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Cell Strains (cell + strain)
Selected AbstractsInvestigation of Protective Reactions Against Cadmium Toxicity in the Cells Established from a Transgenic Mouse Deficient in the Metallothionein GenesJOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (ELECTRONIC), Issue 2 2003Tetsuya Abe Objective:, To characterize a fibroblast cell strain which we established from an metallothionein (MT) knock-out (KO) mouse and to determine whether expression of the Hsp genes induced by cadmium is related to expression of the MT-I and -II genes. Methods:, We established a fibroblast cell strain (named "MT-KO2") derived from the peritoneum of an MT-KO mouse which is deficient in the MT-I and -II genes. We determined an expression of MT-I, Hsp32 and Grp 78 genes by Northern blot analysis. Results:, The mRNA level of MT-I, an isoform of the MT gene products, was induced dose-dependently in responce to increasing concentrations of CdCl2 (5,25 µM) in a fibroblast cell strain derived from the peritoneum of an MT wild type mouse (named "MT-W3"). But it was not induced in MT-KO2 cells after the same treatment. There was no significant difference between MT-KO2 and MT-W3 cells in a concentration of intracellular glutathione (reduced form) under normal conditions. MT-KO2 cells were not more sensitive to cytotoxicity of CdCl2 than in MT-W3 cells. Expression of the Hsp32 gene was more extensively enhanced in MT-KO2 cells than in MT-W3 cells after treatment with 5,10 µM CdCl2 for 5 hours. Furthermore, the cellular concentration of reduced glatathione (GSH) was also more increased in MT-KO2 cells than in MT-W3 cells after treatment with 50 µM CdCl2 for 3 hours. Conclusions:, Expression of the Hsp32 gene tends to be increased in MT-KO2 cells in response to cadmium exposure. The expression of the Hsp32 gene and increase in the cellular concentration of GSH may be augmented to compensate for the impaired expression of the MT genes in MT-KO2 cells. [source] High salt-treatment-induced Na+ extrusion and low salt-treatment-induced Na+ accumulation in suspension-cultured cells of the mangrove plant, Bruguiera sexangulaPLANT CELL & ENVIRONMENT, Issue 10 2001M. Kura-Hotta Abstract A suspension-cultured cell strain of the mangrove plant (Bruguiera sexangula) was established from a callus culture and maintained in an amino acid medium in the absence of NaCl. NaCl non-adapted cells were transferred to media containing 0,200 mm NaCl. The initial growth rate decreased gradually with increasing salt concentrations. However, at up to 150 mm NaCl, cell number growth at the highest point was almost the same as that at lower salt concentrations. Cells even continued to grow in the presence of 200 mm NaCl. Cells incubated in a medium containing 50 mm NaCl for 3 weeks accumulated Na+, while those incubated in 150 mm NaCl for 2 d showed only a transient increase in Na+ and Cl, concentrations. In the latter treatment, the intracellular concentration of Na+ returned to the original low level within 2 weeks. It took a longer time for Cl, to return to its original level. As a result, the Na+ and Cl, concentrations in cells cultured with 50 mm NaCl were much larger than those in cells cultured with 150 mm NaCl. The intracellular distribution of ions after transfer to the medium containing 150 mm NaCl was analysed by isolating the vacuoles. Treatment with amiloride, an inhibitor of the Na+/H+ antiporter, suppressed the recovery of Na+ to the original level in the cells. Treatment with 150 mm NaCl for 3 d stimulated the activities of both the vanadate-dependent H+ -ATPase and the Na+/H+ antiporter in the plasma membrane fraction. [source] Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide YINTERNATIONAL ENDODONTIC JOURNAL, Issue 10 2010S. A. Killough Killough SA, Lundy FT, Irwin CR. Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y. International Endodontic Journal, 43, 835,842, 2010. Abstract Aim, To investigate whether dental pulp fibroblasts express neuropeptide Y (NPY) and NPY-Y1 in vitro and to determine the effects of the cytokines including interlukin-1, (IL-1,), TGF- ,1, substance P and NPY on the expression of NPY Y1. Methodology, Three primary fibroblast cell strains were obtained from freshly extracted human third molar teeth. RT-PCR was utilized to detect expression of NPY and mRNA expression. Membrane protein samples were isolated, and protein expression was determined by Western blotting. Radioimmunoassay was used to quantify NPY expression in healthy (n = 35) and carious (n = 39) whole pulp samples, and the student's t -test was used to test for statistical significance. In addition, the 3-(4,5-Dimethylthiazol,2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assay fibroblast cell growth. Results, mRNA transcripts were found in all three fibroblast cell populations with the cytokines having a stimulatory effect on its expression (P < 0.05). NPY mRNA was not detected in the cell strains. NPY-Y1 receptor protein expression was visualized by Western blotting, and there was no effect of IL-1, or TGF- ,1 on its expression. The mean concentration of NPY-Ir determined by radioimmunoassay in non-carious teeth was 19.40 ng g,1 (±17.03 SD) compared to 29.95 ng g,1 (±20.99 SD) in carious teeth (P < 0.05). Conclusion, Human dental pulp fibroblasts express, but do not synthesize, NPY, demonstrating that the fibroblast is a target cell for NPY. The effect of proinflammatory cytokines suggests that fibroblasts play a neuroimmunomodulatory role in the pulpal response to dental caries and injury. [source] Cytotoxic activity of an octadecenoic acid extract from Euphorbia kansui (Euphorbiaceae) on human tumour cell strainsJOURNAL OF PHARMACY AND PHARMACOLOGY: AN INTERNATI ONAL JOURNAL OF PHARMACEUTICAL SCIENCE, Issue 2 2008Farong Yu We have investigated the cytotoxic and antitumour activity of an octadecenoic acid extract, mainly containing oleic and linoleic acids, from Euphorbia kansui on human gastric (SGC-7901), hepatocellular carcinoma (BEL-7402), and leukaemia (HL-60) tumour cell strains. Significant and dose-dependent antiproliferation effects were observed on tumour cells from the dose of 3.2 ,g mL,1, which were comparable with or better than those of the common antitumour agent 5-fluorouracil. Results from the clone formation assay and flow cytometry indicated that the mixture of octadecenoic acids resulted in a dose-dependent reduction in the number of tumour cells and significantly inhibited cell proliferation, with induced apoptosis and G0/G1 phase cell cycle arrest. Also, the octadecenoic acids could not only cause cell apoptosis/necrosis but also functionally and structurally damage the tumour cell membrane and cell ultra-structures. These observations encourage further clinical evaluation of the inhibitory effects of octadecenoic acids on various forms of cancer. [source] Proteome analysis of hepatocellular carcinoma cell strains, MHCC97-H and MHCC97-L, with different metastasis potentialsPROTEINS: STRUCTURE, FUNCTION AND BIOINFORMATICS, Issue 4 2004Shi-Jian Ding Abstract To better understand the mechanism underlying hepatocellular carcinoma (HCC) metastasis and to search for potential markers for HCC prognosis, differential proteome analysis on two HCC cell strains with high and low metastatic potentials, MHCC97-H and MHCC97-L, was conducted using two-dimensional (2-D) gel electrophoresis followed by matrix-assisted laser desorption/time of flight mass spectrometry and liquid chromatography ion trap mass spectrometry. Image analysis of silver-stained 2-D gels revealed that 56 protein spots showed significant differential expression in MHCC97-H and MHCC97-L cells (Student's t -test, P < 0.05) and 4 protein spots were only detected in MHCC97-H cells. Fourteen protein spots were further identified using in-gel tryptic digestion, peptide mass fingerprinting and tandem mass spectrometry. The expressions of pyruvate kinase M2, ubiquitin carboxy-terminal hydrolase L1, laminin receptor 67 kDa, S100 calcium-binding protein A4, thioredoxin and cytokeratin 19 were elevated in MHCC97-H cells. However, manganese superoxide dismutase, calreticulin precursor, cathepsin D, lactate dehydrogenase B, non-metastatic cell protein 1, cofilin 1 and calumenin precursor were down-regulated in MHCC97-H cells. Intriguingly, most of these identified proteins have been reported to be associated with tumor metastasis. The functional implications of alterations in the levels of these proteins are discussed. [source] | ||||||||||