Brilliant Blue (brilliant + blue)

Distribution by Scientific Domains

Kinds of Brilliant Blue

  • coomassie brilliant blue

  • Terms modified by Brilliant Blue

  • brilliant blue g

  • Selected Abstracts

    High-efficiency protein extraction from polyacrylamide gels for molecular mass measurement by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry

    ELECTROPHORESIS, Issue 6 2005
    Ya Jin
    Abstract A simple and fast method of protein extraction from Coomassie Brilliant Blue (CBB)-stained polyacrylamide gels suited for molecular mass measurement of proteins by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) is reported. Proteins in CBB-stained gel pieces were extracted by a 10-min soaking in 0.1,M NaOH at 25C. The recovery of this one-step extraction method was 34,73% for proteins <67,kDa. CBB adduction to proteins during mass spectrometric analysis was avoided by a destaining step before the alkaline extraction. The molecular mass values of the extracted proteins coincided with those of purified proteins within 0.01,0.10% deviation for all the proteins <36,kDa. Because of the high extraction recovery, mass measurement was possible for the proteins extracted from CBB-stained gels with loaded protein quantities as little as 34,ng for cytochrome,c, ,-lactalbumin, myoglobin, ,-lactoglobulin, trypsinogen, and carbonic anhydrase (12.4,29.0,kDa), 340,ng for glyceraldehyde-3-phosphate dehydrogenase (35.6,kDa) and albumin (66.3,kDa). This method provides a highly efficient approach to utilize CBB-stained one- or two-dimensional gels for whole protein analysis using MALDI-TOF-MS. [source]

    Comparison of fluorescent stains: Relative photostability and differential staining of proteins in two-dimensional gels

    ELECTROPHORESIS, Issue 15 2004
    Gary B. Smejkal
    Abstract The fluorescence of proteins stained with Deep Purple and SYPRO Ruby was measured over a time course of UV transillumination to determine the relative photostability of each stain. Mean spot fluorescence (n = 200 matched spots) in gels stained with Deep Purple decreased 27% following 2 min of UV transillumination, compared to SYPRO Ruby, which decreased 17%. After 19 min, an 83% decrease in Deep Purple fluorescence was observed, compared to 44% for SYPRO Ruby. By interpolation, the half-life of Deep Purple fluorescence was estimated to be approximately 6 min. The half-life of SYPRO Ruby fluorescence was not reached during the 19 min time course. Further, differential staining of proteins was observed in gels stained with Deep Purple and SYPRO Ruby as compared to colloidal Coomassie Brilliant Blue and silver staining. [source]

    A systematic investigation into the recovery of radioactively labeled proteins from sodium dodecyl sulfate-polyacrylamide gels

    ELECTROPHORESIS, Issue 1 2004
    Shaobo Zhou
    Abstract We report the results of a systematic investigation designed to optimize a method for quantifying radioactivity in proteins in sodium dodecyl sulfate-polyacrylamide gels. The method involves dissolving appropriately sized pieces of gel in hydrogen peroxide and heating to 70C overnight followed by liquid scintillation counting. H2O2 had no effect on the count rates of [14C]bovine serum albumin (BSA) when counted in a conventional liquid scintillation system, and the count rates remained stable for several days. Temperatures below 70C resulted in incomplete extraction of radioactivity from gels containing [14C]BSA, but there was also a significant reduction in count rates in samples incubated at 80C. At 70C recovery was not affected by the amount of sample loaded onto the gel or by the staining procedure (Coomassie Brilliant Blue or SYPRO Ruby). Recoveries were in the range of 89,94%, and the coefficient of variation for five replicate samples was 5,10%. This method offers a reliable way of measuring the amount of radioactivity in proteins that have been separated by electrophoresis. It may be useful, for example, in quantitative metabolic labeling experiments when it is necessary to know precisely how much tracer has been incorporated into a particular protein. [source]

    Quantifying dye tracers in soil profiles by image processing

    I. Forrer
    Summary Developing and testing models for solute transport in the field requires experimental data on the spreading of solutes in the soil. Obtaining such data is costly, and a substantial part of the total costs is in the preparation and chemical analysis of the tracing compounds in the gathered samples. We developed a cheap method to quantify the concentration of the mobile dye tracer Brilliant Blue FCF from digitized photographs of stained soil profiles, and we have tested it in the field. Soil sampling and chemical analyses were necessary only to establish a calibration relation between the dye content and the colour of the soil. The digital images were corrected for geometrical distortions, varying background brightness, and colour tinges, and then they were analysed to determine the soil colour at sampling points in the profiles. The resident concentration of the dye was modelled by polynomial regression with the primary colours red, green, blue and the soil depth as explanatory variables. Concentration maps of Brilliant Blue were then computed from the digitized images with a spatial resolution of 1 mm. Validation of the technique with independent data showed that the method predicted the concentration of the dye well, provided the corrected images contained only the colours included in the calibration. [source]

    Preparation and characterization of complex gel of type I collagen and aluminosilicate containing imogolite nanofibers

    Asuka Nakano
    Abstract Complex gel materials of Type I collagen and aluminosilicate containing imogolite nanofibers were prepared as opaque gel by mixing an acidic fine dispersion of aluminosilicate with an acidic solution of collagen. The product was stained blue by Coomassie Brilliant Blue (CBB), indicating that the gel contained collagen. A white sponge was obtained after lyophilization of the complex gel. Elemental analysis revealed that the complex contains C, H, N, Al, and Si atoms; and the compositional ratio of aluminosilicate/collagen (w/w) was calculated as 0.75 for the complex gel when aluminosilicate was mixed with an equal quantity of collagen. Transmission electron microscope (TEM) observation showed that aluminosilicate nanofibers were homogeneously distributed in the collagen matrix. The thermogravimetric analysis (TGA) curve of the complex was not a simple summation of each components, and especially, the weight loss step corresponding to detachment of the adsorbed water observed in aluminosilicate became difficult to distinguish, suggesting that the adsorbed water was removed in the complexation. 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010 [source]

    Differential display proteomic analysis of Picea meyeri pollen germination and pollen-tube growth after inhibition of actin polymerization by latrunculin B

    THE PLANT JOURNAL, Issue 2 2006
    Yanmei Chen
    Summary To investigate roles of the actin cytoskeleton in growth of the pollen tube of Picea meyeri, we used the actin polymerization inhibitor latrunculin B (LATB) under quantitatively controlled conditions. At low concentrations, LATB inhibited polymerization of the actin cytoskeleton in the growing pollen tube, which rapidly inhibited tip growth. The proteomic approach was used to analyse protein expression-profile changes during pollen germination and subsequent pollen-tube development with disturbed organization of the actin cytoskeleton. Two-dimensional electrophoresis and staining with Coomassie Brilliant Blue revealed nearly 600 protein spots. A total of 84 of these were differentially displayed at different hours with varying doses of LATB, and 53 upregulated or downregulated proteins were identified by mass spectrometry. These proteins were grouped into distinct functional categories including signalling, actin cytoskeleton organization, cell expansion and carbohydrate metabolism. Moreover, actin disruption affected the morphology of Golgi stacks, mitochondria and amyloplasts, along with a differential expression of proteins involved in their functions. These findings provide new insights into the multifaceted mechanism of actin cytoskeleton functions and its interaction with signalling, cell-expansion machinery and energy-providing pathways. [source]

    Iridescent hindwing patches in the Pipevine Swallowtail: differences in dorsal and ventral surfaces relate to signal function and context

    FUNCTIONAL ECOLOGY, Issue 4 2010
    Ronald L. Rutowski
    Summary 1.,Iridescent colour signals are directional but, like diffusely reflected colours, vary within and among species in ways that may be adaptations to specific types of receivers in specific light environments. 2.,The hindwings of pipevine swallowtail butterflies exhibit brilliant blue and iridescent colour patches on the ventral surface in both sexes and on the dorsal wing surface in males. Evidence suggests that the ventral iridescent blue is a component of the warning coloration of this distasteful species, while the dorsal blue iridescent wing area is a sexual signal. Given differences in the function and ecological context of signal production, we analysed reflectance spectra from the iridescent blue areas of both field-caught and laboratory-reared animals to test several predictions about the iridescent colour patches on these wing surfaces. 3.,The ventral blue patches in the warning coloration of males and females should be most visible early and late in the day, due to wing orientation relative to sun angle. We therefore predicted that these iridescent colour patches would be brighter and of longer wavelengths than the male dorsal blue patch displayed during midday courtships. The prediction about reflectance intensity was supported but the prediction about hue was not. 4.,We predicted that the sexually selected dorsal hindwing iridescence of males would be more variable among individuals and condition dependent than the naturally selected ventral iridescent colour patches. To assess variation and condition dependence, laboratory-reared and field-captured individuals were compared. The prediction about variation was not supported, but only the dorsal wing surfaces showed evidence of being condition dependent. 5.,We investigated whether development of dorsal and ventral blue iridescence was coupled by determining correlations in colour properties between the wing surfaces. Our finding of positive correlations indicated a potential developmental constraint in the evolution of colour differences between the two wing surfaces. 6.,Results of this study suggest that some properties of iridescent coloration on the hindwing of the pipevine swallowtail (especially intensity) may have been fine-tuned by evolution in response to prevailing ambient light conditions and viewing perspectives that differ among types of signal receivers. [source]

    Identification of four low molecular and water-soluble proteins from grape (Vitis vinifera L.) seeds

    Ting Zhou
    Summary Profiles of soluble proteins isolated from mature seeds of grape (Vitis vinifera L.) pomace were studied using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) coupled with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI,TOF,MS). Two-dimensional gels stained with Coomassie brilliant blue revealed more than fifty protein spots. Four abundant protein spots showing low molecular weight (Mr) and wide isoelectric point (pI) were analysed by MALDI,TOF,MS, resulting in their identification. Taken together, these results suggest that identified proteins may be linked to seed development and metabolism, but more instructive is that they have some potential functions for future food application. These results provide some insights into conversion of grape processing wastes into useful products or even as raw material for other industries. [source]

    Isolation and characterization of a novel copper-inducible metallothionein gene of a ciliate, Tetrahymena tropicalis lahorensis

    Raheela Chaudhry
    Abstract The two isoforms of copper metallothionein (CuMT) gene of a copper resistant ciliate, Tetrahymena tropicalis lahorensis (Ttl), have been isolated and characterized. The molecular cloning and nucleotide sequencing of cDNAs coding for the two CuMT isoforms revealed that TtlCuMT1 gene has 300, while TtlCuMT2 has 327 nucleotides, both with ATG as the initiation codon and TGA as the translational termination codon. TAG codes for glutamine in TtlCuMT2 gene which is peculiar to Tetrahymena. The deduced or translated TtlCuMT1 and TtlCuMT2 peptide sequences contain 100 and 108 amino acid residues including 28 and 32 cysteine residues, respectively. The amino acid sequences of TtlCuMT1 and TtlCuMT2 have special features of two and three CXCXXCXCXXCXC intragenic tandem repeats with a conserved structural pattern of cysteine, respectively. The predicted tertiary structures of these two isoforms indicate two domains. Domain I and the initial part of domain II showed >98% homology with other Tetrahymena CuMT. On the basis of the differences in the domain II, the metallothionein subfamily 7b can be divided into two groups, one (TtlCuMT1) comprising >100 amino acids and the other (TtlCuMT2) comprising <100 amino acids. This is a novel finding of the present study as no such report on this type of classification exists at the moment. TtlCuMT1 has 95%, while TtlCuMT2 has 97% resemblance with the previously reported CuMT genes of Tetrahymena spp. SDS-PAGE analysis using fluorescent probe as well as coomassie brilliant blue staining also confirmed the presence of metallothionein. J. Cell. Biochem. 110: 630,644, 2010. 2010 Wiley-Liss, Inc. [source]