Chronological Lifespan (chronological + lifespan)

Distribution by Scientific Domains


Selected Abstracts


Chronological and replicative lifespan of polyploid Saccharomyces cerevisiae (syn. S. pastorianus)

FEMS YEAST RESEARCH, Issue 2 2003
Dawn L Maskell
Abstract Chronological lifespan may be defined as the result of accumulation of irreversible damage to intracellular components during extended stationary phase, compromising cellular integrity and leading to death and autolysis. In contrast, replicative lifespan relates to the number of divisions an individual cell has undertaken before entering a non-replicative state termed senescence, leading to cell death and autolysis. Both forms of lifespan have been considered to represent models of ageing in higher eukaryotes, yet the relation between chronologically and replicatively aged populations has not been investigated. In this study both forms of lifespan have been investigated in Saccharomyces cerevisiae (Syn. S. pastorianus) to establish the relationship between chronological and replicative ageing. [source]


A novel gene, ecl1+, extends the chronological lifespan in fission yeast

FEMS YEAST RESEARCH, Issue 4 2008
Hokuto Ohtsuka
Abstract We have identified a novel gene from Schizosaccharomyces pombe that we have named ecl1+ (extender of the chronological lifespan). When ecl1+ is provided on a high-copy number plasmid, it extends the viability of both the ,sty1 MAP kinase mutant and the wild-type cells after entry into the stationary phase. ecl1+ encodes an 80-amino acid polypeptide that had not been annotated in the current database. The ecl1+ -mRNA increases transiently when the growth phase is changed from the log phase to the stationary phase. The Ecl1 protein is localized in the nucleus. Calorie restriction extends the chronological lifespan of wild-type and ,ecl1 cells but not ecl1+ -overproducing cells. The ,pka1 mutant shows little, if any, additional extension of viability when Ecl1 is overproduced. The ste11+ gene that is negatively controlled by Pka1 is up regulated when Ecl1 is overproduced. From these results we propose that the effect of Ecl1 overproduction may be mainly linked to and negatively affects the Pka1-dependent pathway. [source]


Autophagy and amino acid homeostasis are required for chronological longevity in Saccharomyces cerevisiae

AGING CELL, Issue 4 2009
Ashley L. Alvers
Summary Following cessation of growth, yeast cells remain viable in a nondividing state for a period of time known as the chronological lifespan (CLS). Autophagy is a degradative process responsible for amino acid recycling in response to nitrogen starvation and amino acid limitation. We have investigated the role of autophagy during chronological aging of yeast grown in glucose minimal media containing different supplemental essential and nonessential amino acids. Deletion of ATG1 or ATG7, both of which are required for autophagy, reduced CLS, whereas deletion of ATG11, which is required for selective targeting of cellular components to the vacuole for degradation, did not reduce CLS. The nonessential amino acids isoleucine and valine, and the essential amino acid leucine, extended CLS in autophagy-deficient as well as autophagy-competent yeast. This extension was suppressed by constitutive expression of GCN4, which encodes a transcriptional regulator of general amino acid control (GAAC). Consistent with this, GCN4 expression was reduced by isoleucine and valine. Furthermore, elimination of the leucine requirement extended CLS and prevented the effects of constitutive expression of GCN4. Interestingly, deletion of LEU3, a GAAC target gene encoding a transcriptional regulator of branched side chain amino acid synthesis, dramatically increased CLS in the absence of amino acid supplements. In general, this indicates that activation of GAAC reduces CLS whereas suppression of GAAC extends CLS in minimal medium. These findings demonstrate important roles for autophagy and amino acid homeostasis in determining CLS in yeast. [source]


NQR1 controls lifespan by regulating the promotion of respiratory metabolism in yeast

AGING CELL, Issue 2 2009
María Jiménez-Hidalgo
Summary The activity and expression of plasma membrane NADH coenzyme Q reductase is increased by calorie restriction (CR) in rodents. Although this effect is well-established and is necessary for CR's ability to delay aging, the mechanism is unknown. Here we show that the Saccharomyces cerevisiae homolog, NADH-Coenzyme Q reductase 1 (NQR1), resides at the plasma membrane and when overexpressed extends both replicative and chronological lifespan. We show that NQR1 extends replicative lifespan in a SIR2-dependent manner by shifting cells towards respiratory metabolism. Chronological lifespan extension, in contrast, occurs via an SIR2-independent decrease in ethanol production. We conclude that NQR1 is a key mediator of lifespan extension by CR through its effects on yeast metabolism and discuss how these findings could suggest a function for this protein in lifespan extension in mammals. [source]


The impact of media composition and petite mutation on the longevity of a polyploid brewing yeast strain

LETTERS IN APPLIED MICROBIOLOGY, Issue 1 2000
C.D. Powell
Ageing in Saccharomyces cerevisiae is a finite phenomenon, determined by replicative, rather than chronological lifespan. Yeast physiological condition is known to influence industrial fermentation performance, however, until recently cellular senescence has not been considered as a brewing yeast stress factor. A polyploid lager yeast (BB11) and a brewery isolate, exhibiting petite mutation were analysed for longevity. It was observed that mitochondrial deficiency induced a reduction in lifespan. In addition, replicative capacity was perceived to be dependent on environmental conditions. [source]