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Chromosomal Polymorphism (chromosomal + polymorphism)
Selected AbstractsChromosomal Polymorphism in Korean Natural Populations of Drosophila immigransENTOMOLOGICAL RESEARCH, Issue 1 2003Nam Woo KIM ABSTRACT To analyze chromosome inversions of Drosophila immigrans, wild flies were captured from large vineyards located in the suburbs of Yecheon and Gyeongsan from October 1999 to 2001. With the egg samples obtained singly at each of the 799 females of D. immigrans, cytological examinations were carried out for the type and frequency of inversions. Two types of different inversions were found only in the second chromosome. The inversions detected were known to be the cosmopolitan inversion "A" and "B". The mean frequency of inversion A was estimated to be 0.074 in Yecheon and 0.066 in Gyeongsan and that of B was to be 0.026 in Yecheon and 0.021 in Gyeongsan, respectively. In the frequency ratio, inversion A was significantly higher than that of B. The present populations of D. immigrans showed subtle differences from other Korean populations in inversion frequencies. To account for the local variations observed in inversion frequencies, several hypotheses are discussed such as founding event or selective force. [source] Reproductive isolation between chromosomal races of the house mouse Mus musculus domesticus in a parapatric contact area revealed by an analysis of multiple unlinked lociJOURNAL OF EVOLUTIONARY BIOLOGY, Issue 2 2008P. FRANCHINI Abstract The house mouse, Mus musculus domesticus, exhibits a high level of chromosomal polymorphism because of the occurrence and fast fixation of Robertsonian fusions between telocentric chromosomes. For this reason, it has been considered a classical speciation model to analyse the role of the chromosomal changes in reproductive isolation. In this study, we analysed a parapatric contact area between two metacentric races in central Italy, the Cittaducale race (CD: 2n = 22) and the Ancarano race (ACR: 2n = 24), to estimate gene flow at the boundary. Hybrids between these two races show high levels of structural heterozygosity and are expected to be highly infertile. A sample of 88 mice from 14 sites was used. The mice were genotyped by means of eight microsatellite loci mapped in four different autosomal arms. The results show clear genetic differentiation between the CD and ACR races, as revealed by differences in allele frequencies, factorial correspondence analysis and indexes of genetic population (e.g. FST and RST) along the contact zone. The genetic differentiation between the races was further highlighted by assignation and clustering analyses, in which all the individuals were correctly assigned by their genotypes to the source chromosomal race. This result is particularly interesting in view of the absence of any geographical or ecological barrier in the parapatric contact zone, which occurs within a village. In these conditions, the observed genetic separation suggests an absence of gene flow between the races. The CD,ACR contact area is a rare example of a final stage of speciation between chromosomal races of rodents because of their chromosomal incompatibility. [source] Long-term changes in the chromosomal inversion polymorphism of Drosophila subobscura.JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, Issue 3 2004Abstract The chromosomal polymorphism of 13 European populations of Drosophila subobscura has been compared with that of the same populations collected 15,35 years ago. The chromosomal polymorphism of the old populations differs significantly from that of the new populations, mainly for chromosomes U and O. There is a very good agreement between the geographical space and the genetic space as shown by a graphical representation of the 26 statistical populations (13 old and 13 new) obtained by a principal coordinate analysis. This reflects both the existence of significant latitudinal clines for the frequencies of some chromosomal arrangements in the old and new samples and systematic changes that have taken place in these populations during the period that elapsed between the two surveys. An increase in the frequency of those arrangements typical of southern latitudes and a decrease for those common in northern latitudes is observed in all populations , Mediterranean, Atlantic and Central European. Furthermore, the genetic distances of the new populations to a southern population of reference have decreased in comparison with those of the old populations. These changes could be the result of climatic factors that are correlated with latitude. In particular, the assumption that global warming is responsible for all the changes observed appears rather likely. Whether these systematic changes of the chromosomal polymorphism are a consequence of local adaptations or have been produced by migration from the south remains an open question. Zusamennfassung Der gegenwärtige chromosomale Inversionspolymorphismus in 13 europäischen Populationen von Drosophila subobscura wurde mit dem Zustand in denselben Populationen verglichen, die vor 15 bis 35 Jahren untersucht worden waren. Der Chromosomenpolymorphismus der ,,alten'' Populationen unterscheidet sich signifikant von dem der ,,neuen'' Populationen, besonders für die Chromosomen U und O. Eine sehr gute Übereinstimmung zeigt sich zwischen der geographischen Komponente und der genetischen Komponente bei einer graphischen Darstellung der 26 Populationen (13 alte und 13 neue Populationen) durch eine Koordinatenanalyse. Das spiegelt beides wider, die Existenz von signifikanten breitenabhängigen Klinen in der Häufigkeit einiger chromosomaler Strukturtypen in den alten und den neuen Populationen, aber auch die systematischen Änderungen, die sich bei diesen Populationen im Laufe der Zeit zwischen den beiden Untersuchungen ereignet haben. Eine Zunahme in den Häufigkeiten jener Chromosomenstrukturen, die typisch für südliche Breiten sind, und eine Abnahme solcher Strukturen, die häufig in nördliche Breiten sind, konnte beobachtet werden - in den mediterranen, den atlantischen und den mitteleuropäischen Populationen in der gleichen Weise. Außerdem haben die genetischen Distanzen der neuen Populationen zu einer südlichen Referenzpopulation im Vergleich zu denen der alten Populationen abgenommen. Diese Veränderungen wurden vermutlich von klimatischen Faktoren bestimmt, die mit der geographischen Breite korreliert sind. Insbesonders erscheint die Annahme, daß die globale Erwärmung für die Veränderungen des Chromosomenpolymorphismus dafür verantwortlich ist, ziemlich wahrscheinlich. Ob diese systematischen Transformationen des Chromosomenpolymorphismus ein Konsequenz lokaler Anpassungen oder auf Migration aus dem Süden zurückzuführen sind, bleibt eine offene Frage. [source] SEASONAL CYCLES OF ALLOZYME-BY-CHROMOSOMAL-INVERSION GAMETIC DISEQUILIBRIUM IN DROSOPHILA SUBOBSCURAEVOLUTION, Issue 4 2003Francisco Rodríguez-Trelles Abstract Allozyme loci are frequently found non randomly associated to the chromosomal inversions in which they are included in Drosophila. Two opposite views compete to explain strong allozyme-by-inversion gametic disequilibria: they result from natural selection or, conversely, merely represent remnants of associations accidentally established at the origin of inversions. Empirical efforts aimed at deciding between adaptive and historical scenarios have focused on the spatial distribution of disequilibria. Yet, the evolutionary significance of these associations remains uncertain. I report here the results of a time-series analysis of the seasonal variation of alleles at six allozyme loci (Acph, Lap, Pept-1, Ao, Mpi, and Xdh) in connection with the O chromosomal polymorphisms of D. subobscura. The findings were: (1) in the segment I of the O chromosome, Lap and Pept-1 allozymes changed seasonally in a cyclical fashion within the ST gene arrangement, but they changed erratically within the 3+4 gene configuration; (2) the frequencies of Lap111 and Pept-10,40 within ST dropped to their lowest values in early and late summer, respectively, when the seasonal level of the ST arrangement is lowest. Furthermore, Lap1,11 and Pept-10,40 covary with ST only within these seasons, yet in a fashion inconsistent with these alleles having a major influence on the dynamics of the inversion; (3) seasonal cycling of alleles within inversions were not detected at Acph, Ao, Mpi, and Xdh, yet these loci are nearly monomorphic at the study population, and/or their sampled series were shorter than those for Lap and Pept-1; and (4) simply monitoring allozyme frequencies separately for each inversion proved to be superior, for evidencing the seasonal cycles of the disequilibria, to the use of the D' coefficient of association. Observed seasonal cycles of allozymes within inversions likely reflect natural selection. [source] Use of specific PCR primers to identify three important industrial species of Saccharomyces genus: Saccharomyces cerevisiae, Saccharomyces bayanus and Saccharomyces pastorianusLETTERS IN APPLIED MICROBIOLOGY, Issue 2 2010G.V. De Melo Pereira Abstract Aim:, To develop species-specific primers capable of distinguishing between three important yeast species in alcoholic fermentation: Saccharomyces bayanus, Saccharomyces cerevisiae and Saccharomyces pastorianus. Methods and Results:, Two sets of primers with sequences complementary to the HO genes from Saccharomyces sensu stricto species were used. The use of the ScHO primers produced a single amplificon of c. 400 or 300 bp with species S. cerevisiae and S. pastorianus, respectively. The second pair of primers (LgHO) was also constructed, within the HO gene, composed of perfectly conserved sequences common for S. bayanus species, which generate amplicon with 700 bp. No amplification product was observed in the DNA samples from non- Saccharomyces yeasts. Saccharomyces species have also been characterized via electrophoretic karyotyping using pulsed-field gel electrophoresis to demonstrate chromosomal polymorphisms and to determine the evolutionary distances between these species. Conclusions:, We conclude that our novel species-specific primers could be used to rapidly and accurately identify of the Saccharomyces species most commonly involved in fermentation processes using a PCR-based assay. Significance and Impact of the Study:, The method may be used for routine identification of the most common Saccharomyces sensu stricto yeasts involved in industrial fermentation processes in less than 3 h. [source] | ||||||||||