Water Quality Parameters (water + quality_parameter)

Distribution by Scientific Domains


Selected Abstracts


Fisheries of two tropical lagoons in Ghana, West Africa

FISHERIES MANAGEMENT & ECOLOGY, Issue 6 2004
H. R. Dankwa
Abstract, The fisheries of two coastal lagoons, Keta and Songor, were studied as part of Ghana Coastal Wetlands Management Project (GCWMP) aimed at sustainable exploitation of wetland resources. Fish samples were obtained with seine nets and cast net as well as from local fishermen. Water quality parameters (pH, dissolved oxygen, temperature and turbidity) were similar in the two lagoons, except for salinity, which was significantly different (P < 0.001). Despite their close geographical proximity, the two lagoons supported different fish assemblages with the blackchin tilapia, Sarotherodon melanotheron Rüppell, and the redchin tilapia, Tilapia guineensis (Bleeker), being the most important commercial fishes in both lagoons. The number of individuals for each species in Songor Lagoon were far more abundant, with densities several orders of magnitude higher than in Keta Lagoon. However, both species were significantly larger (P < 0.01) in the latter [15,121 and 25,157 mm standard length (SL)] than in the former lagoon (30,102 and 15,95 mm SL) for S. melanotheron and T. guineensis respectively. Over-fishing, use of small-size mesh nets, limited mixing of marine and fresh water were some of the factors limiting fish production in both lagoons. [source]


The occurrence of Campylobacter in river water and waterfowl within a watershed in southern Ontario, Canada

JOURNAL OF APPLIED MICROBIOLOGY, Issue 3 2010
M.I. Van Dyke
Abstract Aims:, Quantitative PCR and a culture method were used to investigate Campylobacter occurrence over 3 years in a watershed located in southern Ontario, Canada that is used as a source of drinking water. Methods and Results:, Direct DNA extraction from river water followed by quantitative PCR analysis detected thermophilic campylobacters at low concentrations (<130 cells 100 ml,1) in 57,79% of samples taken from five locations. By comparison, a culture-based method detected Campylobacter in 0,23% of samples. Water quality parameters such as total Escherichia coli were not highly correlated with Campylobacter levels, although higher pathogen concentrations were observed at colder water temperatures (<10°C). Strains isolated from river water were primarily nalidixic acid-susceptible Campylobacter lari, and selected isolates were identified as Campylobacter lari ssp. concheus. Campylobacter from wild birds (seagulls, ducks and geese) were detected at a similar rate using PCR (32%) and culture-based (29%) methods, and although Campylobacter jejuni was isolated most frequently, C. lari ssp. concheus was also detected. Conclusions:,Campylobacter were frequently detected at low concentrations in the watershed. Higher prevalence rates using quantitative PCR was likely because of the formation of viable but nonculturable cells and low recovery of the culture method. In addition to animal and human waste, waterfowl can be an important contributor of Campylobacter in the environment. Significance and Impact of the Study:, Results of this study show that Campylobacter in surface water can be an important vector for human disease transmission and that method selection is important in determining pathogen occurrence in a water environment. [source]


Dietary protein level and natural food management in the culture of blue (Litopenaeus stylirostris) and white shrimp (Litopenaeus vannamei) in microcosms

AQUACULTURE NUTRITION, Issue 3 2003
L.R. Martinez-Cordova
Abstract The effect of dietary protein level and natural food management on the production parameters of blue and white shrimp, as well as on water quality, was evaluated in a microcosms system (plastic pools simulating aquaculture ponds). Two experimental trials were carried out in the facilities of DICTUS, University of Sonora, Northwest México. Treatment with low protein diet (LP) consisted of a low protein input (diet with 250 g kg,1 crude protein) through the culture period; treatment with high protein diet (HP) consisted of a high protein input (diet with 400 g kg,1 crude protein) through the trial, and finally treatment VP consisted of an adjustment of protein input (diets with 250, 350 or 400 g kg,1 crude protein), depending on the abundance of biota (zooplankton and benthos) in the system. Each species responded differently to the treatments. For blue shrimp, low protein input resulted in the lowest final body weight (12.9 ± 0.6 g) and biomass (696.0 g pool,1). Survival and feed conversion ratio were similar in the three treatments. For white shrimp, the best growth, biomass and food conversion ratio were obtained in the low protein input treatment. Water quality parameters such as nitrate, ammonia and organic matter during the two trials, were better for LP and VP treatments. White shrimp seems to have lower protein requirements than blue shrimp. For the blue shrimp culture, adjusting protein input according to natural food abundance (zooplankton and benthos) in the system, seems to be advantageous because of the possibility of getting a production similar to that obtained with a high protein input through the farming period, but at lower feed cost, and with a lower environmental impact. It is concluded that a high protein input through the whole farming period is not the best feeding strategy for any of the two species. [source]


Effects of selective harvesting and claw ablation of all-male freshwater prawn (Macrobrachium rosenbergii ) on water quality, production and economics in polyculture ponds

AQUACULTURE RESEARCH, Issue 10 2010
Sheikh Md.
Abstract The effects of selective harvesting (SH) and claw ablation (CA) of blue-clawed (BC) prawns on an all-male freshwater prawn,finfish polyculture system were compared with control (Co) in quadruplicate. Ponds were stocked with all-male freshwater prawn Macrobrachium rosenbergii, silver carp Hypophthalmichthys molitrix, catla Catla catla and mola Amblypharyngodon mola at 12 000, 2000, 500 and 20 000 ha,1 respectively. Prawns were fed with pelleted feed. Ponds were fertilized regularly with urea, triple super phosphate and cow-dung. SH of BC prawns in treatment SH and CA in treatment CA started on the 60th day during a 137-day culture and continued at 15-day intervals until the final harvest. Water quality parameters and plankton abundance did not vary significantly (P>0.05) among the treatments. Treatment SH resulted in a higher (P<0.05) net production of freshwater prawn (437 kg ha,1), with better survival and mean weight, followed by CA (354 kg ha,1) and Co (322 kg ha,1). The combined net production of prawn plus finfish was also higher in SH (1244 kg ha,1) as compared with CA (1161 kg ha,1) and Co (1137 kg ha,1), although the finfish production did not differ significantly. The periodic SH of BC prawns showed a better economic return with a BCR of 1.71. [source]


A universal index formula suitable to multiparameter water quality evaluation

NUMERICAL METHODS FOR PARTIAL DIFFERENTIAL EQUATIONS, Issue 3 2004
Lihong Peng
Abstract Based on hypothesis of "base value" of water quality parameter, a universal index suitable to multi-parameter water quality evaluation was presented, as the monitoring values of parameters in the water quality index formula in logarithm function form were replaced by their relative values, and optimizing the parameters of different indices in the formula was carried out by Genetic Algorithms. Each index of water quality can be weighted into comprehensive index by compromise active function. The correctitude of formula was verified by using this method to asses the water quality states of many spots. The formula has shown its simplicity of calculation, practicability, generality, comparability and objectivity. © 2004 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq 20: 368,373, 2004 [source]


A performance comparison of individual and combined treatment modules for water recycling

ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY, Issue 4 2005
Stuart Khan
Abstract An Advanced Water Recycling Demonstration Plant (AWRDP) was commissioned and constructed by the Queensland State Government in Australia. The AWRDP was used to study the effectiveness of a variety of treatment processes in the upgrading of municipal wastewater for water recycling applications. The AWRDP consists of eight modules, each housing an individual specific treatment process. These processes are flocculation, dissolved air flotation, dual media filtration, ozonation, biological activated carbon adsorption, microfiltration, reverse osmosis, and ultraviolet disinfection. The individual performances of the treatment processes were determined, as well as their interdependence in series. A range of chemical water quality parameters were investigated. The study provides a broad process comparison on the basis of an important catalogue of these key parameters. This will be valuable in the selection and optimization of treatment processes trains in full-scale water recycling applications. © 2005 American Institute of Chemical Engineers Environ Prog, 2005 [source]


Comparison of hardness- and chloride-regulated acute effects of sodium sulfate on two freshwater crustaceans

ENVIRONMENTAL TOXICOLOGY & CHEMISTRY, Issue 4 2007
David John Soucek
Abstract Based on previous observations that hardness (and potentially chloride) influences sodium sulfate toxicity, the objective of the current study was to quantify the influence of both chloride and water hardness on acute toxicity to Hyalella azteca and Ceriodaphnia dubia. In addition, observed toxicity data from the present study were compared to toxicity predictions by the salinity/toxicity relationship (STR) model. Hardness had a strong influence on sulfate toxicity that was similar for both crustaceans, and nearly identical median lethal concentration (LC50)/hardness slopes were observed for the two species over the tested range. Chloride had a strong but variable influence on sulfate acute toxicity, depending on the species tested and the concentration range. At lower chloride concentrations, LC50s for H. azteca strongly were correlated positively with chloride concentration, although chloride did not affect the toxicity of sodium sulfate to C. dubia. The opposite trend was observed over the higher range of chloride concentrations where there was a negative correlation between chloride concentration and sulfate LC50 for both species. The widely ranging values for both species and a high correlation between LC50s in terms of sulfate and conductivity suggested that, whether based on sulfate, conductivity, or total dissolved solids (TDS), attempts at water quality standard development should incorporate the fact that water quality parameters such as hardness and chloride strongly influence the toxicity of high TDS solutions. The STR model predicted toxicity to C. dubia relatively well when chloride was variable and hardness fixed at approximately 100 mg/L; however, the model did not account for the protective effect of hardness on major ion/TDS toxicity. [source]


Application of Direct Push Methods to Investigate Uranium Distribution in an Alluvial Aquifer

GROUND WATER MONITORING & REMEDIATION, Issue 4 2009
Wesley McCall
The U.S. EPA 2000 Radionuclide Rule established a maximum contaminant level (MCL) for uranium of 30 µg/L. Many small community water supplies are struggling to comply with this new regulation. At one such community, direct push (DP) methods were applied to obtain hydraulic profiling tool (HPT) logs and install small diameter wells in a section of alluvial deposits located along the Platte River. This work was conducted to evaluate potential sources of elevated uranium in the Clarks, Nebraska drinking water supply. HPT logs were used to understand the hydrostratigraphy of a portion of the aquifer and guide placement of small diameter wells at selected depth intervals. Low-flow sampling of the wells provided water quality parameters and samples for analysis to study the distribution of uranium and variations in aquifer chemistry. Contrary to expectations, the aquifer chemistry revealed that uranium was being mobilized under anoxic and reducing conditions. Review of the test well and new public water supply well construction details revealed that filter packs extended significantly above the screened intervals of the wells. These filter packs were providing a conduit for the movement of groundwater with elevated concentrations of uranium into the supply wells and the community drinking water supply. The methods applied and lessons learned here may be useful for the assessment of unconsolidated aquifers for uranium, arsenic, and many other drinking water supply contaminants. [source]


Processes governing river water quality identified by principal component analysis

HYDROLOGICAL PROCESSES, Issue 16 2002
I. Haag
Abstract The present study demonstrates the usefulness of principal component analysis in condensing and interpreting multivariate time-series of water quality data. In a case study the water quality system of the lock-regulated part of the River Neckar (Germany) was analysed, with special emphasis on the oxygen budget. Pooled data of ten water quality parameters and discharge, which had been determined at six stations along a 200 km reach of the river between the years 1993 and 1998, were subjected to principal component analysis. The analysis yielded four stable principal components, explaining 72% of the total variance of the 11 parameters. The four components could be interpreted confidently in terms of underlying processes: biological activity, dilution by high discharge, seasonal effects and the influence of wastewater. From analysing the data of single stations separately, these processes were found to be active throughout the complete reach. Considering the oxygen budget of the river, the variance of biological activity, representing the counteracting processes of primary production and microbial degradation, was found to be most important. This principal component explained 79% of the observed variance of oxygen saturation. In contrast, the analysis of a reduced data set from the 1970s showed that oxygen saturation was then dominated by discharge and temperature variations. The findings indicate that the oxygen budget used to be governed directly by the emission of degradable matter, whereas nowadays eutrophication is most important for extreme oxygen concentrations. Therefore, controlling eutrophication has to be the primary goal, in order to mitigate the rare episodes of pronounced oxygen over- and undersaturation in the future. Copyright © 2002 John Wiley & Sons, Ltd. [source]


Some aspects of the biology of the stargazer mountain catfish, Amphilius uranoscopus (pfeffer); (Siluriformes: Amphiliidae) indigenous to Kenya streams

AFRICAN JOURNAL OF ECOLOGY, Issue 4 2009
Charles C. Ngugi
Abstract A study on some biological parameters of the mountain catfish, Amphilius uranoscopus Pfeffer 1889 (Silurifomes: Amphiliidae), was carried out in the Thego stream on the slopes of Mount Kenya from February to December 2002. Physical and chemical profiles of the Thego show that the water quality parameters is typical of high altitude streams with temperatures rarely exceeding 18°C, DO ranging from 7.9 to 8.2 mg l,1 and relatively high conductivity (97,137 ,S cm,1) typical of perturbed lotic environments. A total of 1010 fish were caught by an electro-fisher, with sizes ranging between 8 and 24 cm fork length. The population structure had a unimodal distribution with maxima at 14,16 cm. The length,weight relationship showed relatively narrow range in the slope ranging from 2.61 in April to 2.98 in February 2002, thereby suggesting isometric growth pattern. The fitted growth pattern of A. uranoscopus showed an asymptotic length (L,) of 28.5 cm and a growth curvature (K) of 0.56 year,1 resulting in an estimated natural mortality coefficient (M) of 0.90 year,1. The Fulton's condition factor (K) was also relatively stable with a peak in April (0.92 ± 0.21) and lowest value in June (0.86 ± 0.10). As A. uranoscopus is not under commercial exploitation, the seemingly depressed population is possibly attributed to the introduced exotic rainbow trout that heavily predates on the species and environmental perturbations arising from changes in land use. The implications of such changes on A. uranoscopus are discussed. Résumé Une étude de certains paramètres du poisson-chat de montagne Amphilius uranoscopus Pfeffer 1889 (Silurifomes: Amphiliidae) a été réalisée dans le courant du Thego, sur les pentes du mont Kenya entre février et décembre 2002. Le profil physique et chimique du Thego montre que les paramètres de la qualité de l'eau sont typiques des cours d'eau de haute altitude, avec une température qui dépasse rarement 18°C, un OD qui varie de 7,9 à 8,2 mg/l, et une conductivité relativement haute (87 à 137 ,S/cm) typique d'environnements lotiques perturbés. Au total, 1 010 poissons ont été capturés au moyen d'une canne électrique, d'une taille allant de 8 à 24 cm de longueur à la fourche. La structure de la population avait une distribution unimodale avec des maxima de 14,16 cm. La relation longueur/poids présente une variation relativement étroite dans la pente, allant de 2,61 en avril à 2,98 en février 2002, ce qui suggère un schéma de croissance isométrique. Le schéma de croissance intégré d'A. uranoscopus montre une longueur asymptotique (L,) de 28,5 cm et une courbure de croissance (K) de 0,56/an, résultant en un coefficient de mortalité naturelle estimé (M) de 0,90/an. Le facteur de condition de Fulton (K) était aussi relativement stable, avec un pic en avril (0,92 ± 0,21) et la valeur la plus basse en juin (0,86 ± 0,10). Puisque A. uranoscopus ne fait pas l'objet d'une exploitation commerciale, la dépression apparente de la population doit peut-être être attribuée à la truite arc-en-ciel, espèce exotique introduite qui se nourrit abondamment de l'espèce, et à des perturbations environnementales provoquées par des changements d'utilisation des terres. Les implications de tels changements pour A. uranoscopus sont discutées. [source]


The relationships between stocking density and welfare in farmed rainbow trout

JOURNAL OF FISH BIOLOGY, Issue 3 2002
T. Ellis
There is increasing public, governmental and commercial interest in the welfare of intensively farmed fish and stocking density has been highlighted as an area of particular concern. Here we draw scientific attention and debate to this emerging research field by reviewing the evidence for effects of density on rainbow trout. Although no explicit reference to ,welfare' has been made, there are 43 studies which have examined the effects of density on production and physiological parameters of rainbow trout. Increasing stocking density does not appear to cause prolonged crowding stress in rainbow trout. However, commonly reported effects of increasing density are reductions in food conversion efficiency, nutritional condition and growth, and an increase in fin erosion. Such changes are indicative of a reduced welfare status,although the magnitude of the effects has tended to be dependent upon study-specific conditions. Systematic observations on large scale commercial farms are therefore required, rather than extrapolation of these mainly small-scale experimental findings. There is dispute as to the cause of the observed effects of increasing density, with water quality deterioration and/or an increase in aggressive behaviour being variously proposed. Both causes can theoretically generate the observed effects of increasing density, and the relative contribution of the two causes may depend upon the specific conditions. However, documentation of the relationship between density and the effects of aggressive behaviour at relevant commercial densities is lacking. Consequently only inferential evidence exists that aggressive behaviour generates the observed effects of increasing density, whereas there is direct experimental evidence that water quality degradation is responsible. Nevertheless, there are contradictory recommendations in the literature for key water quality parameters to ensure adequate welfare status. The potential for welfare to be detrimentally affected by non-aggressive behavioural interactions (abrasion, collision, obstruction) and low densities (due to excessive aggressive behaviour and a poor feeding response) have been largely overlooked. Legislation directly limiting stocking density is likely to be unworkable, and a more practical option might be to prescribe acceptable levels of water quality, health, nutritional condition and behavioural indicators. [source]


ASSESSING LAND USE IMPACTS ON WATER QUALITY USING MICROBIAL SOURCE TRACKING,

JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION, Issue 6 2004
Sharon C. Long
ABSTRACT: A renewed emphasis on source water protection and watershed management has resulted from recent amendments and initiatives under the Safe Drinking Water Act and the Clean Water Act. Knowledge of the impact of land use choices on source water quality is critical for efforts to properly manage activities within a watershed. This study evaluated qualitative relationships between land use and source water quality and the quantitative impact of season and rainfall events on water quality parameters. High levels of specific conductance tended to be associated with dense residential development, while organic carbon was elevated at several forested sites. Turbidity was generally higher in more urbanized areas. Source tracking indicators were detected in samples where land use types would predict their presence. Coliform levels were statistically different at the 95 percent confidence levels for winter versus summer conditions and dry versus wet weather conditions. Other water quality parameters that varied with season were organic carbon, turbidity, dissolved oxygen, and specific conductance. These results indicate that land use management can be effective for mitigating impacts to a water body; however, year- round, comprehensive data are necessary to thoroughly evaluate the water quality at a particular site. [source]


WATER QUALITY IN AGRICULTURAL, URBAN, AND MIXED LAND USE WATERSHEDS,

JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION, Issue 6 2004
Chris B. Coulter
ABSTRACT: Water quality and nonpoint source (NPS) pollution are important issues in many areas of the world, including the Inner Bluegrass Region of Kentucky where urban development is changing formerly rural watersheds into urban and mixed use watersheds. In watersheds where land use is mixed, the relative contributions of NPS pollution from rural and urban land uses can be difficult to separate. To better understand NPS pollution sources in mixed use watersheds, surface water samples were taken at three sites that varied in land use to examine the effect of land use on water quality. Within the group of three watersheds, one was predominately agriculture (Agricultural), one was predominately urban (Urban), and a third had relatively equal representation of both types of land uses (Mixed). Nitrogen (N), phosphorus (P), total suspended solids (TSS), turbidity, pH, temperature, and streamflow were measured for one year. Comparisons are made among watersheds for concentration and fluxes of water quality parameters. Nitrate and orthophosphate concentrations were found to be significantly higher in the Agricultural watershed. Total suspended solids, turbidity, temperature, and pH, were found to be generally higher in the Urban and Mixed watersheds. No differences were found for streamflow (per unit area), total phosphorus, and ammonium concentrations among watersheds. Fluxes of orthophosphate were greater in the Agricultural watershed that in the Urban watershed while fluxes of TSS were greater in the Mixed watershed when compared to the Agricultural watershed. Fluxes of nitrate, ammonium, and total phosphorus did not vary among watersheds. It is apparent from the data that Agricultural land uses are generally a greater source of nutrients than the Urban land uses while Urban land uses are generally a greater source of suspended sediment. [source]


REEXAMINING BEST MANAGEMENT PRACTICES FOR IMPROVING WATER QUALITY IN URBAN WATERSHEDS,

JOURNAL OF THE AMERICAN WATER RESOURCES ASSOCIATION, Issue 5 2003
Stephen R. Pennington
ABSTRACT: Municipalities will be implementing structural best management practices at increasing rates in their effort to comply with Phase II of the National Pollutant Discharge Elimination System (NPDES). However, there is evidence that structural best management practices (BMPs) by themselves may be insufficient to attain desired water quality standards. This paper reports on an analysis of the median removal efficiencies of structural BMPs and compares them to removal efficiencies estimated as being necessary to attain water quality standards in the Rouge River in Detroit, Michigan. Eight water quality parameters are reviewed using data collected from 1994 to 1999 in the Rouge River. Currently, five of the eight parameters in the Rouge River including bacteria, biochemical oxygen demand, and total suspended solids (TSS) exceed the required water quality standards. The reported analysis of structural BMP efficiencies reveals that structural BMPs appear capable of reducing only some of the pollutants of concern to acceptable levels. [source]


Effects of Artificial Substrate and Stocking Density on the Nursery Production of Pacific White Shrimp Litopenaeus vannamei

JOURNAL OF THE WORLD AQUACULTURE SOCIETY, Issue 4 2004
Komarey R. K. Moss
Nursery production may be enhanced by the addition of artificial substrate to increase the surface area upon which shrimp graze and to serve as refuge. The objective of this study was to assess the effects of the artificial substrate, AquaMatsTM, on the performance of postlarval Pacific white shrimp Litopenaeus vannamei stocked at three densities. Eighteen 230-L tanks were stocked with 10-d postlarvae (mean weight < 0.01 g). Six treatments were evaluated and consisted of shrimp stocked at three densities (778 shrimp/m2, 1,167 shrimp/ m2, and 1,556 shrimp/m2) with and without access to artificial substrate. Shrimp in all treatments received a commercial diet ad libitum. After 6 wk, shrimp were harvested from each nursery tank, counted, and batch weighed. Mean final weight, survival, production, feed conversion ratio, and water quality parameters were analyzed by 2-way ANOVA. There were highly significant (P < 0.001) density and substrate effects on final weight, but there was no significant interaction effect. Final weight was 26.0, 17.4, and 34.5% greater in treatments with substrate than without substrate when stocked at 778, 1,167, and 1,556 shrimp/m2, respectively. There was no significant density, substrate, or interaction effect on survival or water quality. Mean survival was ± 89.1% for all treatments. Increased shrimp growth in the presence of added substrate was likely due to the availability of attached particulate organic matter on the AquaMatsTM that served as an additional food source. Results from this study indicate that artificial substrate can be used to mitigate the potential negative effects of high stocking density on growth of L. vannamei in nursery systems. [source]


Density and Water Exchange-Dependent Growth and Survival of Litopenaeus setiferus Postlarvae

JOURNAL OF THE WORLD AQUACULTURE SOCIETY, Issue 2 2001
Gabriela Palomino
This present study was designed to investigate the effects of stocking density and water exchange on the growth rate, survival and performance index of L. setiferus postlarvae under controlled laboratory conditions. The experiment was done with postlarvae (PL10 to PL40) at densities of 50, 150, 250 and 350 shrimp/m2 and various different water exchanges rate per day (0, 6, 12 and 18%). The maximum growth rate was obtained for shrimp with 12% water exchange per day at all densities. A reduction of the maximum growth rate was observed in relation to density with the highest values in shrimp stocked in a density of 50 and 150 shrimp/m2 (mean value of 0.53 mg/d) and the lowest in shrimp stocked in a density of 350 shrimp/m2 (0.24 mg/d). The multiple regression equation obtained to relate performance index (growth rate* survival : PI), shrimp density (X1) and water exchange (X2) was: PI = 0.31 + (0.001) X1+ 0.039 X2+ 2.28 × 10,6 X12+ (,0.0017) X22+ (0.000026)X1X2,R2= 0.78; P > 0.03. According to this equation the optimum shrimp density-water exchange comhination was between 5 to 12% of water exchange at stocking density of between 50 and 150 shrimp/m2. Salinity, ammonia-N and nitrite-N increased according to the time spent in tanks without water exchange. With no (0%) water exchange, water quality parameters measured were outside the optimum for L. setiferus postlarvae. The use of optimum density and water exchange in a nursery system for L. setiferus with optimum variables established is proposed. [source]


Toxic effect of environmental acid-stress on the sperm of a hill-stream fish Devario aequipinnatus: A scanning electron microscopic evaluation

MICROSCOPY RESEARCH AND TECHNIQUE, Issue 2 2009
Sudip Dey
Abstract Environmental stress due to acidic pH of water was found to be one of the major factors leading to toxic effects on the sperm of a hill-stream fish Devario aequipinnatus of Meghalaya, India. The Scanning Electron Microscopy of the transverse section of testes of the fish collected from its natural habitat with acidic pH (5.6,6.0) showed that the sperms were clumped together and their tails were either absent or were of extremely small length. The acrosome and midpiece were also not well differentiated. When the fingerlings from the natural habitat were reared to maturity in aquarium with water from natural habitat after changing the pH to alkaline range (8.0,8.2), the clumping of the sperm was not observed. The sperm tail was found to be well-developed along with well-differentiated acrosome and midpiece. Since the only change in the water quality parameters of the experimental aquarium as compared to those of the natural habitat was the pH, it is evident that the abnormal features of the sperm observed in fish from natural habitat is mainly because of environmental acid stress. Microsc. Res. Tech., 2009. © 2008 Wiley-Liss, Inc. [source]


Density effects of silver carp Hypophthalmichthys molitrix and catla Catla catla on the production system in all-male freshwater prawn,finfish polyculture ponds

AQUACULTURE RESEARCH, Issue 10 2010
Sheikh Md.
Abstract The effects of three different combinations of silver carp Hypophthalmichthys molitrix and catla Catla catla density on the production system in all-male freshwater prawn,finfish polyculture ponds were evaluated in triplicate. The stocking density of silver carp and catla, respectively, were maintained at 2000 and 500 ha,1 in treatment SC2000C500, 1500 and 1000 ha,1 in treatment SC1500C1000 and 1000 and 1500 ha,1 in treatment SC1000C1500. Male freshwater prawn Macrobrachium rosenbergii and small fish mola Amblypharyngodon mola densities were fixed in all treatments at 12 000 and 20 000 ha,1 respectively. Management practices were same for all treatments. Blue-clawed male prawns were harvested twice during the 122-day culture at 15-day intervals before the final harvest. Plankton and macro-benthos abundance and water quality parameters (except transparency and chlorophyll a) did not vary significantly (P>0.05) among treatments. Mean final weights of both silver carp and catla were decreased with the increasing of their own stocking density. The treatment SC1500C1000 resulted in 25,32% increased net production of silver carp plus catla (461 kg ha,1) and 20,21% increased net production of all species combined (874 kg ha,1) as compared with the other treatments, although the differences in production of prawn and mola among treatments were not significant. [source]


Probiotic effect of Bacillus NL110 and Vibrio NE17 on the survival, growth performance and immune response of Macrobrachium rosenbergii (de Man)

AQUACULTURE RESEARCH, Issue 9 2010
K M Mujeeb Rahiman
Abstract Eight hundred and eighty-five strains of bacterial isolates from various samples associated with the natural habitat of Macrobrachium rosenbergii were screened for their probiotic potential. Two putative probionts namely Bacillus NL110 and Vibrio NE17 isolated from the larvae and egg samples, respectively, were selected for experimental studies and were introduced to the juveniles of M. rosenbergii (0.080±0.001 g) through different modes such as through feed, water and both. The probiotic potential of the above bacteria in terms of improvements in water quality, growth, survival, specific growth rate (SGR), feed conversion ratio and immune parameters was evaluated. The treatment groups showed a significant improvement in SGR and weight gain (P<0.001). Survival among different treatment groups was better than that in the control group. There were also significant improvements in the water quality parameters such as the concentration of nitrate and ammonia in the treatment groups (P<0.05). Improvements in immune parameters such as the total haemocyte count (P<0.05), phenoloxidase activity and respiratory burst were also significant (P<0.001). It is concluded that screening of the natural microflora of cultured fish and shellfish for putative probionts might yield probiotic strains of bacteria that could be utilized for an environment-friendly and organic mode of aquaculture. [source]


Effects of the probiotic, Lactobacillus acidophilus, on the growth performance, haematology parameters and immunoglobulin concentration in African Catfish (Clarias gariepinus, Burchell 1822) fingerling

AQUACULTURE RESEARCH, Issue 14 2009
Mohammed Abdullah Al-Dohail
Abstract This experiment was carried out to evaluate the effects of the probiotic, Lactobacillus acidophilus, on the growth performance, haematology parameters and immunoglobulin concentration in African catfish Clarias gariepinus fingerling. Two experimental diets were formulated to contain 35 g kg,1 crude protein and 10 g kg,1 lipids accordingly and fed three times daily for 12 weeks to 25 C. gariepinus fingerlings per fibreglass tank in 12 replicates each. The control diet was prepared with no probiotic supplementation whereas the second diet was prepared supplemented with a probiotic, L. acidophilus, containing about 3.01 × 107 colonies/g of diet. The results show that growth performance [specific growth rate (SGR) and relative growth rate (RGR)], nutrient utilization [protein efficiency ratio (PER) and feed conversion ratio (FCR)] and survival were significantly (P<0.05) higher in fish maintained on the probiotic-supplemented diet compared with those on the control diet. Haematology parameters (packed cell volume, haemoglobin, erythrocyte sedimentation rate, red blood cell and white blood cell, total serum protein, Ca2+, Mg2+, Cl,, glucose and cholesterol) and total immunoglobulin concentrations were also significantly better in fish fed the probiotic-supplemented diet than in the control. Although the water quality parameters monitored were better in the fish fed the probiotic-supplemented diet than in the control, the parameters were not significantly different (P>0.05). From the results of this experiment, we conclude that L. acidophilus can be used as a probiotic agent in African catfish culture, to enhance fish health, survival and better feed efficiency and growth performance. [source]


Growth performance, survival and maturation of Litopenaeus vannamei (Boone) in an inland CRS with no water reposition

AQUACULTURE RESEARCH, Issue 12 2009
Abundio González-González
Abstract Closed recirculation systems (CRS) present an alternative for providing organisms to the aquaculture. A CRS with zero water exchange was used in the present study; the CRS consisted of a culture and maturation facility, biofilter system and reservoirs tanks. During two consecutive trials, the CRS efficiency was evaluated by assessing the growth, survival and maturation of juveniles into adults Litopenaeus vannamei (Boone). Throughout the study, water quality parameters (temperature, pH, salinity, NH3, NO2 and NO3) and ion concentration were monitored. Most parameters showed fluctuations without significant differences. However, a decrease in pH was observed during the maturation phase, and an increase in phosphorus was detected, in both the trials, compared with that in initial seawater. Growth and survival for juvenile and pre-adult shrimps presented similar variations without significant differences. Female gonadal maturation and spawning rate were not significantly different between trials. Unfortunately, shrimp eggs underwent lysis 6 h after spawning. These results show that the growth, survival and maturation obtained under CRS conditions are reproducible, and suggest the possibility of using these systems for biosecure shrimp culture, protected against eventual diseases outbreaks. The results of this study also suggest the importance of future studies addressing ion concentration changes in a CRS with zero water exchange. [source]


Introduction of jundia Rhamdia quelen (Quoy & Gaimard) and Nile tilapia Oreochromis niloticus (Linnaeus) increases the productivity of carp polyculture in southern Brazil

AQUACULTURE RESEARCH, Issue 5 2008
Leonardo Bolognesi Da Silva
Abstract Fish polyculture is based on the assumption that each species has its own feeding niche and may increase the maximum standing crop of a pond by exploring a wider range of available food and ecological niches. In order to identify the better species ratio and to introduce jundia (JN) (Rhamdia quelen Quoy & Gaimard) and Nile tilapia (NT) (Oreochromis niloticus Linnaeus) in to the carp polyculture practiced in South Brazil, a 162-day experiment was conducted, in 12 250-m2 earthen ponds (1.2 m deep). Treatment I (T-I) contained 35% common carp, Cyprinus carpio (L.) (CC); 35% grass carp, Ctenopharyngodon idella Valenciennes (GC); 15% silver carp, Hypophthalmichthys molitrix Valenciennes (SC); and 15% bighead carp, Aristichthys nobilis Richardson (BC). Treatment II (T-II) consisted of three ponds stocked at the following ratio: 17.5% CC, 35% GC, 15% SC, 15% BC and 17.5% JN. Treatment III (T-III) consisted of three ponds with 35% CC, 35% GC, 7.5% SC, 7.5% BC and 15% NT. Treatment IV (T-IV) consisted of three ponds with 17.5% CC, 35% GC, 7.5% SC, 7.5% BC%, 17.5% JN and 15% NT. No significant correlation was found between the treatments with different species ratio and water quality parameters. The final weight of different species, in different treatments, was statistically different. The major result was the clear positive effect on growth parameters observed by the introduction of JN and/or NT in to the carp polyculture. The yield per hectare was 2083.33±183 kg ha,1 for polyculture with carp species; 2476.67±139.88 kg ha,1 following the introduction of JN only; 2801.67±111.42 kg ha,1 for isolated introduction of NT; and 2506.67±422.31 kg ha,1 for simultaneous introduction of JN and NT. The introduction of JN and/or NT had a positive effect on growth parameters when compared with carp-only polyculture. The reduction in CC ratio also had a positive effect on growth parameters. [source]


Low-cost diet for monoculture of giant freshwater prawn (Macrobrachium rosenbergii de Man) in Bangladesh

AQUACULTURE RESEARCH, Issue 3 2007
Md Arshad Hossain
Abstract An experiment was conducted for 3 months in 12 experimental ponds, each of 30 m2, with a view to develop a low-cost diet for monoculture of Macrobrachium rosenbergii in ponds. Three experimental diets (30% protein) were formulated using fish meal, meat and bone meal, mustard oilcake, sesame meal and rice bran in different combinations partially replacing fish meal by meat and bone meal and sesame meal and assigned to treatments T1, T2 and T3 respectively. A commercial golda feed (Starter-III) was assigned to T4 (reference diet). Each treatment had three replicates. Juveniles of M. rosenbergii (2.90±0.21 g) were stocked at the rate of 40 000 ha,1. Prawns were fed three times daily at the rate of 10% and 5% of their body weight at the beginning and for the last 2 months respectively. The ponds were provided with aeration during the night using air pumps. The ranges of water quality parameters recorded in different ponds were: temperature 28.9,32.5°C, dissolved oxygen 5.1,8.1 mg L,1 and pH 6.4,7.7. The results showed that the weight gain of prawns fed diet 1 was significantly higher (P<0.05) than those fed diets 2 and 3, but was not significantly different from those fed diet 4 (reference diet). The feed conversion ratio (FCR) values of diets ranged between 2.21 and 2.96 with diets 1 and 4 showing significantly lower (P<0.05) FCR values. The survivals (%) ranged between 68% and 78% with prawns fed diets 1 and 4 showing significantly higher survival. The production of prawn ranged between 921 and 1428 kg ha,1 and diet 1 resulted in a significantly high (P<0.05) production. A simple economic analysis showed that diet 1 generated the maximum net profit of Tk 159 178 ha,1. The results of the study showed that a diet containing 20% fish meal, 10% meat and bone meal, 15% mustard oilcake, 15% sesame meal, 35% rice bran, 4% molasses and 1% vitamin,mineral premixes may be recommended to the farmers for monoculture of M. rosenbergii in ponds. [source]


Effect of dietary protein level on growth, survival and ammonia efflux rate of Litopenaeus vannamei (Boone) raised in a zero water exchange culture system

AQUACULTURE RESEARCH, Issue 9 2005
Silvia Gómez-Jiménez
Abstract Litopenaeus vannamei postlarvae (1.96±0.07 g) were reared in a zero water exchange system for 25 days at 28°C. They were fed four commercial diets containing 25%, 30%, 35% or 40% crude protein in three replicate aquaria per dietary treatment. Total ammonia, nitrite, nitrate and pH were monitored weekly and total ammonia levels were additionally measured every 3 days using the flow injection analysis method. Total ammonia efflux rates were measured at days 0, 14 and 21, and survival and growth rates were recorded at the end of the experiment. No significant differences between water quality parameters such as temperature, salinity, dissolved oxygen and pH were found. Nitrite concentration remained low in all dietary treatments up to the second week increasing considerably from day 14 onwards suggesting the initiation of the nitrification process. Water total ammonia of all experimental groups exhibited a gradual increase up to day 13; however, following this time ammonia levels of all experimental groups decreased, probably due to either the action of bacterial nitrification or ammonia-N uptake by the animals. High ammonia efflux rates were recorded at day 14, especially after the first hour of immersion in the 25% protein group, but no significant changes occurred in any experimental group after 3 h. No significant differences in weight gain, final weight or survival of shrimp were observed under these experimental conditions. The importance of zero water exchange systems and their effects on the nitrogen metabolism of crustaceans are discussed. [source]


Promotion and contribution of biota in low water exchange ponds farming blue shrimp Litopenaeus stylirostris (Stimpson)

AQUACULTURE RESEARCH, Issue 1 2002
Luis R Martinez-Cordova
Abstract An experimental study was conducted during 20 weeks in Bahia Kino, Sonora, Mexico, in order to evaluate the feasibility of promoting biota in low-water exchange ponds farming blue shrimp, Litopenaeus stylirostris. The effect of that promotion on the production parameters of cultured shrimp as well as on the water quality parameters was evaluated. Treatments consisted of: (i) ponds fed formulated food (FF), and (ii) ponds fed formulated plus promoted natural food (NFF). Phytoplankton, zooplankton and benthos were effectively promoted during some weeks of the culture period. Growth and feed conversion ratio (15.16 g and 1.79 respectively) were significantly better in treatment NFF than in treatment FF (13.89 g and 2.02 respectively). Differences in some of the water quality parameters were observed among treatments. Phosphates (0.15 mg/L versus 0.53 mg/L), and total ammonia-N (0.09 mg/L versus 0.12 mg/L) presented greater concentrations in treatment FF than in the NFF. [source]