manipulation of Sperm Competition per se in a Free-Living Flatworm

ETHOLOGY, Issue 4 2010
Peter Sandner
In the absence of sperm competition evolutionary theory predicts low mating rates and low ejaculate expenditure per mating, and sex allocation theory for simultaneous hermaphrodites predicts a strongly female-biased sex allocation. In the presence of sperm competition a shift towards a more male-biased sex allocation and a higher ejaculate expenditure are predicted. The free-living flatworm Macrostomum lignano has been shown to respond plastically in mating rate, testis size, and sperm transfer to manipulation of the social group size, a proxy of the strength of sperm competition. However, manipulation of social group size may manipulate not only sperm competition, but also other factors, such as food supply and metabolite concentration. In this study we therefore manipulated sperm competition per se by repeatedly exposing individuals to partners that have either mated with rivals or not, while keeping the social group size constant. Our results suggest that M. lignano does not have the ability to detect sperm competition per se, as worms experimentally exposed to the presence or absence of sperm competition did not differ in sex allocation, sperm transfer or mating behavior. A response to our manipulation would have required individual recognition, the ability to detect self-referencing tags, or tags or traces left by rivals on or in the mating partners. We first discuss the possibility that highly efficient sperm displacement may have decreased the difference between the treatment groups and then propose three alternative cues that may allow M. lignano to respond plastically to the social group size manipulation used in earlier studies: assessment of the mating rate, chemical cues, or tactile cues. [source]

The Bold and the Variable: Fish with High Heterozygosity Act Recklessly in the Vicinity of Predators

ETHOLOGY, Issue 1 2008
Sampsa Vilhunen
Variation in the innate behavioral response to predation threat is often assumed to reflect genetic differences among the prey individuals. To date, no published results, however, exist that would offer explanation for the origin of this behavioral variation within populations. Using microsatellites as markers, we estimated the genetic variability of juvenile brown trout (Salmo trutta) individuals whose behavior had been individually recorded in a trade-off situation where both predator chemical cues and food were present. Mean overall heterozygosity and the internal relatedness of fish associated significantly with their activity and foraging, so that the genetically more variable individuals showed more risk-prone behavior under predation risk. No association between genetic variability and behavior was found in trials where predator odors were not present. These results were consistent over the three study populations of brown trout with different backgrounds, suggesting that the phenomenon is of general nature in this species. Of the possible mechanisms suggested to enable the existence of the positive association between neutral microsatellite variation and fitness-related trait, the local effect hypothesis gained more support from our data than the general effect hypothesis. [source]

The Influence of Flowing Water on the Resource Pursuit-Risk Avoidance Tradeoff in the Crayfish Orconectes virilis

ETHOLOGY, Issue 4 2006
Keith W. Pecor
The influence of hydrodynamics on chemically mediated behavioral tradeoffs has received little attention. We tested the hypothesis that individuals of the crayfish Orconectes virilis would be more sensitive to chemical cues in flowing water than in still water. Orconectes virilis is a good subject for this test, because it is found in both still water (e.g. ponds), and flowing water (e.g. rivers). A factorial design was used, with two stimulus treatments and two habitat types. Crayfish were exposed to either food cue or food + alarm cue in either still water or flowing water in an artificial stream arena. Habitat use and activity were significantly influenced by stimulus treatment, with more time spent away from the stimulus source and less activity in the food + alarm treatment than in the food treatment. Neither habitat type nor the interaction of stimulus treatment and habitat type had a significant effect on the response variables. Given the natural history of O. virilis, we suggest that selection has favored the ability to equally utilize chemical cues in both still and flowing water. We acknowledge that different flow conditions may influence chemical ecology in this species and caution against the view that tests in flowing waters necessarily provide a more accurate approximation of natural responses. [source]

Predator Inspection Behaviour in a Characin Fish: an Interaction between Chemical and Visual Information?

ETHOLOGY, Issue 9 2003
Grant E. Brown
Recent evidence suggests that predator inspection behaviour by Ostariophysan prey fishes is regulated by both the chemical and visual cues of potential predators. In laboratory trials, we assessed the relative importance of chemical and visual information during inspection visits by varying both ambient light (visual cues) and predator odour (chemical cues) in a 2 × 2 experimental design. Shoals of glowlight tetras (Hemigrammus erythrozonus) were exposed to a live convict cichlid (Archocentrus nigrofasciatus) predator under low (3 lux) or high (50 lux) light levels and in the presence of the odour of a cichild fed tetras (with an alarm cue) or swordtails (Xiphophorus helleri, with an alarm cue not recognized by tetras). Tetras exhibited threat-sensitive inspection behaviour (increased latency to inspect, reduced frequency of inspection, smaller inspecting group sizes and increased minimum approach distance) towards a predator paired with a tetra-fed diet cue, regardless of light levels. Similar threat-sensitive inspection patterns were observed towards cichlids paired with a swordtail-fed diet cue only under high light conditions. Our data suggest that chemical cues in the form of prey alarm cues in the diet of the predator, are the primary source of information regarding local predation risk during inspection behaviour, and that visual cues are used when chemical information is unavailable or ambiguous. [source]

Population and Species Divergence of Chemical Cues that Influence Male Recognition of Females in Desmognathine Salamanders

Responses of Snow Voles, Chionomys nivalis, Towards Conspecific Cues Reflect Social Organization during Overwintering Periods

ETHOLOGY, Issue 11 2002
Juan J. Luque-Larena
Among microtine rodents, reaction to chemical cues from conspecifics is assumed to reflect social and spatial relationships. Generally, strong attraction of particular odours correlates with non-aggressive behaviour and high spatial tolerance towards odour donors, whereas weak attraction correlates with greater levels of aggression and spatial segregation. In the present study, we examined whether winter odour preferences of the snow vole Chionomys nivalis, a rock-dwelling microtine principally found at high-mountainous regions, differ from that of other vole species, owing to their different social organization during overwintering periods. The social structure of C. nivalis over the winter period is relatively unusual among vole species in that they become nomadic and solitary. In odour choice trials under laboratory conditions, we found that both males and females avoided zones with conspecific odours of both sexes in comparison with unscented control zones or own odours. These results are consistent with the elevated levels of intraspecific aggression and spatial isolation of C. nivalis during overwintering periods. Furthermore, scent-elicited self-grooming increased when their own odour was offered against conspecific cues. This, in combination with an active avoidance of conspecific odours, might functionally contribute to minimize direct confrontations between solitary individuals, thereby reducing the risks of aggressive encounters during overwintering periods. [source]

Sexual Dimorphism of Mate Location in the Long-Toed Salamander Ambystoma macrodactylum columbianum

ETHOLOGY, Issue 8 2001
Paul Verrell
In breeding systems characterized by scramble competition among males, theory predicts that the efficient location of mating partners is more important to males than to females as a component of mating success. We experimentally tested in the laboratory the hypothesis that breeding male long-toed salamanders (Ambystomamacrodactylumcolumbianum), which scramble for mating opportunities, are better able to recognize and locate potential mates than are breeding females. Males were more likely to enter traps containing females than empty traps or traps containing males. Traps containing sponges soiled by females were more likely to attract males than traps containing clean (control) sponges, suggesting that chemical cues may be sufficient for mate location by males. Females were no more likely to enter traps containing males than empty traps. Our results are consistent with the theoretical prediction that selection has been stronger on male long-toed salamanders than on females in the context of capacity for recognizing and locating potential mating partners. [source]

Learned Recognition of Intraspecific Predators in Larval Long-Toed Salamanders Ambystoma macrodactylum

ETHOLOGY, Issue 6 2001
Erica L. Wildy
The ability of prey to detect predators and respond accordingly is critical to their survival. The use of chemical cues by animals in predator detection has been widely documented. In many cases, predator recognition is facilitated by the release of alarm cues from conspecific victims. Alarm cues elicit anti-predator behavior in many species, which can reduce their risk of being attacked. It has been previously demonstrated that adult long-toed salamanders, Ambystoma macrodactylum, exhibit an alarm response to chemical cues from injured conspecifics. However, whether this response exists in the larval stage of this species and whether it is an innate or a learned condition is unknown. In the current study, we examined the alarm response of naïve (i.e. lab-reared) larval long-toed salamanders. We conducted a series of behavioral trials during which we quantified the level of activity and spatial avoidance of hungry and satiated focal larvae to water conditioned by an injured conspecific, a cannibal that had recently been fed a conspecific or a non-cannibal that was recently fed a diet of Tubifex worms. Focal larvae neither reduced their activity nor spatially avoided the area of the stimulus in either treatment when satiated, and exhibited increased activity towards the cannibal stimulus when hungry. We regard this latter behavior as a feeding response. Together these results suggest that an anti-predator response to injured conspecifics and to cannibalistic conspecifics is absent in naïve larvae. Previous studies have shown that experienced wild captured salamanders do show a response to cannibalistic conspecifics. Therefore, we conducted an additional experiment examining whether larvae can learn to exhibit anti-predator behavior in response to cues from cannibalized conspecifics. We exposed larvae to visual, chemical and tactile cues of stimulus animals that were actively foraging on conspecifics (experienced) or a diet of Tubifex (naïve treatment). In subsequent behavioral treatments, experienced larvae significantly reduced their activity compared to naive larvae in response to chemical cues of cannibals that had recently consumed conspecifics. We suggest that this behavior is a response to alarm cues released by consumed conspecifics that may have labeled the cannibal. Furthermore, over time, interactions with cannibals may cause potential prey larvae to learn to avoid cannibals regardless of their recent diet. [source]

Non-lethal effects of invertebrate predators on Daphnia: morphological and life-history consequences of water mite kairomone

FRESHWATER BIOLOGY, Issue 9 2008
MAURICIO J. CARTER
Summary 1. Here, we report morphological and life-historical changes in the cladoceran Daphnia ambigua in response to chemical cues released by the predatory water mite Piona chilensis. Both species are common inhabitants of southern temperate lakes. 2. We found significant differences in adult body size at first, second and third reproduction. Also, individuals exposed to kairomones had longer tail spines at first reproduction, and the resultant offspring had smaller bodies and shorter tail spines. 3. Exposure to mite cues did not exert effects on brood size at first reproduction, but decreased offspring number in subsequent broods. Similarly, only the second and third reproduction events were delayed by kairomone exposure. 4. The intrinsic population growth rate of predator-induced animals was lower than that in controls, but simulations based on a parameterized matrix model showed that the fitness costs could be overcome if the reported phenotypic responses reduced predation rate moderately. The gain in protection from predators needed to cancel out the reduction in fitness associated with predator cues was directly related to juvenile survival and fertility, and inversely related to adult survival. 5. This is the first work reporting phenotypic plasticity in Cladocera in response to kairomones released by water mites, which are conspicuous predators in many austral fresh waters. [source]

Lethal and non-lethal effects of multiple indigenous predators on the invasive golden apple snail (Pomacea canaliculata)

FRESHWATER BIOLOGY, Issue 10 2004
Nils Carlsson
Summary 1. We investigated the individual and combined effects of two predators (the climbing perch, Anabas testudineus, and the wetland crab, Esanthelphusa nimoafi) indigenous to wetlands in Laos, on the behaviour and survival of the invasive South American golden apple snail (Pomacea canaliculata). The snail is considered a pest, consuming large amounts of rice and other aquatic vegetation in the region. 2. Snail avoidance reactions to released predator chemical cues were investigated in aquaria while the effects of predators on a mixed snail population were studied in field enclosures that contained native aquatic plants (Salvinia cucullata, Ludwigia adscendens and Ipomoea aquatica). 3. In the aquaria experiment, neonate (2,3 mm) and medium-sized snails (8,10 mm) responded to fish chemical cues by going to the surface, whereas adult snails (35,40 mm) went to the bottom. In contrast, no size class of snails reacted to chemical cues released by crabs. 4. In the field experiment, fish reduced the abundance of neonate snails, and crabs reduced the abundance of all size classes. The effect of the combined predators could not be predicted from the mortality rate observed in single predator treatments. The survival of neonate and medium-sized snails was greater and of adults less than expected. The presence of predators did not affect egg production. Snails consumed significant amounts of plants despite the presence of predators. 5. Our findings suggest that some indigenous Asian predators have lethal and sublethal effects on P. canaliculata that depend on snail size and predator type. When in the presence of several predators the response of snails to one predator may either increase or decrease the vulnerability of snails to the others. [source]

The influence of predatory fish on mayfly drift: extrapolating from experiments to nature

FRESHWATER BIOLOGY, Issue 8 2002
ANGUS R. MCINTOSH
1.,A knowledge of how individual behaviour affects populations in nature is needed to understand many ecologically important processes, such as the dispersal of larval insects in streams. The influence of chemical cues from drift-feeding fish on the drift dispersal of mayflies has been documented in small experimental channels (i.e. < 3 m), but their influence on dispersal in natural systems (e.g. 30 m stream reaches) is unclear. 2.,Using surveys in 10 Rocky Mountain streams in Western Colorado we examined whether the effects of predatory brook trout (Salvelinus fontinalis) on mayfly drift, that were apparent in stream-side channels, could also be detected in natural streams. 3.,In channel experiments, the drift of Baetis bicaudatus (Baetidae) was more responsive to variation in the concentration of chemical cues from brook trout than that of another mayfly, Epeorus deceptivus (Heptageniidae). The rate of brook trout predation on drifting mayflies of both species in a 2-m long observation tank was higher during the day (60,75%) but still measurable at night (5,10%). Epeorus individuals released into the water column were more vulnerable to trout predation by both day and night than were Baetis larvae treated similarly. 4.,Drift of all mayfly taxa in five fishless streams was aperiodic, whereas their drift was nocturnal in five trout streams. The propensity of mayflies to drift was decreased during the day and increased during the night in trout streams compared with fishless streams. In contrast to the channel experiments, fish biomass and density did not alter the nocturnal nature nor magnitude of mayfly drift in natural streams. 5.,In combination, these results indicate that mayflies respond to subtle differences in concentration of fish cues in experimental channels. However, temporal and spatial variation in fish cues available to mayflies in natural streams may have obscured our ability to detect responses at larger scales. [source]

Multiple predator-avoidance behaviours of the freshwater snail Physella heterostropha pomila: responses vary with risk

FRESHWATER BIOLOGY, Issue 3 2000
Thomas M. McCarthy
Summary 1We examined the predator-avoidance behaviour, exhibited in response to chemical cues, of two populations of the snail Physella heterostropha pomila. Snails were subjected to four treatments simulating different degrees of predation risk: control water (low risk), or water from tanks containing nonforaging crayfish (intermediate risk), crushed conspecifics (high risk) or crayfish consuming conspecifics (high risk). Data were analysed using three-way ANOVA models (population × predator chemicals × injured conspecific chemicals). 2Physella increased its avoidance behaviour as risk increased. Crayfish cue elicited a significantly greater response than from controls. Cues from injured conspecifics elicited the strongest response. 3Physella exhibited several types of avoidance behaviour, including burial into the substratum, moving to the water surface, and crawling out of the water. The type of cue present influenced response type. Cues from crayfish reduced burial and increased movement to the water surface or out of the water. Cues from injured-conspecifics significantly increased crawling completely out of the water. 4The two populations differed in the type and degree of response exhibited. One population exhibited significantly greater ,reactivity' (i.e. any avoidance behaviour) in response to foraging crayfish, and more burial and crawl-out behaviours were exhibited in high-risk treatments. [source]

Interpreting the smells of predation: how alarm cues and kairomones induce different prey defences

FUNCTIONAL ECOLOGY, Issue 6 2009
Nancy M. Schoeppner
Summary 1.,For phenotypically plastic organisms to produce phenotypes that are well matched to their environment, they must acquire information about their environment. For inducible defences, cues from damaged prey and cues from predators both have the potential to provide important information, yet we know little about the relative importance of these separate sources of information for behavioural and morphological defences. We also do not know the point during a predation event at which kairomones are produced, i.e. whether they are produced constitutively, during prey attack or during prey digestion. 2.,We exposed leopard frog tadpoles (Rana pipiens) to nine predator cue treatments involving several combinations of cues from damaged conspecifics or heterospecifics, starved predators, predators only chewing prey, predators only digesting prey or predators chewing and digesting prey. 3.,We quantified two behavioural defences. Tadpole hiding behaviour was induced only by cues from crushed tadpoles. Reduced tadpole activity was induced only by cues from predators digesting tadpoles or predators chewing + digesting tadpoles. 4.,We also quantified tadpole mass and two size-adjusted morphological traits that are known to be phenotypically plastic. Mass was unaffected by the cue treatments. Relative body length was affected (i.e. there were differences among some treatments), but none of the treatments significantly differed from the no-predator control. Relative tail depth was affected by the treatments and deeper tails were induced only when tadpoles were exposed to cues from predators digesting tadpoles or cues from predators chewing + digesting tadpoles. 5.,These results demonstrate that some prey species can discriminate among a diverse set of potential cues from heterospecific prey, conspecific prey and predators. Moreover, the results illustrate that the cues responsible for the full suite of behavioural and morphological defences are not induced by tadpole crushing nor can they be induced by generalized digestive chemicals produced when predators digest their prey. Instead, both prey damage and predator digestion of conspecific tissues appear to be important for communicating predatory risk to phenotypically plastic anuran prey. Importantly, the production of chemical cues by predators may be unavoidable and prey have evolved the ability to eavesdrop on these signals. [source]

Functionalized, Swellable Hydrogel Layers as a Platform for Cell Studies

ADVANCED FUNCTIONAL MATERIALS, Issue 8 2009
Núria Marí-Buyé
Abstract This paper reports the design, synthesis and characterization of thin films as a platform for studying the separate influences of physical and chemical cues of a matrix on the adhesion, growth and final phenotype of cells. Independent control of the physical and chemical properties of functionalized, swellable hydrogel thin films is achieved using initiated chemical vapor deposition (iCVD). The systematic variation in crosslink density is demonstrated to control the swelling ability of the iCVD hydrogel films based on 2-hydroxyethyl methacrylate (HEMA). At the same time, the incorporation of controllable concentrations of the active ester pentafluorophenyl methacrylate (PFM) allows easy immobilization of aminated bioactive motifs, such as bioactive peptides. Initial cell culture results with human umbilical vein endothelial cells (HUVEC) indicate that the strategy of using PFM to immobilize a cell-adhesion peptide motif onto the hydrogel layers promotes proper HUVEC growth and enhances their phenotype. [source]

Tissue Engineering Strategies Designed to Realize the Endogenous Regenerative Potential of Peripheral Nerves

ADVANCED MATERIALS, Issue 46 2009
Vivek Mukhatyar
Abstract http://doi.wiley.com/10.1002/adma.v21:32/33 Bridging peripheral nerve gaps without the use of autografts has significant clinical importance. But in order to rationally design novel scaffolds, a good understanding of the nerve regeneration process is vital. Appropriate amount of structural and chemical cues are required to stimulate the endogenous mechanisms of repair and functional recovery. Synthetic and natural materials present various opportunities to induce the growth of supporting cells as well as promote axon regeneration. An overview of tissue engineering strategies currently being explored that stimulate the different steps of the regenerative sequence is presented. [source]

Is the (E)- , -farnesene only volatile terpenoid in aphids?

JOURNAL OF APPLIED ENTOMOLOGY, Issue 1 2005
F. Francis
Abstract:, Herbivore insects use a broad range of chemical cues to locate their host to feed or to oviposit. Whether several plant volatiles are effective allelochemicals for insects, the latter also emit molecules which have infochemical role. The (E)- , -farnesene (EBF) is a well-known aphid alarm pheromone commonly found in all previously tested species. Analysis of the released molecules from 23 aphid species, mainly collected on their natural host plant from May to July, was performed by gas chromatography,mass spectrometry. While EBF was identified as the main volatile substance in 16 species, alone or associated with other molecules, the alarm pheromone was only a minor component of the volatile molecule pattern of five other species. Moreover, two species, Euceraphis punctipennis and Drepanosiphum platanoides, did not release EBF at all but other terpenes were identified. This original observation raised the question on the utility and the source of the non-EBF volatiles. Are these potential infochemical substances produced by the aphid or only absorbed from the host plant? Here we determined that terpenes released by insects were not only provided by the host plants. Indeed, Megoura viciae emitted additional molecules than the ones from several aphid species reared on the same host plant. Moreover, no systematic relation between the feeding behaviour of the aphid species and the volatile releases was observed. Aphid terpene composition and proportion would provide reliable cues to identify the emitting organism, plant or insect. The next step of this work will be to determine the infochemical role of terpenes found in the range of tested aphid samples to better understand the relations between the different tritrophic levels. [source]

Eastern bluebirds Sialia sialis do not avoid nest boxes with chemical cues from two common nest predators

JOURNAL OF AVIAN BIOLOGY, Issue 1 2007
Renee D. Godard
Chemodetection of common nest predators may be advantageous for nesting birds; however, few studies have examined the ability of songbirds to detect chemical odors from predators. Thus, in 2002, we presented eastern bluebirds Sialia sialis with pairs of nest boxes; one box in the pair was regularly scented with chemical cues from a common nest predator, the black rat snake Elaphe obsoleta, and the other with a neutral cue. In 2004, we again presented bluebirds with pairs of boxes, one scented with chemical cues from a different nest predator, the deer mouse Peromyscus maniculatus, and the other with a neutral scent. Although human females were able to correctly distinguish paper laced with predator cues from paper with neutral cues, bluebirds were as likely to lay eggs in boxes with predator cues as in boxes with neutral cues. While it remains possible that bluebirds may detect scent from potential nest predators, it appears that the presence of these chemical cues does not ultimately influence selection of nest sites. [source]

Attraction of slimy sculpins to chemical cues of brook charr eggs

JOURNAL OF FISH BIOLOGY, Issue 3 2002
R. S. Mirza
In a laboratory study to examine the responses of slimy sculpin Cottus cognatus to chemical cues of brook charr Salvelinus fontinalis eggs, water that held freshly laid eggs (<20 min old), water-hardened eggs (i.e. eggs >4 h old) or injured eggs was collected and used in a series of two-choice tests. Slimy sculpin were exposed to paired stimuli of (1) hard egg water v. control water, (2) fresh egg water v. control water, (3) hard egg water v. injured egg water or (4) hard egg water v. fresh egg water. Sculpin spent considerably more time on the side of the tank with hard egg water and fresh egg water v. control water and injured egg water v. hard egg water. Sculpin did not show a preference for hard egg water v. fresh egg water. In a field study, brook charr were attracted to chemical cues from brook charr eggs, suggesting that brook charr eggs produce sufficient odour to attract some species under natural conditions. [source]

A review of host finding behaviour in the parasitic sea louse, Lepeophtheirus salmonis (Caligidae: Copepoda)

JOURNAL OF FISH DISEASES, Issue 1 2009
A J Mordue (Luntz)
Abstract Ectoparasitic sea lice are the most important parasite problem to date for the salmon farming industry in the northern and southern hemispheres. An understanding of host location in the specialist species, Lepeophtheirus salmonis, the most important louse species in the North Atlantic, is now being realized using behavioural in vitro and in vivo bioassays coupled with chemical analysis of fish conditioned waters. Both physical and chemical cues are important in host location. Responses of sea lice to physical cues such as light and salinity may enable them to gather in areas where host fish are likely to be found. Mechanoreception is an important sensory modality in host location and acts by switching on specific behaviours that enable landing on a fish. Chemoreception plays a defining role in host location and recognition. The detection of host kairomones switches on ,host search' behavioural patterns and also induces landing responses whereas non-host kairomones fail to induce attraction or significant landing behaviour. Semiochemicals derived from salmon and also non-host fish have been identified, and may prove useful for the development of integrated pest management strategies, by the introduction of odour traps for monitoring lice numbers, and by the use of stimulo-deterrent diversionary (push:pull) strategies in their control. [source]

Urinary chemosignals in giant pandas (Ailuropoda melanoleuca): seasonal and developmental effects on signal discrimination

JOURNAL OF ZOOLOGY, Issue 3 2004
A. M. White
Abstract As a solitary species, giant pandas Ailuropoda melanoleuca have a chemical communication system that allows for avoidance with conspecifics throughout most of the year and facilitates breeding during their brief reproductive period. To date, most studies have focused on adults during the breeding season, but much remains to be learned about developmental and seasonal effects on chemical communication. Here, using odour discrimination methodology, we examine these contextual influences on chemosignalling in giant pandas for the first time. During the breeding season both adult and subadult giant pandas overtly discriminated conspecific sex using chemical cues in urine. Male urine was consistently investigated more than female urine by pandas across all age-sex categories. This preference for male urine was observed for both adult and subadult urine donors, indicating that adult levels of reproductive hormones are not necessary for the production of sex-specific urinary odour cues. By contrast, giant pandas did not overtly discriminate sex during the non-breeding season. This finding underscores that negative results for discrimination studies need to be interpreted with caution, as many contextual factors may influence overt expression of this ability. Evidence points to seasonal changes in motivation, rather than in the availability of sex-specific chemical cues in urine, as the causal factor for changing odour discrimination patterns. These and other findings are discussed in the light of the potential role that discrimination of odour plays in the regulation of competitive and reproductive interactions in nature. [source]

Changes in survivorship, behavior, and morphology in native soft-shell clams induced by invasive green crab predators

MARINE ECOLOGY, Issue 3 2010
W. Lindsay Whitlow
Abstract Many studies on invasive species show reduced native densities, but few studies measure trait-mediated effects as mechanisms for changes in native growth rates and population dynamics. Where native prey face invasive predators, mechanisms for phenotypic change include selective predation, or induced behavioral or morphological plasticity. Invasive green crabs, Carcinus maenas, have contributed to declines in native soft-shell clams, Mya arenaria, in coastal New England, USA. We tested the hypothesis that clam ability to detect chemical cues from predators or damaged conspecifics would induce greater burrowing depth as a refuge from invasive crabs, and greater burrowing would require increased siphon growth. To determine how crab predation affected clam survivorship and phenotypic traits in the field, clams in exclosure, open, and crab enclosure plots were compared. Crab predation reduced clam density, and surviving clams were deeper and larger, with longer siphons. To determine whether the mechanism for these results was selective predation or induced plasticity, phenotypes were compared between clams exposed to chemical cues from crab predation and clams exposed to seawater in laboratory and field experiments. In response to crab predation cues, clams burrowed deeper, with longer siphons and greater siphon mass. Overall, crab predation removed clams with shorter siphons at shallow depths, and crab predation cues induced greater burrowing depths and longer siphons. Longer siphons and greater siphon mass of deeper clams suggests clams may allocate energy to siphon growth in response to crabs. By determining native behavior and morphological changes in response to an invasive predator, this study adds to our understanding of mechanisms for invasive impacts and illustrates the utility of measuring trait-mediated effects to investigate predator,prey dynamics. [source]