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Wheat Chromosomes (wheat + chromosome)

Distribution by Scientific Domains

Life Sciences	100%

Selected Abstracts

Molecular cytogenetic analysis of a durum wheat ×Thinopyrum distichum hybrid used as a new source of resistance to Fusarium head blight in the greenhouse

PLANT BREEDING, Issue 5 2001
Q. Chen
Abstract Fusarium head blight (FHB, scab), caused by Fusarium graminearum Schwabe, is a serious and damaging disease of wheat. Although some hexaploid wheat lines express a good level of resistance to FHB, the resistance available in hexaploid wheat has not yet been transferred to durum wheat. A germplasm collection of Triticum durum× alien hybrid lines was tested as a potential source of resistance to FHB under controlled conditions. Their FHB reaction was evaluated in three tests against conidial suspensions of three strains of F. graminearum at the flowering stage. Two T. durum×Thinopyrum distichum hybrid lines, ,AFR4' and ,AFR5,, expressed a significantly higher level of resistance to the spread of FHB than other durum-alien hybrid lines and a resistant common wheat line ,Nyu-Bay'. Genomic in situ hybridization using total genomic DNA from alien grass species demonstrated that ,AFR5' had 13 or 14 alien genome chromosomes plus 27 or 28 wheat chromosomes, while ,AFR4' had 22 alien genome and 28 wheat chromosomes. All of the alien chromosomes present in these two lines belonged to the J genome. ,AFR5' is likely to be more useful as a source of FHB resistance than ,AFR4' because of its relatively normal meiotic behaviour, high fertility and fewer number of alien chromosomes. ,AFR5' shows good potential as a source for transferring FHB resistance gene into wheat. The development of T. durum addition lines carrying resistance genes from ,AFR5' is underway. [source]

A microsatellite marker linked to leaf rust resistance transferred from Aegilops triuncialis into hexaploid wheat

PLANT BREEDING, Issue 3 2001
M. Aghaee-Sarbarzeh
Abstract Aegilops triuncialis (UUCC) is an excellent source of resistance to various wheat diseases, including leaf rust. Leaf rust-resistant derivatives from a cross of a highly susceptible Triticum aestivum cv.,WL711' as the recurrent parent and Ae. triuncialis Ace.3549 as the donor and with and without a pair of acrocentric chromosomes were used for molecular tagging. The use of a set of sequence tagged microsatellite (STMS) markers already mapped to different wheat chromosomes unequivocally indicated that STMS marker gwm368 of chromosome 4BS was tightly linked to the Ae. triuncialis leaf rust resistance gene transferred to wheat. The presence of the Ae. Triuncialis -specific STMS gwm368 homoeoallele along with the non-polymorphic 4BS allele in the rust-resistant derivatives with and without the acrocentric chromosome indicates that the resistance has been transferred from the acrocentric chromosome to either the A or the D genome of wheat. This alien leaf rust resistance gene has been temporarily named as LrTr. [source]

Molecular cytogenetic characterization of Thinopyrum genomes conferring perennial growth habit in wheat- Thinopyrum amphiploids

PLANT BREEDING, Issue 1 2001
X. Cai
Abstract Seven wheat- Thinopyrum amphiploids, AT 3425, AgCs, PI 550710, PI 550711, PI 550712, PI 550713 and PI 550714, were evaluated for perennial growth habit in the field. Three of them, AgCs, AT 3425, and PI 550713, were identified as perennials. Fluorescent genomic in situ hybridization (FGISH) patterns of mitotic chromosomes indicated that AgCs had seven pairs of Thinopyrum chromosomes and 21 pairs of wheat chromosomes. PI 550713 and AT 3425 showed similar FGISH patterns of mitotic chromosomes with three pairs of wheat- Thinopyrum translocated chromosomes, seven pairs of Thinopyrum chromosomes, and 18 pairs of wheat chromosomes. Thinopyrum chromosome pairing in the Fi hybrid of AT 3425 with AgCs demonstrated differences between Thinopyrum genomes in these two amphiploids. Based on chromosome constitutions, pairing and reported pedigrees, AgCs and AT 3425 were identified as a wheat- Thinopyrum elongatum amphiploid and partial wheat- Thinopyrum ponticum amphiploid, respectively. Chromosome pairing in the F1 hybrid between AT 3425 and PI 550713 revealed that these two amphiploids contained the same Thinopyrum genome. Two different Thinopyrum genomes conferring perennial growth habit were identified from the perennial amphiploids and characterized cytogenetically. [source]

Genetic diversity in a collection of old and new bread wheat cultivars from Iran as revealed by simple sequence repeat-based analysis

ANNALS OF APPLIED BIOLOGY, Issue 1 2009
S.A. Mohammadi
Abstract Genetic diversity in a collection of 70 bread wheat (Triticum aestivum) genotypes was studied using 73 microsatellite [simple sequence repeat (SSR)] loci evenly spaced on wheat chromosomes. A total of 592 alleles with an average of 8.53 allele/locus were detected, of which 185 (31.25%) occurred only in a specific group of genotypes. A set of SSR markers consisted of 22 loci with polymorphic information content values of 0.80 or higher were selected for rapid fingerprinting of many genotypes. Average of gene diversity was 0.74 ± 0.017, and significant difference between observed and maximum theoretical values of gene diversity in the analysed SSR loci was obtained using a paired t -test. Genetic distance-based clustering methods including unweighted pair group method with arithmetic average and neighbour joining (NJ) were used for grouping of genotypes. The resulted dendrogram based on NJ and number of differences coefficient hinted of the existence of three groups. This grouping was in agreement with the pedigree information and confirmed by high within-group bootstrap value. A comparatively higher genetic diversity in the studied wheat collection as revealed by presence of high allelic diversity and large number of specific alleles could be utilised in development of new cultivars with desired characteristics. [source]