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Water Transport Capacity (water + transport_capacity)
Selected AbstractsEffects of agrin on the expression and distribution of the water channel protein aquaporin-4 and volume regulation in cultured astrocytesEUROPEAN JOURNAL OF NEUROSCIENCE, Issue 8 2007Susan Noell Abstract Agrin is a heparan sulfate proteoglycan of the extracellular matrix and is known for organizing the postsynaptic differentiation of the neuromuscular junction. Increasing evidence also suggests roles for agrin in the developing CNS, including the formation and maintenance of the blood,brain barrier. Here we describe effects of agrin on the expression and distribution of the water channel protein aquaporin-4 (AQP4) and on the swelling capacity of cultured astrocytes of newborn mice. If astrocytes were cultured on a substrate containing poly dl -ornithine, anti-AQP4 immunoreactivity was evenly and diffusely distributed. If, however, astrocytes were cultured in the presence of agrin-conditioned medium, we observed an increase in the intensity of AQP4-specific membrane-associated staining. Freeze-fracture studies revealed a clustering of orthogonal arrays of particles, representing a structural equivalent of AQP4, when exogenous agrin was present in the astrocyte cultures. Neuronal and non-neuronal agrin isoforms (agrin A0B0 and agrin A4B8, respectively) were able to induce membrane-associated AQP4 staining. Water transport capacity as well as the density of orthogonal arrays of intramembranous particles was increased in astrocytes cultured with the neuronal agrin isoform A4B8, but not with the endothelial and meningeal isoform A0B0. RT-PCR demonstrated that agrin A4B8 increased the level of the M23 splice variant of AQP4 and decreased the level of the M1 splice variant of AQP4. Implications for the regulation and maintenance of the blood,brain barrier including oedema formation under pathological conditions are discussed. [source] Height-related trends in leaf xylem anatomy and shoot hydraulic characteristics in a tall conifer: safety versus efficiency in water transportNEW PHYTOLOGIST, Issue 1 2008D. R. Woodruff Summary ,,Hydraulic vulnerability of Douglas-fir (Pseudotsuga menziesii) branchlets decreases with height, allowing shoots at greater height to maintain hydraulic conductance (Kshoot) at more negative leaf water potentials (,l). ,,To determine the basis for this trend shoot hydraulic and tracheid anatomical properties of foliage from the tops of Douglas-fir trees were analysed along a height gradient from 5 to 55 m. ,,Values of ,l at which Kshoot was substantially reduced, declined with height by 0.012 Mpa m,1. Maximum Kshoot was reduced by 0.082 mmol m,2 MPa,1 s,1 for every 1 m increase in height. Total tracheid lumen area per needle cross-section, hydraulic mean diameter of leaf tracheid lumens, total number of tracheids per needle cross-section and leaf tracheid length decreased with height by 18.4 µm2 m,1, 0.029 µm m,1, 0.42 m,1 and 5.3 µm m,1, respectively. Tracheid thickness-to-span ratio (tw/b)2 increased with height by 1.04 × 10,3 m,1 and pit number per tracheid decreased with height by 0.07 m,1. ,,Leaf anatomical adjustments that enhanced the ability to cope with vertical gradients of increasing xylem tension were attained at the expense of reduced water transport capacity and efficiency, possibly contributing to height-related decline in growth of Douglas fir. [source] Functional coordination between leaf gas exchange and vulnerability to xylem cavitation in temperate forest treesPLANT CELL & ENVIRONMENT, Issue 4 2006HAFIZ MAHERALI ABSTRACT We examined functional coordination among stem and root vulnerability to xylem cavitation, plant water transport characteristics and leaf traits in 14 co-occurring temperate tree species. Relationships were evaluated using both traditional cross-species correlations and phylogenetically independent contrast (PIC) correlations. For stems, the xylem tension at which 50% of hydraulic conductivity was lost (,50) was positively associated (P < 0.001) with specific conductivity (KS) and with mean hydraulically weighted xylem conduit diameter (Dh-w), but was only marginally (P = 0.06) associated with leaf specific conductivity (KL). The PIC correlation for each of these relationships, however, was not statistically significant. There was also no relationship between root ,50 and root KS in either cross-species or PIC analysis. Photosynthetic rate (A) and stomatal conductance (gs) were strongly and positively correlated with root ,50 in the cross-species analysis (P < 0.001), a relationship that was robust to phylogenetic correction (P < 0.01). A and gs were also positively correlated with stem ,50 in the cross-species analysis (P = 0.02 and 0.10, respectively). However, only A was associated with stem ,50 in the PIC analysis (P = 0.04). Although the relationship between vulnerability to cavitation and xylem conductivity traits within specific organs (i.e. stems and roots) was weak, the strong correlation between gs and root ,50 across species suggests that there is a trade-off between vulnerability to cavitation and water transport capacity at the whole-plant level. Our results were therefore consistent with the expectation of coordination between vulnerability to xylem cavitation and the regulation of stomatal conductance, and highlight the potential physiological and evolutionary significance of root hydraulic properties in controlling interspecific variation in leaf function. [source] Hydraulic properties and freezing-induced cavitation in sympatric evergreen and deciduous oaks with contrasting habitatsPLANT CELL & ENVIRONMENT, Issue 12 2001J. Cavender-Bares Abstract We investigated the hydraulic properties in relation to soil moisture, leaf habit, and phylogenetic lineage of 17 species of oaks (Quercus) that occur sympatrically in northern central Florida (USA). Leaf area per shoot increased and Huber values (ratio of sapwood area to leaf area) decreased with increasing soil moisture of species' habitats. As a result, maximum hydraulic conductance and maximum transpiration were positively correlated with mean soil moisture when calculated on a sapwood area basis, but not when calculated on a leaf area basis. This reveals the important role that changes in allometry among closely related species can play in co-ordinating water transport capacity with soil water availability. There were significant differences in specific conductivity between species, but these differences were not explained by leaf habit or by evolutionary lineage. However, white oaks had significantly smaller average vessel diameters than red oaks or live oaks. Due to their lower Huber values, maximum leaf specific conductivity (KL) was higher in evergreen species than in deciduous species and higher in live oaks than in red oaks or white oaks. There were large differences between species and between evolutionary lineages in freeze,thaw-induced embolism. Deciduous species, on average, showed greater vulnerability to freezing than evergreen species. This result is strongly influenced by evolutionary lineage. Specifically, white oaks, which are all deciduous, had significantly higher vulnerability to freezing than live oaks (all evergreen) and red oaks, which include both evergreen and deciduous species. These results highlight the importance of taking evolutionary lineage into account in comparative physiological studies. [source] | ||||||||||