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Chain Reaction System (chain + reaction_system)

Distribution by Scientific Domains
Life Sciences50%
Chemistry50%

Kinds of Chain Reaction System

  • polymerase chain reaction system
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    Selected Abstracts

    Automation of DNA marker analysis for molecular breeding in crops: practical experience of a plant breeding company

    PLANT BREEDING, Issue 4 2007
    C. Dayteg
    Abstract In modern plant breeding, DNA marker analyses are of increasing importance and, as the methods become more widely adopted, the capacity for high-throughput analyses at low cost is crucial for its practical use. Automation of the analysis processes is a way to meet these requirements. In order to achieve this, while keeping adequate flexibility in the analysis processes, Svalöf Weibull AB (SW) has developed a fully automated polymerase chain reaction system. It has been evaluated on barley and canola lines and is capable of analysing up to 2200 samples per day at a cost of 0,24 , per analysis for marker-assisted selection and quality control of genetically modified organisms. A detailed description of this system is given, and improvements to the throughput and applications are discussed. [source]

    Matrix-assisted laser desorption/ionization detection of polymerase chain reaction products by utilizing the 5,-3, exonuclease activity of Thermus aquaticus DNA polymerase,

    RAPID COMMUNICATIONS IN MASS SPECTROMETRY, Issue 6 2003
    N. R. Isola
    The 5,-3, exonuclease activity of DNA polymerase was utilized in the polymerase chain reaction system to generate a specific signal concomitant with amplification. These signals were detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). This method obviates the need to perform extensive DNA purification of reaction products that is often necessary for detecting larger DNA molecules by mass spectrometry. Oligonucleotides complementary to the internal region of the amplicon are degraded by the 5,-3, exonuclease activity and the degradation products are analyzed by MALDI mass spectrometry. We refer to this assay as the Exo-taq assay or probe degradation assay. This method should be amenable to automation. Copyright © 2003 John Wiley & Sons, Ltd. [source]

    Development and design of a ,ready-to-use' reaction plate for a PCR-based simultaneous detection of animal species used in foods

    INTERNATIONAL JOURNAL OF FOOD SCIENCE & TECHNOLOGY, Issue 1 2007
    Ines Laube
    Summary Different TaqManTM -polymerase chain reaction systems have been developed, which allow the detection of even minute amounts of beef, pork, lamb, goat, chicken, turkey and duck in processed foods. The species-specific systems are able to amplify DNA regions with no more than 108 bp in size (exception: duck, 212 bp) located on the single-copy genes cyclic guanosine monophosphate (cyclic GMP) phosphodiesterase, ryanodine receptor and interleukin -2 precursor. The parallel detection of the common ingredient ,meat' produced from mammals and poultry was based on the amplification of a region of the myostatin gene. The limit of detection was determined to be ten genome copies for each system. The relative SD under repeatability condition was below 30%. In addition, a ,ready-to-use' reaction plate has been developed, which makes it possible to investigate the presence of the seven animal species in parallel after a single real-time run. [source]

    INVITED REVIEW: Molecular analysis of predation: a review of best practice for DNA-based approaches

    MOLECULAR ECOLOGY, Issue 4 2008
    R. A. KING
    Abstract Molecular analysis of predation, through polymerase chain reaction amplification of prey remains within the faeces or digestive systems of predators, is a rapidly growing field, impeded by a lack of readily accessible advice on best practice. Here, we review the techniques used to date and provide guidelines accessible to those new to this field or from a different molecular biology background. Optimization begins with field collection, sample preservation, predator dissection and DNA extraction techniques, all designed to ensure good quality, uncontaminated DNA from semidigested samples. The advantages of nuclear vs. mitochondrial DNA as primer targets are reviewed, along with choice of genes and advice on primer design to maximize specificity and detection periods following ingestion of the prey by the predators. Primer and assay optimization are discussed, including cross-amplification tests and calibratory feeding experiments. Once primers have been made, the screening of field samples must guard against (through appropriate controls) cross contamination. Multiplex polymerase chain reactions provide a means of screening for many different species simultaneously. We discuss visualization of amplicons on gels, with and without incorporation of fluorescent primers. In more specialized areas, we examine the utility of temperature and denaturing gradient gel electrophoresis to examine responses of predators to prey diversity, and review the potential of quantitative polymerase chain reaction systems to quantify predation. Alternative routes by which prey DNA might get into the guts of a predator (scavenging, secondary predation) are highlighted. We look ahead to new technologies, including microarrays and pyrosequencing, which might one day be applied to this field. [source]