Abnormal Profile (abnormal + profile)

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Medical Sciences100%

Selected Abstracts

Glucose production pathways by 2H and 13C NMR in patients with HIV-associated lipoatrophy,

MAGNETIC RESONANCE IN MEDICINE, Issue 4 2004
Brian C. Weis
Abstract Patients with HIV taking protease inhibitors were selected for the presence (five subjects) or absence (five subjects) of lipoatrophy. Following an overnight fast, subjects were given oral 2H2O in divided doses (5 mL/kg body water), [U- 13C3] propionate (10 mg/kg), and acetaminophen (1000 mg). Glucose (from plasma) or acetaminophen glucuronide (from urine) were converted to monoacetone glucose for 2H NMR and 13C NMR analysis. The fraction of plasma glucose derived from gluconeogenesis was not significantly different between groups. However, flux from glycerol into gluconeogenesis relative to glucose production was increased from 0.20 ± 0.13 among subjects without lipoatrophy to 0.42 ± 0.12 (P < 0.05) among subjects with lipoatrophy, and the TCA cycle contribution was reduced. Lipoatrophy was associated with an abnormal profile of glucose production as assessed by 13C and 2H NMR of plasma and urine. Magn Reson Med 51:649,654, 2004. Published 2004 Wiley-Liss, Inc. [source]

Inhibition of Obliterative Airway Disease Development in Murine Tracheal Allografts by Matrix Metalloproteinase-9 Deficiency

AMERICAN JOURNAL OF TRANSPLANTATION, Issue 4 2005
Félix G. Fernández
This study was designed to define the roles of matrix metalloproteinase (MMP)-2 and MMP-9 in obliterative airway disease (OAD) in heterotopic murine tracheal allografts, considered a suitable animal model for chronic lung allograft rejection. BALB/c tracheal allografts were transplanted into MMP-2-deficient (,/,) and MMP-9,/, mice. Also, wild-type recipients were treated with doxycycline, a nonspecific MMP inhibitor. After 10, 20 and 30 days, allografts were analyzed for OAD development, intragraft levels of MMP-2 and MMP-9 and the frequency and cytokine/chemokine production profile of alloreactive T cells. Allografts transplanted into wild-type mice developed OAD lesions within 30 days. These allografts revealed significant upregulation of both MMP-2 and MMP-9. Allografts transplanted into MMP-9,/, and doxycycline-treated recipients did not develop OAD. In contrast, allografts transplanted into MMP-2,/, mice developed OAD lesions with normal kinetics. Interestingly, MMP-9,/, recipients showed an enhanced T cell alloreactivity associated with an abnormal profile of cytokine/chemokine production. The enhanced T cell alloreactivity in MMP-9,/, mice was mediated by enhanced dendritic cell stimulatory capacity as well as enhanced T cell responsive capacity. These results suggest that MMP-9 plays an important role in the pathogenesis of OAD and may represent a target for the therapeutic intervention of chronic lung allograft rejection. [source]

Masses of phosphorylated neurofilaments are associated with abnormal Golgi apparatus of anterior horn neurons of ,, ,,-iminodipropionitrile-intoxicated rats

NEUROPATHOLOGY, Issue 2 2002
Makoto Uesugi
The Golgi apparatus (GA) of anterior horn neurons of rats chronically intoxicated with ,,,,-iminodipropionitrile (IDPN) in drinking water was examined with an organelle-specific antibody. The neuropile of the anterior horns contained the typical axonal spheroids associated with IDPN toxicity while the perikarya of approximately one-third of the neurons contained phosphorylated neurofilaments, which are not found in the perikarya of control rat neurons. By serial or double immunostaining with the SMI-31 and anti-MG 160 antibodies, there were no morphological changes of the GA in the majority of neurons including neurons with a mild to moderate degree of neurofilamentous accumulation. However, a few neurons with a massive accumulation of phosphorylated neurofilaments contained abnormal profiles of the GA which consisted of focal clustering, reduction in size and fragmentation. The results suggest that masses of phosphorylated neurofilaments are associated with struc-tural abnormalities of the GA. [source]

Field Trial on Progesterone Cycles, Metabolic Profiles, Body Condition Score and their Relation to Fertility in Estonian Holstein Dairy Cows

REPRODUCTION IN DOMESTIC ANIMALS, Issue 4 2008
J Samarütel
Contents Resumption of luteal activity postpartum and fertility were investigated in an Estonian Holstein high milk production and good fertility dairy herd. Body condition was scored after every 10 days in 54 multiparous dairy cows (71 lactations) calving inside from December to March during 4-year period. Blood samples were taken 1,14 days before calving and 1,14, 28,42 and 63,77 days after calving: analytes estimated were serum aspartate aminotransferase (AST), glucose, ketone bodies, total cholesterol, non-esterified fatty acids and triglycerides. The general linear mixed model was used to compare the data for cows with different characteristics in luteal activity postpartum based on their milk progesterone profiles. Forty-five per cent of cases had abnormal profiles; delayed resumption of ovarian cyclicity postpartum (DC) was the most prevalent abnormality. There was no difference in body condition scores between the groups. The DC and prolonged luteal phase groups had higher serum AST activity (p < 0.01) 1,14 days postpartum compared with normal group. The DC group also had higher cholesterol and triglyceride values (p < 0.05) 28,42 days postpartum and higher milk fat/protein ratio (p < 0.01) on the first month of lactation compared with normal profile group. Despite long post-calving anoestrous period (71 ± 5.0 days; mean ± SEM) DC group had 64.7% first service pregnancy rate (normal group 48.6% and PLP group 37.5%). This study did not find any detrimental effect of prolonged anovulatory period postpartum on subsequent fertility. [source]