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Bilayer Lipid Membrane (bilayer + lipid_membrane)
Selected AbstractsElectrochemical Elucidation of the Facilitated Ion Transport Across a Bilayer Lipid Membrane in the Presence of Neutral Carrier CompoundsELECTROANALYSIS, Issue 11 2010Jun Onishi Abstract The ion transport facilitated by neutral carrier compounds (valinomycin, nonactin) has been investigated by cyclic voltammetry in the several electrolyte solutions (KF, KCl, KBr, KNO3, KSCN, KClO4), and we demonstrated the effect of the counter anions on the facilitated transport of K+ from the viewpoint of electroneutrality. Voltammograms for the ion transport were generated at steady state and the current density between W1 and W2, jW1,W2, increased with the absolute value of the applied membrane potential, EW1,W2. Then, the magnitude of jW1,W2 at a certain EW1,W2 increased with the hydrophobicity of the counter anion. It was proved that the logarithm of |jW1,W2|at a certain EW1,W2 is almost proportional to the hydration energy of the counter anion. This indicates that not only K+ but also the counter anion distributes into the BLM. Therefore, the magnitude of jW1,W2 at a certain EW1,W2 increased with an increase of pH, because the hydroxide ion was served as a counter anion. Based on the variation of the zero-current potential in case of various asymmetrical ionic compositions, it is found that the amount of cation transport is much larger than that of anion transport. [source] Mediated Electron Transfer Across Supported Bilayer Lipid Membrane with TCNQ-Based Organometallic CompoundsELECTROANALYSIS, Issue 4 2010Meili Qu Abstract Supported bilayer lipid membrane (s-BLM) containing one-dimensional compound 1, TCNQ-based (TCNQ=7,7,8,8-tetracyanoquinodimethane) organometallic compound {(Cu2(,-Cl)(,-dppm)2)(,2 -TCNQ)},, was prepared and characterized on the self-assembled monolayer (SAM) of 1-octadecylmercaptan (C18H37SH) deposited onto Au electrode. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) results showed that the compound 1, dotted inside s-BLM, can act as mediator for electron transfer across the membrane. Two redox peaks and the charge-transfer resistance of 400,k, were observed for compound 1 inside s-BLM. The mechanism of the electron transfer across s-BLM by TCNQ is by electron hopping while TCNQ-based organometallic compound is by conducting. Further conclusion drawn from this finding is that the TCNQ-based organometallic compound embedded inside s-BLM exhibits excellent electron transfer ability than that of free TCNQ. This opens a new path for the development of s-BLM sensor and/or biosensor by incorporation with TCNQ-based organometallic compounds. [source] Study of the Ion Channel Behavior of Didodecyldimethylammonium Bromide Formed Bilayer Lipid Membrane Stimulated by PF6,CHINESE JOURNAL OF CHEMISTRY, Issue 1 2003Tong Yue-Hong Abstract Bilayer lipid membranes (BLM) formed from didodecyldimethylammonium bromide were made on the freshly exposed surface of a glassy carbon (GC) and were demonstrated by the ac impedance spectroscopy. The ion channels of membrane properties induced by PF6, were studied by the cyclic voltammetric methods. Experimental results indicated that the ion channel of BLM was open in the presence of the PF6, due to the interaction of PF6, with the BLM, while it was switched off in the absence of PF6,, Because the ion channel behavior was affected by the concentration of PF6,, a sensor for PF6, can be developed. [source] Mediated Electron Transfer Across Supported Bilayer Lipid Membrane with TCNQ-Based Organometallic CompoundsELECTROANALYSIS, Issue 4 2010Meili Qu Abstract Supported bilayer lipid membrane (s-BLM) containing one-dimensional compound 1, TCNQ-based (TCNQ=7,7,8,8-tetracyanoquinodimethane) organometallic compound {(Cu2(,-Cl)(,-dppm)2)(,2 -TCNQ)},, was prepared and characterized on the self-assembled monolayer (SAM) of 1-octadecylmercaptan (C18H37SH) deposited onto Au electrode. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) results showed that the compound 1, dotted inside s-BLM, can act as mediator for electron transfer across the membrane. Two redox peaks and the charge-transfer resistance of 400,k, were observed for compound 1 inside s-BLM. The mechanism of the electron transfer across s-BLM by TCNQ is by electron hopping while TCNQ-based organometallic compound is by conducting. Further conclusion drawn from this finding is that the TCNQ-based organometallic compound embedded inside s-BLM exhibits excellent electron transfer ability than that of free TCNQ. This opens a new path for the development of s-BLM sensor and/or biosensor by incorporation with TCNQ-based organometallic compounds. [source] Voltammetric Elucidation of Ion Transfer Through an Extremely Thin MembraneELECTROANALYSIS, Issue 9 2004Nobuyuki Ichieda Abstract Digital simulation of the cyclic voltammogram for the ion transfer through a liquid membrane of thickness from 1,mm to 10,nm was performed. The magnitude of current and the shape of the voltammogram simulated for extremely thin membrane (10,nm thick) were similar to those observed experimentally with a bilayer lipid membrane, BLM, of about 10,nm in thick, when the diffusion coefficient of an ion in the BLM was assumed to be extraordinary small (10,13 to 10,14,cm2 s,1). [source] A Self-assembly Route for Double Bilayer Lipid Membrane FormationCHEMPHYSCHEM, Issue 3 2010Xiaojun Han Dr. Biomembranes: A new method of forming double bilayer lipid membranes uses NHS/EDC chemistry to link adjacent bilayers, both of which remain fluid. This approach provides a novel platform for the study of biomembranes, in which the components of the upper membrane are shielded from the solid substrate by a second membrane (see figure); and for studying more complex membrane protein systems which span double lipid bilayers. [source] | ||||||||||