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Cell Tracking (cell + tracking)
Selected AbstractsMR imaging for the longevity of mesenchymal stem cells labeled with poly- L -lysine,Resovist complexesCONTRAST MEDIA & MOLECULAR IMAGING, Issue 2 2010Gang Liu Abstract Superparamagnetic iron oxide (SPIO) nanoparticles are emerging as ideal probes for noninvasive cell tracking. In this study, poly- L -lysine (PLL) was mixed with Resovist to form the PLL,Resovist complexes and the control of the complexes formed by PLL and Resovist and their subsequent properties was easily achievable. MSCs could be safely and efficiently labeled for MR imaging using PLL,Resovist complexes (w/w 0.01:1) and the labeled MSCs could be detected to have definite decreased signal intensity on T2 -weight imaging until 20 days with standard 1.5,T MR equipment. This study describes a simple protocol to label MSCs using PLL,Resovist complexes and the results presented in our study can provide a basis for the application of PLL,Resovist complexes cell labeling. Copyright © 2010 John Wiley & Sons, Ltd. [source] Nanoparticles as tools to study and control stem cellsJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 4 2009L. Ferreira Abstract The use of nanoparticles in stem cell research is relatively recent, although very significant in the last 5 years with the publication of about 400 papers. The recent advances in the preparation of some nanomaterials, growing awareness of material science and tissue engineering researchers regarding the potential of stem cells for regenerative medicine, and advances in stem cell biology have contributed towards the boost of this research field in the last few years. Most of the research has been focused in the development of new nanoparticles for stem cell imaging; however, these nanoparticles have several potential applications such as intracellular drug carriers to control stem cell differentiation and biosensors to monitor in real time the intracellular levels of relevant biomolecules/enzymes. This review examines recent advances in the use of nanoparticles for stem cell tracking, differentiation and biosensing. We further discuss their utility and the potential concerns regarding their cytotoxicity. J. Cell. Biochem. 108: 746,752, 2009. © 2009 Wiley-Liss, Inc. [source] Analyzing cell fate control by cytokines through continuous single cell biochemistryJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 2 2009Michael A. Rieger Abstract Cytokines are important regulators of cell fates with high clinical and commercial relevance. However, despite decades of intense academic and industrial research, it proved surprisingly difficult to describe the biological functions of cytokines in a precise and comprehensive manner. The exact analysis of cytokine biology is complicated by the fact that individual cytokines control many different cell fates and activate a multitude of intracellular signaling pathways. Moreover, although activating different molecular programs, different cytokines can be redundant in their biological effects. In addition, cytokines with different biological effects can activate overlapping signaling pathways. This prospect article will outline the necessity of continuous single cell biochemistry to unravel the biological functions of molecular cytokine signaling. It focuses on potentials and limitations of recent technical developments in fluorescent time-lapse imaging and single cell tracking allowing constant long-term observation of molecules and behavior of single cells. J. Cell. Biochem. 108: 343,352, 2009. © 2009 Wiley-Liss, Inc. [source] Conserved fate and function of ferumoxides-labeled neural precursor cells in vitro and in vivoJOURNAL OF NEUROSCIENCE RESEARCH, Issue 5 2010Mikhal E. Cohen Abstract Recent progress in cell therapy research for brain diseases has raised the need for non-invasive monitoring of transplanted cells. For therapeutic application in multiple sclerosis, transplanted cells need to be tracked both spatially and temporally, in order to assess their migration and survival in the host tissue. Magnetic resonance imaging (MRI) of superparamagnetic iron oxide-(SPIO)-labeled cells has been widely used for high resolution monitoring of the biodistribution of cells after transplantation into the central nervous system (CNS). Here we labeled mouse glial-committed neural precursor cells (NPCs) with the clinically approved SPIO contrast agent ferumoxides and examined their survival and differentiation in vitro, as well as their functional response to environmental signals present within the inflamed brain of experimental autoimmune encephalomyelitis (EAE) mice in vivo. We show that ferumoxides labeling does not affect NPC survival and pluripotency in vitro. Following intracerebroventricular (ICV) transplantation in EAE mice, ferumoxides-labeled NPCs responded to inflammatory cues in a similar fashion as unlabeled cells. Ferumoxides-labeled NPCs migrated over comparable distances in white matter tracts and differentiated equally into the glial lineages. Furthermore, ferumoxides-labeled NPCs inhibited lymph node cell proliferation in vitro, similarly to non-labeled cells, suggesting a preserved immunomodulatory function. These results demonstrate that ferumoxides-based MRI cell tracking is well suited for non-invasive monitoring of NPC transplantation. © 2009 Wiley-Liss, Inc. [source] Imaging single mammalian cells with a 1.5 T clinical MRI scannerMAGNETIC RESONANCE IN MEDICINE, Issue 5 2003Paula Foster-Gareau Abstract In the present work, we demonstrate that the steady-state free precession (SSFP) imaging pulse sequence FIESTA (fast imaging employing steady state acquisition) used in conjunction with a custom-built insertable gradient coil and customized RF coils can be used to detect individual SPIO-labeled cells using a commonly available 1.5 T clinical MRI scanner. This work provides the first evidence that single-cell tracking will be possible using clinical MRI scanners, opening up new possibilities for cell tracking and monitoring of cellular therapeutics in vivo in humans. Magn Reson Med 49:968,971, 2003. © 2003 Wiley-Liss, Inc. [source] Development of a Quadruple Imaging Modality by Using NanoparticlesCHEMISTRY - A EUROPEAN JOURNAL, Issue 37 2009Won Hwang Abstract The combination of nanotechnology with molecular imaging has great potential for the development of diagnostics and therapeutics, and multimodal imaging enables versatile applications from cell tracking in animals to clinical applications. Herein, we report a multimodal nanoparticle imaging system that is capable of concurrent fluorescence, bioluminescence, bioluminescence resonance energy transfer (BRET), positron emission tomography (PET) and magnetic resonance (MR) imaging in vivo. A cobalt,ferrite nanoparticle surrounded by rhodamine (MF) was conjugated with luciferase (MFB) and p -SCNbnNOTA (2-(4-isothiocyanatobenzyl)-1,4,7-triazacyclonane-1,4,7-triacetic acid) followed by 68GaCl3 (magnetic-fluorescent-bioluminescent-radioisotopic particle, MFBR). Confocal microscopy revealed good transfection efficiency of MFB into cells and BRET was also observed in MFB. A good correlation among rhodamine, luciferase, and 68GaCl3 was found in MFBR, and the activities of each imaging modality increased dose-dependently with the amount of MFBR in the C6 cells. In vivo optical images were acquired from the thighs of mice after intramuscular and subcutaneous injections of MFBR-laden cells. MicroPET and MR images showed intense radioactivity and ferromagnetic intensities with MFBR-laden cells. The multimodal imaging strategy could be used as potential imaging tools to track cells. [source] | |||||||||||||