Carrot Tissue (carrot + tissue)

Distribution by Scientific Domains


Selected Abstracts


INFLUENCE OF CELL SIZE AND CELL WALL VOLUME FRACTION ON FAILURE PROPERTIES OF POTATO AND CARROT TISSUE

JOURNAL OF TEXTURE STUDIES, Issue 1 2005
ARTUR ZDUNEK
ABSTRACT This article presents the influence of cell size and cell wall volume fraction on the failure parameters of potato tuber and carrot tissue. Confocal scanning laser microscope was used for obtaining images of the cell structure of the tissues. The mean cell face area and the cell wall volume fraction obtained from the images was compared with work to failure, failure stress, failure strain and secant modulus obtained in a compression test of potato and carrot tissue at two strain rates. Bigger cells and less amount of cell wall material weakened the tissue, which was visible as a linear decrease in the parameters: work to failure, failure stress and failure strain. There were differences between potato and carrot in the secant modulus. For carrot, the secant modulus changed with microstructural parameters, whereas for potato, the secant modulus did not depend on these values. The strain rate decreases all the failure properties for potato. For carrot, only the work to failure was affected by the strain rate. [source]


PCR-based identification of Pythium spp. causing cavity spot in carrots and sensitive detection in soil samples

PLANT PATHOLOGY, Issue 5 2008
S. S. Klemsdal
On the basis of ITS sequences PCR primers were designed for the identification of the five Pythium species found to be most important for the development of carrot cavity spot in Norway: P. intermedium, P. sulcatum, P. sylvaticum, P. violae and P. ,vipa'. The P. ,vipa' isolates had a unique ITS sequence, differed morphologically from all other Pythium isolates, and thus probably represent a new species. The PCR primers were species-specific with no cross-reaction to other Pythium species or to fungal isolates from carrot tested. The detection limits varied for the different primer pairs. The two most sensitive assays allowed detection of as little as 5 fg DNA. All five Pythium species could be detected in lesions from diseased carrots. Weak positive signals were obtained from some carrot samples without symptoms. PCR assays allowed detection of pathogens in soil. In samples of soil known to produce cavity spots on cropped carrots, strong signals were obtained. In several soil samples more than one of the five Pythium species could be detected. The utilization of this diagnostic PCR assay in analysis of field soil and carrot tissue might in the future be exploited to reduce the incidence of this serious carrot disease. [source]


Pythium species associated with cavity spot on carrots in Norway

ANNALS OF APPLIED BIOLOGY, Issue 2 2007
A. Hermansen
Abstract Carrot roots with cavity spot lesions from eight different counties in Norway were sampled and Pythium species were isolated on selective medium. Pythium spp. were characterised morphologically and by species-specific PCR. Laboratory experiments with inoculations of carrot roots were performed. A total of 130 isolates out of 230 Pythium -like isolates tested with PCR were identified as pathogenic species of Pythium. These were P. intermedium (29%), P. sulcatum (23%), P. sylvaticum (16%), P. violae (15%) and a possible new Pythium species designated P. ,vipa' (18%). There were some differences between geographical regions and ages of cavities regarding the frequency of the different species isolated. When rating sunken lesions in the laboratory inoculation experiments, P.,vipa' was the most aggressive and P. violae the least aggressive species. P. intermedium and P.,vipa' caused more discolouration of the infected carrot tissue than the other species. The importance of the different Pythium spp. as agents of cavity spot in Norway is discussed. [source]