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Cationic Conjugated Polymer (cationic + conjugated_polymer)
Selected AbstractsDNA Detection: Intercalating Dye Harnessed Cationic Conjugated Polymer for Real-Time Naked-Eye Recognition of Double-Stranded DNA in Serum (Adv. Funct.ADVANCED FUNCTIONAL MATERIALS, Issue 9 2009Mater. On page 1371, Liu Bin and Pu Kan-Yi demonstrate multicolor detection of double-stranded DNA in biological media using an intercalating-dye-harnessed cationic conjugated polymer. As demonstrated in the cover image, the intercalating-dye-harnessed polymer emits blue fluorescence both in the absence and presence of single-stranded DNA in serum-containing solution, while its fluorescence gradually turns from blue to dark yellow with increasing double-stranded DNA concentration. [source] Intercalating Dye Harnessed Cationic Conjugated Polymer for Real-Time Naked-Eye Recognition of Double-Stranded DNA in SerumADVANCED FUNCTIONAL MATERIALS, Issue 9 2009Kan-Yi Pu Abstract Thiazole orange (TO), an intercalating dye, is integrated into cationic poly(fluorene- alt -phenylene) (PFP) to develop a macromolecular multicolor probe (PFPTO) for double-stranded DNA (dsDNA) detection. This polymer design not only takes advantage of the high affinity between TO and dsDNA to realize dsDNA recognition in biological media, but also brings into play the light-harvesting feature of conjugated polymers to amplify the signal output of TO in situ. PFPTO differentiates dsDNA from single-stranded DNA (ssDNA) more effectively upon excitation of the conjugated backbone relative to that upon direct excitation of TO as a result of efficient fluorescence resonance energy transfer from the polymer backbone to the intercalated TO. In the presence of dsDNA, energy transfer within PFPTO is more efficient as compared to that for free TO/PFP system, which leads to better dsDNA discriminability for PFPTO in contrast to that for TO/PFP. The distinguishable fluorescent color for PFPTO solutions in the presence of dsDNA allows naked-eye detection of dsDNA with the assistance of a hand-held UV lamp. The significant advantage of this macromolecular fluorescent probe is that naked-eye detection of label-free dsDNA can be performed in biological media in real-time. [source] Cationic Polyelectrolyte Amplified Bead Array for DNA Detection with Zeptomole Sensitivity and Single Nucleotide Polymorphism SelectivityADVANCED FUNCTIONAL MATERIALS, Issue 16 2010Chun Wang Abstract A highly sensitive strand specific DNA assay, which consists of a peptide nucleic acid (PNA) probe, a cationic conjugated polymer (PFVP), and self-assembled polystyrene beads in microwell arrays on silicon chip, is reported. PFVP, as an efficient signal amplifier and signal reporter, has been specially designed and synthesized to be compatible with commercial confocal microscopes for sensing on solid substrates. The assay operates on the net increase in negative charge at the PNA surface that occurs upon single-stranded DNA hybridization, which subsequently allows complex formation with the positively charged PFVP to favor energy transfer between the polymer and Cy5-labeled target. With maximized surface contact provided by bead arrays and signal amplification provided by PFVP, this assay allows detection of ,300 copies of Cy5-labeled DNA using a commercial confocal microscope. In addition, the same strategy is also extended for label-free DNA detection with a detection sensitivity of 150 attomole. Excellent discrimination against single nucleotide polymorphism (SNP) is also demonstrated for both Cy5-labeled and label-free target detection. This study indicates that cationic conjugated polymers have great potential to be incorporated into the widely used microarray technology for simplified process with improved detection sensitivity. [source] DNA Detection: Intercalating Dye Harnessed Cationic Conjugated Polymer for Real-Time Naked-Eye Recognition of Double-Stranded DNA in Serum (Adv. Funct.ADVANCED FUNCTIONAL MATERIALS, Issue 9 2009Mater. On page 1371, Liu Bin and Pu Kan-Yi demonstrate multicolor detection of double-stranded DNA in biological media using an intercalating-dye-harnessed cationic conjugated polymer. As demonstrated in the cover image, the intercalating-dye-harnessed polymer emits blue fluorescence both in the absence and presence of single-stranded DNA in serum-containing solution, while its fluorescence gradually turns from blue to dark yellow with increasing double-stranded DNA concentration. [source] A Cationic Water-Soluble Poly(p -phenylenevinylene) Derivative: Highly Sensitive Biosensor for Iron-Sulfur Protein Detection,MACROMOLECULAR RAPID COMMUNICATIONS, Issue 10 2006Fan Cheng Abstract Summary: A new water-soluble cationic ammonium-functionalized poly(p -phenylenevinylene) (PPV-NEtMe) was successfully synthesized and exhibited high sensitivity (Ksv,=,6.9,×,107M,1) on rubredoxin, a type of anionic iron-sulfur (Fe-S) proteins. Further investigation showed that the biosensitivity of the cationic conjugated polymer is strongly dependent on the nature of the buffer solution and the concentration of the conjugated polymer used in the analyses. The schematic diagram of anionic rubredoxin detected by PPV-NEtMe. [source] Cationic Polyelectrolyte Amplified Bead Array for DNA Detection with Zeptomole Sensitivity and Single Nucleotide Polymorphism SelectivityADVANCED FUNCTIONAL MATERIALS, Issue 16 2010Chun Wang Abstract A highly sensitive strand specific DNA assay, which consists of a peptide nucleic acid (PNA) probe, a cationic conjugated polymer (PFVP), and self-assembled polystyrene beads in microwell arrays on silicon chip, is reported. PFVP, as an efficient signal amplifier and signal reporter, has been specially designed and synthesized to be compatible with commercial confocal microscopes for sensing on solid substrates. The assay operates on the net increase in negative charge at the PNA surface that occurs upon single-stranded DNA hybridization, which subsequently allows complex formation with the positively charged PFVP to favor energy transfer between the polymer and Cy5-labeled target. With maximized surface contact provided by bead arrays and signal amplification provided by PFVP, this assay allows detection of ,300 copies of Cy5-labeled DNA using a commercial confocal microscope. In addition, the same strategy is also extended for label-free DNA detection with a detection sensitivity of 150 attomole. Excellent discrimination against single nucleotide polymorphism (SNP) is also demonstrated for both Cy5-labeled and label-free target detection. This study indicates that cationic conjugated polymers have great potential to be incorporated into the widely used microarray technology for simplified process with improved detection sensitivity. [source] Cationic Conjugated Polymer/DNA Complexes for Amplified Fluorescence Assays of Nucleases and Methyltransferases,ADVANCED MATERIALS, Issue 21 2007F. Feng A new method is developed for sensitive, homogeneous, and convenient assays of nucleases and methyltransferases using complexes of cationic conjugated polymers with DNA containing a fluorescein tag at the 5,-terminus (DNA-Fl). The cleavage of DNA by nucleases can be monitored by fluorescence spectra by observing conjugated-polymer or fluorescein emission changes in aqueous solutions. [source] | ||||||||||