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Biological Response (biological + response)
Terms modified by Biological Response Selected AbstractsQSARs for aromatic hydrocarbons at several trophic levelsENVIRONMENTAL TOXICOLOGY, Issue 2 2006Walter Di Marzio Abstract Quantitative structure,activity relationships (QSARs) with aromatic hydrocarbons were obtained. Biological response was measured by acute toxicity of several aquatic trophic levels. The chemicals assayed were benzene, toluene, ethylbenzene, o -xylene, m -xylene, p -xylene, isopropylbenzene, n -propylbenzene, and butylbenzene. Acute toxicity tests were carried out with Scenedesmus quadricauda, as representative of primary producers; Daphnia spinulata, a zooplanctonic cladoceran; Hyalella curvispina, a benthic macroinvertebrate; and Bryconamericus iheringii, an omnivorous native fish. The EC50 or LC50 was calculated from analytical determinations of aromatic hydrocarbons. Nonlinear regression analysis between the logarithm of the octanol,water partition coefficient (log Kow) of each compounds and the toxicity end points was performed. QSARs were positively related to increases in log Kow at all trophic levels. Intertaxonomic differences were found in comparisons of algae with animals and of invertebrates with vertebrates. We observed that these differences were not significant with a log Kow higher than 3 for all organisms. Aromatic hydrocarbons with log Kow values of less than 3 showed different toxicity responses, with algae more resistant than fish and invertebrates. We concluded that this was a result of the narcotic mode of action related to liposolubility and the ability of the compound to reach its target site in the cell. The bioconcentration factor (BCF) achieved to start nonpolar narcosis fell almost 1 order of magnitude below the BCF expected from the log Kow. Predicted critical body residues for nonpolar narcosis ranged between 2 and 1 mM. © 2006 Wiley Periodicals, Inc. Environ Toxicol 21: 118,124, 2006. [source] Genetic response to rapid climate change: it's seasonal timing that mattersMOLECULAR ECOLOGY, Issue 1 2008W. E. BRADSHAW Abstract The primary nonbiological result of recent rapid climate change is warming winter temperatures, particularly at northern latitudes, leading to longer growing seasons and new seasonal exigencies and opportunities. Biological responses reflect selection due to the earlier arrival of spring, the later arrival of fall, or the increasing length of the growing season. Animals from rotifers to rodents use the high reliability of day length to time the seasonal transitions in their life histories that are crucial to fitness in temperate and polar environments: when to begin developing in the spring, when to reproduce, when to enter dormancy or when to migrate, thereby exploiting favourable temperatures and avoiding unfavourable temperatures. In documented cases of evolutionary (genetic) response to recent, rapid climate change, the role of day length (photoperiodism) ranges from causal to inhibitory; in no case has there been demonstrated a genetic shift in thermal optima or thermal tolerance. More effort should be made to explore the role of photoperiodism in genetic responses to climate change and to rule out the role of photoperiod in the timing of seasonal life histories before thermal adaptation is assumed to be the major evolutionary response to climate change. [source] Population growth and mass mortality of an estuarine fish, Acanthopagrus butcheri, unlawfully introduced into an inland lakeAQUATIC CONSERVATION: MARINE AND FRESHWATER ECOSYSTEMS, Issue 1 2009Kimberley Smith Abstract 1.In 2006, two periods of hypoxia resulted in the death of approximately 35 tonnes of black bream (Acanthopagrus butcheri) in Lake Indoon, a small inland lake in Western Australia. 2.Acanthopagrus butcheri was the first fish species to be recorded in this lake, along with the mosquitofish (Gambusia holbrooki) which was also observed during sampling in 2006. Acanthopagrus butcheri appears to have been introduced to Lake Indoon between 1998 and 2003 and formed a self-sustaining population. It is believed to have been deliberately introduced for the purpose of creating a recreational fishery, despite the existence of substantial penalties for illegal translocation of fish in Western Australia. 3.Recent human-induced environmental changes, including rising groundwater and salinization, have probably aided the establishment of both species in Lake Indoon. The importance of salinity to recruitment success by A. butcheri was indicated by the presence of only two age classes in 2006, with estimated recruitment dates coinciding with the years of highest recorded salinity in the lake. 4.The ,fish kills' provided an opportunity to examine aspects of A. butcheri biology in a relatively low salinity environment which is atypical for this estuarine species. In particular, the recruitment period in Lake Indoon was delayed until autumn/winter, rather than spring/summer as seen in other populations. Biological responses in Lake Indoon have implications for natural populations living in estuaries with modified salinity regimes. 5.The ecological, social and economic impacts potentially arising from the introduction of fish to Lake Indoon, which is an important migratory bird habitat and a recreational amenity for local residents and tourists, illustrate the complexities of fish translocation and the need for rigorous assessment before stocking to identify potential costs and benefits. Copyright © 2008 John Wiley & Sons, Ltd. [source] The assessment of insulin resistance in manDIABETIC MEDICINE, Issue 7 2002T. M. Wallace Abstract Background Insulin resistance exists when a normal concentration of insulin produces a less than normal biological response. The ability to measure insulin resistance is important in order to understand the aetiopathology of Type 2 diabetes, to examine the epidemiology and to assess the effects of intervention. Methods We assess and compare methods of measurement and have undertaken a literature review from 1966 to 2001. Results Quantitative estimates of insulin resistance can be obtained using model assessments, clamps or insulin infusion sensitivity tests. There is considerable variation in the complexity and labour intensity of the various methods. The most well-established methods are the euglycaemic clamp, minimal model assessment and homeostatic model assessment (HOMA). No single test is appropriate under all circumstances. Conclusions There are a number of well-established tests used to measure insulin resistance: the choice of method depends on the size and type of study to be undertaken. Although the so-called ,gold-standard' test, the euglycaemic clamp, is useful for intensive physiological studies on small numbers of subjects, a simpler tool such as HOMA is more appropriate for large epidemiological studies. It is important to be aware that most techniques measure stimulated insulin resistance whereas HOMA gives an estimate of basal insulin resistance. Caution should be exercised when making comparisons between studies due to variations in infusion protocols, sampling procedures and hormone assays used in different studies. [source] Selection for discontinuous life-history traits along a continuous thermal gradient in the butterfly Aricia agestisECOLOGICAL ENTOMOLOGY, Issue 6 2005Steve Burke Abstract., 1.,Voltinism may be conceptualised as the product of development rate and the timing of diapause , two components that together translate gradual environmental variation, through periods of growth and development, into ,generational units'. This may result in very different selection pressures on diapause induction and development time in populations with different numbers of generations per year. 2.,Developmental data from univoltine and bivoltine populations of the butterfly Aricia agestis (Lepidoptera: Lycaenidae) in North Wales were used to examine larval development time and the timing of diapause, and their contribution towards voltinism in populations that occur at the same latitude and in geographic proximity to one another along a thermal gradient. 3.,The critical photoperiod for diapause induction in univoltines and bivoltines from the same latitude differed by more than 1.5 h. 4.,Development time also differed significantly between these populations, in line with predictions that bivoltines would need to exhibit shorter development times in order to achieve two complete generations per year. Shorter development times for bivoltines result in lower pupal weights, suggesting a trade-off exists between generation number and body size that may dictate the position of the transition zone between the two life-history strategies. Analysis of development times in a third population, from southern England, with greater thermal availability than those from North Wales, further supports this hypothesised trade-off. 5.,To achieve the conversion of a continuous thermal gradient into the binary biological response from univoltism to bivoltism, bivoltines speed up development, reduce adult body size and shift their diapause induction response. [source] Urinary concentrations of bisphenol A in relation to biomarkers of sensitivity and effect and endocrine-related health effectsENVIRONMENTAL AND MOLECULAR MUTAGENESIS, Issue 8 2006Mihi Yang Abstract The impact of endocrine-disrupting chemicals (EDCs) on human health is not yet clear because of difficulties in ascertaining their biological effects. In the present study, we evaluated exposure to the EDC, bisphenol A (BPA), in 172 Koreans in relation to biomarkers of susceptibility and effect. The subjects completed questionnaires, which documented occupation, education, lifestyle factors, potential sources of BPA-exposure, and the occurrence of self-diagnosed endocrine disorders. None of the subjects were occupationallay exposed to BPA; however, urinary levels of conjugated BPA, determined by HPLC/FD, ranged from 0.03,62.4 ,g/l (median, 7.86). The frequencies of potential susceptibility biomarkers, the UGT1A6-Arg184Ser and the SULT1A1- Arg213His polymorphisms, were not associated with urinary BPA levels, either as single genes or in combination. Indirect effects of BPA exposure on the susceptibility to mutagens were evaluated by comparing urinary BPA concentrations with MNNG-induced sister-chromatid exchange (SCE) in lymphocytes cultured from the subjects. BPA exposure showed marginal or significant associations with theSCEs induced by the low doses of MNNG (0,0.4 mM). However, there was no overall association between urinary BPA levels and MNNG-induced frequency at doses ranging from 0.2,0.6 mM. Finally, we did not detect an association between urinary BPA concentration and endocrine-related disorders. Even though we were unable to find a strong association between BPA exposure and a biological response, possibly because of the limited number of subjects, we observed that most of the subjects were exposed to BPA. Therefore, continuous biological monitoring of BPA is a prudent measure to prevent possible BPA-related health risks. Environ. Mol. Mutagen., 2006. © 2006 Wiley-Liss, Inc. [source] Influence of Treatment Conditions on the Chemical Oxidative Activity of H2SO4/H2O2 Mixtures for Modulating the Topography of Titanium,ADVANCED ENGINEERING MATERIALS, Issue 12 2009Fabio Variola Abstract Host-tissue integration of medical implants is governed by their surface properties. The capacity to rationally design the surface physico-chemical cues of implantable materials is thus a fundamental prerequisite to confer enhanced biocompatibility. Our previous work demonstrated that different cellular processes are elicited by the nanotexture generated on titanium (cpTi) and Ti6Al4V alloy by chemical oxidation with a H2SO4/H2O2 mixture. Here, we illustrate that by varying the etching parameters such as temperature, concentration, and treatment time, we can create a variety of surface features on titanium which are expected to impact its biological response. The modified submicron and nanotextured surfaces were characterized by scanning electron (SEM) and atomic force (AFM) microscopies. Contact angle measurements revealed the higher hydrophilicity of the modified surfaces compared to untreated samples and Fourier transform infrared spectroscopy (FT-IR) established that the etching generated a TiO2 layer with a thickness in the 40,60,nm range. [source] Adaptation, extinction and global changeEVOLUTIONARY APPLICATIONS (ELECTRONIC), Issue 1 2008Graham Bell Abstract We discuss three interlinked issues: the natural pace of environmental change and adaptation, the likelihood that a population will adapt to a potentially lethal change, and adaptation to elevated CO2, the prime mover of global change. 1.,Environmental variability is governed by power laws showing that ln difference in conditions increases with ln elapsed time at a rate of 0.3,0.4. This leads to strong but fluctuating selection in many natural populations. 2.,The effect of repeated adverse change on mean fitness depends on its frequency rather than its severity. If the depression of mean fitness leads to population decline, however, severe stress may cause extinction. Evolutionary rescue from extinction requires abundant genetic variation or a high mutation supply rate, and thus a large population size. Although natural populations can sustain quite intense selection, they often fail to adapt to anthropogenic stresses such as pollution and acidification and instead become extinct. 3.,Experimental selection lines of algae show no specific adaptation to elevated CO2, but instead lose their carbon-concentrating mechanism through mutational degradation. This is likely to reduce the effectiveness of the oceanic carbon pump. Elevated CO2 is also likely to lead to changes in phytoplankton community composition, although it is not yet clear what these will be. We emphasize the importance of experimental evolution in understanding and predicting the biological response to global change. This will be one of the main tasks of evolutionary biologists in the coming decade. [source] Interleukin-6-induced proliferation of pre-B cells mediated by receptor complexes lacking the SHP2/SOCS3 recruitment sites revisitedFEBS JOURNAL, Issue 24 2001Kerstin Friederichs Interleukin-6 (IL-6) induces B-cell proliferation by binding to receptor complexes composed of a specific ,-receptor (gp80; CD126) and the signal transducing receptor subunit gp130 (CD130). Immediately after receptor complex activation, signal transducers and activators of transcription (STATs) 1 and 3 and the Src-homology domain-containing protein tyrosine phosphatase 2 (SHP2) are recruited to gp130 and subsequently tyrosine phosphorylated. The activated dimerized STATs translocate to the nucleus and bind to enhancer elements of IL-6-inducible genes. SHP2 acts as an adapter and links the Jak/STAT pathway to the Ras/Raf/MAPK cascade but it is also involved in signal attenuation. Whereas STAT3 activation appears to be crucial for all biological activities of IL-6, the requirement of SHP2-activation depends on the individual biological response analyzed. The requirement of SHP2 activation for the pre-B cell (Ba/F3) proliferation has been reported previously [Fukada, T., Hibi, M., Yamanaka, Y., Takahashi-Tezuka, M., Fujitani, Y., Yamaguchi, T., Nakajima, K. & Hirano, T. (1996) Immunity5, 449,460]. In contrast, we have recently demonstrated that the presence of a single STAT-recruitment site within gp130 is sufficient for IL-6- induced proliferation of Ba/F3 cells [Schmitz, J., Dahmen, H., Grimm, C., Gendo, C., Müller-Newen, G., Heinrich, P.C. & Schaper, F. (2000) J. Immunol.164, 848,854]. To unravel this discrepancy we analyzed the IL-6-induced dose-dependent proliferation of Ba/F3 cells mediated by receptor complexes lacking SHP2/SOCS3 recruitment sites. Surprisingly, pre-B cells, after stimulation with low amounts of IL-6, proliferate much more efficiently in the absence of the activated SHP2 than in the presence of the tyrosine phosphatase. Therefore, SHP2 activation appears to be relevant for IL-6-induced proliferation only after stimulation with very large amounts of IL-6. [source] Interaction of Zoospores of the Green Alga Ulva with Bioinspired Micro- and Nanostructured Surfaces Prepared by Polyelectrolyte Layer-by-Layer Self-AssemblyADVANCED FUNCTIONAL MATERIALS, Issue 12 2010Xinyu Cao Abstract The interaction of spores of Ulva with bioinspired structured surfaces in the nanometer,micrometer size range is investigated using a series of coatings with systematically varying morphology and chemistry, which allows separation of the contributions of morphology and surface chemistry to settlement (attachment) and adhesion strength. Structured surfaces are prepared by layer-by-layer spray-coating deposition of polyelectrolytes. By changing the pH during application of oppositely charged poly(acrylic acid) and polyethylenimine polyelectrolytes, the surface structures are systematically varied, which allows the influence of morphology on the biological response to be determined. In order to discriminate morphological from chemical effects, surfaces are chemically modified with poly(ethylene glycol) and tridecafluoroctyltriethoxysilane. This chemical modification changes the water contact angles while the influence of the morphology is retained. The lowest level of settlement is observed for structures of the order 2,µm. All surfaces are characterized with respect to their wettability, chemical composition, and morphological properties by contact angle measurement, X-ray photoelectron spectroscopy, scanning electron microscopy, and atomic force microscopy. [source] Determination of ABCB1 polymorphisms and haplotypes frequencies in a French populationFUNDAMENTAL & CLINICAL PHARMACOLOGY, Issue 4 2007Elise Jeannesson Abstract The ATP-binding cassette (ABC) transporter ABCB1, or P-glycoprotein, is a transmembrane efflux pump well known for its implication in drug transport and chemoresistance. ABCB1 substrates include either drugs, such as antiretrovirals and immunomodulators, or physiological molecules like phospholipids. Pharmacogenetic analysis of ABCB1 polymorphisms, in addition to other xenobiotic metabolizing enzymes, might help to personalize and optimize drug therapy. Indeed, some polymorphisms of ABCB1 have been implicated in susceptibility to diseases, changes in drug pharmacokinetics, and in variation of the biological response to drug treatment. In addition, variant and haplotype distributions differ depending on ethnicity. Thus, some ethnies may be at higher risk for adverse events, inefficacy of treatment or prevalence of pathologies. This study aimed to determine frequencies of ABCB1 polymorphisms and haplotypes in a sample of French healthy individuals. DNA was isolated from blood-EDTA. Polymerase chain reaction-restriction fragment length polymorphism and TaqMan single nucleotide polymorphism genotyping assays were used to genotype 227 individuals for T-129C, G-1A, A61G, G1199A, C1236T, T-76A, G2677T/A and C3435T polymorphisms. The observed frequencies of the variant allele for these eight polymorphisms are 0.04, 0.08, 0.09, 0.06, 0.42, 0.46, 0.45 and 0.46 respectively. These polymorphisms are in linkage disequilibrium and haplotype frequencies were determined, the most frequent haplotype being the one with variants at position 1236, 2677 and 3435 and wild-type alleles at the other positions. Finally, the frequencies of these eight ABCB1 polymorphisms in our French individuals supposed to be healthy population are quite similar to those described in other Caucasian populations except for the C3435T polymorphism. [source] Variation in Serripes groenlandicus (Bivalvia) growth in a Norwegian high-Arctic fjord: evidence for local- and large-scale climatic forcingGLOBAL CHANGE BIOLOGY, Issue 9 2006WILLIAM G. AMBROSE Jr. Abstract We examined the growth rate of the circumpolar Greenland Cockle (Serripes groenlandicus) over a period of 20 years (1983,2002) from Rijpfjord, a high-Arctic fjord in northeast Svalbard (80°10,N, 22°15,E). This period encompassed different phases of large-scale climatic oscillations with accompanying variations in local physical variables (temperature, atmospheric pressure, precipitation, sea ice cover), allowing us to analyze the linkage between growth rate, climatic oscillations, and their local physical and biological manifestations. Standard growth index (SGI), an ontogenetically adjusted measure of annual growth, ranged from a low of 0.27 in 2002 up to 2.46 in 1996. Interannual variation in growth corresponded to the Arctic climate regime index (ACRI), with high growth rates during the positive ACRI phase characterized by cyclonic ocean circulation and a warmer and wetter climate. Growth rates were influenced by local manifestations of the ACRI: positively correlated with precipitation and to a lesser extent negatively correlated with atmospheric pressure. A multiple regression model explains 65% of the variability in growth rate by the ACRI and precipitation at the nearest meteorological station. There were, however, complexities in the relationship between growth and physical variables, including an apparent 1 year lag between physical forcing changes and biological response. Also, when the last 4 years of poor growth are excluded, there is a very strong negative correlation with ice cover on a pan-arctic scale. Our results suggest that bivalves, as sentinels of climate change on multi-decadal scales, are sensitive to environmental variations associated with large-scale changes in climate, but that the effects will be determined by changes in environmental parameters regulating marine production and food availability on a local scale. [source] Mild haemophilia: a disease with many faces and many unexpected pitfallsHAEMOPHILIA, Issue 2010K. PEERLINCK Summary., Despite major advances in diagnosis and treatment, the management of patients with mild haemophilia (MH) remains a major challenge. Mild haemophilia is defined by factor levels between 0.05 and 0.40 IU mL,1. The bleeding associated with mild haemophilia is most frequently episodic, occurring during surgery or following trauma. Spontaneous bleeding is rare. Diagnosis is sometimes delayed because of insensitivity of screening clotting assays or discrepancies in factor VIII activity as measured by different assays. The treatment of choice in mild haemophilia A is desmopressin, which typically induces a 2,6-fold increase of factor VIII over baseline. However, desmopressin has its limitations in this setting such as the occurrence of tachyphylaxis and failure to respond in an undetermined proportion of patients. Factors underlying poor biological response or magnitude of response to desmopressin are incompletely understood. Inhibitor development in mild haemophilia is particularly distressing. This complication arises at an older age in this patient group because of infrequent need for factor VIII replacement. Inhibitors in mild haemophilia patients often cross-react with endogenous factor VIII resulting in severe spontaneous bleeding frequently in a postoperative setting. Intensive perioperative use of factor VIII and some specific mutations induce a particularly high risk for inhibitor development, but risk factors are incompletely understood. For reasons of the older age of the patients, treatment of bleeding with bypassing agents may cause major thrombotic complications. Data on therapeutic options for inhibitor eradication in patients with mild haemophilia are particularly scarce. With increased life-expectancy for all haemophilia patients, the group of elderly patients with mild haemophilia requiring major surgery will further increase. Prevention of inhibitors, particularly in this patient group, should be a major topic of interest in both clinic and research. [source] Temperature and Thermal Balance Monitoring and Control in DialysisHEMODIALYSIS INTERNATIONAL, Issue 2 2003Franti, ek Lopot Temperature and thermal balance have been studied in an effort to explain better tolerance of ultrafiltration during isolated ultrafiltration and other convective techniques as compared to conventional hemodialysis. The large number of published studies has led to the conclusion that negative thermal balance of the extracorporeal circuit ameliorates hemodynamic stability by increased vasoreactivity and increased peripheral resistance. On the other hand, measurement of dialysis efficiency (urea removal) did not unequivocally confirm the theoretically predicted decrease in efficiency of "cool" dialysis. Another suggested application of temperature and thermal balance for assessing bioincompatibility is currently hampered by the ability of existing technology to evaluate thermal parameters of the extracorporeal circuit only. Publications on impact of negative thermal balance of the extracorporeal circuit on ultrafiltration-induced changes in blood volume give contradictory results. Further studies are needed for elucidation of the impact of thermal balance on overall biological response to dialysis. [source] The genetics of aspirin resistanceINTERNATIONAL JOURNAL OF CLINICAL PRACTICE, Issue 5 2007Timothy Goodman Summary Aspirin is widely used for the prophylaxis of cardiovascular events in patients with cardiovascular risk factors or established atherosclerotic disease. However, despite aspirin treatment, a substantial number of patients experience recurrent events. Such ,aspirin resistance' is generally defined as failure of aspirin to produce an expected biological response, for example inhibition of platelet aggregation or of thromboxane A2 synthesis. Whilst its aetiology is multifactorial, genetic factors are also likely to play their part. Here we review the evidence for and against such a genetic contribution, as well as the data suggesting the involvement of specific genes. [source] Relationship between UVA protection and skin response to UV light: proposal for labelling UVA protectionINTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Issue 4 2004M. Jean-Louis Refrégier Synopsis Definition and validation of a most relevant method to assess ultravoilet A (UVA) protection is a major concern for industry, authorities and consumers. However, due to the lack of knowledge about all the biological phenomena involved, the level of UVA protection needed, the ways to assess and label it, remain controversial. In order to overcome this situation, the paper deals with the outcomes of a mathematical model to calculate the distribution between ultravoilet B (UVB) and UVA components of skin responses to UV light. Mathematical calculations of UVB and UVA erythemal components of skin response to sunlight are developed from the well-known determination procedure to calculate the sunburn protection factor (SPF) of sunscreens. The model establishes the relationship between the UVA component of skin erythemal response to overall UV radiation received from sunlight and the ratio SPF/PFAe (erythemal protection factor) where SPF is the product and PFAe is related to the UVA part of the sunlight. Depending on the efficacy profile of sunscreens, the skin erythemal response may be mainly promoted by UVB rays as it normally occurs in unprotected skin or on contrary by UVA rays. Therefore, the efficacy profile of sunscreens defines the deepness where biological events induced by sunlight take place. This new relationship pinpoints the tremendous importance of the protection afforded by sunscreen products in the UVA range when erythema is taken as biological response. By extrapolation of the model to any other biological skin response it becomes possible to predict how to improve the efficiency of sunscreen products in the future. UVA protection afforded by sunscreens should be improved until reaching the same level as the SPF protection factor so that all UV-induced biological responses could be prevented or lowered at the same extend. To enforce this improvement, a proposal to classify sunscreen products in relation with their UVA protection is made. Résumé Bien que les méfaits du rayonnement UVA soient à présent reconnus et l'importance de s'en protéger au même titre que ceux du rayonnement UVB totalement admise, l'obtention d'un consensus au niveau international, sur les méthodes pour mesurer l'efficacité des produits solaires vis-à-vis des UVA et sur les niveaux d'efficacité souhaitables, semble impossible à atteindre. Afin de tenter de surmonter les obstacles actuels, nous présentons un modèle mathématique qui permet d'établir la relation qui dèfinit le poids relatif des rayonnements UVB et UVA dans l'initiation des phénomènes biologiques engendrés par le rayonnement solaire, en fonction des caractéristiques du produit solaire utilisé et en particulier de son efficacité protectrice vis-à-vis des UVA. Dans le cas de l'érythème nous établissons ainsi que la proportion des effets engendrés par le rayonnement UVA est définie par le rapport SPF/PFAe: le SPF étant le facteur de protection contre l'érythème vis-à-vis de l,ensemble du rayonnement UV, c'est l'indice de protection affiché sur les produits; et, PFAe étant le facteur de protection du produit vis-à-vis du seul rayonnement UVA. L'extrapolation possible de ce modèle à l'ensemble des phénomènes biologiques met en évidence que le facteur de proportionnalité entre la protection globale et celle apportée vis-à-vis des UVA (SPF/PFAe) permet d'établir une classification de la qualité des systèmes filtrants en fonction de leur aptitude à prèvenir l'ensemble des méfaits du rayonnement solaire. Ce modèle démontre l'importance d'évaluer l,efficacité protectrice des produits solaires vis-à-vis du rayonnement UVA et son enseignement plus pertinent que celle de seulement évaluer l'allure des spectres d'absorption. Nous jugeons que l'application directe de ce modèle, au même titre que les méthodes d'évaluation de l,allure des spectres d'absorption, n'est aujourd'hui pas souhaitable en raison des connaissances et donc de la validation insuffisantes des méthodes in vitro en particulier pour évaluer les produits non parfaitement photostables. En conséquence, nous proposons de mettre en place une qualification qui repose sur l'évaluation de la protection UVA par les mèthodes in vivo dûment étudiées et validées telles que les méthodes PPD ou PFA. La mise en place du système proposé de qualification des produits solaires, permettrait d'apporter rapidement aux consommateurs une meilleure information sur la qualité des produits et permettrait de créer une dynamique d'amélioration de la qualité de l'ensemble des produits commercialisés. [source] Hollow, pH-sensitive calcium,alginate/poly(acrylic acid) hydrogel beads as drug carriers for vancomycin releaseJOURNAL OF APPLIED POLYMER SCIENCE, Issue 4 2010Hong-Ru Lin Abstract In this study, hollow calcium,alginate/poly(acrylic acid) (PAA) hydrogel beads were prepared by UV polymerization for use as drug carriers. The hollow structure of the beads was fortified by the incorporation of PAA. The beads exhibited different swelling ratios when immersed in media at different pH values; this demonstrated that the prepared hydrogel beads were pH sensitive. A small amount (<9%) of vancomycin that had been incorporated into the beads was released in simulated gastric fluid, whereas a large amount (,67%) was released in a sustained manner in simulated intestinal fluid. The observed drug-release profiles demonstrated that the prepared hydrogel beads are ideal candidate carriers for vancomycin delivery into the gastrointestinal tract. Furthermore, the biological response of cells to these hydrogel beads indicated that they exhibited good biological safety and may have additional applications in tissue engineering. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010 [source] Preparation, characterization, and cellular interactions of collagen-immobilized PDMS surfacesJOURNAL OF APPLIED POLYMER SCIENCE, Issue 1 2008I. Keranov Abstract Multistep procedure to biofunctionalization of (poly)dimethylsiloxane (PDMS) surfaces is present here, including plasma-based Ar+ beam treatment; acrylic acid grafting; and flexible PEG spacer coupling prior to the collagen immobilization by peptide synthesis reaction. The success of any step of the surface modification is controlled by XPS analysis, contact angle measurements, SEM, and AFM observations. To evaluate the effect of PEG chain length, three diNH2PEGs (2000, 6000, and 20,000 D) of relative long polymer chain were employed as a spacer, expecting that a long flexible spacer could provide more conformational freedom for the collagen molecules and fibroblast reorganization to further cellular matrix formation. Human fibroblast cells were used as a model to evaluate the biological response of the collagen-immobilized PDMS surfaces. It is found that the earlier described biofunctionalization is one more road to improvement of the cellular interaction of PDMS, the last one being the best when PEG spacer with moderate chain length, namely of 6000 D, is used. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008 [source] Endothelial cell growth on silicon modified hydrogenated amorphous carbon thin filmsJOURNAL OF BIOMEDICAL MATERIALS RESEARCH, Issue 1 2008A. A. Ogwu Abstract The biological response of human microvascular endothelial cells (HMEC-1) seeded on Si-DLC films and on control surfaces was evaluated in terms of initial cell enhancement, growth, and cytotoxicity. The microstructure of the films was characterised by Raman spectroscopy and X-ray photoelectron spectroscopy. The effect of changes in microstructure, surface energy, surface electronic state, and electronic conduction, on the biological response of the films to endothelial cells was investigated. Endothelial cell adhesion and growth was found to be affected by changes in the microstructure of the films induced by silicon doping and thermal annealing. We observed a significant statistical difference in endothelial cell count between the as-deposited DLC and Si-DLC films using the one sample t -test at a p -value of 0.05. We also found a statistically significant difference between the adhesion of HMEC films on DLC and Si-DLC films at various annealing temperatures using the one-way ANOVA F statistic test at p < 0.05 and the post-hoc Tukey test. One sample t -test at p < 0.05 of MTT-assay results showed the endothelial cells to be viable when seeded on DLC/Si-DLC films. We suspect that the increased adhesion of endothelial cells induced by increasing the amount of silicon in the Si-DLC films is associated with the development of a suitable surface energy due to silicon addition, which neither favored cell denaturing nor preferential water spreading before cellular attachment on the film surface. The presence of an external positively charged dipole on the Si-DLC films confirmed by our Kelvin probe measurements is also expected to enhance the adhesion of endothelial cells that are well known to carry a negative charge. The Si-DLC films investigated hold potential promise as coatings for haemocompatible artificial implants. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2008 [source] Adapter protein CRKII signaling is involved in the rat pancreatic acini response to reactive oxygen speciesJOURNAL OF CELLULAR BIOCHEMISTRY, Issue 2 2006Alberto G. Andreolotti Abstract Recent studies demonstrate that reactive oxygen species (ROS) are important mediators of acute pancreatitis, whether induced experimentally or in necrotizing pancreatitis in humans; however, the cellular processes involved remain unclear. Adapter protein CrkII, plays a central role for convergence of cellular signals from different stimuli. Cholecystokinin (CCK), which induces pancreatitis, stimulates CrkII tyrosine phosphorylation and CrkII protein complexes, raising the possibility it can be important in the acinar cell responses to ROS. Therefore, our aim was to investigate whether CrkII signaling is involved in the biological response of rat pancreatic acini to H2O2 and the intracellular mediators implicated. Treatment of isolated rat pancreatic acini with H2O2 rapidly stimulates CrkII phosphorylation, measured as electrophoretic mobility shift and by using a phosphospecific antibody (pTyr221). Tyrosine kinase blocker B44 inhibits the higher phosphorylation state, demonstrating that it occurs mainly in tyrosine residues. H2O2 -induced CrkII phosphorylation is time- and concentration-dependent, showing maximal effect with 3 mM H2O2 at 5 min. The intracellular pathways induced by H2O2 leading to CrkII tyrosine phosphorylation do not involve PKC, intracellular calcium, PI3-K or the actin cytoskeleton integrity. ROS generation clearly promotes the formation of protein complex CrkII,PYK2. In conclusion, ROS clearly affect the key adapter protein CrkII signaling by two ways: stimulation of CkII phosphorylation and a functional consequence: formation of CrkII,protein complexes. Because of its central role in activating more distal pathways, CrkII might likely play an important role in the ability of ROS to induce pancreatic cellular injury and pancreatitis. J. Cell. Biochem. © 2005 Wiley-Liss, Inc. [source] Olfactory responses to steroids in an African mouth-brooding cichlid, Haplochromis burtoni(Günther)JOURNAL OF FISH BIOLOGY, Issue 3 2006T. B. Cole Underwater electro-olfactogram (EOG) recordings involving 150 steroids and eight prostaglandins were used to determine which of these potential odorants are detected by the olfactory organ of an African cichlid, Haplochromis burtoni. In initial EOG tests at 10,9 M, H. burtoni did not respond to unconjugated steroids or prostaglandins, but did respond to 17 conjugated steroids, 11 of which (17,-oestradiol-17,-glucuronide; 17,-oestradiol-3-sulphate; 17,-oestradiol-3,17,-disulphate; epiandrosteron-3,-sulphate; etiocholanolone-3,-glucuronide; testosterone-17,-sulphate; dehydroepiandrosterone-3,-sulphate; 5,-pregnan-3,-ol-20-one-3,-sulphate; 5,-pregnan-3,,17-diol-20-one-3,-glucuronide; 5,-pregnan-3,,17,21-triol-11,20-dione-3,-glucuronide; pregnenolone-3,-sulphate) were selected for EOG concentration-response, cross-adaptation and binary mixture tests. The EOG detection thresholds ranged from 10,11 to 10,9 M in all but one instance (female threshold to pregnenolone-3,-sulphate; 10,8 M), and males and females exhibited only minor differences in EOG threshold or response magnitude. Results of EOG cross-adaptation tests, which were supported by results of binary mixture tests, indicated that the response to the 11 steroid conjugates is mediated by five putative olfactory receptor mechanisms characterized by specificity for conjugate position and type: 3-sulphate, 17-sulphate, 3,17-disulphate, 3-glucuronide, 17-glucuronide. Although there is no evidence that H. burtoni releases, or exhibits biological response to, the steroids shown to be detected in this study, the present results are suggestive of a complex pheromone system utilizing steroid conjugates. [source] Oestrogen Receptors, Receptor Variants and Oestrogen Actions in the Hypothalamic-Pituitary AxisJOURNAL OF NEUROENDOCRINOLOGY, Issue 2 2002M. A. Shupnik Abstract Information on oestrogen action has grown exponentially in the past decade, and recent studies have begun to define the mechanism of ligand-dependent activation and cell-specific effects. Oestrogen-mediated gene transcription in a specific tissue depends on several factors, the most important of which is the presence of at least one of the two nuclear oestrogen receptor (ER) isoforms, ER, and ER,. The presence and levels of specific ER isoform variants, along with receptor coactivator, corepressor and integrator proteins, directly modulate overall nuclear ER activity. The structure of the ligand, including both physiological oestrogens and synthetic oestrogen receptor modulators, influences ER interactions with these other proteins and thus determines the biological response. Furthermore, peptide and neurotransmitter-stimulated intracellular signalling pathways activate specific enzyme cascades and may modify the receptors and their interacting proteins, resulting in either independent or ligand-enhanced ER-mediated responses. Finally, several rapid effects of oestrogen probably occur at the membrane through nongenomic pathways that may or may not require the same ER proteins that are found in the nucleus. This review concentrates on the pituitary-hypothalamic axis and the genomic effects of oestrogen, and discusses the current knowledge of each of these factors in determining oestrogen actions in the neuroendocrine system. [source] BDNF activated TrkB/IRR receptor chimera promotes survival of sympathetic neurons through Ras and PI-3 kinase signalingJOURNAL OF NEUROSCIENCE RESEARCH, Issue 2 2002Karen S. Kelly-Spratt Insulin receptor-related receptor (IRR) expression is tightly coupled to the nerve growth factor (NGF) receptor, TrkA, throughout development. Expression of both receptors is primarily localized to neural crest derived sensory and sympathetic neurons. In contrast to TrkA, however, the physiological ligand for IRR is unknown. To analyze the intracellular signaling and potential function of the orphan IRR in neurons, an adenovirus expressing a TrkB/IRR chimeric receptor was used to infect cultured mouse superior cervical ganglion neurons that normally require NGF for survival. Brain derived neurotrophic factor (BDNF)-activated TrkB/IRR induced neuronal survival. We utilized numerous receptor mutants in order to identify the intracellular domains of IRR necessary for signaling and neuron survival. Finally, we employed adenovirus encoding dominant negative forms of the extracellular signal-regulated kinase (ERK) signaling cascade to demonstrate that IRR, like TrkA, requires ras activation to promote neuron survival. Therefore, by use of the chimeric TrkB/IRR receptor, we have demonstrated the ability of IRR to elicit activation of signaling cascades resulting in a biological response in superior cervical ganglion (SCG) neurons. © 2002 Wiley-Liss, Inc. [source] Differential effects of static and dynamic compression on meniscal cell gene expressionJOURNAL OF ORTHOPAEDIC RESEARCH, Issue 6 2003Maureen L. Upton Abstract Cells of the meniscus are exposed to a wide range of time-varying mechanical stimuli that may regulate their metabolic activity in vivo. In this study, the biological response of the meniscus to compressive stimuli was evaluated in vitro, using a well-controlled explant culture system. Gene expression for relevant extracellular matrix proteins was quantified using real-time RT-PCR following a 24 h period of applied static (0.1 MPa compressive stress) or dynamic compression (0.08,0.16 MPa). Static and dynamic compression were found to differentially regulate mRNA levels for specific proteins of the extracellular matrix. Decreased mRNA levels were observed for decorin (,2.1 fold-difference) and type II collagen (,4.0 fold-difference) following 24 h of dynamic compression. Decorin mRNA levels also decreased following static compression (,4.5 fold-difference), as did mRNA levels for both types I (,3.3 fold-difference) and II collagen (,4.0 fold-difference). Following either static or dynamic compression, mRNA levels for aggrecan, biglycan and cytoskeletal proteins were unchanged. It is noteworthy that static compression was associated with a 2.6 fold-increase in mRNA levels for collagenase, or MMP-1, suggesting that the homeostatic balance between collagen biosynthesis and catabolism was altered by the mechanical stimuli. These findings demonstrate that the biosynthetic response of the meniscus to compression is regulated, in part, at the transcriptional level and that transcription of types I and II collagen as well as decorin may be regulated by common mechanical stimuli. © 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved. [source] Analysis of Heritability of Hormonal Responses to Alcohol in Twins: Beta-Endorphin as a Potential Biomarker of Genetic Risk for AlcoholismALCOHOLISM, Issue 3 2000J. C. Froehlich Background: Hormonal responses to alcohol have been reported to differ in subjects with and without a family history of alcoholism which suggests that alcohol-induced hormonal changes might be used to identify individuals who are at elevated genetic risk for developing alcoholism. However, before a biological response can be used as a marker of genetic risk for disease, it must first be demonstrated that the response is, in fact, heritable. The present study was designed to determine whether hormonal responses to alcohol are heritable. Methods: The adrenocorticotropic hormone (ACTH), beta-endorphin (,-E), cortisol (CORT), and prolactin (PRL) responses to alcohol were examined in male and female identical (monozygotic or MZ) and fraternal (dizygotic or DZ) twin pairs. Male subjects consumed 0.35g ethanol/kg body weight (BW) and female consumed 0.325 g ethanol/kg BW in each of two alcohol drinking sessions administered 1 hr apar (total dose of 0.7 g/kg BW in males and 0.65 g/kg BW in females). Plasma hormone content was analyzed in samples collected before (resting conditions) and at 15, 60, 75, 120, 180, and 240 min after onset of drinking. Hormonal responses to alcohol were examined with twin analyses using the TWINAN90 program. A separate analysis was performed for each of the four hormones. A subset of subjects from each zygosity was seen on two separate occasions to establish retest reliability. Heritability of hormonal responses to alcohol was estimated using the intraclass correlation approach before and after removing the contribution of covariates that have the potential of influencing the plasma levels of these hormones. Results: Resting plasma levels of all four hormones were within the expected range, and the ,-E, ACTH, and PRL responses to the alcohol challenge evidenced good test-retest reliability. Of the four hormones examined, the only one that showed significant heritability after alcohol drinking was ,-E. Heritability estimates were not altered for any of the four hormones after removal of the variance contributed by covariates, such as gender and age. Conclusions: Taken together with other recent findings, the results suggest that the ,-E response to alcohol may represent a new biomarker that can be used to identify individuals who are at elevated genetic risk for developing alcoholism. [source] WS10 Development of CALUX bioassay-based systems as instruments to detect hormones and contaminantsJOURNAL OF VETERINARY PHARMACOLOGY & THERAPEUTICS, Issue 2006A. BROUWER Objective Tremendous progress has been made in the ability to measure particular contaminants or veterinary drugs at very low concentrations. However, rare or previously unknown compounds, metabolites and mixtures are still presenting considerable analytical challenges, while this category in particular might be relevant in terms of food safety. In addition, the need for higher throughput screening strategies at lower costs also demands for methods in addition to chemical analysis. There is considerable development in methodology based on the interaction with bio-macromolecules or living cells or on a biological response in the exposed animal. The aim of this workshop is to provide an up-o-date and practical overview of the various analytical and biological strategies that are available to screen or detect (prior) exposure to drugs, contaminants and pollutants. [source] WS11 Comprehensive investigation of the transcriptomeJOURNAL OF VETERINARY PHARMACOLOGY & THERAPEUTICS, Issue 2006T. PINEAU Objective Tremendous progress has been made in the ability to measure particular contaminants or veterinary drugs at very low concentrations. However, rare or previously unknown compounds, metabolites and mixtures are still presenting considerable analytical challenges, while this category in particular might be relevant in terms of food safety. In addition, the need for higher throughput screening strategies at lower costs also demands for methods in addition to chemical analysis. There is considerable development in methodology based on the interaction with bio-macromolecules or living cells or on a biological response in the exposed animal. The aim of this workshop is to provide an up-o-date and practical overview of the various analytical and biological strategies that are available to screen or detect (prior) exposure to drugs, contaminants and pollutants. [source] Clinical review: Healing in gastrointestinal anastomoses, Part IMICROSURGERY, Issue 3 2006Sarah K. Thompson M.D. Gastrointestinal healing is a topic rarely reviewed in the literature, yet it is of paramount importance to the surgeon. Failure of anastomotic healing may lead to life-threatening complications, additional surgical procedures, increased length of stay, increased cost, long-term disability, and reduced quality of life for the patient. The goal of this article is to review the biological response to wounded tissue, to outline discrete differences between skin and gastrointestinal healing, to discuss local and systemic factors important to gastrointestinal healing, and to compare methods of measuring collagen content and strength of the newly formed anastomosis. Part II of this review will focus on techniques and therapies available to optimize anastomotic healing. © 2006 Wiley-Liss, Inc. Microsurgery, 2006. [source] Orthodontically stressed periodontium of transgenic mouse as a model for studying mechanical response in bone: The effect on the number of osteoblastsORTHODONTICS & CRANIOFACIAL RESEARCH, Issue 2 2000Dubravko Pavlin A better understanding of cellular and molecular mechanisms involved in response to mechanical stress is a prerequisite for future improvements in orthodontic treatment. To expand the application of molecular biology techniques in this area of research, we developed and characterized a mouse tooth movement model. The aim of this study was to biomechanically characterize this model and to evaluate the effect of orthodontic stress on the proliferation of periodontal osteoblasts. We used an orthodontic coil spring appliance with a low force/deflection rate, which produced an average force of 10,12 g. This design provided a predictable tipping movement of the molar with the center of rotation at the level of root apices. Histological observations of paradental tissues revealed a response favoring a fast onset of tooth movement and deposition of new osteoid starting after 3 days of treatment. The effect of treatment on the histomorpometric parameter of the number of osteoclasts per unit bone perimeter was determined after 1, 2, 3, 4, 6, and 12 days of treatment. Starting with day 2, the osteoblast number showed a modest but consistent increase in treated periodontal sites at all time-points, ranging from 14 to 39% and becoming significant only at day 6. Only a moderate increase in the number of osteoblasts in the areas of otherwise intense bone matrix synthesis suggests that, during bone formation, proliferation of cells has a smaller role compared to a marked increase in differentiation of individual cells. The mouse model, which allows for a controlled, reproducible, orthodontic mechanical loading, can be applied to both wild-type and transgenic animals and should enhance the research of the transduction of mechanical orthodontic signal into a biological response. [source] Selection in utero: A biological response to mass layoffsAMERICAN JOURNAL OF HUMAN BIOLOGY, Issue 3 2010Ralph Catalano Most research describing the biological response to unemployment appears appropriately motivated by clinical or public health concerns and focuses on death, disease, and medical care. We argue that expanding the work to include other outcomes could contribute to basic science. As an example, we use the response to mass layoffs to discriminate between two explanations of low ratios of male to female live births in stressed populations. One explanation asserts that ambient stressors reduce the ratio of males to females conceived. The other argues that the maternal stress response selects against males in utero. We show that selection in utero better explains the observed data. We conclude that human adaptation to the economic environment deserves scrutiny from a wider array of scientists than it now receives. Am. J. Hum. Biol., 2010. © 2009 Wiley-Liss, Inc. 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