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Young Ones (young + ones)
Selected AbstractsFree radical generation and oxidative stress with ageing and exercise: Differential effects in the myocardium and liverACTA PHYSIOLOGICA, Issue 4 2000Bejma Reactive oxygen species and other oxidants are implicated in the mechanisms of biological ageing and exercise-induced tissue damage. The present study examined the effects of ageing and an acute bout of exercise on intracellular oxidant generation, lipid peroxidation, protein oxidation and glutathione (GSH) status in the heart and liver of young adult (8 month, N=24) and old (24 month, N=24) male Fischer 344 rats. Young rats ran on treadmill at 25 m min,1, 5% grade until exhaustion (55.4 ± 2.7 min), whereas old rats ran at 15 m min,1, 5% until exhaustion (58.0 ± 2.7 min). Rate of dichlorofluorescin (DCFH) oxidation, an indication of intracellular oxidant production, was significantly higher in the homogenates of aged heart and liver compared with their young counterparts. In the isolated heart and liver mitochondria, ageing increased oxidant production by 29 and 32% (P < 0.05), respectively. Acute exercise increased oxidant production in the aged heart but not in the liver. When nicodinamide dinucleotide phosphate (reduced), adenosine diphosphate and Fe3+ were included in the assay, DCFH oxidation rate was 47 and 34% higher (P < 0.05) in the aged heart and liver homogenates, respectively, than the young ones. The age differences in the induced state reached 83 and 140% (P < 0.01) in isolated heart and liver mitochondria, respectively. Lipid peroxidation was increased in the aged liver and exercised aged heart, whereas protein carbonyl content was elevated only in the aged heart (P < 0.05). Although our data using DCFH method probably underestimated cellular oxidant production because of time delay and antioxidant competition, it is clear that oxidative stress was enhanced in both heart and liver with old age. Furthermore, aged myocardium showed greater susceptibility to oxidative stress after heavy exercise. [source] Tyatya Volcano, southwestern Kuril arc: Recent eruptive activity inferred from widespread tephraISLAND ARC, Issue 4 2002MITSUHIRO NAKAGAWA Abstract Tyatya Volcano, situated in Kunashir Island at the southwestern end of Kuril Islands, is a large composite stratovolcano and one of the most active volcanoes in the Kuril arc. The volcanic edifice can be divided into the old and the young ones, which are composed of rocks of distinct magma types, low- and medium-K series, respectively. The young volcano has a summit caldera with a central cone. Recent eruptions have occurred at the central cone and at the flank vents of the young volcano. We found several distal ash layers at the volcano and identified their ages and sources, that is, tephras of ad 1856, ad 1739, ad 1694 and ca 1 Ka derived from three volcanoes of Hokkaido, Japan, and caad 969 from Baitoushan Volcano of China/North Korea. These could provide good time markers to reveal the eruptive history of the central cone, which had continued intermittently with Strombolian eruptions and lava flow effusions since before 1 Ka. Relatively explosive eruptions have occurred three times at the cone during the past 1000 years. We revealed that, topographically, the youngest lava flows from the cone are covered not by the tephra of ad 1739 but by that of ad 1856. This evidence, together with a report of dense smoke rising from the summit in ad 1812, suggests that the latest major eruption with lava effusion from the central cone occurred in this year. In 1973, after a long period of dormancy, short-lived phreatomagmatic eruptions began to occur from fissure vents at the northern flank of the young volcano. This was followed by large eruptions of Strombolian to sub-Plinian types occurring from several craters at the southern flank. The 1973 activity is evaluated as Volcanic Explosivity Index = 4 (approximately 0.2 km3), the largest eruption during the 20th century in the southwestern Kuril arc. The rocks of the central cone are strongly porphyritic basalt and basaltic andesite, whereas the 1973 scoria is aphyric basalt, suggesting that magma feeding systems are definitely different between the summit and flank eruptions. [source] Aging induces cardiac diastolic dysfunction, oxidative stress, accumulation of advanced glycation endproducts and protein modificationAGING CELL, Issue 2 2005Shi-Yan Li Summary Evidence suggests that aging, per se, is a major risk factor for cardiac dysfunction. Oxidative modification of cardiac proteins by non-enzymatic glycation, i.e. advanced glycation endproducts (AGEs), has been implicated as a causal factor in the aging process. This study was designed to examine the role of aging on cardiomyocyte contractile function, cardiac protein oxidation and oxidative modification. Mechanical properties were evaluated in ventricular myocytes from young (2-month) and aged (24,26-month) mice using a MyoCam® system. The mechanical indices evaluated were peak shortening (PS), time-to-PS (TPS), time-to-90% relengthening (TR90) and maximal velocity of shortening/relengthening (± dL/dt). Oxidative stress and protein damage were evaluated by glutathione and glutathione disulfide (GSH/GSSG) ratio and protein carbonyl content, respectively. Activation of NAD(P)H oxidase was determined by immunoblotting. Aged myocytes displayed a larger cell cross-sectional area, prolonged TR90, and normal PS, ± dL/dt and TPS compared with young myocytes. Aged myocytes were less tolerant of high stimulus frequency (from 0.1 to 5 Hz) compared with young myocytes. Oxidative stress and protein oxidative damage were both elevated in the aging group associated with significantly enhanced p47phox but not gp91phox expression. In addition, level of cardiac AGEs was ,2.5-fold higher in aged hearts than young ones determined by AGEs-ELISA. A group of proteins with a molecular range between 50 and 75 kDa with pI of 4,7 was distinctively modified in aged heart using one- or two-dimension SDS gel electrophoresis analysis. These data demonstrate cardiac diastolic dysfunction and reduced stress tolerance in aged cardiac myocytes, which may be associated with enhanced cardiac oxidative damage, level of AGEs and protein modification by AGEs. [source] Litterfall of epiphytic macrolichens in Nothofagus forests of northern Patagonia, Argentina: Relation to stand age and precipitationAUSTRAL ECOLOGY, Issue 3 2006MAYRA S. CALDIZ Abstract: The objective of this study was to analyse how stand age and precipitation influence abundance and diversity of epiphytic macrolichens in southern beech Nothofagus forests, estimated by lichen litter sampling. Five sites of Nothofagus dombeyi (Mirbel) Oersted were selected in Nahuel Huapi National Park, Argentina. At each site, lichen fragments from the forest floor were collected at 12.5 m2 plots in pairs of young and mature N. dombeyi forest. Additionally, two sites with multi-aged subalpine Nothofagus pumilio (Poepp. et Endl.) Krasser forest were investigated in a similar manner. Average litterfall biomass per stand varied from less than 1 kg ha,1 in a young low-precipitation stand to a maximum of 20 kg ha,1 in a mature high-precipitation stand. In places with higher precipitation, litterfall biomass in N. dombeyi forest was considerably higher in old stands as compared with young ones. In places with less than 2000 mm of precipitation, differences in biomass were less pronounced. Old humid stands contained about twice as many taxa in the litter as old low-precipitation stands and young stands in general. Mature stands in low-precipitation sites only contained 17% of the litter biomass as compared with mature stands in high-precipitation sites. Epiphytic lichen composition changed from predominating fruticose lichens (Usnea spp. and Protousnea spp.) in low-precipitation stands to Pseudocyphellaria spp., Nephroma spp. and other foliose lichens, in the high-precipitation stands. There were no clear differences in the proportion of fruticose and foliose lichens between young and old stands. Fruticose lichens dominated litter biomass in both N. pumilio sites. [source] | ||||||||||